Addgene pcDNA4TO-K560-E236A-24xGCN4_v1-IRES-Puro - Sequence Analyzer Skip to main content
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Sequence Analyzer: pcDNA4TO-K560-E236A-24xGCN4_v1-IRES-Puro

Map View

NruI (208) pRS-marker (44 .. 63) AmpR promoter Amp-R (9584 .. 9603) FspI (9256) AhdI (9034) pBR322ori-F (8285 .. 8304) L4440 (8034 .. 8051) BspQI - SapI (8028) CAP binding site lac promoter M13/pUC Reverse (7816 .. 7838) lac operator M13 Reverse (7803 .. 7819) M13 rev BstZ17I (7765) EBV-rev (7724 .. 7743) SV40 poly(A) signal SV40pA-R (7670 .. 7689) FseI (7405) SgrAI (7243) MauBI (7165) EM7 promoter SV40pro-F (6928 .. 6947) SV40 promoter pBABE 3' (6681 .. 6701) F1ori-F (6541 .. 6562) F1ori-R (6331 .. 6350) bGH poly(A) signal BGH-rev (5968 .. 5985) PshAI (5933) PaeR7I - PspXI - XhoI (5931) Puro-F (5827 .. 5847) BstEII (5465) BsiWI (5387) Puro-R (5331 .. 5350) PflMI (5197) IRES-F (5138 .. 5157) BfuAI - BspMI - PaqCI (5083) IRES reverse (4911 .. 4928) BmtI (4740) NdeI (484) SnaBI (590) CMV-F (720 .. 740) CMVd3 promoter tet operator tet operator LNCX (820 .. 844) BspEI (906) BamHI (947) Kozak sequence AleI (1073) PpuMI (1387) BbvCI (1764) HpaI (1962) PstI (1969) BsrGI (2689) GCN4_v1 GCN4_v1 GCN4_v1 GCN4_v1 GCN4_v1 GCN4_v1 GCN4_v1 GCN4_v1 GCN4_v1 GCN4_v1 GCN4_v1 GCN4_v1 pGEX 5' (3692 .. 3714) pGEX 3' (3715 .. 3737) GCN4_v1 GCN4_v1 GCN4_v1 GCN4_v1 GCN4_v1 GCN4_v1 GCN4_v1 GCN4_v1 GCN4_v1 GCN4_v1 GCN4_v1 GCN4_v1 EcoRI (4721) NheI (4736) pcDNA4TO-K560-E236A-24xGCN4_v1-IRES-Puro 9957 bp

Sequence View

5ʹ 3ʹ 60 A T G T T A G A C G A G A C T A C pRS-marker g a c g g a t c g g g a g a t c t c c c g a t c c c c t a t g g t g c a c t c t c a g t a c a a t c t g c t c t g a t g c t g c c t a g c c c t c t a g a g g g c t a g g g g a t a c c a c g t g a g a g t c a t g t t a g a c g a g a c t a c

120 G G C pRS-marker c c g c a t a g t t a a g c c a g t a t c t g c t c c c t g c t t g t g t g t t g g a g g t c g c t g a g t a g t g c g g g c g t a t c a a t t c g g t c a t a g a c g a g g g a c g a a c a c a c a a c c t c c a g c g a c t c a t c a c g c

180 c g a g c a a a a t t t a a g c t a c a a c a a g g c a a g g c t t g a c c g a c a a t t g c a t g a a g a a t c t g c g c t c g t t t t a a a t t c g a t g t t g t t c c g t t c c g a a c t g g c t g t t a a c g t a c t t c t t a g a c g

NruI 240 CMV enhancer t t a g g g t t a g g c g t t t t g c g c t g c t t c g c g a t g t a c g g g c c a g a t a t a c g c g t t g a c a t t a a t c c c a a t c c g c a a a a c g c g a c g a a g c g c t a c a t g c c c g g t c t a t a t g c g c a a c t g t a a

300 CMV enhancer g a t t a t t g a c t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a t a c t a a t a a c t g a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t a t

360 CMV enhancer t g g a g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c a c c t c a a g g c g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g

420 CMV enhancer c c c g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c g g g c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g

480 CMV enhancer a t t g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t t a a c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a

NdeI 540 CMV enhancer a t c a t a t g c c a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t t a g t a t a c g g t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a

SnaBI 600 CMV enhancer a t g c c c a g t a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t a c g g g t c a t g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t

660 CMV enhancer t c g c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t t g a g c g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a a c

720 C CMV-F a c t c a c g g g g a t t t c c a a g t c t c c a c c c c a t t g a c g t c a g c t c a g c t t t c t a g a c c g c g c t g a g t g c c c c t a a a g g t t c a g a g g t g g g g t a a c t g c a g t c g a g t c g a a a g a t c t g g c g c g

780 CMVd3 promoter tet operator G C A A A T G G G C G G T A G G C G T G CMV-F MGGRRVRWEVYISRALPIS g c a a a t g g g c g g t a g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g a g c t c t c c c t a t c a g c g t t t a c c c g c c a t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c t c g a g a g g g a t a g t c

840 tet operator tet operator A G C T C G T T T A G T G A A C C G T C A LNCX DRDLPISDRDRRRARLVNRQ t g a t a g a g a t c t c c c t a t c a g t g a t a g a g a t c g t c g a c g a g c t c g t t t a g t g a a c c g t c a a c t a t c t c t a g a g g g a t a g t c a c t a t c t c t a g c a g c t g c t c g a g c a a a t c a c t t g g c a g t

900 G A T C LNCX IAWRRHPRCFDLHRRHRDRS g a t c g c c t g g a g a c g c c a t c c a c g c t g t t t t g a c c t c c a t a g a a g a c a c c g g g a c c g a t c c t a g c g g a c c t c t g c g g t a g g t g c g a c a a a a c t g g a g g t a t c t t c t g t g g c c c t g g c t a g

BamHIBspEI 960 Kozak sequence SLRTLAFKLKLGTELGSATM c a g c c t c c g g a c t c t a g c g t t t a a a c t t a a g c t t g g t a c c g a g c t c g g a t c c g c c a c c a t g t c g g a g g c c t g a g a t c g c a a a t t t g a a t t c g a a c c a t g g c t c g a g c c t a g g c g g t g g t a

1020 Kozak sequence ADLAECNIKVMCRFRPLNES g g c g g a c c t g g c c g a g t g c a a c a t c a a a g t g a t g t g t c g c t t c a g a c c t c t c a a c g a g t c c c g c c t g g a c c g g c t c a c g t t g t a g t t t c a c t a c a c a g c g a a g t c t g g a g a g t t g c t c a g

AleI 1080 EVNRGDKYIAKFQGEDTVVI t g a a g t g a a c c g c g g c g a c a a g t a c a t c g c c a a g t t t c a g g g a g a a g a c a c g g t c g t g a t a c t t c a c t t g g c g c c g c t g t t c a t g t a g c g g t t c a a a g t c c c t c t t c t g t g c c a g c a c t a

