Addgene p3xP3-EGFP.vas-int.NLS Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: p3xP3-EGFP.vas-int.NLS Sequencing Result


Map View

800 600 400 200 End (834) BpmI (687) TatI (670) NmeAIII (598) EXFP-R (549 .. 568) PfoI * (523) BtsI - BtsαI (456) BssSI BssSαI (423) BtgZI (364) EGFP-N (288 .. 309) BanI (278) BseRI (273) BtgI - StyI - NcoI (241) AgeI - BsaWI (230) AvaI - SmlI - XhoI - BsoBI - PaeR7I (224) BsaI (221) PvuII - MspA1I (202) BmgBI (165) AflIII (162) AccI (124) HaeII (119) BstBI (84) ClaI - BspDI (78) HindIII (71) BamHI (65) ApaI - BanII (16) PspOMI (12) BsaAI - SnaBI (6) Start (0) VN173 Kozak sequence p3xP3-EGFP.vas-int.NLS 834 bp

Sequence View

Start (0) SnaBIBsaAIPspOMIBanIIApaI 60 g g g t a c g t a c c g g g c c c g g a t c t a a t t c a a t t a g a g a c t a a t t c a a t t a g a g c t a a t t c a c c c a t g c a t g g c c c g g g c c t a g a t t a a g t t a a t c t c t g a t t a a g t t a a t c t c g a t t a a g t

HaeIIBstBIBspDIClaIHindIIIBamHI 120 *RNRVRSRRLIFLPA a t t a g g a t c c a a g c t t a t c g a t t t c g a a c c c t c g a c c g c c g g a g t a t a a a t a g a g g c g c t t a a t c c t a g g t t c g a a t a g c t a a a g c t t g g g a g c t g g c g g c c t c a t a t t t a t c t c c g c g a

AflIIIBmgBIAccI 180 EDVSRCNLEFLCLSCTALRF t c g t c t a c g g a g c g a c a a t t c a a t t c a a a c a a g c a a a g t g a a c a c g t c g c t a a g c g a a a g a g c a g a t g c c t c g c t g t t a a g t t a a g t t t g t t c g t t t c a c t t g t g c a g c g a t t c g c t t t c

BsaWIAgeIBsaIPaeR7IBsoBIXhoISmlIAvaIMspA1IPvuII 240 Kozak sequence SLLYVLAASCALCDARSRDG c t a a g c a a a t a a a c a a g c g c a g c t g a a c a a g c t a a a c a a t c g g c t c g a g a c c g g t c g c c a g a t t c g t t t a t t t g t t c g c g t c g a c t t g t t c g a t t t g t t a g c c g a g c t c t g g c c a g c g g t

BanIBseRINcoIStyIBtgI 300 1 M 1a V 5 10 15 SKGEELFTGVVPILVEL VN173 Kozak sequence G A C C A G C T C G A C C EGFP-N MVSKGEELFTGVVPILVEL GHHALALLQEGPHHGDQDLQ c c a t g g t g a g c a a g g g c g a g g a g c t g t t c a c c g g g g t g g t g c c c a t c c t g g t c g a g c t g g g g t a c c a c t c g t t c c c g c t c c t c g a c a a g t g g c c c c a c c a c g g g t a g g a c c a g c t c g a c c

360 20 25 30 35 DGDVNGHKFSVSGEGEGDAT VN173 T G C C G C T G C EGFP-N DGDVNGHKFSVSGEGEGDAT VAVYVAVLEAHGALALAIGG a c g g c g a c g t a a a c g g c c a c a a g t t c a g c g t g t c c g g c g a g g g c g a g g g c g a t g c c a c c t t g c c g c t g c a t t t g c c g g t g t t c a a g t c g c a c a g g c c g c t c c c g c t c c c g c t a c g g t g g a

