Fire Lab C. elegans Vector Kit
(Kit #
1000000001
)
Depositing Labs: Andrew Fire
Dr. Andrew Fire's lab has deposited a set of vectors for C. elegans research. This kit contains 288 vectors and includes both a general description and the full predicted DNA sequence for each vector.
This kit consists of 3 plates (288 plasmids), and will be shipped as bacterial glycerol stocks, 96-well format, on dry ice. Samples should be frozen at -80°C immediately upon arrival. Individual plasmids can be ordered from each plasmid page and will be shipped as bacterial stabs. Addgene does not sell worm strains.
Description
Dr. Andrew Fire's lab has deposited a set of vectors for C. elegans research. This kit contains 288 vectors and includes both a general description and the full predicted DNA sequence for each vector. Among these vectors are:
lacZ and/or GFP fusion vectors with higher expression efficiency than many earlier C. elegans vectors. These include vectors with coding region for blue-shifted GFP, cyan-shifted GFP, yellow-shifted GFP, and other GFP protein variants.
Vectors for tagging C. elegans exons and introns with GFP.
Control plasmids for RNA-mediated interference.
Hairpin sequences to test promoters for ability to trigger RNAi in-vivo.
Kit Documentation
Fire Lab C. elegans Vector Kit Info Addgene 238.8Â KB
The vectors in this kit are divided into 3 plates (96 samples each). The sample plate location information and documentaion are as follows:
- 1995 plate (blue colored caps)
- 1997 plate (yellow colored caps)
- 1999 plate (green colored caps)
- 2005 vectors: H1 through H12 of the 1995 plate, H3 through H12 of the 1999 plate, and E8 and F7 of the 1999 plate are 24 new vectors made available in 2005. These vectors were not available in the 1995 and 1999 vector sets previously distributed by the Fire lab.
Additional documents from the Fire lab:
More Downloadable Documentation
In these kit documentations, plasmids may be referred by their mini-prep number (prefix pPD), or ligation number (prefix L). Please consult the Excel file below for translation between these naming schemes. In the Addgene library, you may search for a plasmid using either number.
- Fire Lab C. elegans Vector Kit Datasheets and Maps [.pdf] 7.7Â MB
- Fire Lab C. elegans Vector Kit Plasmid Features Sequences Plate Locations [.xls] 2.6Â MB
- Fire Lab C. elegans Vector Kit Data [.zip] 743.7Â KB
- Fire Lab C. elegans Vector Kit GenBank Sequences [.txt] 2.9Â MB
-
Fire Lab C. elegans Vector Kit Plasmid Info DNA Janitor [.bin]
473.4Â KB
Plasmid information and sequences are also packaged into a Hypercard application called DNA Janitor (472 KB zip/StuffIt). DNA Janitor, written by Dr. Fire, provides interfaces for browsing and searching plasmid information. This application requires a HyperCard viewer from Apple to run on either MacOS X Classic Mode, or MacOS 9 or earlier.
How to Cite this Kit
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.
For your Materials and Methods section:
"The Fire Lab C. elegans Vector Kit was a gift from Andrew Fire (Addgene kit # 1000000001)"
Note that Plate 1 listed on this page corresponds to Fire Lab C. Elegans Vector Kit 1995, Plate 2 corresponds to Kit 1997 and Plate 3 corresponds to Kit 1999
Fire Lab C. Elegans Kit - #1000000001
- Resistance Color Key
Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.
- Inventory
Searchable and sortable table of all plasmids in kit. The Well column links to its kit plate map on the right, as well as lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.
- Kit Plate Map
96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.
