RNA-guided editing of bacterial genomes using CRISPR-Cas systems.
Jiang W, Bikard D, Cox D, Zhang F, Marraffini LA
Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2508.
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PubMed
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Article
A two plasmid system for editing bacterial genomes using an RNA-guided Cas9 nuclease.
Resources:
Marraffini pCRISPR protocol 125.6 KBPlasmids from Article
ID | Plasmid | Purpose |
---|---|---|
42875 | pCRISPR | A crRNA expression plasmid for targeting a specific sequence. |
42876 | pCas9 | Bacterial expression of Cas9 nuclease, tracrRNA and crRNA guide |
44505 | pCRISPR::rpsL | A crRNA expression plasmid specific to the rpsL allele. |