BtgZI 420 40 45 50 55 YGKLTLKFICTTGKLPVPWP VN173 YGKLTLKFICTTGKLPVPWP VALQGQLEDAGGALQGHGPG a c g g c a a g c t g a c c c t g a a g t t c a t c t g c a c c a c c g g c a a g c t g c c c g t g c c c t g g c c c a t g c c g t t c g a c t g g g a c t t c a a g t a g a c g t g g t g g c c g t t c g a c g g g c a c g g g a c c g g g t

BtsαIBtsIBssSαIBssSI 480 60 65 70 75 TLVTTLTYGVQCFSRYPDHM VN173 TLVTTLTYGVQCFSRYPDHM GEHGGQGVAHLAEAAVGVVH c c c t c g t g a c c a c c c t g a c c t a c g g c g t g c a g t g c t t c a g c c g c t a c c c c g a c c a c a t g a g g g a g c a c t g g t g g g a c t g g a t g c c g c a c g t c a c g a a g t c g g c g a t g g g g c t g g t g t a c t

PfoI* 540 80 85 90 95 KQHDFFKSAMPEGYVQERTI VN173 KQHDFFKSAMPEGYVQERTI LLVVEELGGHGFAVDLLAGD a g c a g c a c g a c t t c t t c a a g t c c g c c a t g c c c g a a g g c t a c g t c c a g g a g c g c a c c a t c t t c g t c g t g c t g a a g a a g t t c a g g c g g t a c g g g c t t c c g a t g c a g g t c c t c g c g t g g t a g a

NmeAIII 600 100 105 110 115 FFKDDGNYKTRAEVKFEGDT VN173 C T G C T G C C G T T G A T G T T C T G EXFP-R FFKDDGNYKTRAEVKFEGDT EELVVAVVLGAGLHLELAVG t c t t c a a g g a c g a c g g c a a c t a c a a g a c c c g c g c c g a g g t g a a g t t c g a g g g c g a c a c c c a g a a g t t c c t g c t g c c g t t g a t g t t c t g g g c g c g g c t c c a c t t c a a g c t c c c g c t g t g g g

660 120 125 130 135 LVNRIELKGIDFKEDGNILG VN173 LVNRIELKGIDFKEDGNILG QHVADLQLADVELLVAVDQP t g g t g a a c c g c a t c g a g c t g a a g g g c a t c g a c t t c a a g g a g g a c g g c a a c a t c c t g g g g c a c c a c t t g g c g t a g c t c g a c t t c c c g t a g c t g a a g t t c c t c c t g c c g t t g t a g g a c c c c g

BpmITatI 720 140 145 150 155 HKLEYNYNSHNVYIMADKQK VN173 HKLEYNYNSHNVYIMADKQK VLQLVVVVAVVDIDHGVLLL a c a a g c t g g a g t a c a a c t a c a a c a g c c a c a a c g t c t a t a t c a t g g c c g a c a a g c a g a a g a t g t t c g a c c t c a t g t t g a t g t t g t c g g t g t t g c a g a t a t a g t a c c g g c t g t t c g t c t t c t

780 160 165 170 NGIKVNFKIRHNIE (in frame with VN173) DGSVQL VN173 NGIKVNFKIRHNIEDGSVQL VADLHVELDAVVDLVAAHLE a c g g c a t c a a g g t g a a c t t c a a g a t c c g c c a c a a c a t c g a g g a c g g c a g c g t g c a g c t c g t g c c g t a g t t c c a c t t g a a g t t c t a g g c g g t g t t g t a g c t c c t g c c g t c g c a c g t c g a g c

End (834) 834 (in frame with VN173) ADHYQQNTPIGDGPVLLP ADHYQQNTPIGDGPVLLP GVVVLLVGGDAVAGHQQG c c g a c c a c t a c c a g c a g a a c a c c c c c a t c g g c g a c g g c c c c g t g c t g c t g c c c g g g c t g g t g a t g g t c g t c t t g t g g g g g t a g c c g c t g c c g g g g c a c g a c g a c g g g c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.