Resistance Color Key
| Ampicillin | |
| Kanamycin |
Inventory
| Well | Plasmid | Resistance |
|---|---|---|
| Plate 1 / A / 1 | pPD87_18 | Ampicillin |
| Plate 1 / A / 2 | pPD90_23 | Ampicillin |
| Plate 1 / A / 3 | pPD95_67 | Ampicillin |
| Plate 1 / A / 4 | pPD80_08 | Ampicillin |
| Plate 1 / A / 5 | pPD88_22 | Ampicillin |
| Plate 1 / A / 6 | pPD90_28 | Ampicillin |
| Plate 1 / A / 7 | pPD95_70 | Ampicillin |
| Plate 1 / A / 8 | pPD95_93 | Ampicillin |
| Plate 1 / A / 9 | pPD87_50 | Ampicillin |
| Plate 1 / A / 10 | pPD90_31 | Ampicillin |
| Plate 1 / A / 11 | pPD95_69 | Ampicillin |
| Plate 1 / A / 12 | pPD96_02 | Ampicillin |
| Plate 1 / B / 1 | pPD88_27 | Ampicillin |
| Plate 1 / B / 2 | pPD89_03 | Ampicillin |
| Plate 1 / B / 3 | pPD95_73 | Ampicillin |
| Plate 1 / B / 4 | pPD96_04 | Ampicillin |
| Plate 1 / B / 5 | pPD91_14 | Ampicillin |
| Plate 1 / B / 6 | pPD89_09 | Ampicillin |
| Plate 1 / B / 7 | pPD95_79 | Ampicillin |
| Plate 1 / B / 8 | pOK11_34 | Ampicillin |
| Plate 1 / B / 9 | pPD95_15 | Ampicillin |
| Plate 1 / B / 10 | pPD89_20 | Ampicillin |
| Plate 1 / B / 11 | pPD95_77 | Ampicillin |
| Plate 1 / B / 12 | pPD95_62 | Ampicillin |
| Plate 1 / C / 1 | pPD88_75 | Ampicillin |
| Plate 1 / C / 2 | pPD89_17 | Ampicillin |
| Plate 1 / C / 3 | pPD95_75 | Ampicillin |
| Plate 1 / C / 4 | pPD96_62 | Ampicillin |
| Plate 1 / C / 5 | pPD96_12 | Ampicillin |
| Plate 1 / C / 6 | pPD95_03 | Ampicillin |
| Plate 1 / C / 7 | pPD95_81 | Ampicillin |
| Plate 1 / C / 8 | pPD69_39 | Ampicillin |
| Plate 1 / C / 9 | pPD93_51 | Ampicillin |
| Plate 1 / C / 10 | pPD95_07 | Ampicillin |
| Plate 1 / C / 11 | pPD95_85 | Ampicillin |
| Plate 1 / C / 12 | pPD95_16 | Ampicillin |
| Plate 1 / D / 1 | pPD93_48 | Ampicillin |
| Plate 1 / D / 2 | pPD95_57 | Ampicillin |
| Plate 1 / D / 3 | pPD96_32 | Ampicillin |
| Plate 1 / D / 4 | pPD95_21 | Ampicillin |
| Plate 1 / D / 5 | pPD93_65 | Ampicillin |
| Plate 1 / D / 6 | pPD95_10 | Ampicillin |
| Plate 1 / D / 7 | pPD79_44 | Ampicillin |
| Plate 1 / D / 8 | pPD95_25 | Ampicillin |
| Plate 1 / D / 9 | pPD94_81 | Ampicillin |
| Plate 1 / D / 10 | pPD95_11 | Ampicillin |
| Plate 1 / D / 11 | pPD95_02 | Ampicillin |
| Plate 1 / D / 12 | pPD95_27 | Ampicillin |
| Plate 1 / E / 1 | pPD47_52 | Kanamycin |
| Plate 1 / E / 2 | pPD10_46 | Ampicillin |
| Plate 1 / E / 3 | pPD50_21 | Ampicillin |
| Plate 1 / E / 4 | pICT19h | Ampicillin |
| Plate 1 / E / 5 | pPD57_56 | Ampicillin |
