RNA-guided editing of bacterial genomes using CRISPR-Cas systems.
Jiang W, Bikard D, Cox D, Zhang F, Marraffini LA
Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2508.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
A two plasmid system for editing bacterial genomes using an RNA-guided Cas9 nuclease.
![Marraffini Lab pCas9](https://media.addgene.org/data/easy-thumbnails/filer_public/cms/filer_public/96/32/96324f53-a620-4ce7-a651-c2b7e611bf83/pcas9.jpg__750x167_crop_subsampling-2_upscale.png)
![Marraffini Lab pCRISPR](https://media.addgene.org/data/easy-thumbnails/filer_public/cms/filer_public/05/49/05495806-0fed-453d-af55-abde96fdbc16/pcrispr.jpg__750x165_crop_subsampling-2_upscale.png)
Resources:
Marraffini pCRISPR protocol 125.6 KBPlasmids from Article
ID | Plasmid | Purpose |
---|---|---|
42875 | pCRISPR | A crRNA expression plasmid for targeting a specific sequence. |
42876 | pCas9 | Bacterial expression of Cas9 nuclease, tracrRNA and crRNA guide |
44505 | pCRISPR::rpsL | A crRNA expression plasmid specific to the rpsL allele. |