Addgene DYSF-Exon36-del - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: DYSF-Exon36-del


Map View

pBRforEco (8617 .. 8635) NruI (8355) Kan-R (8352 .. 8371) AsiSI - PvuI (8014) pBR322ori-F (6950 .. 6969) L4440 (6699 .. 6716) BspQI - SapI (6693) CAP binding site M13/pUC Reverse (6481 .. 6503) lac operator M13 Reverse (6468 .. 6484) M13 rev NotI (6457) BmtI (6454) NheI (6450) NdeI (6445) BclI * (6432) HindIII (6228) SphI (5984) BsrDI (5842) BsaAI - PmlI (5480) StuI (4728) DraIII (4512) pGEX 3' (29 .. 51) pRS-marker (151 .. 170) EcoRI (183) EcoRV (191) HpaI (197) Acc65I (201) KpnI (205) SpeI (207) MluI (213) SacII (295) XbaI (843) BstBI (1725) BsaI (1893) Bsu36I (1977) ScaI (1992) PflFI - Tth111I (2595) AleI (3138) PaeR7I - PspXI - XhoI (3459) AfeI (4171) DYSF-Exon36-del 8644 bp

Sequence View

5ʹ 3ʹ 60 G G A G A C T G T G T A C G T C G A G G G C C pGEX 3' t c g c g c g t t t c g g t g a t g a c g g t g a a a a c c t c t g a c a c a t g c a g c t c c c g g a g a c g g t c a a g c g c g c a a a g c c a c t a c t g c c a c t t t t g g a g a c t g t g t a c g t c g a g g g c c t c t g c c a g t

120 c a g c t t g t c t g t a a g c g g a t g c c g g g a g c a g a c a a g c c c g t c a g g g c g c g t c a g c g g g t g g t c g a a c a g a c a t t c g c c t a c g g c c c t c g t c t g t t c g g g c a g t c c c g c g c a g t c g c c c a c

180 C G G C A T C A G A G C A G A T T G T A pRS-marker t t g g c g g g t g t c g g g g c t g g c t t a a c t a t g c g g c a t c a g a g c a g a t t g t a c t g a g a g t g c a a c c g c c c a c a g c c c c g a c c g a a t t g a t a c g c c g t a g t c t c g t c t a a c a t g a c t c t c a c g

MluIEcoRIEcoRVHpaIAcc65IKpnISpeI 240 MLRVFIL a c g a a t t c g a t a t c g t t a a c g g t a c c a c t a g t a c g c g t a t g c t g a g g g t c t t c a t c c t c t t g c t t a a g c t a t a g c a a t t g c c a t g g t g a t c a t g c g c a t a c g a c t c c c a g a a g t a g g a g a

SacII 300 YAENVHTPDTDISDAYCSAV a t g c c g a g a a c g t c c a c a c a c c c g a c a c c g a c a t c a g c g a t g c c t a c t g c t c c g c g g t g t t a c g g c t c t t g c a g g t g t g t g g g c t g t g g c t g t a g t c g c t a c g g a t g a c g a g g c g c c a c a

360 FAGVKKRTKVIKNSVNPVWN t t g c a g g g g t g a a g a a g a g a a c c a a a g t c a t c a a g a a c a g c g t g a a c c c t g t a t g g a a t g a a c g t c c c c a c t t c t t c t c t t g g t t t c a g t a g t t c t t g t c g c a c t t g g g a c a t a c c t t a c

420 EGFEWDLKGIPLDQGSELHV a g g g a t t t g a a t g g g a c c t c a a g g g c a t c c c c c t g g a c c a g g g c t c t g a g c t t c a t g t g g t c c c t a a a c t t a c c c t g g a g t t c c c g t a g g g g g a c c t g g t c c c g a g a c t c g a a g t a c a c c

480 VVKDHETMGRNRFLGEAKVP t g g t c a a a g a c c a t g a g a c g a t g g g g a g g a a c a g g t t c c t g g g g g a a g c c a a g g t c c c a c a c c a g t t t c t g g t a c t c t g c t a c c c c t c c t t g t c c a a g g a c c c c c t t c g g t t c c a g g g t g

540 LREVLATPSLSASFNAPLLD t c c g a g a g g t c c t c g c c a c c c c t a g t c t g t c c g c c a g c t t c a a t g c c c c c c t g c t g g a c a a g g c t c t c c a g g a g c g g t g g g g a t c a g a c a g g c g g t c g a a g t t a c g g g g g g a c g a c c t g t

600 TKKQPTGASLVLQVSYTPLP c c a a g a a g c a g c c c a c a g g g g c c t c g c t g g t c c t g c a g g t g t c c t a c a c a c c g c t g c c t g g g t t c t t c g t c g g g t g t c c c c g g a g c g a c c a g g a c g t c c a c a g g a t g t g t g g c g a c g g a c

