Fluorescent Proteins: Plasmids and Resources

Addgene's plasmid repository contains thousands of fluorescent proteins. Use this guide to learn more about the many applications of fluorescent proteins and to find plasmids for your next experiment.

Browse Fluorescent Protein Plasmids by Category

Empty Backbones

Understand the localization and/or function of your protein of interest by fusing it to a fluorescent protein.

FRET

Find FRET pairs and standards to study protein-protein interactions or conformational changes within a protein.

Biosensors

Monitor small biomolecules or other physiological intracellular processes with genetically encoded fluorescent biosensors.

Optogenetics Guide | Optogenetics Plasmids

Use light to activate, inhibit, and control molecular signals, cells, or groups of cells.

Chemogenetics Guide | Chemogenetics Plasmids

Use small molecules to activate or inhibit genetically engineered cellular receptors that affect signalling pathways within cells.

Subcellular Localization

Determine where your protein of interest resides by using a well-characterized fluorescent fusion protein.

In Vivo Imaging

Image with far-red or infrared tools to study molecular processes in organs and whole mammals.

Michael Davidson Fluorescent Protein Collection

Browse the comprehensive Michael Davidson collection consisting of ORFs tagged with a variety of fluorescent proteins, as well as over 100 empty backbones.

Additional Resources at Addgene

• Addgene Blog: Fluorescent Proteins
• Luciferase Plasmid Collection
• Fluorescent Protein Kits
• Allen Institute for Cell Science Plasmid Collection
• New England BioLabs Cell-Imaging Plasmid Collection
• Promega Plasmid Collection
• Botman-Teusink Yeast Fluorescent Protein Collection

External Resources

• FPbase: an open-source, community-editable database for fluorescent proteins created by Talley Lambert
• Nikon MicroscopyU
• Olympus Microscopy Resource Center
• Leica Microsystems Resources for Microscopy in Life Sciences