Addgene pMTB-Multibow-G Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pMTB-Multibow-G Sequencing Result

Map View

800 600 400 200 End (960) DraI (955) BsaBI * (915) XbaI * (907) BsiEI (900) NotI - EagI (897) BsiHKAI (706) BmrI (702) EXFP-R (403 .. 422) PfoI * (377) BtsI - BtsĪ±I (310) BssSI - BssSĪ±I (277) BsrFI (247) BtgZI (218) EGFP-N (142 .. 163) BanI (132) BseRI (127) BsrDI (101) PvuII - BfuAI - PaqCI - BspMI - MspA1I (76) HaeII (73) StyI - BtgI - NcoI (62) BclI * (45) BciVI (18) Start (0) lox2272 Kozak sequence EGFP EGFP pMTB-Multibow-G 960 bp

Sequence View

BclI*BciVIStart (0) 5Ź¹ 3Ź¹ 60 lox2272 Kozak sequence g g g a t c t a g g a t a a c t t c g t a t a g g a t a c t t t a t a c g a a g t t a t t g a t c a a t t c a c a g c c c c c t a g a t c c t a t t g a a g c a t a t c c t a t g a a a t a t g c t t c a a t a a c t a g t t a a g t g t c g g

BsrDIHaeIIMspA1IBspMIPaqCIPvuIIBfuAINcoIStyIBtgI 120 1 M 1a V 5 SKGEEL Kozak sequence EGFP 1 M 1a V 5 SKGEEL EGFP MGAPAGGAGAAMVSKGEEL a c c a t g g g a g c g c c a g c t g g a g g t g c a g g t g c a g c a a t g g t g a g c a a g g g c g a g g a g c t g t g g t a c c c t c g c g g t c g a c c t c c a c g t c c a c g t c g t t a c c a c t c g t t c c c g c t c c t c g a c

BanIBseRI 180 10 15 20 25 FTGVVPILVELDGDVNGHKF EGFP 10 15 20 25 FTGVVPILVELDGDVNGHKF EGFP G A C C A G C T C G A C C T G C C G C T G C EGFP-N FTGVVPILVELDGDVNGHKF t t c a c c g g g g t g g t g c c c a t c c t g g t c g a g c t g g a c g g c g a c g t a a a c g g c c a c a a g t t c a a g t g g c c c c a c c a c g g g t a g g a c c a g c t c g a c c t g c c g c t g c a t t t g c c g g t g t t c a a g

BtgZI 240 30 35 40 45 SVSGEGEGDATYGKLTLKFI EGFP 30 35 40 45 SVSGEGEGDATYGKLTLKFI EGFP SVSGEGEGDATYGKLTLKFI a g c g t g t c c g g c g a g g g c g a g g g c g a t g c c a c c t a c g g c a a g c t g a c c c t g a a g t t c a t c t c g c a c a g g c c g c t c c c g c t c c c g c t a c g g t g g a t g c c g t t c g a c t g g g a c t t c a a g t a g

BssSĪ±IBssSIBsrFI 300 50 55 60 65 CTTGKLPVPWPTLVTTLTYG EGFP 50 55 60 65 CTTGKLPVPWPTLVTTLTYG EGFP CTTGKLPVPWPTLVTTLTYG t g c a c c a c c g g c a a g c t g c c c g t g c c c t g g c c c a c c c t c g t g a c c a c c c t g a c c t a c g g c a c g t g g t g g c c g t t c g a c g g g c a c g g g a c c g g g t g g g a g c a c t g g t g g g a c t g g a t g c c g

BtsĪ±IBtsI 360 70 75 80 85 VQCFSRYPDHMKQHDFFKSA EGFP 70 75 80 85 VQCFSRYPDHMKQHDFFKSA EGFP VQCFSRYPDHMKQHDFFKSA g t g c a g t g c t t c a g c c g c t a c c c c g a c c a c a t g a a g c a g c a c g a c t t c t t c a a g t c c g c c c a c g t c a c g a a g t c g g c g a t g g g g c t g g t g t a c t t c g t c g t g c t g a a g a a g t t c a g g c g g

PfoI* 420 90 95 100 105 MPEGYVQERTIFFKDDGNYK EGFP 90 95 100 105 MPEGYVQERTIFFKDDGNYK EGFP C T G C T G C C G T T G A T G T T C EXFP-R MPEGYVQERTIFFKDDGNYK a t g c c c g a a g g c t a c g t c c a g g a g c g c a c c a t c t t c t t c a a g g a c g a c g g c a a c t a c a a g t a c g g g c t t c c g a t g c a g g t c c t c g c g t g g t a g a a g a a g t t c c t g c t g c c g t t g a t g t t c

