Multiplex Yeast Toolkit (MYT)
(Kit #
1000000229
)
Depositing Lab: Tom Ellis
The Multiplex Yeast Toolkit (MYT) is a collection of 96 plasmids which extend the capabilities of the MoClo Yeast Toolkit (YTK, Addgene Kit #1000000061) for genetically engineering the yeast, Saccharomyces cerevisiae.
This kit will be sent as bacterial glycerol stocks in 96-well plate format.
Original Publication
A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of Saccharomyces cerevisiae. Shaw WM, Khalil AS, Ellis T. ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423. PubMed (Link opens in a new window) Article (Link opens in a new window).
Description
MYT includes a set of new integration vectors and selectable markers usable across common lab strains, as well as additional assembly cassettes to increase the number of transcription units in multigene constructs, CRISPR-Cas9 tools for highly efficient multiplexed editing, and three orthogonal and inducible promoter systems for conditional programming of gene expression. MYT is fully backward compatible with YTK, using the same bottom-up hierarchical assembly of single and multi-gene constructs, based on the Golden Gate cloning technique. Integration vectors and assembly cassettes are pre-assembled and ready to go, straight out of the kit, for convenience. Additionally, all of the alternative cloning methods included in YTK, such as BioBrick compatibility, are also present in MYT, while simplifying yeast in vivo assembly.
Figure 1: Overview of constructs found in the MYT including plasmids for genome integration, multiplex CRISPR-Cas9, transcriptional units, and conditional gene expression.
How to Cite this Kit
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.
For your Materials and Methods section:
“The Multiplex Yeast Toolkit (MYT) was a gift from Tom Ellis (Addgene kit #1000000229).”
For your Reference section:
A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of Saccharomyces cerevisiae. Shaw WM, Khalil AS, Ellis T. ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423. PubMed (Link opens in a new window) Article (Link opens in a new window).
Multiplex Yeast Toolkit (MYT) - #1000000229
- Resistance Color Key
Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.
- Inventory
Searchable and sortable table of all plasmids in kit. The Well column lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.
- Kit Plate Map
96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.
Resistance Color Key
| Chloramphenicol | |
| Ampicillin | |
| Kanamycin | |
| Spectinomycin |
Inventory
| Well | Plasmid | Resistance |
|---|---|---|
| A / 1 | pMYT001 | Chloramphenicol |
| A / 2 | pMYT002 | Chloramphenicol |
| A / 3 | pMYT003 | Chloramphenicol |
| A / 4 | pMYT004 | Chloramphenicol |
| A / 5 | pMYT005 | Chloramphenicol |
| A / 6 | pMYT006 | Chloramphenicol |
| A / 7 | pMYT007 | Chloramphenicol |
| A / 8 | pMYT008 | Chloramphenicol |
| A / 9 | pMYT009 | Chloramphenicol |
| A / 10 | pMYT010 | Chloramphenicol |