1140 ASKPYAFDRVFQSSTSQEQV c g c g t c c a a g c c t t a t g c a t t t g a t c g g g t g t t c c a g t c a a g c a c a t c t c a a g a g c a a g t g c g c a g g t t c g g a a t a c g t a a a c t a g c c c a c a a g g t c a g t t c g t g t a g a g t t c t c g t t c a

1200 YNDCAKKIVKDVLEGYNGTI g t a t a a t g a c t g t g c a a a g a a g a t t g t t a a a g a t g t a c t t g a a g g a t a t a a t g g a a c a a t c a t a t t a c t g a c a c g t t t c t t c t a a c a a t t t c t a c a t g a a c t t c c t a t a t t a c c t t g t t a

1260 FAYGQTSSGKTHTMEGKLHD a t t t g c a t a c g g c c a a a c a t c c t c t g g g a a g a c a c a c a c a a t g g a g g g t a a a c t t c a t g a t a a a c g t a t g c c g g t t t g t a g g a g a c c c t t c t g t g t g t g t t a c c t c c c a t t t g a a g t a c t

1320 PEGMGIIPRIVQDIFNYIYS t c c a g a a g g c a t g g g a a t t a t t c c a a g a a t a g t g c a a g a t a t t t t t a a t t a t a t t t a c t c a g g t c t t c c g t a c c c t t a a t a a g g t t c t t a t c a c g t t c t a t a a a a a t t a a t a t a a a t g a g

1380 MDENLEFHIKVSYFEIYLDK g a t g g a t g a a a a t t t g g a a t t t c a t a t t a a g g t t t c a t a t t t t g a a a t a t a t t t g g a t a a c t a c c t a c t t t t a a a c c t t a a a g t a t a a t t c c a a a g t a t a a a a c t t t a t a t a a a c c t a t t

PpuMI 1440 IRDLLDVSKTNLSVHEDKNR g a t a a g g g a c c t g t t a g a t g t t t c a a a g a c c a a c c t t t c a g t t c a t g a a g a c a a a a a c c g c t a t t c c c t g g a c a a t c t a c a a a g t t t c t g g t t g g a a a g t c a a g t a c t t c t g t t t t t g g c

1500 VPYVKGCTERFVCSPDEVMD a g t t c c c t a t g t a a a g g g g t g c a c a g a g c g t t t t g t a t g t a g t c c a g a t g a a g t t a t g g a t c a a g g g a t a c a t t t c c c c a c g t g t c t c g c a a a a c a t a c a t c a g g t c t a c t t c a a t a c c t

1560 TIDEGKSNRHVAVTNMNEHS t a c c a t a g a t g a a g g a a a a t c c a a c a g a c a t g t a g c a g t t a c a a a t a t g a a t g a a c a t a g a t g g t a t c t a c t t c c t t t t a g g t t g t c t g t a c a t c g t c a a t g t t t a t a c t t a c t t g t a t c

1620 SRSHSIFLINVKQENTQTEQ c t c t a g g a g t c a c a g t a t a t t t c t t a t t a a t g t c a a a c a a g a g a a c a c a c a a a c g g a a c a g a g a t c c t c a g t g t c a t a t a a a g a a t a a t t a c a g t t t g t t c t c t t g t g t g t t t g c c t t g t

1680 KLSGKLYLVDLAGSAKVSKT a a a g c t g a g t g g a a a a c t t t a t c t g g t t g a t t t a g c t g g t a g t g c g a a g g t t a g t a a a a c t t t c g a c t c a c c t t t t g a a a t a g a c c a a c t a a a t c g a c c a t c a c g c t t c c a a t c a t t t t g

1740 GAEGAVLDEAKNINKSLSAL t g g a g c t g a a g g t g c t g t g c t g g a t g a a g c t a a a a a c a t c a a c a a g t c a c t t t c t g c t c t a c c t c g a c t t c c a c g a c a c g a c c t a c t t c g a t t t t t g t a g t t g t t c a g t g a a a g a c g a g a

BbvCI 1800 GNVISALAEGSTYVPYRDSK t g g a a a t g t t a t t t c t g c t t t g g c t g a g g g t a g t a c t t a t g t t c c a t a t c g a g a t a g t a a a c c t t t a c a a t a a a g a c g a a a c c g a c t c c c a t c a t g a a t a c a a g g t a t a g c t c t a t c a t t

1860 MTRILQDSLGGNCRTTIVIC a a t g a c a a g a a t c c t t c a a g a t t c a t t a g g t g g c a a c t g t a g a a c c a c t a t t g t a a t t t g t t a c t g t t c t t a g g a a g t t c t a a g t a a t c c a c c g t t g a c a t c t t g g t g a t a a c a t t a a a c

1920 CSPSSYNESETKSTLLFGQR c t g c t c t c c a t c a t c a t a c a a t g a g t c t g a a a c a a a a t c t a c a c t c t t a t t t g g c c a a a g g a c g a g a g g t a g t a g t a t g t t a c t c a g a c t t t g t t t t a g a t g t g a g a a t a a a c c g g t t t c

PstIHpaI 1980 AKTIKNTVCVNVELTAEQWK a g c c a a a a c a a t t a a g a a c a c a g t t t g t g t c a a t g t g g a g t t a a c t g c a g a a c a g t g g a a t c g g t t t t g t t a a t t c t t g t g t c a a a c a c a g t t a c a c c t c a a t t g a c g t c t t g t c a c c t t

2040 KKYEKEKEKNKILRNTIQWL a a a g a a g t a t g a a a a a g a a a a a g a a a a a a a t a a g a t c c t g c g g a a c a c t a t t c a g t g g c t t t t c t t c a t a c t t t t t c t t t t t c t t t t t t t a t t c t a g g a c g c c t t g t g a t a a g t c a c c g a

2100 ENELNRWRNGETVPIDEQFD t g a a a a t g a g c t c a a c a g a t g g c g t a a t g g g g a g a c g g t g c c t a t t g a t g a a c a g t t t g a a c t t t t a c t c g a g t t g t c t a c c g c a t t a c c c c t c t g c c a c g g a t a a c t a c t t g t c a a a c t

2160 KEKANLEAFTVDKDITLTND c a a a g a g a a a g c c a a c t t g g a a g c t t t c a c a g t g g a t a a a g a t a t t a c t c t t a c c a a t g a g t t t c t c t t t c g g t t g a a c c t t c g a a a g t g t c a c c t a t t t c t a t a a t g a g a a t g g t t a c t

2220 KPATAIGVIGNFTDAERRKC t a a a c c a g c a a c c g c a a t t g g a g t t a t a g g a a a t t t t a c t g a t g c t g a a a g a a g a a a g t g a t t t g g t c g t t g g c g t t a a c c t c a a t a t c c t t t a a a a t g a c t a c g a c t t t c t t c t t t c a c

2280 EEEIAKLYKQLDDKDEEINQ t g a a g a a g a a a t t g c t a a a t t a t a c a a a c a g c t t g a t g a c a a g g a t g a a g a a a t t a a c c a a c t t c t t c t t t a a c g a t t t a a t a t g t t t g t c g a a c t a c t g t t c c t a c t t c t t t a a t t g g t

