Allen Institute for Cell Science Plasmid Collection
The Allen Institute for Cell Science has produced the first publicly available collection (Link opens in a new window) of fluorescently tagged, human, induced pluripotent stem cell (hiPSC) lines. The plasmids used to generate these lines have been deposited here with Addgene for distribution to the research community.
With these plasmids researchers will be able to generate fluorescently tagged proteins of key cellular structures in human cell lines of their choice. For a full description of how to use these plasmids, check out Plasmids for Endogenous Gene Tagging in Human Cells on the Addgene Blog.
Plasmids for Endogenous Gene Tagging in Human Cells
Find additional cell line information from the Allen Institute for Cell Science data portal (Link opens in a new window), including:
- data and images from the hiPS cell lines
- gRNA sequences used in the creation of the hiPS cell lines
The following plasmids can be used to create cell lines with endogenously-tagged cellular structures.
| Addgene ID | Plasmid | Allen Institute ID | Tag | Protein | Structure |
|---|---|---|---|---|---|
| 87420 | PXN-EGFP | AICSDP-1 | EGFP | Paxillin | Matrix Adhesions |
| 87421 | TUBA1B-mEGFP | AICSDP-4 | mEGFP | Alpha-tubulin | Microtubules |
| 87422 | LMNB1-mEGFP | AICSDP-10 | mEGFP | Lamin B1 | Nuclear envelope |
| 87423 | TOMM20-mEGFP | AICSDP-8 | mEGFP | Tom20 | Mitochondria |
| 87424 | DSP-mEGFP | AICSDP-9 | mEGFP | Desmoplakin | Desmosomes |
| 87425 | ACTB-mEGFP | AICSDP-15 | mEGFP | Beta-actin | Actin filaments |
| 87426 | SEC61B-mEGFP | AICSDP-7 | mEGFP | Sec61 beta | Endoplasmic reticulum |
| 87427 | FBL-mEGFP | AICSDP-13 | mEGFP | Fibrillarin | Nucleolus (dense fibrillar component) |
| 87428 | MYH10-mEGFP | AICSDP-24 | mEGFP | Non-muscle myosin heavy chain IIB | Actomyosin bundles |
| 87429 | TJP1-mEGFP | AICSDP-23 | mEGFP | Tight junction protein ZO-1 | Tight junctions |
| 91565 | AAVS1-mEGFP | AICSDP-35 | mEGFP | NA | Cytoplasm |
| 101781 | CETN2-mTagRFP-T | AICSDP-22 | mTagRFP-T | Centrin-2 | Centrioles |
| 101782 | LAMP1-mEGFP | AICSDP-19 | mEGFP | LAMP-1 | Lysosome |
| 101783 | MAP1LC3B-mEGFP | AICSDP-25 | mEGFP | Autophagy-related protein LC3 B | Autophagosomes |
| 101784 | SLC25A17-mEGFP | AICSDP-27 | mEGFP | Peroxisomal membrane protein PMP34 | Peroxisomes |
| 101785 | TUBA1B-mTagRFP-T | AICSDP-28 | mTagRFP-T | Alpha-tubulin | Microtubules |
| 101786 | ST6GAL1-mEGFP | AICSDP-26 | mEGFP | Sialyltransferase 1 | Golgi |
| 109122 | NPM1-mEGFP | AICSDP-50 | mEGFP | Nucleophosmin | Nucleolus (granular component) |
| 109120 | GJA1-mEGFP | AICSDP-43 | mEGFP | Connexin-43 | Gap junctions |
| 109121 | HIST1H2BJ-mEGFP | AICSDP-52 | mEGFP | Histone H2B type 1-J | Histones |
| 109119 | CTNNB1-mEGFP | AICSDP-47 | mEGFP | Beta-catenin | Adherens junctions |
