Ginkgo Bioworks COVID-19 Plasmid Collection
Researchers developing novel diagnostics, therapeutics, and vaccines against COVID-19 all rely on DNA encoding viral proteins as a first step for many different kinds of important experiments. In an effort to speed up research of SARS-CoV-2, Ginkgo Bioworks (Link opens in a new window) used their DNA design and synthesis capabilities, in collaboration with researchers at Stanford University’s Department of Bioengineering, to build DNA that will enable researchers to express SARS-CoV-2 viral proteins in several expression systems.
Addgene has partnered with Ginkgo to make these plasmids available to scientists in academic and industrial organizations. For more information on ordering from a for-profit entity, please see our Plasmid Distribution to Industry page.
These plasmids have not been expressed or functionally validated by Ginkgo or Addgene.
Plasmids
Use the search bar or sort buttons to find SARS-CoV-2 and common coronvirus plasmids based on:
- Gene insert (e.g. spike, envelope, nsp3, OC43, NL63, HKU1, and 229E)
- Expression type (e.g. bacterial, yeast, or mammalian)
- Tags (e.g. 6xHis, Flag, StrepII, HA, Myc, SUMO, GST, MBP, CBP, S-tag, TAP tag, TRX, or V5)
- Mutations
- Wild type (wt)
- Codon-optimized expression (recode)
- Truncated for making pseudovirus (Ctrunc)
| ID | Plasmid | Description | Mutations | Tags | Industry |
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Additional Resources
- Read Addgene's Blog Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems for a summary of the Ginkgo collection at Addgene.
- Addgene's COVID-19 and Coronavirus Plasmids & Resources page outlines our entire COVID-19 collection. Additionally, we have linked to collections of open-access articles, protocols, and other resource collections related to COVID-19 that may be of use to scientists.
- Addgene's Protocol for RNA Extraction Without A Kit