2340 QSQLVEKLKTQMLDQEELLA g c a a a g t c a a c t g g t a g a g a a g c t g a a g a c g c a a a t g t t g g a t c a g g a g g a g c t t t t g g c c g t t t c a g t t g a c c a t c t c t t c g a c t t c t g c g t t t a c a a c c t a g t c c t c c t c g a a a a c c g

2400 STRRDQDNMQAELNRLQAEN a t c t a c c a g a a g g g a t c a a g a c a a t a t g c a a g c t g a g c t g a a t c g c c t t c a a g c a g a a a a t a g a t g g t c t t c c c t a g t t c t g t t a t a c g t t c g a c t c g a c t t a g c g g a a g t t c g t c t t t t

2460 DASKEEVKEVLQALEELAVN t g a t g c c t c t a a a g a a g a a g t g a a a g a a g t t t t a c a g g c c c t a g a a g a a c t t g c t g t c a a a c t a c g g a g a t t t c t t c t t c a c t t t c t t c a a a a t g t c c g g g a t c t t c t t g a a c g a c a g t t

2520 YDQKSQEVEDKTKEYELLSD t t a t g a t c a g a a g t c t c a g g a a g t t g a a g a c a a a a c t a a g g a a t a t g a a t t g c t t a g t g a a a t a c t a g t c t t c a g a g t c c t t c a a c t t c t g t t t t g a t t c c t t a t a c t t a a c g a a t c a c t

2580 ELNQESATLASIDAELQKLK t g a a t t g a a t c a g g a a t c g g c a a c t t t a g c g a g t a t a g a t g c t g a g c t t c a g a a a c t t a a a c t t a a c t t a g t c c t t a g c c g t t g a a a t c g c t c a t a t c t a c g a c t c g a a g t c t t t g a a t t

2640 EMTNHQKKRAAEMMASLLKG g g a a a t g a c c a a c c a c c a g a a a a a a c g a g c a g c t g a g a t g a t g g c a t c t t t a c t a a a a g g c c t t t a c t g g t t g g t g g t c t t t t t t g c t c g t c g a c t c t a c t a c c g t a g a a a t g a t t t t c c

BsrGI 2700 TAAADRSITRATMTSFVQSG t a c c g c g g c c g c a g a t c g a t c g a t c a c g c g t g c c a c c a t g a c t a g t t t t g t a c a a a g c g g a t g g c g c c g g c g t c t a g c t a g c t a g t g c g c a c g g t g g t a c t g a t c a a a a c a t g t t t c g c c

2760 1 5 10 LLPKNYHLENEVAR GCN4_v1 PGGSGGLLPKNYHLENEVAR t c c g g g a g g t t c t g g a g g a t t g t t g c c c a a a a a c t a c c a c t t g g a g a a c g a g g t c g c a a g a g g c c c t c c a a g a c c t c c t a a c a a c g g g t t t t t g a t g g t g a a c c t c t t g c t c c a g c g t t c

2820 15 20 LKKLVGER (in frame with GCN4_v1) GGSGG 1 5 LLPKNYH GCN4_v1 GCN4_v1 LKKLVGERGGSGGLLPKNYH g t t g a a a a a a t t g g t c g g t g a g c g t g g c g g c t c a g g c g g c c t t c t t c c a a a a a a t t a t c a c a a c t t t t t t a a c c a g c c a c t c g c a c c g c c g a g t c c g c c g g a a g a a g g t t t t t t a a t a g t

2880 10 15 20 LENEVARLKKLVGER GGSGG GCN4_v1 LENEVARLKKLVGERGGSGG t c t t g a a a a t g a a g t t g c t a g a c t t a a a a a a c t t g t t g g a g a a a g a g g c g g g a g c g g c g g a g a a c t t t t a c t t c a a c g a t c t g a a t t t t t t g a a c a a c c t c t t t c t c c g c c c t c g c c g c c

2940 1 5 10 15 20 LLPKNYHLENEVARLKKLVG GCN4_v1 LLPKNYHLENEVARLKKLVG c c t g c t g c c c a a g a a c t a c c a c c t g g a g a a c g a g g t c g c c c g c c t g a a g a a g c t g g t c g g g g a c g a c g g g t t c t t g a t g g t g g a c c t c t t g c t c c a g c g g g c g g a c t t c t t c g a c c a g c c

3000 ER (in frame with GCN4_v1) GGSGG 1 5 10 LLPKNYHLENEVA GCN4_v1 GCN4_v1 ERGGSGGLLPKNYHLENEVA c g a g c g c g g t g g t t c t g g a g g t t t g c t c c c a a a g a a c t a c c a c t t g g a a a a c g a a g t c g c g c t c g c g c c a c c a a g a c c t c c a a a c g a g g g t t t c t t g a t g g t g a a c c t t t t g c t t c a g c g

3060 15 20 RLKKLVGER (in frame with GCN4_v1) GGSGG 1 5 LLPKNY GCN4_v1 GCN4_v1 RLKKLVGERGGSGGLLPKNY a a g a t t g a a g a a g t t g g t c g g t g a a a g a g g a g g a a g c g g a g g a t t a t t a c c t a a a a a t t a t t c t a a c t t c t t c a a c c a g c c a c t t t c t c c t c c t t c g c c t c c t a a t a a t g g a t t t t t a a t

3120 10 15 20 HLENEVARLKKLVGER GGSG GCN4_v1 HLENEVARLKKLVGERGGSG t c a t t t g g a a a a t g a a g t t g c t a g a t t g a a g a a g t t g g t t g g a g a g a g a g g g g g g t c t g g a g t a a a c c t t t t a c t t c a a c g a t c t a a c t t c t t c a a c c a a c c t c t c t c t c c c c c c a g a c c

3180 G 1 5 10 15 LLPKNYHLENEVARLKKLV GCN4_v1 GLLPKNYHLENEVARLKKLV a g g a c t a c t a c c g a a a a a c t a c c a c c t a g a g a a c g a g g t g g c c c g a c t a a a a a a g c t a g t t c c t g a t g a t g g c t t t t t g a t g g t g g a t c t c t t g c t c c a c c g g g c t g a t t t t t t c g a t c a

3240 20 GER (in frame with GCN4_v1) GGSGG 1 5 10 LLPKNYHLENEV GCN4_v1 GCN4_v1 GERGGSGGLLPKNYHLENEV g g g c g a g c g c g g c g g c a g t g g c g g c c t c c t c c c c a a g a a t t a c c a c c t c g a a a a t g a a g t c c c g c t c g c g c c g c c g t c a c c g c c g g a g g a g g g g t t c t t a a t g g t g g a g c t t t t a c t t c a

3300 15 20 ARLKKLVGER (in frame with GCN4_v1) GGSGG 1 5 LLPKN GCN4_v1 GCN4_v1 ARLKKLVGERGGSGGLLPKN a g c a a g g c t c a a g a a g c t c g t a g g a g a a a g a g g t g g t t c c g g g g g a c t c c t t c c a a a a a a t c g t t c c g a g t t c t t c g a g c a t c c t c t t t c t c c a c c a a g g c c c c c t g a g g a a g g t t t t t t

