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Addgene

CRISPR Plasmids and Resources

Addgene is working with the leading scientists in the field to assemble the reagents and information you need to use CRISPR technology in your own lab. Browse plasmids below or check out our CRISPR resources on how to start using CRISPR in your lab.

Addgene CRISPR Resources

  • CRISPR Guide: Essential background information on CRISPR and the basics for planning your first CRISPR experiment.
  • CRISPR 101 eBook: Based on our popular CRISPR 101 blog series, we've organized a comprehensive CRISPR resource for you to download.
  • CRISPR Resources: Browse depositor protocols, find software for gRNA design and deep sequencing analysis, discover links to CRISPR forums, and more.
  • CRISPR History: Learn how CRISPR was modified from a bacterial immune system to a revolutionary tool for genome engineering.
  • CRISPR Topic Page: Want the latest news on CRISPR? Experts cover CRISPR topics on Addgene's blog.
  • Addgene's Viral Service: Request ready-to-use viral preps of select CRISPR lentiviral plasmids.
  • CRISPR Plasmid Kits: Find groups of plasmids that are used together in CRISPR experiments.
  • CRISPR Pooled Libraries: Find CRISPR libraries for knockout, activation, inhibition, and barcoding experiments.

Browse CRISPR Plasmids by Function

Genome Engineering

Cut

Wild-type Cas enzymes generate double strand breaks (DSBs).

Base Edit

Base editors precisely edit DNA without promoting DSB formation.

Nick

Mutated Cas nickase enzymes generate single-strand DNA breaks.

Prime Edit

Cas nickase enzymes fused to reverse transcriptase install new genetic information into a specific DNA site

Transcriptional Regulation

Screen

Activate

dCas9 fused to an activator peptide activates gene expression.

Interfere

dCas9 alone, or fused to a repressor peptide, inhibits transcription.

Epigenetics

dCas9 fused to an epigenetic modifier alters chromatin structure/gene expression.

Screen

CRISPR libraries are a powerful tool for genome-wide screening.

RNA Targeting

gRNAs

RNA Targeting

Type VI CRISPR systems target RNA, not DNA, across multiple systems.

RNA Editing

Cas13 enzymes fused to deaminases can edit bases in RNA.

Empty gRNA Vectors

Select an empty vector for your experiment based on a variety of factors.

gRNAs

gRNA plasmids to target specific genes.

Other Applications

Purify

dCas9 fused to an epitope tag can be used to isolate a given genomic locus.

Tag

Various CRISPR methods are available to endogenously tag your gene of interest.

Visualize

CRISPR fluorescence methods can be used to visualize genomic loci.

dCas9-FokI

dCas9 fused to FokI nuclease generates a DSB when expressed with two gRNAs.

Browse Plasmids by Model Organism

Bacteria

C. elegans

Drosophila

Mammalian

Parasites

Plant

Yeast

Xenopus

Zebrafish