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Dow Lab Base Editing Sensor Libraries
(Pooled Library #179217, #179218, #179219)

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  • Purpose

    The Mouse Base Editing Sensor (MBES) and Human Base Editing Sensor (HBES) libraries are made up of sgRNAs that target frequently occurring cancer-associated mutations identified in the MSK IMPACT dataset that can be modeled with cytosine base editing. The ALL-PAM sensor (AP) library is made up of 10 human and mouse sgRNAs, where for each sgRNA the tethered target site has 1 of 64 possible 3-bp PAM sequences. Notably these "sensor libraries" also contain the cognate genomic target site within the same expression cassette for quantification of base editing efficiency.

  • Vector Backbone

    pLenti-U6-tdTomato-P2A-BlasR (LRT2B) (Plasmid #110854) - does not express base editor

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 179217 Mouse Base Editing Sensor Library† 1 $380 Add to Cart
Pooled Library 179218 Human Base Editing Sensor Library† 1 $380 Add to Cart
Pooled Library 179219 ALL-PAM sensor Library† 1 $380 Add to Cart
Available to Academic and Nonprofits Only

Library Details

Library Species Genes Targeted No. gRNAs
MBES Mouse 462 4,686
HBES Human 462 5,855
All-PAM Mouse & Human 20 1,280

  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼20 µL
  • Concentration
    50 ng/µL

Resource Information

Depositor Comments

Explore the library targets and browse guide RNAs by species, gene, target mutation, and/or base editor at the BE-SCAN web application (Link opens in a new window). The base editor sensor libraries were generated using the AMINEsearch pipeline available on GitHub (Link opens in a new window).

A script for analyzing base-editing sensor outcomes is available on GitHub (Link opens in a new window).

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mouse Base Editing Sensor Library was a gift from Lukas Dow (Addgene #179217)
    Human Base Editing Sensor Library was a gift from Lukas Dow (Addgene #179218)
    ALL-PAM Sensor Library was a gift from Lukas Dow (Addgene #179219)

  • For your References section:

    Base editing sensor libraries for high-throughput engineering and functional analysis of cancer-associated single nucleotide variants. Sánchez-Rivera FJ, Diaz BJ, Kastenhuber ER, Schmidt H, Katti A, Kennedy M, Tem V, Ho Y-J, Leibold J, Paffenholz SV, Barriga FM, Chu K, Goswami S, Wuest AN, Simon JM, Tsanov KM, Chakravarty D, Zhang H, Leslie CS, Lowe SW, Dow LE. Nat Biotechnol. 2022 Feb 14. doi: 10.1038/s41587-021-01172-3. PMID 35165384
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