3360 10 15 20 YHLENEVARLKKLVGER GGS GCN4_v1 YHLENEVARLKKLVGERGGS c t a t c a t c t t g a a a a c g a a g t t g c a c g t c t t a a a a a a c t t g t t g g c g a a c g t g g c g g c t c g a t a g t a g a a c t t t t g c t t c a a c g t g c a g a a t t t t t t g a a c a a c c g c t t g c a c c g c c g a g

3420 GG 1 5 10 15 LLPKNYHLENEVARLKKL GCN4_v1 GGLLPKNYHLENEVARLKKL t g g c g g g c t g c t g c c c a a g a a t t a c c a c c t g g a a a a t g a a g t g g c c c g c c t g a a g a a g c t a c c g c c c g a c g a c g g g t t c t t a a t g g t g g a c c t t t t a c t t c a c c g g g c g g a c t t c t t c g a

3480 20 VGER (in frame with GCN4_v1) GGSGG 1 5 10 LLPKNYHLENE GCN4_v1 GCN4_v1 VGERGGSGGLLPKNYHLENE g g t g g g c g a a c g c g g a g g c a g t g g a g g c c t a c t a c c g a a g a a c t a c c a c c t a g a g a a c g a c c a c c c g c t t g c g c c t c c g t c a c c t c c g g a t g a t g g c t t c t t g a t g g t g g a t c t c t t g c t

3540 15 20 VARLKKLVGER (in frame with GCN4_v1) GGSGG 1 LLPK GCN4_v1 GCN4_v1 VARLKKLVGERGGSGGLLPK g g t g g c a c g c c t a a a g a a g c t a g t g g g c g a g c g c g g c g g c t c g g g t g g t t t a t t a c c t a a c c a c c g t g c g g a t t t c t t c g a t c a c c c g c t c g c g c c g c c g a g c c c a c c a a a t a a t g g a t t

3600 5 10 15 20 NYHLENEVARLKKLVGER GG GCN4_v1 NYHLENEVARLKKLVGERGG g a a t t a t c a t t t a g a a a a t g a a g t a g c t c g a t t a a a g a a g t t a g t a g g t g a a c g t g g t g g c t t a a t a g t a a a t c t t t t a c t t c a t c g a g c t a a t t t c t t c a a t c a t c c a c t t g c a c c a c c

3660 SGG 1 5 10 15 LLPKNYHLENEVARLKK GCN4_v1 SGGLLPKNYHLENEVARLKK g t c t g g t g g t c t t c t t c c g a a g a a c t a c c a c c t t g a g a a c g a g g t a g c t c g t c t t a a g a a c a g a c c a c c a g a a g a a g g c t t c t t g a t g g t g g a a c t c t t g c t c c a t c g a g c a g a a t t c t t

3720 20 LVGER (in frame with GCN4_v1) GGSGGGLASHVWCRE GCN4_v1 G G G C T G G C A A G C C A C G T T T G G T G pGEX 5' C C G G G A pGEX 3' LVGERGGSGGGLASHVWCRE a c t t g t a g g t g a g c g t g g t g g a a g c g g t g g t g g g c t g g c a a g c c a c g t t t g g t g c c g g g a t g a a c a t c c a c t c g c a c c a c c t t c g c c a c c a c c c g a c c g t t c g g t g c a a a c c a c g g c c c t

3780 (in frame with GCN4_v1) LHVSEE 1 5 10 LLPKNYHLENEVAR GCN4_v1 G C T G C A T G T G T C A G A G G pGEX 3' LHVSEELLPKNYHLENEVAR g c t g c a t g t g t c a g a g g a a c t t t t g c c a a a g a a t t a t c a t c t t g a g a a c g a a g t g g c t c g c g a c g t a c a c a g t c t c c t t g a a a a c g g t t t c t t a a t a g t a g a a c t c t t g c t t c a c c g a g c

3840 15 20 LKKLVGER (in frame with GCN4_v1) GGSGG 1 5 LLPKNYH GCN4_v1 GCN4_v1 LKKLVGERGGSGGLLPKNYH t c t t a a g a a a c t c g t a g g a g a a a g a g g t g g c a g t g g a g g t c t g c t t c c c a a g a a t t a c c a a g a a t t c t t t g a g c a t c c t c t t t c t c c a c c g t c a c c t c c a g a c g a a g g g t t c t t a a t g g t

3900 10 15 20 LENEVARLKKLVGER GGSGG GCN4_v1 LENEVARLKKLVGERGGSGG c c t g g a a a a t g a g g t a g c t a g a c t g a a a a a g c t c g t a g g c g a g c g c g g g g g a a g t g g g g g g g a c c t t t t a c t c c a t c g a t c t g a c t t t t t c g a g c a t c c g c t c g c g c c c c c t t c a c c c c c

3960 1 5 10 15 20 LLPKNYHLENEVARLKKLVG GCN4_v1 LLPKNYHLENEVARLKKLVG a t t g c t g c c a a a a a a t t a t c a t t t g g a g a a c g a a g t a g c a c g a c t a a a g a a a t t a g t t g g t a a c g a c g g t t t t t t a a t a g t a a a c c t c t t g c t t c a t c g t g c t g a t t t c t t t a a t c a a c c

4020 ER (in frame with GCN4_v1) GGSGG 1 5 10 LLPKNYHLENEVA GCN4_v1 GCN4_v1 ERGGSGGLLPKNYHLENEVA g g a g a g g g g g g g a t c g g g t g g c t t a c t c c c g a a a a a t t a t c a t c t c g a a a a c g a a g t g g c c c t c t c c c c c c c t a g c c c a c c g a a t g a g g g c t t t t t a a t a g t a g a g c t t t t g c t t c a c c g

4080 15 20 RLKKLVGER (in frame with GCN4_v1) GGSGG 1 5 LLPKNY GCN4_v1 GCN4_v1 RLKKLVGERGGSGGLLPKNY t c g g c t a a a a a a g t t a g t t g g a g a g c g t g g c g g t t c t g g a g g c c t a t t a c c a a a a a a c t a a g c c g a t t t t t t c a a t c a a c c t c t c g c a c c g c c a a g a c c t c c g g a t a a t g g t t t t t t g a t

4140 10 15 20 HLENEVARLKKLVGER GGSG GCN4_v1 HLENEVARLKKLVGERGGSG c c a c c t c g a a a a t g a g g t g g c a c g c t t a a a a a a g c t g g t c g g a g a a c g g g g a g g c a g t g g g g t g g a g c t t t t a c t c c a c c g t g c g a a t t t t t t c g a c c a g c c t c t t g c c c c t c c g t c a c c

4200 G 1 5 10 15 LLPKNYHLENEVARLKKLV GCN4_v1 GLLPKNYHLENEVARLKKLV t g g c c t a c t a c c c a a g a a t t a t c a t c t t g a g a a c g a g g t a g c g c g t t t g a a g a a g c t c g t a c c g g a t g a t g g g t t c t t a a t a g t a g a a c t c t t g c t c c a t c g c g c a a a c t t c t t c g a g c a

