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Addgene

BRAIN Armamentarium Collection

The Armamentarium for Precision Brain Cell Access is developing a collection of molecular genetic reagents to gain access to many different brain cell types. The expanded collection of Armamentarium tools will aid neuroscientists in studying circuits in laboratory animals and human tissue specimens. These technologies have the potential to transform neuroscience research by bringing the precision of molecular targeting to specific neural circuits that underlie behavior and network function.

Browse plasmids or viral preps created with support from the BRAIN Armamentarium Project below.

Plasmids

ID Plasmid Description PI

Viral Preps

ID Viral Prep Description PI (Cargo) PI (Capsid)
163505-PHPeB CN1390-rAAV-DLX2.0-minBG-SYFP2-WPRE3-BGHpA DLX2.0 (3xCore version of DLX enhancer). AAV vector for strong & rapid SYFP2 expression in forebrain GABAergic interneurons. The enhancer core has 100% seq conservation between mouse and human Jonathan Ting Viviana Gradinaru
163509-PHPeB CN1839-rAAV-hSyn1-SYFP2-10aa-H2B-WPRE3-BGHpA AAV vector for nuclear SYFP2 labeling in neurons. Useful for nuclear isolation and scRNA-seq applications. Jonathan Ting Viviana Gradinaru
164450-PHPeB CN1851-rAAV-hI56i-minBglobin-iCre-4X2C-WPRE3-BGHpA AAV vector for Cre expression in forebrain GABAergic interneurons. For use in combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors. Jonathan Ting Viviana Gradinaru
191720-PHPeB CN3038: rAAV-3xcore2_eHGT_743m-minBglobin-SYFP2-WPRE3-BGHpA Expression of SYFP2 (yellow fluorescent protein) in striatal and lateral septum cholinergic neurons Jonathan Ting Viviana Gradinaru
191728-PHPeB CN2408: rAAV-eHGT_600m-minBG-SYFP2-WPRE3-BGHpA Expression of SYFP2 (yellow fluorescent protein) in striatal and neocortical Sst-Chodl neurons Jonathan Ting Viviana Gradinaru
191706-PHPeB CN3044: rAAV-3xcore2_eHGT_779m-minBglobin-SYFP2-WPRE3-BGHpA Optimized expression of SYFP2 (yellow fluorescent protein) in striatal direct pathway medium spiny neurons (D1-MSNs) Jonathan Ting Viviana Gradinaru
199773-PHPeB CN3863: rAAV-core4-eHGT_888m-minBglobin-SYFP2-WPRE3-BGHpA Enhancer AAV for expression of SYFP2 in midbrain dopamine neurons Jonathan Ting Viviana Gradinaru
199774-PHPeB CN3905: rAAV-3xcore2_eHGT_441h-minBglobin-SYFP2-WPRE3-BGHpA Enhancer AAV for expression of SYFP2 in all striatal medium spiny neurons Jonathan Ting Viviana Gradinaru
199775-PHPeB CN3778: rAAV-eHGT_450h-minBglobin-iCre(R297T)-BGHpA Enhancer AAV for Cre recombinase expression in striatal medium spiny neurons Jonathan Ting Viviana Gradinaru
199776-PHPeB CN3779: rAAV-3xcore2_eHGT_779m-minBglobin-iCre(R297T)-BGHpA Enhancer AAV for Cre recombinase expression in striatal direct pathway D1-MSNs Jonathan Ting Viviana Gradinaru
199777-PHPeB CN3781: rAAV-eHGT_452h-minBglobin-iCre(R297T)-BGHpA Enhancer AAV for Cre recombinase expression in striatal indirect pathway D2-MSNs Jonathan Ting Viviana Gradinaru
37825-CAP-B10 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana Gradinaru
37825-CAP-B22 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana Gradinaru
37825-CAP-MaCPNS1 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana Gradinaru
37825-CAP-MaCPNS2 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana Gradinaru