EXPRESSYALI
(Kit #
1000000245
)
Depositing Lab: Irina Borodina
The EXPRESSYALI Set is a complete kit for the introduction of genes into Yarrowia chromosomal loci. The kit includes six part plasmids, 12 marker-free integrative vectors, and 11 gRNA plasmids.
This kit will be sent as bacterial glycerol stocks in 96-well plate format.
Original Publication
Combinatorial iterative method for metabolic engineering of Yarrowia lipolytica: application for betanin biosynthesis. Jiang W, Wang S, Avila P, Jorgensen TS, Yang Z, Borodina I. Metab Eng. 2024 Sep 9:S1096-7176(24)00110-1. doi: 10.1016/j.ymben.2024.09.003. PubMed (Link opens in a new window) Article (Link opens in a new window).
Description
The EXPRESSYALI collection facilitates the hierarchical assembly of single and multi-gene constructs, up to three transcription units, for Yarrowia lipolytica expression. Developed by Irina Borodina’s lab, this toolkit includes three well-characterized promoters, three terminators, 12 marker-free integrative vectors, and 11 gRNA plasmids. It employs the Golden Gate cloning method to enable the generation of a combinatorial library from three distinct transcription units. Additionally, the toolkit utilizes two interchangeable selection markers—NatMX for nourseothricin and HphMX for hygromycin—in successive transformation rounds, eliminating the need for marker excision.
- Figure 1. EXPRESSYALI system workflow consisted of iterative rounds of transformation of combinatorial libraries into yeast Y. lipolytica with selection using high-throughput technology. HR: homologous arm. Ori: the origin of replication. SP: spectinomycin. AMP: ampicillin.
How to Cite this Kit
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.
For your Materials and Methods section:
“The EXPRESSYALI was a gift from Irina Borodina (Addgene kit #1000000245).”
For your Reference section:
Combinatorial iterative method for metabolic engineering of Yarrowia lipolytica: application for betanin biosynthesis. Jiang W, Wang S, Avila P, Jorgensen TS, Yang Z, Borodina I. Metab Eng. 2024 Sep 9:S1096-7176(24)00110-1. doi: 10.1016/j.ymben.2024.09.003. PubMed (Link opens in a new window) Article (Link opens in a new window).
EXPRESSYALI - #1000000245
- Resistance Color Key
Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.
- Inventory
Searchable and sortable table of all plasmids in kit. The Well column lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.
- Kit Plate Map
96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.
Resistance Color Key
| Ampicillin | |
| Spectinomycin | |
| Kanamycin | |
| Chloramphenicol |
Inventory
| Well | Plasmid | Resistance |
|---|---|---|
| A / 1 | pCfB12714 | Ampicillin |
| A / 2 | pCfB12715 | Ampicillin |
| A / 3 | pCfB12716 | Ampicillin |
| A / 4 | pCfB12717 | Ampicillin |
| A / 5 | pCfB12718 | Ampicillin |
| A / 6 | pCfB13017 | Ampicillin |
| A / 7 | pCfB10769 | Ampicillin |
| A / 8 | pCfB11787 | Ampicillin |
| A / 9 | pCfB6637 | Ampicillin |
| A / 10 | pCfB8860 | Ampicillin |
| A / 11 | pCfB6638 | Ampicillin |
| A / 12 | pCfB11785 | Ampicillin |
| B / 1 | pCfB6630 | Ampicillin |
| B / 2 | pCfB8856 | Ampicillin |
| B / 3 | pCfB12719 | Ampicillin |
| B / 4 | pCfB8866 | Ampicillin |
| B / 5 | pCfB12720 | Ampicillin |
| B / 6 | pCfB12711 | Spectinomycin |
| B / 7 | pCfB12712 | Spectinomycin |
| B / 8 | pCfB12713 | Spectinomycin |
| B / 9 | pCfB13014 | Spectinomycin |
| B / 10 | pCfB13015 | Spectinomycin |
| B / 11 | pCfB13016 | Spectinomycin |
| B / 12 | pCfB12706 | Kanamycin |
| C / 1 | pCfB12708 | Kanamycin |
| C / 2 | pCfB12710 | Kanamycin |
| C / 3 | PCfB12709 | Kanamycin |
| C / 4 | pCfB13089 | Chloramphenicol |
| C / 5 | pCfB13090 | Chloramphenicol |