4260 20 GER (in frame with GCN4_v1) GGSGG 1 5 10 LLPKNYHLENEV GCN4_v1 GCN4_v1 GERGGSGGLLPKNYHLENEV c g g t g a a a g a g g t g g c t c a g g a g g g c t g c t c c c a a a g a a c t a t c a t c t t g a a a a t g a g g t g c c a c t t t c t c c a c c g a g t c c t c c c g a c g a g g g t t t c t t g a t a g t a g a a c t t t t a c t c c a

4320 15 20 ARLKKLVGER (in frame with GCN4_v1) GGSGG 1 5 LLPKN GCN4_v1 GCN4_v1 ARLKKLVGERGGSGGLLPKN c g c t c g a t t a a a a a a g t t a g t g g g c g a g a g g g g a g g c a g t g g t g g g c t a c t t c c t a a g a a g c g a g c t a a t t t t t t c a a t c a c c c g c t c t c c c c t c c g t c a c c a c c c g a t g a a g g a t t c t t

4380 10 15 20 YHLENEVARLKKLVGER GGS GCN4_v1 YHLENEVARLKKLVGERGGS t t a c c a c c t c g a a a a c g a a g t a g c t c g a t t a a a g a a a t t g g t c g g t g a g a g a g g t g g g t c a a t g g t g g a g c t t t t g c t t c a t c g a g c t a a t t t c t t t a a c c a g c c a c t c t c t c c a c c c a g

4440 GG 1 5 10 15 LLPKNYHLENEVARLKKL GCN4_v1 GGLLPKNYHLENEVARLKKL g g g t g g c t t a c t g c c g a a a a a t t a t c a t c t g g a a a a t g a g g t a g c g a g a c t a a a a a a g c t c c c a c c g a a t g a c g g c t t t t t a a t a g t a g a c c t t t t a c t c c a t c g c t c t g a t t t t t t c g a

4500 20 VGER (in frame with GCN4_v1) GGSGG 1 5 10 LLPKNYHLENE GCN4_v1 GCN4_v1 VGERGGSGGLLPKNYHLENE c g t a g g t g a g a g a g g g g g t t c t g g c g g c t t g c t a c c a a a a a a t t a t c a t c t t g a g a a c g a g c a t c c a c t c t c t c c c c c a a g a c c g c c g a a c g a t g g t t t t t t a a t a g t a g a a c t c t t g c t

4560 15 20 VARLKKLVGER (in frame with GCN4_v1) GGSGG 1 LLPK GCN4_v1 GCN4_v1 VARLKKLVGERGGSGGLLPK a g t t g c t a g g c t c a a a a a g c t g g t t g g a g a g c g c g g c g g c t c a g g c g g t c t g t t a c c c a a t c a a c g a t c c g a g t t t t t c g a c c a a c c t c t c g c g c c g c c g a g t c c g c c a g a c a a t g g g t t

4620 5 10 15 20 NYHLENEVARLKKLVGER GG GCN4_v1 NYHLENEVARLKKLVGERGG a a a c t a c c a c t t g g a a a a t g a g g t t g c c a g a t t g a a g a a a c t g g t c g g c g a g c g c g g t g g t t t g a t g g t g a a c c t t t t a c t c c a a c g g t c t a a c t t c t t t g a c c a g c c g c t c g c g c c a c c

4680 SGG 1 5 10 15 LLPKNYHLENEVARLKK GCN4_v1 SGGLLPKNYHLENEVARLKK a a g t g g a g g a c t a c t t c c t a a a a a t t a t c a t t t g g a g a a c g a a g t c g c t a g a c t g a a a a a t t c a c c t c c t g a t g a a g g a t t t t t a a t a g t a a a c c t c t t g c t t c a g c g a t c t g a c t t t t t

BmtINheIEcoRI 4740 20 LVGER (in frame with GCN4_v1) GGSGGKLVEFDT* GCN4_v1 LVGERGGSGGKLVEFDT* g t t g g t t g g g g a a c g a g g a g g g t c t g g c g g c a a g c t a g t c g a a t t c g a c a c g t a g g c t a g c a a c c a a c c c c t t g c t c c t c c c a g a c c g c c g t t c g a t c a g c t t a a g c t g t g c a t c c g a t c

4800 IRES c t t a g t a g g c g g c c g c c c c c c c c c t a a c g t t a c t g g c c g a a g c c g c t t g g a a t a a g g c c g g a a t c a t c c g c c g g c g g g g g g g g g a t t g c a a t g a c c g g c t t c g g c g a a c c t t a t t c c g g c

4860 IRES g t g t g c g t t t g t c t a t a t g t t a t t t t c c a c c a t a t t g c c g t c t t t t g g c a a t g t g a g g g c c a c a c g c a a a c a g a t a t a c a a t a a a a g g t g g t a t a a c g g c a g a a a a c c g t t a c a c t c c c g

4920 IRES G A G A G C G G T T IRES reverse c c g g a a a c c t g g c c c t g t c t t c t t g a c g a g c a t t c c t a g g g g t c t t t c c c c t c t c g c c a a g g c c t t t g g a c c g g g a c a g a a g a a c t g c t c g t a a g g a t c c c c a g a a a g g g g a g a g c g g t t

4980 IRES T C C T T A C G IRES reverse a g g a a t g c a a g g t c t g t t g a a t g t c g t g a a g g a a g c a g t t c c t c t g g a a g c t t c t t g a a g t c c t t a c g t t c c a g a c a a c t t a c a g c a c t t c c t t c g t c a a g g a g a c c t t c g a a g a a c t t c

5040 IRES a c a a a c a a c g t c t g t a g c g a c c c t t t g c a g g c a g c g g a a c c c c c c a c c t g g c g a c a g g t g t g t t t g t t g c a g a c a t c g c t g g g a a a c g t c c g t c g c c t t g g g g g g t g g a c c g c t g t c c a c

BspMIPaqCIBfuAI 5100 IRES c c t c t g c g g c c a a a a g c c a c g t g t a t a a g a t a c a c c t g c a a a g g c g g c a c a a c c c c a g t g g g a g a c g c c g g t t t t c g g t g c a c a t a t t c t a t g t g g a c g t t t c c g c c g t g t t g g g g t c a c

5160 IRES T G G C T C T C C T C A A G C G T A T T IRES-F c c a c g t t g t g a g t t g g a t a g t t g t g g a a a g a g t c a a a t g g c t c t c c t c a a g c g t a t t c a a g g t g c a a c a c t c a a c c t a t c a a c a c c t t t c t c a g t t t a c c g a g a g g a g t t c g c a t a a g t t

PflMI 5220 IRES c a a g g g g c t g a a g g a t g c c c a g a a g g t a c c c c a t t g t a t g g g a t c t g a t c t g g g g c c t c g g t t c c c c g a c t t c c t a c g g g t c t t c c a t g g g g t a a c a t a c c c t a g a c t a g a c c c c g g a g c

5280 IRES g t g c a c a t g c t t t a c a t g t g t t t a g t c g a g g t t a a a a a a a c g t c t a g g c c c c c c g a a c c a c a c g t g t a c g a a a t g t a c a c a a a t c a g c t c c a a t t t t t t t g c a g a t c c g g g g g g c t t g g t

