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- BRAIN Armamentarium
BRAIN Armamentarium Collection
The Armamentarium for Precision Brain Cell Access is developing a collection of molecular genetic reagents to gain access to many different brain cell types. The expanded collection of Armamentarium tools will aid neuroscientists in studying circuits in laboratory animals and human tissue specimens. These technologies have the potential to transform neuroscience research by bringing the precision of molecular targeting to specific neural circuits that underlie behavior and network function.
Browse plasmids or viral preps created with support from the BRAIN Armamentarium Project below.
Plasmids
| ID | Plasmid | Description | PI |
|---|
Viral Preps
| ID | Viral Prep | Description | PI (Cargo) | PI (Capsid) | |
|---|---|---|---|---|---|
| 163505-PHPeB | CN1390-rAAV-DLX2.0-minBG-SYFP2-WPRE3-BGHpA | DLX2.0 (3xCore version of DLX enhancer). AAV vector for strong & rapid SYFP2 expression in forebrain GABAergic interneurons. The enhancer core has 100% seq conservation between mouse and human | Jonathan Ting | Viviana Gradinaru | |
| 163509-PHPeB | CN1839-rAAV-hSyn1-SYFP2-10aa-H2B-WPRE3-BGHpA | AAV vector for nuclear SYFP2 labeling in neurons. Useful for nuclear isolation and scRNA-seq applications. | Jonathan Ting | Viviana Gradinaru | |
| 164450-PHPeB | CN1851-rAAV-hI56i-minBglobin-iCre-4X2C-WPRE3-BGHpA | AAV vector for Cre expression in forebrain GABAergic interneurons. For use in combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors. | Jonathan Ting | Viviana Gradinaru | |
| 191720-PHPeB | AiP13038 - pAAV-AiE0743m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3038) | Expression of SYFP2 (yellow fluorescent protein) in striatal and lateral septum cholinergic neurons | Jonathan Ting | Viviana Gradinaru | |
| 191728-PHPeB | AiP12408 - pAAV-AiE0600m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2408) | Expression of SYFP2 (yellow fluorescent protein) in striatal and neocortical Sst-Chodl neurons | Jonathan Ting | Viviana Gradinaru | |
| 191706-PHPeB | AiP13044 - pAAV-AiE0779m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3044) | Optimized expression of SYFP2 (yellow fluorescent protein) in striatal direct pathway medium spiny neurons (D1-MSNs) | Jonathan Ting | Viviana Gradinaru | |
| 199773-PHPeB | AiP13863 - pAAV-AiE0888m_C4-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3863) | Enhancer AAV for expression of SYFP2 in midbrain dopamine neurons | Jonathan Ting | Viviana Gradinaru | |
| 199774-PHPeB | AiP13905 - pAAV-AiE0441h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3905) | Enhancer AAV for expression of SYFP2 in all striatal medium spiny neurons | Jonathan Ting | Viviana Gradinaru | |
| 199775-PHPeB | AiP13778 - pAAV-AiE0450h-minBG-iCre(R297T)-BGHpA (Alias: CN3778) | Enhancer AAV for Cre recombinase expression in striatal medium spiny neurons | Jonathan Ting | Viviana Gradinaru | |
| 199776-PHPeB | AiP13779 - pAAV-AiE0779m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779) | Enhancer AAV for Cre recombinase expression in striatal direct pathway D1-MSNs | Jonathan Ting | Viviana Gradinaru | |
| 199777-PHPeB | AiP13781 - pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA (Alias: CN3781) | Enhancer AAV for Cre recombinase expression in striatal indirect pathway D2-MSNs | Jonathan Ting | Viviana Gradinaru | |
| 37825-CAP-B10 | pAAV-CAG-GFP | AAV expression of GFP from the CAG promoter | Edward Boyden | Viviana Gradinaru | |
| 37825-CAP-B22 | pAAV-CAG-GFP | AAV expression of GFP from the CAG promoter | Edward Boyden | Viviana Gradinaru | |
| 37825-CAP-MaCPNS1 | pAAV-CAG-GFP | AAV expression of GFP from the CAG promoter | Edward Boyden | Viviana Gradinaru | |
| 37825-CAP-MaCPNS2 | pAAV-CAG-GFP | AAV expression of GFP from the CAG promoter | Edward Boyden | Viviana Gradinaru | |
| 191707-PHPeB | AiP12237 - pAAV-AiE0452h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2237) | Expression of SYFP2 (cyan fluorescent protein) in striatal indirect pathway medium spiny neurons (D2-MSNs) | Jonathan Ting | Viviana Gradinaru | |