480 110 115 120 125 TRAEVKFEGDTLVNRIELKG EGFP 110 115 120 125 TRAEVKFEGDTLVNRIELKG EGFP T G EXFP-R TRAEVKFEGDTLVNRIELKG a c c c g c g c c g a g g t g a a g t t c g a g g g c g a c a c c c t g g t g a a c c g c a t c g a g c t g a a g g g c t g g g c g c g g c t c c a c t t c a a g c t c c c g c t g t g g g a c c a c t t g g c g t a g c t c g a c t t c c c g

540 130 135 140 145 IDFKEDGNILGHKLEYNYNS EGFP 130 135 140 145 IDFKEDGNILGHKLEYNYNS EGFP IDFKEDGNILGHKLEYNYNS a t c g a c t t c a a g g a g g a c g g c a a c a t c c t g g g g c a c a a g c t g g a g t a c a a c t a c a a c a g c t a g c t g a a g t t c c t c c t g c c g t t g t a g g a c c c c g t g t t c g a c c t c a t g t t g a t g t t g t c g

600 150 155 160 165 HNVYIMADKQKNGIKVNFKI EGFP 150 155 160 165 HNVYIMADKQKNGIKVNFKI EGFP HNVYIMADKQKNGIKVNFKI c a c a a c g t c t a t a t c a t g g c c g a c a a g c a g a a g a a c g g c a t c a a g g t g a a c t t c a a g a t c g t g t t g c a g a t a t a g t a c c g g c t g t t c g t c t t c t t g c c g t a g t t c c a c t t g a a g t t c t a g

660 170 175 180 185 RHNIEDGSVQLADHYQQNTP EGFP 170 175 180 185 RHNIEDGSVQLADHYQQNTP EGFP RHNIEDGSVQLADHYQQNTP c g c c a c a a c a t c g a g g a c g g c a g c g t g c a g c t c g c c g a c c a c t a c c a g c a g a a c a c c c c c g c g g t g t t g t a g c t c c t g c c g t c g c a c g t c g a g c g g c t g g t g a t g g t c g t c t t g t g g g g g

BmrIBsiHKAI 720 190 195 200 205 IGDGPVLLPDNHYLSTQSAL EGFP 190 195 200 205 IGDGPVLLPDNHYLSTQSAL EGFP IGDGPVLLPDNHYLSTQSAL a t c g g c g a c g g c c c c g t g c t g c t g c c c g a c a a c c a c t a c c t g a g c a c c c a g t c c g c c c t g t a g c c g c t g c c g g g g c a c g a c g a c g g g c t g t t g g t g a t g g a c t c g t g g g t c a g g c g g g a c

780 210 215 220 225 SKDPNEKRDHMVLLEFVTAA EGFP 210 215 220 225 SKDPNEKRDHMVLLEFVTAA EGFP SKDPNEKRDHMVLLEFVTAA a g c a a a g a c c c c a a c g a g a a g c g c g a t c a c a t g g t c c t g c t g g a g t t c g t g a c c g c c g c c t c g t t t c t g g g g t t g c t c t t c g c g c t a g t g t a c c a g g a c g a c c t c a a g c a c t g g c g g c g g

840 230 235 GITLGMDELYK AAA* EGFP 230 235 GITLGMDELYK AAA* EGFP GITLGMDELYKAAA* g g g a t c a c t c t c g g c a t g g a c g a g c t g t a c a a g g c a g c a g c a t g a t a a a t g g t c c t g c t g c c c t a g t g a g a g c c g t a c c t g c t c g a c a t g t t c c g t c g t c g t a c t a t t t a c c a g g a c g a c

EagINotIBsiEI 900 g a g t t c g t g a c c g c c g c c g g g a t c a c t c t c g g c a t g g a c g a g c t g t a c a a g t a a a g c g g c c t c a a g c a c t g g c g g c g g c c c t a g t g a g a g c c g t a c c t g c t c g a c a t g t t c a t t t c g c c g

End (960) DraIBsaBI*XbaI* 3Ź¹ 5Ź¹ 960 c g c g a c t c t a g a t c a t a a t c a g c c a t a c c a c a t t t g t a g a g g t t t t a c t t g c t t t a a a a a g c g c t g a g a t c t a g t a t t a g t c g g t a t g g t g t a a a c a t c t c c a a a a t g a a c g a a a t t t t t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.