| Plate 1 / E / 6 | pPD26_77 | Ampicillin |
| Plate 1 / E / 7 | pPD20_97 | Ampicillin |
| Plate 1 / E / 8 | pAST_19a | Ampicillin |
| Plate 1 / E / 9 | pPD8_02 | Ampicillin |
| Plate 1 / E / 10 | pPD34_110 | Ampicillin |
| Plate 1 / E / 11 | pPD50_14 | Ampicillin |
| Plate 1 / E / 12 | pAST_18b | Ampicillin |
| Plate 1 / F / 1 | pPD8_33 | Ampicillin |
| Plate 1 / F / 2 | pPD81_34 | Ampicillin |
| Plate 1 / F / 3 | pPD49_78 | Ampicillin |
| Plate 1 / F / 4 | pPD16_43 | Ampicillin |
| Plate 1 / F / 5 | pPD61_125 | Ampicillin |
| Plate 1 / F / 6 | pPD49_83 | Ampicillin |
| Plate 1 / F / 7 | pPD16_51 | Ampicillin |
| Plate 1 / F / 8 | pPD93_97 | Ampicillin |
| Plate 1 / F / 9 | pPD30_69 | Ampicillin |
| Plate 1 / F / 10 | pPD95_18 | Ampicillin |
| Plate 1 / F / 11 | pPD52_37 | Ampicillin |
| Plate 1 / F / 12 | pPD18_32 | Ampicillin |
| Plate 1 / G / 1 | pPD95_86 | Ampicillin |
| Plate 1 / G / 2 | pPD54_01 | Ampicillin |
| Plate 1 / G / 3 | pPD21_28 | Ampicillin |
| Plate 1 / G / 4 | pPD5_41 | Ampicillin |
| Plate 1 / G / 5 | pPD52_102 | Ampicillin |
| Plate 1 / G / 6 | pPD22_11 | Ampicillin |
| Plate 1 / G / 7 | pPD30_35 | Ampicillin |
| Plate 1 / G / 8 | pPD22_04 | Ampicillin |
| Plate 1 / G / 9 | pICT19r | Ampicillin |
| Plate 1 / G / 10 | pPD18_49 | Ampicillin |
| Plate 1 / G / 11 | pPD30_38 | Ampicillin |
| Plate 1 / G / 12 | pDD16_01 | Ampicillin |
| Plate 1 / H / 1 | pPD152.79 | Ampicillin |
| Plate 1 / H / 2 | pPD157.60 | Ampicillin |
| Plate 1 / H / 3 | pPD160.60 | Ampicillin |
| Plate 1 / H / 4 | pPD158.87 | Ampicillin |
| Plate 1 / H / 5 | pPD157.63 | Ampicillin |
| Plate 1 / H / 6 | pPD117.20 | Ampicillin |
| Plate 1 / H / 7 | pPD157.99 | Ampicillin |
| Plate 1 / H / 8 | pPD138.11 | Ampicillin |
| Plate 1 / H / 9 | pPD132.49 | Ampicillin |
| Plate 1 / H / 10 | pPD172.01 | Ampicillin |
| Plate 1 / H / 11 | pPD176.36 | Ampicillin |
| Plate 1 / H / 12 | pPD176.86 | Ampicillin |
| Plate 2 / A / 1 | L2603 | Ampicillin |
| Plate 2 / A / 2 | L3567 | Ampicillin |
| Plate 2 / A / 3 | L2604 | Ampicillin |
| Plate 2 / A / 4 | L3568 | Ampicillin |
| Plate 2 / A / 5 | L2605 | Ampicillin |
| Plate 2 / A / 6 | L3569 | Ampicillin |
| Plate 2 / A / 7 | L2630 | Ampicillin |
| Plate 2 / A / 8 | L3570 | Ampicillin |
| Plate 2 / A / 9 | L2678 | Ampicillin |
| Plate 2 / A / 10 | L3571 | Ampicillin |
| Plate 2 / A / 11 | L2680 | Ampicillin |
| Plate 2 / A / 12 | L3572 | Ampicillin |
| Plate 2 / B / 1 | L3573 | Ampicillin |
| Plate 2 / B / 2 | L2681 | Ampicillin |
