CRISPR-Cas/RGN expression plasmids for Zebrafish
The Joung Lab recently described the use CRISPR-Cas/RNA-Guided Nuclease (RGN) technology to modify nine different sites in various endogenous zebrafish genes (Hwang & Fu et al., Nat Biotechnol. 2013).
The expression plasmids encoding the guide RNAs (gRNAs) used in these experiments are listed below. Note that gRNA produced from each of these vectors were used in conjunction with mRNA produced from the Cas9 expression vector (pMLM3613). Additional details can be found in Hwang & Fu et al., Nat Biotechnol. 2013 and users are encouraged to post questions to the Genome Engineering newsgroup (which can be joined here).
Individual plasmids can be ordered via the links below:
| ID | Plasmid |
|---|---|
| 42251 | MLM3613 (Cas9 expression vector) |
| 42241 | DR299 (gRNA targeted to zebrafish gene apoea ) |
| 42242 | DR336 (gRNA targeted to zebrafish gene drd3 ) |
| 42243 | DR279 (gRNA targeted to zebrafish gene fh [site #1]) |
| 42244 | DR302 (gRNA targeted to zebrafish gene fh [site #2]) |
| 42245 | DR338 (gRNA targeted to zebrafish gene gsk3b ) |
| 42246 | DR311 (gRNA targeted to zebrafish gene rgs4 ) |
| 42247 | DR317 (gRNA targeted to zebrafish gene th1 ) |
| 42248 | DR330 (gRNA targeted to zebrafish gene tia1l ) |
| 42249 | DR333 (gRNA targeted to zebrafish gene tph1a ) |