Mobius Assembly Vector Toolkit
(Kit #
1000000134
)
Depositing Lab: Naomi Nakayama
The Mobius Assembly Toolkit is a new Golden Gate Assembly framework, which combines vector toolkit simplicity with high cloning capacity. It is based on a two-level, hierarchical approach and utilizes a low-frequency cutter to reduce domestication requirements. Mobius Assembly embraces the standard overhang designs designated by MoClo, Golden Braid, and Phytobricks and is largely compatible with already available Golden Gate part libraries.
This kit will be shipped as bacterial glycerol stocks in 96-well plate format.
Original Publication
Mobius Assembly: A versatile Golden-Gate framework towards universal DNA assembly. Andreou AI, Nakayama N (2018) PLOS ONE 13(1): e0189892. PMID: 29293531 .
Description
Mobius Assembly vector toolkit consists of 16 plasmids: one mUAV, four Level 1 Acceptor Vectors, four Level 2 Acceptor Vectors and seven Auxiliary Plasmids. Mobius Assembly is a Golden Gate Assembly framework which renders both high cloning capacity and vector toolkit simplicity. It is based on a two-level approach which enables a quadruple hierarchical assembly augmentation, it has reduced domestication requirements and employs a chromogenic, visible cloning screening.
Mobius Assembly uses a two-level approach for transcriptional unit (TU) and multi-TU augmentation. Each level is comprised of four Acceptor Vectors. The standard parts stored in mUAVs are released and fused in a Level 1 reaction to form a TU. Up to four Level 1 TUs can be fused in a Level 2 reaction to form a multi-TU cassette. Switching back and forth between Level 1 and 2 leads to further expansion of multi-TUs according to the geometric sequence: 1, 4, 16, 64, ...
Kit Documentation
Supplemental document:
Mobius_Assembly_Supporting_Materials_and_Protocols.pdf 397.6 KBHow to Cite this Kit
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.
For your Materials and Methods section:
“The Mobius Assembly Vector Toolkit was a gift from Naomi Nakayama (Addgene kit # 1000000134).”
For your Reference section:
Mobius Assembly: A versatile Golden-Gate framework towards universal DNA assembly. Andreou AI, Nakayama N (2018) PLOS ONE 13(1): e0189892. PMID: 29293531 .
Mobius Assembly Vector Toolkit - #1000000134
- Resistance Color Key
Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.
- Inventory
Searchable and sortable table of all plasmids in kit. The Well column lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.
- Kit Plate Map
96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.
Resistance Color Key
| Chloramphenicol | |
| Kanamycin |
Inventory
| Well | Plasmid | Resistance |
|---|---|---|
| A / 1 | mUAV | Chloramphenicol |
| A / 2 | pMA_Level 1A | Kanamycin |
| A / 3 | pMA_Level 1B | Kanamycin |
| A / 4 | pMA_Level 1Γ | Kanamycin |
| A / 5 | pMA_Level 1Δ | Kanamycin |
| A / 6 | pMA_Level 2A | Chloramphenicol |
| A / 7 | pMA_Level 2B | Chloramphenicol |
| A / 8 | pMA_Level 2Γ | Chloramphenicol |
| A / 9 | pMA_Level 2Δ | Chloramphenicol |
| A / 10 | pMA_Auxiliary 1 | Kanamycin |
| A / 11 | pMA_Auxiliary 2 | Kanamycin |
| A / 12 | pMA_Auxiliary 3 | Kanamycin |
| B / 1 | pMA_Auxiliary 4A | Kanamycin |
| B / 2 | pMA_Auxiliary 4B | Kanamycin |
| B / 3 | pMA_Auxiliary 4Γ | Kanamycin |
| B / 4 | pMA_Auxiliary 4Δ | Kanamycin |