Endy and Shearwin Lab pOSIP Plasmid Kit
(Kit #
1000000035
)
Depositing Labs:
Drew Endy,
Keith Shearwin
This kit contains plasmids for use in “clonetegration”, a method for integrating DNA into prokaryotic chromosomes that approaches the simplicity of cloning DNA within extrachromosomal vectors. Compared to existing techniques, clonetegration drastically decreases the time and effort needed for integration of single or multiple DNA fragments. Additionally, clonetegration facilitates cloning and expression of genetic elements that are impossible to propagate within typical multi-copy plasmids.
This kit consists of 11 bacterial stabs and will be shipped at room temperature.
Original Publication
One-Step Cloning and Chromosomal Integration of DNA. St-Pierre F, Cui L, Priest DG, Endy D, Dodd IB, Shearwin KE. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20 - PubMed
Additional References
A detailed description, protocol and troubleshooting tips for using the OSIP plasmids can be found in the following publication:
Clonetegration Using OSIP Plasmids: One-Step DNA Assembly and Site-Specific Genomic Integration in Bacteria. Lun Cui and Keith E. Shearwin. Methods in Molecular Biology, 2017;1472:139-55, DOI: 10.1007/978-1-4939-6343-0_11. PubMed
How to Cite this Kit
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.
For your Materials and Methods section:
"The pOSIP plasmid kit used for clonetegration was a gift from Drew Endy and Keith Shearwin (Addgene kit # 1000000035)"
For your Reference section:
One-Step Cloning and Chromosomal Integration of DNA. François St-Pierre, Lun Cui, David G. Priest, Drew Endy, Ian B. Dodd, and Keith E. Shearwin. ACS Synth. Biol., Article ASAP, DOI : 10.1021/sb400021j Publication Date (Web): May 6, 2013. -
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Kit Contents
This set consists of these plasmids:
| ID | Name |
|---|---|
| 45978 | pE-FLP |
| 45980 | pOSIP-CH (CamR, HK022) |
| 45981 | pOSIP-CT (CamR, P21) |
| 45982 | pOSIP-KC (KanR, PhiC31) |
| 45983 | pOSIP-KH (KanR, HK022) |
| 45984 | pOSIP-KL (KanR, Lambda) |
| 45985 | pOSIP-KO (KanR, 186) |
| 45986 | pOSIP-KP (KanR, Phi80) |
| 45987 | pOSIP-KT (KanR, P21) |
| 45988 | pOSIP-TH (TetR, HK022) |
| 45989 | pOSIP-TT (TetR, P21) |