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Zhang Lab - Multiplexed Overexpression of Regulatory Factors (MORF) Library
(Pooled Library #1000000218, #192821)

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  • Purpose

    The Multiplexed Overexpression of Regulatory Factors (MORF) pooled library is a comprehensive collection of human transcription factor open reading frames. Each isoform is uniquely barcoded with a 24 bp sequence, to simplify factor identification during screening. Individual plasmids are also available on the MORF Collection page.

  • Vector Backbone

    pLX_TRC317 - Constructed by inserting ORF and barcode in pLEX_307

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 1000000218 MORF library with GFP and mCherry controls + 2 control plasmids
  • #137000 (MORF library with GFP and mCherry controls)
  • #145025 (TFORF3549 GFP)
  • #145026 (TFORF3550 mCherry)
 1 $430 Add to Cart
Pooled Library 192821 MORF library without GFP and mCherry controls 1 $380 Add to Cart
Available to Academic and Nonprofits Only

Library Details

  • Species
    Human
  • ORFs
    3,548 isoforms of 1,836 genes
  • Insert size range
    250 - 10,000 bp
  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

For the Kit with GFP and mCherry controls ONLY: You will also receive bacterial stabs of TFORF3549 (GFP, Addgene ID #145025) and TFORF3550 (mCherry, Addgene ID #145026) plasmids. Please do NOT order these plasmids in addition to this library.

  • Volume
    ∼20 µl
  • Concentration
    50 ng/µL

Resource Information

Depositor Comments

The Pooled library #137000 contains low levels of the TFORF3549 (GFP) and TFORF3550 (mCherry) control plasmids since cells that express the control plasmids will grow faster than cells with differentiated phenotypes in a pooled screen. The individual GFP and mCherry plasmids are also provided as bacterial agar stabs in the kit. If you have difficulty amplifying the control plasmids or if you would like higher amounts of mCherry or GFP, you can choose to add in more control plasmids separately, after library amplification. For example, add 3 ng of each control plasmid (GFP and mCherry) to 10 ug of the amplified pooled library.


For library transformation and amplification, please refer to Joung et al. Nat Protoc. 2017, with 2 modifications:

  • 1. As this is an ORF library, increasing the coverage (i.e., colonies/ORF) to 5-10 electroporations is recommended to minimize skewing.
  • 2. Plate 1 electroporation onto 2 large LB agar plates to help minimize colony contact.

A diagram of lentiviral plasmids arrayed in a multi-well plate, labeled “arrayed ORF library”. To the right is an image of lentiviral plasmids inducing cellular differentiation into different cell types.

  • Multiplexed Overexpression of Regulatory Factors (MORF) Library

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Multiplexed Overexpression of Regulatory Factors (MORF) Library was a gift from Feng Zhang (Addgene #1000000218)
    Multiplexed Overexpression of Regulatory Factors (MORF) with GFP and mCherry controls Library was a gift from Feng Zhang (Addgene #137000)
    Multiplexed Overexpression of Regulatory Factors (MORF) TFORF3549 GFP Library was a gift from Feng Zhang (Addgene #145025)
    Multiplexed Overexpression of Regulatory Factors (MORF) TFORF3550 mCherry Library was a gift from Feng Zhang (Addgene #145026)
    Multiplexed Overexpression of Regulatory Factors (MORF) without GFP and mCherry controls Library was a gift from Feng Zhang (Addgene #192821)

  • For your References section:

    A transcription factor atlas of directed differentiation. Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F Cell. 2023 Jan 5; 186(1):209-229. doi: 10.1016/j.cell.2022.11.026.
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