CRISPR Fly Design
Background
CRISPR fly design is a genome engineering project in Drosophila, consisting of transgenic cas9 fly lines and gRNA plasmids. Project tools can be used for both generating knockouts and precise modifications using HDR.
For more information about this project, please refer to the CRISPR Fly Design homepage.
gRNA plasmids
| ID | Name | Description | PI |
|---|---|---|---|
| 49408 | pCFD1-dU6:1gRNA | expression of gRNA under control of the Drosophila U6:1 promoter | Bullock |
| 49409 | pCFD2-dU6:2gRNA | expression of gRNA under control of the Drosophila U6:2 promoter | Bullock |
| 49410 | pCFD3-dU6:3gRNA | expression of gRNA under control of the Drosophila U6:3 promoter | Bullock |
| 49411 | pCFD4-U6:1_U6:3tandemgRNAs | expression of two gRNAs from Drosophila U6:1 and U6:3 | Bullock |
| 73914 | pCFD5 | multiplex gRNA plasmid | Bullock |
| 73915 | pCFD6 | tissue-specific multiplex gRNA plasmid | Bullock |
Cas9 plasmids
| ID | Name | Description | PI |
|---|---|---|---|
| 62209 | pAct-Cas9 | Expresses Cas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous genome engineering in Drosophila melanogaster. | Bullock |
| 62208 | pnos-Cas9-nos | Expresses Cas9 under control of nanos promoter and 3'UTR. For germ line restricted genome engineering in Drosophila melanogaster. | Bullock |
| 62211 | pAct-Fok1:dCas9 | Expresses Fok1:dCas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous high specificity genome engineering in Drosophila melanogaster. | Bullock |
| 62210 | pnos-Fok1:dCas9-nos | Expresses Fok1:dCas9 under control of nanos promoter and 3'UTR. For germ line restricted high specificity genome engineering in Drosophila melanogaster. | Bullock |