| Plate 2 / B / 3 | L3574 | Ampicillin |
| Plate 2 / B / 4 | L2682 | Ampicillin |
| Plate 2 / B / 5 | L3602 | Ampicillin |
| Plate 2 / B / 6 | L2684 | Ampicillin |
| Plate 2 / B / 7 | L3603 | Ampicillin |
| Plate 2 / B / 8 | L2685 | Ampicillin |
| Plate 2 / B / 9 | L3604 | Ampicillin |
| Plate 2 / B / 10 | L2822 | Ampicillin |
| Plate 2 / B / 11 | L3605 | Ampicillin |
| Plate 2 / B / 12 | L2865 | Ampicillin |
| Plate 2 / C / 1 | L2908 | Ampicillin |
| Plate 2 / C / 2 | L3606 | Ampicillin |
| Plate 2 / C / 3 | L2911 | Ampicillin |
| Plate 2 / C / 4 | L3607 | Ampicillin |
| Plate 2 / C / 5 | L2944 | Ampicillin |
| Plate 2 / C / 6 | L3608 | Ampicillin |
| Plate 2 / C / 7 | L2946 | Ampicillin |
| Plate 2 / C / 8 | L3609 | Ampicillin |
| Plate 2 / C / 9 | L2947 | Ampicillin |
| Plate 2 / C / 10 | L3610 | Ampicillin |
| Plate 2 / C / 11 | L2963 | Ampicillin |
| Plate 2 / C / 12 | L3611 | Ampicillin |
| Plate 2 / D / 1 | L3612 | Ampicillin |
| Plate 2 / D / 2 | L3110 | Ampicillin |
| Plate 2 / D / 3 | L3613 | Ampicillin |
| Plate 2 / D / 4 | L3111 | Ampicillin |
| Plate 2 / D / 5 | L3615 | Ampicillin |
| Plate 2 / D / 6 | L3135 | Ampicillin |
| Plate 2 / D / 7 | L3616 | Ampicillin |
| Plate 2 / D / 8 | L3136 | Ampicillin |
| Plate 2 / D / 9 | L3617 | Ampicillin |
| Plate 2 / D / 10 | L3137 | Ampicillin |
| Plate 2 / D / 11 | L3618 | Ampicillin |
| Plate 2 / D / 12 | L3465 | Ampicillin |
| Plate 2 / E / 1 | L3467 | Ampicillin |
| Plate 2 / E / 2 | L3619 | Ampicillin |
| Plate 2 / E / 3 | L3469 | Ampicillin |
| Plate 2 / E / 4 | L3620 | Ampicillin |
| Plate 2 / E / 5 | L3471 | Ampicillin |
| Plate 2 / E / 6 | L3621 | Ampicillin |
| Plate 2 / E / 7 | L3502 | Ampicillin |
| Plate 2 / E / 8 | L3691 | Ampicillin |
| Plate 2 / E / 9 | L3503 | Ampicillin |
| Plate 2 / E / 10 | L3774 | Ampicillin |
| Plate 2 / E / 11 | L3505 | Ampicillin |
| Plate 2 / E / 12 | L3781 | Ampicillin |
| Plate 2 / F / 1 | L3784 | Ampicillin |
| Plate 2 / F / 2 | L3506 | Ampicillin |
| Plate 2 / F / 3 | L3785 | Ampicillin |
| Plate 2 / F / 4 | L3513 | Ampicillin |
| Plate 2 / F / 5 | L3786 | Ampicillin |
| Plate 2 / F / 6 | L3514 | Ampicillin |
| Plate 2 / F / 7 | L3787 | Ampicillin |
| Plate 2 / F / 8 | L3515 | Ampicillin |
| Plate 2 / F / 9 | L3788 | Ampicillin |
| Plate 2 / F / 10 | L3516 | Ampicillin |
| Plate 2 / F / 11 | L3790 | Ampicillin |
| Plate 2 / F / 12 | L3517 | Ampicillin |
| Plate 2 / G / 1 | L3522 | Ampicillin |
| Plate 2 / G / 2 | L3812 | Ampicillin |