5340 1 MTE IRES PuroR T A C T G G C T C A Puro-R MATTMTE c g g g g a c g t g g t t t t c c t t t g a a a a a c a c g a t g a t a a t a t g g c c a c a a c c a t g a c c g a g t g c c c c t g c a c c a a a a g g a a a c t t t t t g t g c t a c t a t t a t a c c g g t g t t g g t a c t g g c t c a

BsiWI 5400 5 10 15 20 YKPTVRLATRDDVPRAVRTL PuroR T G T T C G G G T G Puro-R YKPTVRLATRDDVPRAVRTL a c a a g c c c a c g g t g c g c c t c g c c a c c c g c g a c g a c g t c c c c c g g g c c g t a c g c a c c c t c g t g t t c g g g t g c c a c g c g g a g c g g t g g g c g c t g c t g c a g g g g g c c c g g c a t g c g t g g g a g c

5460 25 30 35 40 AAAFADYPATRHTVDPDRHI PuroR AAAFADYPATRHTVDPDRHI c c g c c g c g t t c g c c g a c t a c c c c g c c a c g c g c c a c a c c g t c g a c c c g g a c c g c c a c a t c g g g c g g c g c a a g c g g c t g a t g g g g c g g t g c g c g g t g t g g c a g c t g g g c c t g g c g g t g t a g c

BstEII 5520 45 50 55 60 ERVTELQELFLTRVGLDIGK PuroR ERVTELQELFLTRVGLDIGK a g c g g g t c a c c g a g c t g c a a g a a c t c t t c c t c a c g c g c g t c g g g c t c g a c a t c g g c a a g g t c g c c c a g t g g c t c g a c g t t c t t g a g a a g g a g t g c g c g c a g c c c g a g c t g t a g c c g t t c c

5580 65 70 75 80 VWVADDGAAVAVWTTPESVE PuroR VWVADDGAAVAVWTTPESVE t g t g g g t c g c g g a c g a c g g c g c c g c g g t g g c g g t c t g g a c c a c g c c g g a g a g c g t c g a a g a c a c c c a g c g c c t g c t g c c g c g g c g c c a c c g c c a g a c c t g g t g c g g c c t c t c g c a g c t t c

5640 85 90 95 100 AGAVFAEIGPRMAELSGSRL PuroR AGAVFAEIGPRMAELSGSRL c g g g g g c g g t g t t c g c c g a g a t c g g c c c g c g c a t g g c c g a g t t g a g c g g t t c c c g g c t g g g c c c c c g c c a c a a g c g g c t c t a g c c g g g c g c g t a c c g g c t c a a c t c g c c a a g g g c c g a c c

5700 105 110 115 120 AAQQQMEGLLAPHRPKEPAW PuroR AAQQQMEGLLAPHRPKEPAW c c g c g c a g c a a c a g a t g g a a g g c c t c c t g g c g c c g c a c c g g c c c a a g g a g c c c g c g t g g t g g c g c g t c g t t g t c t a c c t t c c g g a g g a c c g c g g c g t g g c c g g g t t c c t c g g g c g c a c c a

5760 125 130 135 140 FLATVGVSPDHQGKGLGSAV PuroR FLATVGVSPDHQGKGLGSAV t c c t g g c c a c c g t c g g c g t c t c g c c c g a c c a c c a g g g c a a g g g t c t g g g c a g c g c c g t c g a g g a c c g g t g g c a g c c g c a g a g c g g g c t g g t g g t c c c g t t c c c a g a c c c g t c g c g g c a g c

5820 145 150 155 160 VLPGVEAAERAGVPAFLETS PuroR VLPGVEAAERAGVPAFLETS t g c t c c c c g g a g t g g a g g c g g c c g a g c g c g c c g g g g t g c c c g c c t t c c t g g a g a c c t c c g a c g a g g g g c c t c a c c t c c g c c g g c t c g c g c g g c c c c a c g g g c g g a a g g a c c t c t g g a g g c

5880 165 170 175 180 APRNLPFYERLGFTVTADVE PuroR G C A A C C T C C C C T T C T A C G A G C Puro-F APRNLPFYERLGFTVTADVE c g c c c c g c a a c c t c c c c t t c t a c g a g c g g c t c g g c t t c a c c g t c a c c g c c g a c g t c g a g g g c g g g g c g t t g g a g g g g a a g a t g c t c g c c g a g c c g a a g t g g c a g t g g c g g c t g c a g c t c c

PaeR7IPspXIXhoIPshAI 5940 185 190 195 200 VPEGPRTWCMTRKPGA* PuroR VPEGPRTWCMTRKPGA* t g c c c g a a g g a c c g c g c a c c t g g t g c a t g a c c c g c a a g c c c g g t g c c t g a c t c g a g t c t a a c g g g c t t c c t g g c g c g t g g a c c a c g t a c t g g g c g t t c g g g c c a c g g a c t g a g c t c a g a t

6000 bGH poly(A) signal G G A G C T G A C A C G G A A G A T BGH-rev g a g g g c c c g t t t a a a c c c g c t g a t c a g c c t c g a c t g t g c c t t c t a g t t g c c a g c c a t c t g c t c c c g g g c a a a t t t g g g c g a c t a g t c g g a g c t g a c a c g g a a g a t c a a c g g t c g g t a g a c

6060 bGH poly(A) signal t t g t t t g c c c c t c c c c c g t g c c t t c c t t g a c c c t g g a a g g t g c c a c t c c c a c t g t c c t t t a a c a a a c g g g g a g g g g g c a c g g a a g g a a c t g g g a c c t t c c a c g g t g a g g g t g a c a g g a a a

6120 bGH poly(A) signal c c t a a t a a a a t g a g g a a a t t g c a t c g c a t t g t c t g a g t a g g t g t c a t t c t a t t c t g g g g g g g a t t a t t t t a c t c c t t t a a c g t a g c g t a a c a g a c t c a t c c a c a g t a a g a t a a g a c c c c c

6180 bGH poly(A) signal g t g g g g t g g g g c a g g a c a g c a a g g g g g a g g a t t g g g a a g a c a a t a g c a g g c a t g c t g g g g c a c c c c a c c c c g t c c t g t c g t t c c c c c t c c t a a c c c t t c t g t t a t c g t c c g t a c g a c c c c

6240 bGH poly(A) signal a t g c g g t g g g c t c t a t g g c t t c t g a g g c g g a a a g a a c c a g c t g g g g c t c t a g g g g g t a t c t a c g c c a c c c g a g a t a c c g a a g a c t c c g c c t t t c t t g g t c g a c c c c g a g a t c c c c c a t a g

6300 f1 ori c c c a c g c g c c c t g t a g c g g c g c a t t a a g c g c g g c g g g t g t g g t g g t t a c g c g c a g c g t g a g g g t g c g c g g g a c a t c g c c g c g t a a t t c g c g c c g c c c a c a c c a c c a a t g c g c g t c g c a c t

