Basic AVEXIS Plasmids
AVEXIS is an avidity-based extracellular interaction screen to detect protein interactions, where the extracellular regions of receptor proteins are expressed as both monomeric baits and multimerised enzyme-tagged preys.
The Basic AVEXIS palsmid set is all that you will need to start producing proteins for AVEXIS screening. It includes negative control bait and preys (containing the Cd4 tag alone) and the CD200 and CD200 receptor bait and prey plasmids as a positive control. Please see the following references for more information:
- Large-scale screening for novel low-affinity extracellular protein interactions. Bushell KM et al., Genome Res 2008 Apr;18(4):622-30. PubMed 18296487
- A benchmarked protein microarray-based platform for the identification of novel low-affinity extracellular protein interactions Sun Y et al., Analytical Biochem 2012 May 1;424(1):45-53. PubMed 22342946
| Plasmid ID | Plasmid Name |
|---|---|
| 32402 | Cd4d3+4-bio |
| 32403 | CD4d3+4-blac |
| 32404 | CD200Cd4d3+4-bio |
| 32405 | CD200Cd4d3+4-blac |
| 32406 | CD200RCd4d3+4-bio |
| 32407 | CD200RCd4d3+4-blac |
| 32408 | secretedBirA-8his |
| 64395 | secreted BirA-Flag |