| 107579 | RAB5A-mEGFP | AICSDP-39 | mEGFP | Ras-related protein Rab-5A | Endosomes |
| 107580 | AAVS1-mTagRFPT-CAAX | AICSDP-42 | mTagRFPT | CAAX domain of K-Ras | Plasma Membrane |
| 114403 | LMNB1-mTagRFP-T | AICSDP-29 | mTagRFPT | Lamin B1 | Nuclear envelope |
| 114404 | AAVS1-mEGFP (PGK) | AICSDP-36 | mEGFP | NA | Cytoplasm |
| 114405 | ATP2A2-mEGFP | AICSDP-41 | mEGFP | SERCA2 | Sarcoplasmic reticulum/endoplasmic reticulum |
| 114406 | SMC1A-mEGFP | AICSDP-57 | mEGFP | SMC protein 1A | Cohesins |
| 114407 | NUP153-mEGFP | AICSDP-50 | mEGFP | Nucleoporin Nup153 | Nuclear pores |
| 114410 | FUS-mEGFP | AICSDP-64 | mEGFP | RNA-binding protein FUS | Paraspeckles/stress granules |
| 114411 | TNNI1-mEGFP | AICSDP-34 | mEGFP | Troponin I, slow skeletal type | Sarcomeric thin filaments |
| 114412 | TTN-mEGFP | AICSDP-45 | mEGFP | Titin | Sarcomeres (M-line tag) |
| 114413 | MYL7-mEGFP | AICSDP-46 | mEGFP | MLC-2a | Sarcomeric thick filaments |
| 114414 | MYL2-mEGFP | AICSDP-49 | mEGFP | MLC-2v | Sarcomeric thick filaments |
| 124606 | SOX2-mEGFP | AICSDP-60 | mEGFP | Transcription factor SOX-2 | Transcription Factor |
| 124607 | ACTN2-mEGFP | AICSDP-63 | mEGFP | Alpha-actinin-2 | Sarcomeric z-disks |
| 124608 | NPM1-mTagRFP-T | AICSDP-69 | mTagRFP-T | Nucleophosmin | Nucleolus (granular component) |
| 133963 | UBTF-HaloTag | AICSDP-80 | HaloTag | Nucleolar transcription factor UBF | Nucleolus (fibrillar center) |
| 133964 | SON-mEGFP | AICSDP-82 | mEGFP | SON | Nuclear speckles |
| 159744 | DMD-mEGFP | AICSDP-55 | mEGFP | Dystrophin | Costameres |
| 159745 | TFAM-mEGFP | AICSDP-72 | mEGFP | Mitochondrial transcription factor A (TFAM) | Mitochondrial nucleoids |
| 164499 | EZH2-mEGFP | AICSDP-83 | mEGFP | EZH2 | Polycomb repressive complex 2 |
| 164500 | POLR2A-mEGFP | AICSDP-117 | mEGFP | RPB1 | RNA polymerase II |
| 168798 | TERF2-mEGFP | AICSDP-77 | mEGFP | Telomeric repeat-binding factor 2 (TRF2) | Telomeres |
| 168799 | CTCF-mEGFP | AICSDP-144 | mEGFP | CCCTC-binding factor (CTCF) | CTCF-binding sites |
| 170818 | PCNA-mEGFP | AICSDP-116 | mEGFP | Proliferating cell nuclear antigen (PCNA) | DNA replication foci |
IPSC cell lines can be ordered from the Allen Institute for Cell Science Cell Catalog (Link opens in a new window).
Protocols
The Allen Institute for Cell Science has several protocol videos available that may be helpful for working with this plasmid collection.
References
Roberts B, Haupt A, Tucker A, Grancharova T, Arakaki J, Fuqua MA, Nelson A, Hookway C, Ludmann SA, Mueller IA, Yang R, Horwitz R, Rafelski SM, Gunawardane RN.
Mol Biol Cell. 2017 Oct 15;28(21):2854-2874. doi: 10.1091/mbc.E17-03-0209. Epub 2017 Aug 16.
(Link opens in a new window)
PubMed
All Allen Institute for Cell Science Publications (Link opens in a new window)