| Plate 2 / G / 3 | L3527 | Ampicillin |
| Plate 2 / G / 4 | L3799 | Ampicillin |
| Plate 2 / G / 5 | L3557 | Ampicillin |
| Plate 2 / G / 6 | L3827 | Ampicillin |
| Plate 2 / G / 7 | L3558 | Ampicillin |
| Plate 2 / G / 8 | L3828 | Ampicillin |
| Plate 2 / G / 9 | L3559 | Ampicillin |
| Plate 2 / G / 10 | L3829 | Ampicillin |
| Plate 2 / G / 11 | L3560 | Ampicillin |
| Plate 2 / G / 12 | L3863 | Ampicillin |
| Plate 2 / H / 1 | L3809 | Ampicillin |
| Plate 2 / H / 2 | L3561 | Ampicillin |
| Plate 2 / H / 3 | L3670 | Ampicillin |
| Plate 2 / H / 4 | L3562 | Ampicillin |
| Plate 2 / H / 5 | L3808 | Ampicillin |
| Plate 2 / H / 6 | L3563 | Ampicillin |
| Plate 2 / H / 7 | L3811 | Ampicillin |
| Plate 2 / H / 8 | L3564 | Ampicillin |
| Plate 2 / H / 9 | L3720 | Ampicillin |
| Plate 2 / H / 10 | L3565 | Ampicillin |
| Plate 2 / H / 11 | BK48 | Ampicillin |
| Plate 2 / H / 12 | L3566 | Ampicillin |
| Plate 3 / A / 1 | L4816 | Ampicillin |
| Plate 3 / A / 2 | L4053 | Ampicillin |
| Plate 3 / A / 3 | L4663 | Ampicillin |
| Plate 3 / A / 4 | L4051 | Ampicillin |
| Plate 3 / A / 5 | L4686 | Ampicillin |
| Plate 3 / A / 6 | L4040 | Ampicillin |
| Plate 3 / A / 7 | L4665 | Ampicillin |
| Plate 3 / A / 8 | L4037 | Ampicillin |
| Plate 3 / A / 9 | L4664 | Ampicillin |
| Plate 3 / A / 10 | L4042 | Ampicillin |
| Plate 3 / A / 11 | L4682 | Ampicillin |
| Plate 3 / A / 12 | L4043 | Ampicillin |
| Plate 3 / B / 1 | L4038 | Ampicillin |
| Plate 3 / B / 2 | L4660 | Ampicillin |
| Plate 3 / B / 3 | L4453 | Ampicillin |
| Plate 3 / B / 4 | L4780 | Ampicillin |
| Plate 3 / B / 5 | L4455 | Ampicillin |
| Plate 3 / B / 6 | L4752 | Ampicillin |
| Plate 3 / B / 7 | L4759 | Ampicillin |
| Plate 3 / B / 8 | L4817 | Ampicillin |
| Plate 3 / B / 9 | L4796 | Ampicillin |
| Plate 3 / B / 10 | L4643 | Ampicillin |
| Plate 3 / B / 11 | L4809 | Ampicillin |
| Plate 3 / B / 12 | L4687 | Ampicillin |
| Plate 3 / C / 1 | L4667 | Ampicillin |
| Plate 3 / C / 2 | L4057 | Ampicillin |
| Plate 3 / C / 3 | L4666 | Ampicillin |
| Plate 3 / C / 4 | L4058 | Ampicillin |
| Plate 3 / C / 5 | L4683 | Ampicillin |
| Plate 3 / C / 6 | L4035 | Ampicillin |
| Plate 3 / C / 7 | L4671 | Ampicillin |
| Plate 3 / C / 8 | L453 | Ampicillin |
| Plate 3 / C / 9 | L4781 | Ampicillin |
| Plate 3 / C / 10 | L4129 | Ampicillin |
| Plate 3 / C / 11 | L4753 | Ampicillin |
| Plate 3 / C / 12 | L4119 | Ampicillin |
| Plate 3 / D / 1 | L4138 | Ampicillin |
