CRISPR Plasmids: Empty gRNA Expression Vectors
CRISPR requires you to express both a Cas protein and a target-specific gRNA in the same cell at the same time. All-in-one plasmids ensure that both the Cas and gRNA are delivered to the same cells, but are limited to the function of the Cas protein (cut, nick, activate, interfere, etc.). gRNA-only plasmids are not limited to a specific CRISPR function, but require an additional plasmid containing a Cas enzyme or cells or organisms in which Cas has been integrated into the genome.
Addgene has a large collection of empty gRNA vectors. Explore our most popular plasmids for expression of gRNA and Cas, gRNA only, or multiple gRNAs in the tables below.
Looking for non-targeting control vectors? Search our catalog for control gRNAs.
All-in-one Plasmids (gRNA and Cas)
| ID | Plasmid | Vector Type | Promoter | Selectable Marker | PI | Publication |
|---|
gRNA-only Plasmids
| ID | Plasmid | Vector Type | Promoter | Selectable Marker | PI | Publication |
|---|
Multiplex gRNA Plasmids
| ID | Plasmid | Vector Type | Promoter | Selectable Marker | PI | Publication |
|---|
Multiplex gRNA Kits
Many CRISPR plasmid kits at Addgene contain multiplex-compatible gRNA plasmids, including:
CRISPR Resources
Addgene has a large selection of other CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page. Find a comprehensive list of CRISPR resources by visiting our CRISPR reference page.
Content last reviewed: 19 December 2025
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