6360 f1 ori G A G G A A A G C G A A A G A A G G G A F1ori-R c c g c t a c a c t t g c c a g c g c c c t a g c g c c c g c t c c t t t c g c t t t c t t c c c t t c c t t t c t c g g g c g a t g t g a a c g g t c g c g g g a t c g c g g g c g a g g a a a g c g a a a g a a g g g a a g g a a a g a g c

6420 f1 ori c c a c g t t c g c c g g c t t t c c c c g t c a a g c t c t a a a t c g g g g g c t c c c t t t a g g g t t c c g a t g g t g c a a g c g g c c g a a a g g g g c a g t t c g a g a t t t a g c c c c c g a g g g a a a t c c c a a g g c t a

6480 f1 ori t t a g t g c t t t a c g g c a c c t c g a c c c c a a a a a a c t t g a t t a g g g t g a t g g t t c a c g t a g t g a a t c a c g a a a t g c c g t g g a g c t g g g g t t t t t t g a a c t a a t c c c a c t a c c a a g t g c a t c a c

6540 f1 ori g g c c a t c g c c c t g a t a g a c g g t t t t t c g c c c t t t g a c g t t g g a g t c c a c g t t c t t t a a t a c c g g t a g c g g g a c t a t c t g c c a a a a a g c g g g a a a c t g c a a c c t c a g g t g c a a g a a a t t a t

6600 f1 ori G T G G A C T C T T G T T C C A A A C T G G F1ori-F g t g g a c t c t t g t t c c a a a c t g g a a c a a c a c t c a a c c c t a t c t c g g t c t a t t c t t t t g a t t c a c c t g a g a a c a a g g t t t g a c c t t g t t g t g a g t t g g g a t a g a g c c a g a t a a g a a a a c t a a

6660 f1 ori t a t a a g g g a t t t t g c c g a t t t c g g c c t a t t g g t t a a a a a a t g a g c t g a t t t a a c a a a a a t a t a t t c c c t a a a a c g g c t a a a g c c g g a t a a c c a a t t t t t t a c t c g a c t a a a t t g t t t t t a

6720 f1 ori SV40 promoter C C T T A C A C A C A G T C A A T C C C A pBABE 3' t t a a c g c g a a t t a a t t c t g t g g a a t g t g t g t c a g t t a g g g t g t g g a a a g t c c c c a g g c t c a a t t g c g c t t a a t t a a g a c a c c t t a c a c a c a g t c a a t c c c a c a c c t t t c a g g g g t c c g a g

6780 SV40 promoter c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a g g t g t g g a a a g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t c c a c a c c t t t

6840 SV40 promoter g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g

6900 SV40 promoter SV40 ori c a t a g t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c c a g t t c c g c c c a t t c g t a t c a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g g t c a a g g c g g g t a a g

6960 SV40 promoter SV40 ori T A T T T A T G C A G A G G C C G A G G SV40pro-F t c c g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c c g c c t c t g c c t c a g g c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c g g c g g a g a c g g a g

7020 SV40 promoter SV40 ori t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g c t t t t g c a a a a a g c t a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c g a a a a c g t t t t t c g a

7080 EM7 promoter c c c g g g a g c t t g t a t a t c c a t t t t c g g a t c t g a t c a g c a c g t g t t g a c a a t t a a t c a t c g g g g c c c t c g a a c a t a t a g g t a a a a g c c t a g a c t a g t c g t g c a c a a c t g t t a a t t a g t a g c

7140 1 MAKL EM7 promoter BleoR g c a t a g t a t a t c g g c a t a g t a t a a t a c g a c a a g g t g a g g a a c t a a a c c a t g g c c a a g t t g c g t a t c a t a t a g c c g t a t c a t a t t a t g c t g t t c c a c t c c t t g a t t t g g t a c c g g t t c a a c

MauBI 7200 5 10 15 20 TSAVPVLTARDVAGAVEFWT BleoR a c c a g t g c c g t t c c g g t g c t c a c c g c g c g c g a c g t c g c c g g a g c g g t c g a g t t c t g g a c c t g g t c a c g g c a a g g c c a c g a g t g g c g c g c g c t g c a g c g g c c t c g c c a g c t c a a g a c c t g g

SgrAI 7260 25 30 35 40 DRLGFSRDFVEDDFAGVVRD BleoR g a c c g g c t c g g g t t c t c c c g g g a c t t c g t g g a g g a c g a c t t c g c c g g t g t g g t c c g g g a c c t g g c c g a g c c c a a g a g g g c c c t g a a g c a c c t c c t g c t g a a g c g g c c a c a c c a g g c c c t g

7320 45 50 55 60 DVTLFISAVQDQVVPDNTLA BleoR g a c g t g a c c c t g t t c a t c a g c g c g g t c c a g g a c c a g g t g g t g c c g g a c a a c a c c c t g g c c c t g c a c t g g g a c a a g t a g t c g c g c c a g g t c c t g g t c c a c c a c g g c c t g t t g t g g g a c c g g

7380 65 70 75 80 WVWVRGLDELYAEWSEVVST BleoR t g g g t g t g g g t g c g c g g c c t g g a c g a g c t g t a c g c c g a g t g g t c g g a g g t c g t g t c c a c g a c c c a c a c c c a c g c g c c g g a c c t g c t c g a c a t g c g g c t c a c c a g c c t c c a g c a c a g g t g c

FseI 7440 85 90 95 100 NFRDASGPAMTEIGEQPWGR BleoR a a c t t c c g g g a c g c c t c c g g g c c g g c c a t g a c c g a g a t c g g c g a g c a g c c g t g g g g g c g g t t g a a g g c c c t g c g g a g g c c c g g c c g g t a c t g g c t c t a g c c g c t c g t c g g c a c c c c c g c c

7500 105 110 115 120 EFALRDPAGNCVHFVAEEQD BleoR g a g t t c g c c c t g c g c g a c c c g g c c g g c a a c t g c g t g c a c t t c g t g g c c g a g g a g c a g g a c c t c a a g c g g g a c g c g c t g g g c c g g c c g t t g a c g c a c g t g a a g c a c c g g c t c c t c g t c c t g

7560 125 * BleoR t g a c a c g t g c t a c g a g a t t t c g a t t c c a c c g c c g c c t t c t a t g a a a g g t t g g g c t t c g g a a c t g t g c a c g a t g c t c t a a a g c t a a g g t g g c g g c g g a a g a t a c t t t c c a a c c c g a a g c c t

7620 a t c g t t t t c c g g g a c g c c g g c t g g a t g a t c c t c c a g c g c g g g g a t c t c a t g c t g g a g t t c t a g c a a a a g g c c c t g c g g c c g a c c t a c t a g g a g g t c g c g c c c c t a g a g t a c g a c c t c a a g

7680 SV40 poly(A) signal C G T T A T C G T A G SV40pA-R t t c g c c c a c c c c a a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c a a t a g c a t c a a g c g g g t g g g g t t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g t t a t c g t a g

7740 SV40 poly(A) signal G T G G T T T G T C C A A A C T C EBV-rev T G T T T A A A G SV40pA-R a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t c c a a a c t c t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c a g g t t t g a g