660 GAVPLFPPPTPLEPSPTLPD g a g c t g t g c c c c t g t t c c c g c c c c c t a c t c c t c t g g a g c c c t c c c c g a c t c t g c c t g a c c c t c g a c a c g g g g a c a a g g g c g g g g g a t g a g g a g a c c t c g g g a g g g g c t g a g a c g g a c t g g

720 LDVVADTGGEEDTEDQGLTG t g g a t g t a g t g g c a g a c a c a g g a g g a g a g g a a g a c a c a g a g g a c c a g g g a c t c a c t g g a g a c c t a c a t c a c c g t c t g t g t c c t c c t c t c c t t c t g t g t c t c c t g g t c c c t g a g t g a c c t c

780 DEAEPFLDQSGGPGAPTTPR a t g a g g c g g a g c c a t t c c t g g a t c a a a g c g g a g g c c c g g g g g c t c c c a c c a c c c c a a g g a t a c t c c g c c t c g g t a a g g a c c t a g t t t c g c c t c c g g g c c c c c g a g g g t g g t g g g g t t c c t

840 KLPSRPPPHYPGIKRKRSAP a a c t a c c t t c a c g t c c t c c g c c c c a c t a c c c c g g g a t c a a a a g a a a g c g a a g t g c g c c t a t t g a t g g a a g t g c a g g a g g c g g g g t g a t g g g g c c c t a g t t t t c t t t c g c t t c a c g c g g a t

XbaI 900 1 I Factor Xa site TSRKLLSDKPQDFQIRVQVI c a t c t a g a a a g c t g c t g t c a g a c a a a c c g c a g g a t t t c c a g a t c a g g g t c c a g g t g a t c g g t a g a t c t t t c g a c g a c a g t c t g t t t g g c g t c c t a a a g g t c t a g t c c c a g g t c c a c t a g c

960 EGR (in frame with Factor Xa site) QLPGVNIKPVVKVTAAG Factor Xa site EGRQLPGVNIKPVVKVTAAG a g g g g c g c c a g c t g c c g g g g g t g a a c a t c a a g c c t g t g g t c a a g g t t a c c g c t g c a g g g c t c c c c g c g g t c g a c g g c c c c c a c t t g t a g t t c g g a c a c c a g t t c c a a t g g c g a c g t c c c g

1020 (in frame with Factor Xa site) QTKRTRIHKGNSPLFNETLF QTKRTRIHKGNSPLFNETLF a g a c c a a g c g g a c g c g g a t c c a c a a g g g a a a c a g c c c a c t c t t c a a t g a g a c t c t t t t c t t c t g g t t c g c c t g c g c c t a g g t g t t c c c t t t g t c g g g t g a g a a g t t a c t c t g a g a a a a g a

1080 (in frame with Factor Xa site) FNLFDSPGELFDEPIFITVV FNLFDSPGELFDEPIFITVV t c a a c t t g t t t g a c t c t c c t g g g g a g c t g t t t g a t g a g c c c a t c t t t a t c a c g g t g g t a g a g t t g a a c a a a c t g a g a g g a c c c c t c g a c a a a c t a c t c g g g t a g a a a t a g t g c c a c c a t c

1140 (in frame with Factor Xa site) DSRSLRTDALLGEFRMDVGT DSRSLRTDALLGEFRMDVGT a c t c t c g t t c t c t c a g g a c a g a t g c t c t c c t c g g g g a g t t c c g g a t g g a c g t g g g c a c c a t g a g a g c a a g a g a g t c c t g t c t a c g a g a g g a g c c c c t c a a g g c c t a c c t g c a c c c g t g g t

1200 (in frame with Factor Xa site) IYREPRHAYLRKWLLLSDPD IYREPRHAYLRKWLLLSDPD t t t a c a g a g a g c c c c g g c a c g c c t a t c t c a g g a a g t g g c t g c t g c t c t c a g a c c c t g a t g a a a t g t c t c t c g g g g c c g t g c g g a t a g a g t c c t t c a c c g a c g a c g a g a g t c t g g g a c t a c

1260 (in frame with Factor Xa site) DFSAGARGYLKTSLCVLGPG DFSAGARGYLKTSLCVLGPG a c t t c t c t g c t g g g g c c a g a g g c t a c c t g a a a a c a a g c c t t t g t g t g c t g g g g c c t g g g g t g a a g a g a c g a c c c c g g t c t c c g a t g g a c t t t t g t t c g g a a a c a c a c g a c c c c g g a c c c c

1320 (in frame with Factor Xa site) DEAPLERKDPSEDKEDIESN DEAPLERKDPSEDKEDIESN a c g a a g c g c c t c t g g a g a g a a a a g a c c c c t c t g a a g a c a a g g a g g a c a t t g a a a g c a a c c t g c t t c g c g g a g a c c t c t c t t t t c t g g g g a g a c t t c t g t t c c t c c t g t a a c t t t c g t t g g