| 191726-PHPeB | AiP12787 - pAAV-AiE0140h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2787) | Expression of SYFP2 (yellow fluorescent protein) in striatal Pvalb+ neurons. Note strong cortical Pvalb and L5 ET neuron expression. | Jonathan Ting | Viviana Gradinaru | |
| 191737-PHPeB | AiP12610 - pAAV-AiE0780m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2610) | Expression of SYFP2 (yellow fluorescent protein) in striatal direct pathway medium spiny neurons (D1-MSNs) | Jonathan Ting | Viviana Gradinaru | |
| 213814-PHPeB | pAAV_BiSSTe10_dTomato_nlsdTomato | AAV construct expressing dTomato driven by SST interneuron-targeting enhancer | Gordon Fishell | Viviana Gradinaru | |
| 213815-PHPeB | pAAV_BiSSTe10_ChR2_mCherry | AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by SST interneuron-targeting enhancer | Gordon Fishell | Viviana Gradinaru | |
| 213829-PHPeB | pAAV_BiCHATe27_dTomato_nlsdTomato | AAV construct expressing dTomato driven by cholinergic neuron-targeting enhancer | Gordon Fishell | Viviana Gradinaru | |
| 213830-PHPeB | pAAV_BiCHATe27_ChR2_mCherry | AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by cholinergic neuron-targeting enhancer | Gordon Fishell | Viviana Gradinaru | |
| 213855-PHPeB | pAAV_BiVIPe4_dTomato_nlsdTomato | AAV construct expressing dTomato driven by VIP interneuron-targeting enhancer | Gordon Fishell | Viviana Gradinaru | |
| 213856-PHPeB | pAAV_BiVIPe4_ChR2_mCherry | AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by VIP interneuron-targeting enhancer | Gordon Fishell | Viviana Gradinaru | |
| 213914-PHPeB | pAAV_BiLAMP5e3_dTomato_nlsdTomato_nlsdTomato | AAV construct expressing dTomato driven by Lamp5 interneuron-targeting enhancer | Gordon Fishell | Viviana Gradinaru | |
| 213915-PHPeB | pAAV_BiLAMP5e3_ChR2_mCherry | AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by Lamp5 interneuron-targeting enhancer | Gordon Fishell | Viviana Gradinaru | |
| 213936-PHPeB | pAAV_BiPVe4_dTomato_nlsdTomato | AAV construct expressing dTomato driven by chandelier cell-targeting enhancer | Gordon Fishell | Viviana Gradinaru | |
| 213937-PHPeB | pAAV_BiPVe4_ChR2_mCherry | AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by chandelier cell-targeting enhancer | Gordon Fishell | Viviana Gradinaru | |
| 213940-PHPeB | pAAV_BiPVe3_dTomato_nlsdTomato | AAV construct expressing dTomato driven by PV+ basket cell-targeting enhancer | Gordon Fishell | Viviana Gradinaru | |
| 213941-PHPeB | pAAV_BiPVe3_ChR2_mCherry | AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by PV+ basket cell-targeting enhancer | Gordon Fishell | Viviana Gradinaru | |
| 213944-PHPeB | pAAV_BiSSTe4_dTomato_nlsdTomato | AAV construct expressing dTomato driven by SST interneuron-targeting enhancer | Gordon Fishell | Viviana Gradinaru | |
| 213945-PHPeB | pAAV_BiSSTe4_ChR2_mCherry | AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by SST interneuron-targeting enhancer | Gordon Fishell | Viviana Gradinaru | |
| 214846-PHPeB | AiP13755: pAAV-AiE0743m_3xC2-minBG-ChR2(H134R)-EYFP-WPRE3-BGHpA (Alias: CN3755) | Expression of ChR2(H134R)-EYFP in striatal cholinergic neurons | Jonathan Ting | Viviana Gradinaru | |
| 214852-PHPeB | AiP14033: pAAV-AiE0779m_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN4033) | Expression of CoChR-EGFP in striatal direct pathway medium spiny neurons (D1 MSNs) | Jonathan Ting | Viviana Gradinaru | |
| 214853-PHPeB | AiP14035: pAAV-AiE0452h_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN4035) | Expression of CoChR-EGFP in striatal indirect pathway medium spiny neurons (D2 MSNs) | Jonathan Ting | Viviana Gradinaru | |
| 214858-PHPeB | AiP14496: pAAV-AiE0873m_3xC2-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4496) | Expression of SYFP2 (yellow fluorescent protein) plus separate nuclear FLAG epitope in striatal cholinergic neurons | Jonathan Ting | Viviana Gradinaru | |
| 214862-PHPeB | AiP14825: pAAV-AiE0441h_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN4825) | Expression of iCre(R297T) in striatal medium spiny neurons | Jonathan Ting | Viviana Gradinaru | |
| 214869-PHPeB | AiP15140: pAAV-AiE0873m_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN5140) | Expression of CoChR-EGFP in striatal cholinergic neurons | Jonathan Ting | Viviana Gradinaru |