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Keith Joung Lab Materials

Keith Joung Lab Materials

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Showing 1 to 10 of 35 entries

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Title	Authors	Publication
A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing.	Grunewald J, Zhou R, Lareau CA, Garcia SP, Iyer S, Miller BR, Langner LM, Hsu JY, Aryee MJ, Joung JK	Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0535-y. doi: 10.1038/s41587-020-0535-y.
An improved zinc-finger nuclease architecture for highly specific genome editing.	Miller JC, Holmes MC, Wang J, Guschin DY, Lee YL, Rupniewski I, Beausejour CM, Waite AJ, Wang NS, Kim KA, Gregory PD, Pabo CO, Rebar EJ	Nat Biotechnol. 2007 Jul . 25(7):778-85.
Augmenting and directing long-range CRISPR-mediated activation in human cells.	Tak YE, Horng JE, Perry NT, Schultz HT, Iyer S, Yao Q, Zou LS, Aryee MJ, Pinello L, Joung JK	Nat Methods. 2021 Sep;18(9):1075-1081. doi: 10.1038/s41592-021-01224-1. Epub 2021 Aug 5.
Autonomous zinc-finger nuclease pairs for targeted chromosomal deletion.	Soellu C, Pars K, Cornu TI, Thibodeau-Beganny S, Maeder ML, Joung JK, Heilbronn R, Cathomen T	Nucleic Acids Res. 2010 Dec 1. 38(22):8269-76.
Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition.	Kleinstiver BP, Prew MS, Tsai SQ, Nguyen NT, Topkar VV, Zheng Z, Joung JK	Nat Biotechnol. 2015 Nov 2. doi: 10.1038/nbt.3404.
CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells.	Kurt IC, Zhou R, Iyer S, Garcia SP, Miller BR, Langner LM, Grunewald J, Joung JK	Nat Biotechnol. 2020 Jul 20. pii: 10.1038/s41587-020-0609-x. doi: 10.1038/s41587-020-0609-x.
CRISPR DNA base editors with reduced RNA off-target and self-editing activities.	Grunewald J, Zhou R, Iyer S, Lareau CA, Garcia SP, Aryee MJ, Joung JK	Nat Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 10.1038/s41587-019-0236-6.
CRISPR prime editing with ribonucleoprotein complexes in zebrafish and primary human cells.	Petri K, Zhang W, Ma J, Schmidts A, Lee H, Horng JE, Kim DY, Kurt IC, Clement K, Hsu JY, Pinello L, Maus MV, Joung JK, Yeh JJ	Nat Biotechnol. 2021 Apr 29. pii: 10.1038/s41587-021-00901-y. doi: 10.1038/s41587-021-00901-y.
CRISPR RNA-guided activation of endogenous human genes.	Maeder ML, Linder SJ, Cascio VM, Fu Y, Ho QH, Joung JK	Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2598.
Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing.	Tsai SQ, Wyvekens N, Khayter C, Foden JA, Thapar V, Reyon D, Goodwin MJ, Aryee MJ, Joung JK	Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908.

Showing 1 to 10 of 1,363 entries

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	ID	Plasmid	Gene/Insert	Vector Type	Publication
100.401.364811.47511	47511	pFYF1320 EGFP Site#1	gRNA-EGFP site 1	Mammalian Expression, CRISPR	High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nat Biotechnol. 2013 Jun 23. doi: 10.1038/nbt.2623.
100.115.175360.43860	43860	MLM3636	None	Mammalian Expression, CRISPR	Joung lab unpublished CRISPR plasmids (unpublished)
100.52.177987.101178	101178	BPK4410 - human expression plasmid for SpCas9 Cluster 1 (HypaCas9)	hSpCas9-Cluster1(N692A/M694A/Q695A/H698A)	Mammalian Expression	Enhanced proofreading governs CRISPR-Cas9 targeting accuracy. Nature. 2017 Sep 20. doi: 10.1038/nature24268.
100.35.794343.65777	65777	BPK1520	SpCas9 gRNA backbone, without spacer sequence	Mammalian Expression, CRISPR	Engineered CRISPR-Cas9 nucleases with altered PAM specificities. Nature. 2015 Jun 22. doi: 10.1038/nature14592.
100.32.345996.65767	65767	BPK764	mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA	Bacterial Expression, CRISPR	Engineered CRISPR-Cas9 nucleases with altered PAM specificities. Nature. 2015 Jun 22. doi: 10.1038/nature14592.
100.17.682018.13422	13422	pBAC-lacZ		Bacterial Expression, Cre/Lox	Standardized reagents and protocols for engineering zinc finger nucleases by modular assembly. Nat Protoc. 2006;1(3):1637-52. doi: 10.1038/nprot.2006.259
100.7.462612.42250	42250	DR274		Worm Expression, CRISPR	Efficient genome editing in zebrafish using a CRISPR-Cas system. Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2501.
100.6.144325.70709	70709	BPK2660		Mammalian Expression, CRISPR	Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition. Nat Biotechnol. 2015 Nov 2. doi: 10.1038/nbt.3404.
100.6.055614.107941	107941	pCAG-enAsCas12a(E174R/S542R/K548R)-NLS(nuc)-3xHA (AAS848)	human codon optimized enAsCas12a (E174R/S542R/K548R)	Mammalian Expression	Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0.
100.5.826533.72247	72247	VP12	mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag	Mammalian Expression, CRISPR	High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects. Nature. 2016 Jan 6. doi: 10.1038/nature16526.

Showing 1 to 3 of 3 entries

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ID	Pooled Library	Vector Type	Publication
160132	p11-3’_8xN_PAM-site1 (RTW554)	CRISPR	Scalable characterization of the PAM requirements of CRISPR-Cas enzymes using HT-PAMDA. Nat Protoc. 2021 Feb 5. pii: 10.1038/s41596-020-00465-2. doi: 10.1038/s41596-020-00465-2.
160133	p11-3’_8xN_PAM-site2 (RTW555)	CRISPR	Scalable characterization of the PAM requirements of CRISPR-Cas enzymes using HT-PAMDA. Nat Protoc. 2021 Feb 5. pii: 10.1038/s41596-020-00465-2. doi: 10.1038/s41596-020-00465-2.
160135	p11-5’_10xN_PAM-site4 (RTW574)	CRISPR	Scalable characterization of the PAM requirements of CRISPR-Cas enzymes using HT-PAMDA. Nat Protoc. 2021 Feb 5. pii: 10.1038/s41596-020-00465-2. doi: 10.1038/s41596-020-00465-2.

Showing 1 to 4 of 4 entries

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	ID	Kit
100.0.743104.1000000017	1000000017	Joung Lab REAL Assembly TALEN Kit
100.0.540246.1000000005	1000000005	Zinc Finger Consortium Expression Vector and Modular Assembly Kit
100.0.450363.1000000010	1000000010	Zinc Finger Consortium Expression Vector Kit v1.0
50.0.284497.1000000013	1000000013	Zinc Finger Consortium OPEN Accessory Reagent Set

Showing 1 to 3 of 3 entries

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	ID	Bacterial Strain
100.0.846340.13424	13424	KJBAC1 strain
100.0.312852.21874	21874	Strain KJ1C
100.0.285560.21875	21875	Strain CSH100

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