Yeast GPCR-sensor Toolkit
(Kit #
1000000157
)
Depositing Lab: Tom Ellis
The Yeast GPCR-sensor Toolkit includes 42 plasmids for constructing highly-tunable GPCR-based biosensors in yeast.
This kit will be sent as bacterial glycerol stocks in 96-well plate format.
Original Publication
Engineering a Model Cell for Rational Tuning of GPCR Signaling. Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Öling D, Larsson N, Wigglesworth M, Ladds G, Ellis T. Cell. 2019 Apr 18;177(3):782-796.e27. doi: 10.1016/j.cell.2019.02.023. PubMed PMID: 30955892.
Description
The Ellis Lab Yeast GPCR-sensor Toolkit consists of 42 parts for building highly-tunable GPCR-based biosensors in yeast. The parts are formatted for use with, and require, MoClo-YTK (Kit #1000000061) from the Dueber Lab (Lee et al., 2015), and are designed to work with the engineered yeast strain yWS677 (soon available from ATCC).
Note from the depositor: Part pWS932 contains a 50/50 ratio of C/A at position 662, which could lead to the substitution of a serine to a tyrosine (S235Y). We recommend sequencing assembled plasmids using this part to confirm the presence of the correct base pair at this position.
Ordering Note: If you would like to order both the Yeast GPCR-sensor Toolkit and MoClo-YTK (Kit #1000000061), or have previously ordered MoClo-YTK (Kit #1000000061) and would now like to order the Yeast GPCR-sensor Toolkit, please email [email protected] for pricing (if you use a distributor in your country, please contact your distributor directly for pricing).
Art by Sigrid Knemeyer, SciStories LLC, scistories.com.
How to Cite this Kit
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.
For your Materials and Methods section:
“The Yeast GPCR-sensor Toolkit was a gift from Tom Ellis (Addgene kit #1000000157).”
For your Reference section:
Engineering a Model Cell for Rational Tuning of GPCR Signaling. Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Öling D, Larsson N, Wigglesworth M, Ladds G, Ellis T. Cell. 2019 Apr 18;177(3):782-796.e27. doi: 10.1016/j.cell.2019.02.023. PubMed PMID: 30955892.
Yeast GPCR-sensor Toolkit - #1000000157
- Resistance Color Key
Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.
- Inventory
Searchable and sortable table of all plasmids in kit. The Well column lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.
- Kit Plate Map
96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.
Resistance Color Key
| Chloramphenicol |
Inventory
| Well | Plasmid | Resistance |
|---|---|---|
| A / 1 | pWS060 | Chloramphenicol |
| A / 2 | pWS053 | Chloramphenicol |
| A / 3 | pWS2247 | Chloramphenicol |
| A / 4 | pWS1078 | Chloramphenicol |
| A / 5 | pWS480 | Chloramphenicol |
| A / 6 | pWS481 | Chloramphenicol |
| A / 7 | pWS482 | Chloramphenicol |
| A / 8 | pWS483 | Chloramphenicol |
| A / 9 | pWS484 | Chloramphenicol |
| A / 10 | pWS485 | Chloramphenicol |
| A / 11 | pWS494 | Chloramphenicol |
| A / 12 | pWS491 | Chloramphenicol |
| B / 1 | pWS487 | Chloramphenicol |
| B / 2 | pWS493 | Chloramphenicol |
| B / 3 | pRC246 | Chloramphenicol |
| B / 4 | pWS486 | Chloramphenicol |
| B / 5 | pWS498 | Chloramphenicol |
| B / 6 | pWS496 | Chloramphenicol |
| B / 7 | pWS497 | Chloramphenicol |
| B / 8 | pWS495 | Chloramphenicol |
| B / 9 | pWS067 | Chloramphenicol |
| B / 10 | pWS626 | Chloramphenicol |
| B / 11 | pWS625 | Chloramphenicol |
| B / 12 | pWS1806 | Chloramphenicol |
| C / 1 | pWS2119 | Chloramphenicol |
| C / 2 | pWS1037 | Chloramphenicol |
| C / 3 | pWS936 | Chloramphenicol |
| C / 4 | pWS939 | Chloramphenicol |
| C / 5 | pWS926 | Chloramphenicol |
| C / 6 | pWS931 | Chloramphenicol |
| C / 7 | pWS930 | Chloramphenicol |
| C / 8 | pWS932 | Chloramphenicol |
| C / 9 | pWS033 | Chloramphenicol |
| C / 10 | pRC063 | Chloramphenicol |
| C / 11 | pWS1837 | Chloramphenicol |
| C / 12 | pWS788 | Chloramphenicol |
| D / 1 | pWS081 | Chloramphenicol |
| D / 2 | pWS509 | Chloramphenicol |
| D / 3 | pWS510 | Chloramphenicol |
| D / 4 | pWS511 | Chloramphenicol |
| D / 5 | pWS627 | Chloramphenicol |
| D / 6 | pWS1859 | Chloramphenicol |