| A / 11 | pMYT011 | Chloramphenicol |
| A / 12 | pMYT012 | Chloramphenicol |
| B / 1 | pMYT013 | Chloramphenicol |
| B / 2 | pMYT014 | Chloramphenicol |
| B / 3 | pMYT015 | Chloramphenicol |
| B / 4 | pMYT016 | Chloramphenicol |
| B / 5 | pMYT017 | Chloramphenicol |
| B / 6 | pMYT018 | Chloramphenicol |
| B / 7 | pMYT019 | Chloramphenicol |
| B / 8 | pMYT020 | Chloramphenicol |
| B / 9 | pMYT021 | Chloramphenicol |
| B / 10 | pMYT022 | Chloramphenicol |
| B / 11 | pMYT023 | Chloramphenicol |
| B / 12 | pMYT024 | Chloramphenicol |
| C / 1 | pMYT025 | Chloramphenicol |
| C / 2 | pMYT026 | Chloramphenicol |
| C / 3 | pMYT027 | Chloramphenicol |
| C / 4 | pMYT028 | Chloramphenicol |
| C / 5 | pMYT029 | Chloramphenicol |
| C / 6 | pMYT030 | Chloramphenicol |
| C / 7 | pMYT031 | Chloramphenicol |
| C / 8 | pMYT032 | Chloramphenicol |
| C / 9 | pMYT033 | Chloramphenicol |
| C / 10 | pMYT034 | Chloramphenicol |
| C / 11 | pMYT035 | Chloramphenicol |
| C / 12 | pMYT036 | Chloramphenicol |
| D / 1 | pMYT037 | Chloramphenicol |
| D / 2 | pMYT038 | Chloramphenicol |
| D / 3 | pMYT039 | Ampicillin |
| D / 4 | pMYT040 | Ampicillin |
| D / 5 | pMYT041 | Ampicillin |
| D / 6 | pMYT042 | Ampicillin |
| D / 7 | pMYT043 | Ampicillin |
| D / 8 | pMYT044 | Ampicillin |
| D / 9 | pMYT045 | Ampicillin |
| D / 10 | pMYT046 | Ampicillin |
| D / 11 | pMYT047 | Ampicillin |
| D / 12 | pMYT048 | Ampicillin |
| E / 1 | pMYT049 | Ampicillin |
| E / 2 | pMYT050 | Ampicillin |
| E / 3 | pMYT051 | Ampicillin |
| E / 4 | pMYT052 | Ampicillin |
| E / 5 | pMYT053 | Ampicillin |
| E / 6 | pMYT054 | Ampicillin |
| E / 7 | pMYT055 | Ampicillin |
| E / 8 | pMYT056 | Ampicillin |
| E / 9 | pMYT057 | Ampicillin |
| E / 10 | pMYT058 | Ampicillin |
| E / 11 | pMYT059 | Ampicillin |
| E / 12 | pMYT060 | Ampicillin |
| F / 1 | pMYT061 | Ampicillin |
| F / 2 | pMYT062 | Ampicillin |
| F / 3 | pMYT063 | Ampicillin |
| F / 4 | pMYT064 | Ampicillin |
| F / 5 | pMYT065 | Ampicillin |
| F / 6 | pMYT066 | Ampicillin |
| F / 7 | pMYT067 | Ampicillin |
| F / 8 | pMYT068 | Ampicillin |
| F / 9 | pMYT069 | Ampicillin |
| F / 10 | pMYT070 | Ampicillin |
| F / 11 | pMYT071 | Ampicillin |
| F / 12 | pMYT072 | Ampicillin |
| G / 1 | pMYT073 | Ampicillin |
| G / 2 | pMYT074 | Ampicillin |
| G / 3 | pMYT075 | Kanamycin |
| G / 4 | pMYT076 | Kanamycin |
| G / 5 | pMYT077 | Kanamycin |
| G / 6 | pMYT078 | Kanamycin |
| G / 7 | pMYT079 | Kanamycin |
| G / 8 | pMYT080 | Kanamycin |
| G / 9 | pMYT081 | Kanamycin |
| G / 10 | pMYT082 | Kanamycin |
| G / 11 | pMYT083 | Kanamycin |
| G / 12 | pMYT084 | Kanamycin |
| H / 1 | pMYT085 | Kanamycin |
| H / 2 | pMYT086 | Kanamycin |
| H / 3 | pMYT087 | Kanamycin |
| H / 4 | pMYT088 | Kanamycin |
| H / 5 | pMYT089 | Kanamycin |
| H / 6 | pMYT090 | Kanamycin |
| H / 7 | pMYT091 | Kanamycin |
| H / 8 | pMYT092 | Kanamycin |
| H / 9 | pMYT093 | Kanamycin |
| H / 10 | pMYT094 | Kanamycin |
| H / 11 | pMYT095 | Spectinomycin |
| H / 12 | pMYT096 | Spectinomycin |