| Plate 3 / D / 2 | L4640 | Ampicillin |
| Plate 3 / D / 3 | L4819 | Ampicillin |
| Plate 3 / D / 4 | L4061 | Ampicillin |
| Plate 3 / D / 5 | L4440 | Ampicillin |
| Plate 3 / D / 6 | L4062 | Ampicillin |
| Plate 3 / D / 7 | pLT61 | Ampicillin |
| Plate 3 / D / 8 | L4063 | Ampicillin |
| Plate 3 / D / 9 | pLT63 | Ampicillin |
| Plate 3 / D / 10 | L4064 | Ampicillin |
| Plate 3 / D / 11 | L4417 | Ampicillin |
| Plate 3 / D / 12 | L4065 | Ampicillin |
| Plate 3 / E / 1 | L4066 | Ampicillin |
| Plate 3 / E / 2 | L4400 | Ampicillin |
| Plate 3 / E / 3 | L4095 | Ampicillin |
| Plate 3 / E / 4 | L4271 | Ampicillin |
| Plate 3 / E / 5 | L4096 | Ampicillin |
| Plate 3 / E / 6 | L4404 | Ampicillin |
| Plate 3 / E / 7 | L4458 | Ampicillin |
| Plate 3 / E / 8 | pPD191.45 | Ampicillin |
| Plate 3 / E / 9 | L4506 | Ampicillin |
| Plate 3 / E / 10 | L4431 | Ampicillin |
| Plate 3 / E / 11 | L4507 | Ampicillin |
| Plate 3 / E / 12 | L4842 | Ampicillin |
| Plate 3 / F / 1 | L4437 | Ampicillin |
| Plate 3 / F / 2 | L4632 | Ampicillin |
| Plate 3 / F / 3 | L4438 | Ampicillin |
| Plate 3 / F / 4 | L4054 | Ampicillin |
| Plate 3 / F / 5 | pBK125 | Ampicillin |
| Plate 3 / F / 6 | L4052 | Ampicillin |
| Plate 3 / F / 7 | pPD187.49 | Ampicillin |
| Plate 3 / F / 8 | L4019 | Ampicillin |
| Plate 3 / F / 9 | L754 | Ampicillin |
| Plate 3 / F / 10 | L4047 | Ampicillin |
| Plate 3 / F / 11 | L2528 | Ampicillin |
| Plate 3 / F / 12 | L4018 | Ampicillin |
| Plate 3 / G / 1 | L4046 | Ampicillin |
| Plate 3 / G / 2 | L2534 | Ampicillin |
| Plate 3 / G / 3 | L4020 | Ampicillin |
| Plate 3 / G / 4 | L2531 | Ampicillin |
| Plate 3 / G / 5 | L4048 | Ampicillin |
| Plate 3 / G / 6 | L3929 | Ampicillin |
| Plate 3 / G / 7 | L4041 | Ampicillin |
| Plate 3 / G / 8 | L3928 | Ampicillin |
| Plate 3 / G / 9 | L4026 | Ampicillin |
| Plate 3 / G / 10 | L3930 | Ampicillin |
| Plate 3 / G / 11 | L4049 | Ampicillin |
| Plate 3 / G / 12 | L3920 | Ampicillin |
| Plate 3 / H / 1 | L3927 | Ampicillin |
| Plate 3 / H / 2 | pJKL464 | Ampicillin |
| Plate 3 / H / 3 | pPD177.53 | Ampicillin |
| Plate 3 / H / 4 | pPD177.62 | Ampicillin |
| Plate 3 / H / 5 | pPD177.66 | Ampicillin |
| Plate 3 / H / 6 | pPD177.71 | Ampicillin |
| Plate 3 / H / 7 | pPD187.24 | Ampicillin |
| Plate 3 / H / 8 | pPD159.88 | Ampicillin |
| Plate 3 / H / 9 | pPD107.09 | Ampicillin |
| Plate 3 / H / 10 | pPD158.114 | Ampicillin |
| Plate 3 / H / 11 | pPD158.117 | Ampicillin |
| Plate 3 / H / 12 | pPD159.62 | Ampicillin |