Plasmids for ARF Superfamily Research
The ARF (ADP-ribosylation factor) family of regulatory GTPases have long been characterized as members of the RAS superfamily, though recently have been argued to form their own superfamily (Klinger, et al 2016 PMID: 26893300). Though best known for their roles as regulators of membrane traffic, they also play critical roles at cilia, centrosomes, the plasma membrane, lipid droplets, the nucleus, and mitochondria. The activity of GTPases is regulated both upstream (by GEFs, guanine nucleotide exchange factors), and downstream (by GAPs, GTPase activating proteins, which also serve effector roles). A compatible GEF, GTPase, and GAP set is referred to as a minimal triad, with each component involved in GTPase signaling activity.
Locations of ARF Superfamily members.
Figure from Szutl, et al 2019 PMID: 31084567.
More information about ARF Superfamily members can be found in: ARF GTPases and their GEFs and GAPs: concepts and challenges Sztul E, Chen PW, Casanova JE, Cherfils J, Dacks JB, Lambright DG, Lee FS, Randazzo PA, Santy LC, Schürmann A, Wilhelmi I, Yohe ME, Kahn RA. Mol Biol Cell. 2019 May 15;30(11):1249-1271. doi: 10.1091/mbc.E18-12-0820. PubMed PMID: 31084567.
The content for this page was generated with the help of Richard Kahn.
Browse ARF GTPase Plasmids by Gene
The following table lists genes in the ARF GTPase family, information on each gene, and examples of plasmid types available for each gene. Click the gene name for a full list of plasmids available. Please note that some genes may not yet have plasmids available for request.
| Family | Gene | Entrez Gene ID | Length (aa) | Example Expression Systems |
|---|---|---|---|---|
| GTPase | Arf1 | 375 | 181 | Bacterial (pET), Mammalian (pcDNA3), Gateway |
| GTPase | Arf3 | 377 | 181 | Bacterial (pET), Mammalian (pcDNA3), Gateway |
| GTPase | Arf4 | 378 | 180 | Bacterial (pET), Mammalian (pcDNA3), Gateway |
| GTPase | Arf5 | 381 | 180 | Bacterial (pET), Mammalian (pcDNA3), Gateway |
| GTPase | Arf6 | 382 | 175 | Bacterial (pET), Mammalian (pcDNA3), Gateway |
| GTPase | Arl1 | 400 | 181 | Bacterial (pET), Gateway |
| GTPase | Arl2 | 402 | 184 | Bacterial (pET), Mammalian (pcDNA3), Gateway |
| GTPase | Arl3 | 403 | 182 | Bacterial (pET), Mammalian (pcDNA3), Gateway |
| GTPase | Arl4a | 10124 | 200 | Bacterial (pET), Gateway |
| GTPase | Arl4c | 10123 | 201/192 | Gateway |
| GTPase | Arl4d | 379 | 201 | Gateway |
| GTPase | Arl5a | 26225 | 179 | Bacterial (pET), Gateway |
| GTPase | Arl5b | 221079 | 179 | Bacterial (pET), Gateway |
| GTPase | Arl5c | 390790 | 179 | |
| GTPase | Arl6 | 84100 | 186 | Bacterial (pET), Gateway |
| GTPase | Arl8a | 127829 | 186 | Bacterial (pET), Gateway |
| GTPase | Arl8b | 55207 | 186 | Bacterial (pET), Gateway |
| GTPase | Arl9 | 132946 | 187 | |
| GTPase | Arl10 | 285598 | 276; 244 | |
| GTPase | Arl11 | 115761 | 196 | Bacterial (pET), Gateway |
| GTPase | Arl13a | 392509 | 256 | |
| GTPase | Arl13b | 200894 | 428 | Mammalian (pcDNA3), Gateway |
| GTPase | Arl14 | 80117 | 192 | Bacterial (pET), Gateway |
| GTPase | Arl15 | 54622 | 204 | |
| GTPase | Arl16 | 339231 | 197 | Bacterial (pET) |
| GTPase | Arfrp1 | 10139 | 201 | Gateway |
| GTPase | Sar1a | 56681 | 198 | Bacterial (pET), Gateway |
| GTPase | Sar1b | 51128 | 198 | Gateway |
| GTPase | Trim23 | 373 | 574 |
Browse ARF GAP and GEF Plasmids by Gene
The following table lists genes in the ARF GAP and GEF family, information on each gene, and examples of plasmid types available for each gene. Click the gene name for a full list of plasmids available. Please note that some genes may not yet have plasmids available for request.
| Family | Gene | Entrez Gene ID | Length (aa) | Example Expression Systems |
|---|---|---|---|---|
| GAP | Arfgap1 | 55738 | 414 | Bacterial (pET) |
| GAP | Arfgap2 | 84364 | 521 | Bacterial (pET) |
| GAP | Arfgap3 | 26286 | 516 | |
| GAP | Asap1 | 5080 | 1129 | |
| GAP | Asap2 | 8853 | 1006 | |
| GAP | Asap3 | 55616 | 903 | Bacterial (pET) |
| GAP | Arap1 | 116985 | 1450 | |
| GAP | Arap2 | 116984 | 1704 | |
| GAP | Arap3 | 64411 | 1544 | Mammalian (pEGFP-C2) |
| GAP | Acap1 | 9744 | 740 | Mammalian (pFLAG-CMV2) |
| GAP | Acap2 | 23527 | 778 | |
| GAP | Acap3 | 116983 | 834 | |
| GAP | Agap1 | 116987 | 857 | |
| GAP | Agap2 | 116986 | 1192 | Bacterial (pET) |
| GAP | Agap3 | 116988 | 911 | Bacterial (pET) |
| GAP | Adap1 | 11033 | 374 | Bacterial (pET) |
| GAP | Adap2 | 55803 | 387 | |
| GAP | Smap1 | 60682 | 467 | |
| GAP | Smap2 | 64744 | 429 | Bacterial (pET) |
| GAP | Git1 | 28964 | 770 | |
| GAP | Git2 | 9815 | 759 | |
| GAP | ELMOD1 | 55531 | 334 | |
| GAP | ELMOD2 | 255550 | 293 | Mammalian (pcDNA3) |
| GAP | ELMOD3 | 84173 | 381 | Mammalian (pcDNA3) |
| GEF | Arfgef1 | 10565 | 1849 | Mammalian (pcDNA4c) |
| GEF | Arfgef2 | 10564 | 1785 | |
| GEF | Iqsec1 | 9922 | 1114 | Gateway |
| GEF | Iqsec2 | 23096 | 1488 | Bacterial (pET) |
| GEF | Iqsec3 | 440073 | 1182 | |
| GEF | Cyth1 | 9267 | 398 | Gateway |
| GEF | Cyth2 | 9266 | 400 | Mammalian (pEGFP-C1), Gateway |
| GEF | Cyth3 | 9265 | 399 | Gateway |
| GEF | Cyth4 | 27128 | 394 | Gateway |
| GEF | Gbf1 | 8729 | 1859 | Gateway |
| GEF | Psd | 5662 | 1024 | |
| GEF | Psd2 | 84249 | 771 | Gateway |
| GEF | Psd3 | 23362 | 1047 | Gateway |
| GEF | Psd4 | 23550 | 1056 | |
| GEF | Fbxo8 | 26269 | 319 | Gateway |
Do you have suggestions for other plasmids that should be added to this list?
Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this resource!