Chen Mouse Metastasis CRISPR-Cpf1(Cas12a) Double Knockout Library
(Pooled Library #123361)

• Purpose:

  This lentiviral library is a CRISPR-Cpf1/Cas12a double knockout library. There are 26 genes targeted in the library, chosen from analysis of human metastasis cohorts and a prior mouse metastasis CRISPR screen. It can be used to interrogate the genetic interactions driving metastasis.

• Vector Backbone:

  pRG01-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-WPRE (Plasmid #123362) - does not express Cpf1/Cas12a

• Depositing Labs: Sidi Chen
• Publication: Chow et al. Nat Methods. 2019 May;16(5):405-408. doi: 10.1038/s41592-019-0371-5.

Library Details

• Species: Mouse
• Genes targeted: 26
• crRNAs: 104
• Controls: 52 non-targeting
• Lentiviral Generation: 3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

• Volume: ∼10 µL
• Concentration: 50 ng/µL

Resource Information

• Protocols:
  • Amplification & Sequencing Protocol
• Depositor Data:
  • Annotated GenBank Library Backbone (GB, 17 KB)
  • List of crRNA sequences (XLSX, 741 KB)
  • NGS Read Counts (TXT, 188 KB)
  • Distribution of NGS Reads (PDF, 51 KB)

Terms and Licenses

Academic/Nonprofit Terms:

• UBMTA
• Luciferase Limited Use Label License

Industry Terms:

• Not Available to Industry

Trademarks:

• Zeocin® is an InvivoGen trademark.

Depositor Comments

26 unique genes are targeted, corresponding to 325 unique gene pairs. Each gene has 4 targeting crRNAs. Combined with 52 non-targeting crRNAs, this means there are 1,326 NTC-NTC dual-crRNA arrays, 5,408 single knockout dual-crRNA arrays (208 per gene), and 5,200 double knockout dual-crRNA arrays (16 per gene pair).