Feng Zhang - Multiplexed Overexpression of Regulatory Factors (MORF) Collection
The MORF library is a comprehensive human transcription factor collection that contains 1,836 genes encoded by 3,548 isoforms and two control vectors. The individual clones, or the pooled library containing the entire pooled collection, can be purchased at Addgene. Each transcription factor isoform has a unique 24 bp barcode that simplifies identification of the transcription factor isoform in pooled screens. This collection and library can be used for screening transcription factors for applications such as cellular programming or cancer drug resistance.
Protocols & References
A transcription factor atlas of directed differentiation. Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F. Cell 2023 Jan 5; 186(1):209-229. doi: 10.1016/j.cell.2022.11.026.
- TF ORF barcode PCR for NGS QC and bulk screening (85 KB)
- TF ORF barcode dial out PCR for 10X scRNA-seq (59.2 KB)
Plasmids
| ID | Plasmid | Gene/Insert |
|---|
Pooled Library
The Multiplexed Overexpression of Regulatory Factors (MORF) pooled library is a comprehensive collection of human transcription factor open reading frames. Each isoform is uniquely barcoded with a 24 bp sequence, to simplify identification during screening.
| Library | ID | PI | Description |
|---|---|---|---|
| 137000, 192821, 1000000218 | A collection of human transcription factor open reading frames with or without GFP and mCherry controls. |
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