BstZ17I 7800 SV40 poly(A) signal A T C EBV-rev a t c a a t g t a t c t t a t c a t g t c t g t a t a c c g t c g a c c t c t a g c t a g a g c t t g g c g t a a t c a t a g t t a c a t a g a a t a g t a c a g a c a t a t g g c a g c t g g a g a t c g a t c t c g a a c c g c a t t a g t

7860 -10 M13 rev lac operator lac promoter C A G T A T C G A C A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse t g g t c a t a g c t g t t t c c t g t g t g a a a t t g t t a t c c g c t c a c a a t t c c a c a c a a c a t a c g a a c c a g t a t c g a c a a a g g a c a c a c t t t a a c a a t a g g c g a g t g t t a a g g t g t g t t g t a t g c t

7920 -35 lac promoter CAP binding site g c c g g a a g c a t a a a g t g t a a a g c c t g g g g t g c c t a a t g a g t g a g c t a a c t c a c a t t a a t t c g g c c t t c g t a t t t c a c a t t t c g g a c c c c a c g g a t t a c t c a c t c g a t t g a g t g t a a t t a a

7980 g c g t t g c g c t c a c t g c c c g c t t t c c a g t c g g g a a a c c t g t c g t g c c a g c t g c a t t a a t g a c g c a a c g c g a g t g a c g g g c g a a a g g t c a g c c c t t t g g a c a g c a c g g t c g a c g t a a t t a c t

BspQISapI 8040 G A G C G A G L4440 a t c g g c c a a c g c g c g g g g a g a g g c g g t t t g c g t a t t g g g c g c t c t t c c g c t t c c t c g c t c t a g c c g g t t g c g c g c c c c t c t c c g c c a a a c g c a t a a c c c g c g a g a a g g c g a a g g a g c g a g

8100 T G A C T G A G C G A L4440 a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a a g g c g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t t c c g c

8160 g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a a a g g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t t t c c g

8220 ori c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t c c g c g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a g g c g

8280 ori c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g g a g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t

8340 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g

8400 ori c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a t g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t a

8460 ori a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c

8520 ori c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g g

8580 ori a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g a t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c g t c t

8640 ori g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a c a c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t g t g a

8700 ori a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g a g t t t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c t c a a

8760 ori g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t t t t t t t g t t t g c a a g c a g c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a a a a a a a c a a a c g t t c g t c

8820 ori c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g g g g t c t g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g c c c c a g a

8880 g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t c a a a a a g g c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a g t t t t t c c

8940 a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t c t a a a g t a t a t a t t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t t t c a t a t a t a

9000 285 280 275 *WHKILSAGIEAI AmpR *WHKILSAGIEAI g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t t a a t c a g t g a g g c a c c t a t c t c a g c g a t c c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a a t t a g t c a c t c c g t g g a t a g a g t c g c t a g

AhdI 9060 270 265 260 255 QRNREDMTAQSGTTYIVVIR AmpR QRNREDMTAQSGTTYIVVIR t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c t c c c c g t c g t g t a g a t a a c t a c g a t a c g g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g g g g c a g c a c a t c t a t t g a t g c t a t g c c

9120 250 245 240 235 SPKGDPGLAAIIGRSGREGA AmpR SPKGDPGLAAIIGRSGREGA g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c g a g a c c c a c g c t c a c c g g c t c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t a c t a t g g c g c t c t g g g t g c g a g t g g c c g a

9180 230 225 220 215 GSKDAIFWGAPLASRLLPGA AmpR GSKDAIFWGAPLASRLLPGA c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g a g c g c a g a a g t g g t c c t g c a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t c c c g g c t c g c g t c t t c a c c a g g a c g t

9240 210 205 200 195 VKDAEMWDILQQRSALTLLE AmpR VKDAEMWDILQQRSALTLLE a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g g a a g c t a g a g t a a g t a g t t c g t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c a a c g g c c c t t c g a t c t c a t t c a t c a a g c

FspI 9300 190 185 180 175 GTLLKRLTTAMAVPMTTDRE AmpR GTLLKRLTTAMAVPMTTDRE c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a t t g c t a c a g g c a t c g t g g t g t c a c g c t c g g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t a a c g a t g t c c g t a g c a c c a c a g t g c g a g c

9360 170 165 160 155 DNPIAENLEPEWRDLRTVHD AmpR DNPIAENLEPEWRDLRTVHD t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c a a c g a t c a a g g c g a g t t a c a t g a t c c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g g t t g c t a g t t c c g c t c a a t g t a c t a g g

9420 150 145 140 135 GMNHLFATLEKPGGITTLLL AmpR GMNHLFATLEKPGGITTLLL c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t t c g g t c c t c c g a t c g t t g t c a g a a g t a a g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g c t a g c a a c a g t c t t c a t t c

9480 130 125 120 115 NAATNDSMTIAASCLERVTM AmpR NAATNDSMTIAASCLERVTM t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a c t g c a t a a t t c t c t t a c t g t c a t g a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c g t c g t g a c g t a t t a a g a g a a t g a c a g t a c

9540 110 105 100 95 GDTLHKETVPSYEVLDNQSY AmpR GDTLHKETVPSYEVLDNQSY c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g a g t a c t c a a c c a a g t c a t t c t g a g a a t a g g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c a t g a g t t g g t t c a g t a a g a c t c t t a t c

9600 90 85 80 75 HIRRGLQEQGADIRSLVAGC AmpR A T A A T A C C G C G C C A C A T Amp-R HIRRGLQEQGADIRSLVAGC t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g c g t c a a t a c g g g a t a a t a c c g c g c c a c a t a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c g c a g t t a t g c c c t a t t a t g g c g c g g t g t a

9660 70 65 60 55 LLVKFTSMMPFREEPRFSEL AmpR A G C Amp-R LLVKFTSMMPFREEPRFSEL a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t c t t c g g g g c g a a a a c t c t c a a g g t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t t t g c a a g a a g c c c c g c t t t t g a g a g t t c c

9720 50 45 40 35 IKGSNLDLEIYGVRAGLQDE AmpR IKGSNLDLEIYGVRAGLQDE a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t a a c c c a c t c g t g c a c c c a a c t g a t c t t c a t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c a t t g g g t g a g c a c g t g g g t t g a c t a g a a g t

9780 30 25 ADKVKVLTEPH 20 15 signal sequence AFVPLCFAA AmpR ADKVKVLTEPHAFVPLCFAA g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t g a g c a a a a a c a g g a a g g c a a a a t g c c g c a c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a c t c g t t t t t g t c c t t c c g t t t t a c g g c g t

9840 10 5 1 signal sequence FFPILAVRFHQISM AmpR AmpR promoter FFPILAVRFHQISM a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t t g a a t a c t c a t a c t c t t c c t t t t t c a a t a t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a a c t t a t g a g t a t g a g a a g g a a a a a g t t a t a

9900 AmpR promoter t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a t g t a t t t a g a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a a c t t a c a t a a a t c

3ʹ 5ʹ 9957 AmpR promoter a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c c t g a c g t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g t g g a c t g c a g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.