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Mouse Lipid and Sphingolipid Metabolism Knockout Libraries
(Pooled Library #206248, #206249)

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  • Purpose

    The Mouse Lipid Metabolism Library contains guide RNAs against 295 mouse genes encoding enzymes that directly impact lipid metabolism. The Mouse Sphingolipid Metabolism Library targets 59 mouse genes in the de novo ceramide and downstream sphingolipid metabolism pathways. 40 targets from the Lipid Metabolism Library are also targeted in the Sphingolipid Metabolism Library. Both of these libraries can be used to find lipid metabolic vulnerabilities in mouse cells.

  • Vector Backbone

    LentiCRISPRv2-Opti - expresses Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 206248 Mouse Lipid Metabolism Knockout 1 $430 Add to Cart
Pooled Library 206249 Mouse Sphingolipid Metabolism Knockout 1 $430 Add to Cart
Available to Academic and Nonprofits Only

Library Details

  • Species
    Mouse
  • Lentiviral Generation
    3rd
    • Genes/elements targeted gRNAs per element Control gRNAs Total gRNAs
    Lipid Metabolism Knockout Library 295 7 40 2,105
    Sphingolipid Metabolism Knockout Library 59 10 40 630

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼20 µL
  • Concentration
    25 ng/µL

Resource Information

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Birsoy Mouse Lipid Metabolism Knockout Pooled Library was a gift from Kivanc Birsoy (Addgene #206248)
    Birsoy Mouse Sphingolipid Metabolism Knockout Pooled Library was a gift from Kivanc Birsoy (Addgene #206249)

  • For your References section:

    Glycosphingolipid synthesis mediates immune evasion in KRAS-driven cancer. Soula M, Unlu G, Welch R, Chudnovskiy A, Uygur B, Shah V, Alwaseem H, Bunk P, Subramanyam V, Yeh HW, Khan A, Heissel S, Goodarzi H, Victora GD, Beyaz S, Birsoy K. Nature. 2024 Aug 7. doi: 10.1038/s41586-024-07787-1. PubMed 39112706
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