Skip to main content
Addgene

Human Metabolic Gene CRISPRa sgRNA Library
(Pooled Library #187080)

Print
  • Purpose

    This lentiviral CRISPRa library targets 2,989 human metabolic genes for activation of gene expression.

  • Vector Backbone

    lentiGuide-Puro (Plasmid #52963) - does not express Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 187080 Human Metabolic Gene CRISPRa sgRNA Library† 1 $540 Add to Cart
Available to Academic and Nonprofits Only

  • † A Cas9 plasmid is NOT included with this item and will have to be ordered separately. The depositing lab suggests using a CRISPRa dCas9-VPR vector with an hEF1a promoter from Horizon Discovery, catalog number CAS11916.

Library Details

  • Species
    Human
  • Genes targeted
    2,989 metabolic genes
  • gRNAs
    32,460
  • Controls
    49 non-targeting sgRNAs
  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    20 µL
  • Concentration
    25 ng/µL

Resource Information

Depositor Comments

Please see the publication (Unlu et al. 2022) for more detailed information. The preprint is also available on bioRxiv (Link opens in a new window).

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human Metabolic Gene CRISPRa sgRNA Library was a gift from Kivanç Birsoy (Addgene #187080)

  • For your References section:

    Metabolic-scale gene activation screens identify SLCO2B1 as a heme transporter that enhances cellular iron availability. Unlu G, Prizer B, Erdal R, Yeh HW, Bayraktar EC, Birsoy K. Mol Cell. 2022 Aug 4;82(15):2832-2843.e7. doi: 10.1016/j.molcel.2022.05.024. PubMed 35714613
Related items
Additional resources