1380 (in frame with Factor Xa site) LLRPTGVALRGAHFCLKVFR LLRPTGVALRGAHFCLKVFR t g c t c c g g c c c a c a g g c g t a g c c c t g c g a g g a g c c c a c t t c t g c c t g a a g g t c t t c c g g g a c g a g g c c g g g t g t c c g c a t c g g g a c g c t c c t c g g g t g a a g a c g g a c t t c c a g a a g g c c c

1440 (in frame with Factor Xa site) AEDLPQMDDAVMDNVKQIFG AEDLPQMDDAVMDNVKQIFG c c g a g g a c t t g c c g c a g a t g g a c g a t g c c g t g a t g g a c a a c g t g a a a c a g a t c t t t g g c t g g c t c c t g a a c g g c g t c t a c c t g c t a c g g c a c t a c c t g t t g c a c t t t g t c t a g a a a c c g a

1500 (in frame with Factor Xa site) FESNKKNLVDPFVEVSFAGK FESNKKNLVDPFVEVSFAGK t c g a g a g t a a c a a g a a g a a c t t g g t g g a c c c c t t t g t g g a g g t c a g c t t t g c g g g g a a a a a g c t c t c a t t g t t c t t c t t g a a c c a c c t g g g g a a a c a c c t c c a g t c g a a a c g c c c c t t t t

1560 (in frame with Factor Xa site) MLCSKILEKTANPQWNQNIT MLCSKILEKTANPQWNQNIT t g c t g t g c a g c a a g a t c t t g g a g a a g a c g g c c a a c c c t c a g t g g a a c c a g a a c a t c a c a c a c g a c a c g t c g t t c t a g a a c c t c t t c t g c c g g t t g g g a g t c a c c t t g g t c t t g t a g t g t g

1620 (in frame with Factor Xa site) LPAMFPSMCEKMRIRIIDWD LPAMFPSMCEKMRIRIIDWD t g c c t g c c a t g t t t c c c t c c a t g t g c g a a a a a a t g a g g a t t c g t a t c a t a g a c t g g g a c c a c g g a c g g t a c a a a g g g a g g t a c a c g c t t t t t t a c t c c t a a g c a t a g t a t c t g a c c c t g g

1680 (in frame with Factor Xa site) RLTHNDIVATTYLSMSKISA RLTHNDIVATTYLSMSKISA g c c t g a c t c a c a a t g a c a t c g t g g c t a c c a c c t a c c t g a g t a t g t c g a a a a t c t c t g c c c c g g a c t g a g t g t t a c t g t a g c a c c g a t g g t g g a t g g a c t c a t a c a g c t t t t a g a g a c g g g

BstBI 1740 (in frame with Factor Xa site) PGGEIEEEPAGAVKPSKASD PGGEIEEEPAGAVKPSKASD c t g g a g g a g a a a t a g a a g a g g a g c c t g c a g g t g c t g t c a a g c c t t c g a a a g c c t c a g a c t g a c c t c c t c t t t a t c t t c t c c t c g g a c g t c c a c g a c a g t t c g g a a g c t t t c g g a g t c t g a

1800 (in frame with Factor Xa site) LDDYLGFLPTFGPCYINLYG LDDYLGFLPTFGPCYINLYG t g g a t g a c t a c c t g g g c t t c c t c c c c a c t t t t g g g c c c t g c t a c a t c a a c c t c t a t g g c a a c c t a c t g a t g g a c c c g a a g g a g g g g t g a a a a c c c g g g a c g a t g t a g t t g g a g a t a c c g t

1860 (in frame with Factor Xa site) SPREFTGFPDPYTELNTGKG SPREFTGFPDPYTELNTGKG g t c c c a g a g a g t t c a c a g g c t t c c c a g a c c c c t a c a c a g a g c t c a a c a c a g g c a a g g g g g c a g g g t c t c t c a a g t g t c c g a a g g g t c t g g g g a t g t g t c t c g a g t t g t g t c c g t t c c c c c

BsaI 1920 (in frame with Factor Xa site) EGVAYRGRLLLSLETKLVEH EGVAYRGRLLLSLETKLVEH a a g g t g t g g c t t a t c g t g g c c g g c t t c t g c t c t c c c t g g a g a c c a a g c t g g t g g a g c a c a t t c c a c a c c g a a t a g c a c c g g c c g a a g a c g a g a g g g a c c t c t g g t t c g a c c a c c t c g t g t

Bsu36I 1980 (in frame with Factor Xa site) SEQKVEDLPADDILRVEKYL SEQKVEDLPADDILRVEKYL g t g a a c a g a a g g t g g a g g a c c t t c c t g c g g a t g a c a t c c t c c g g g t g g a g a a g t a c c t t a c a c t t g t c t t c c a c c t c c t g g a a g g a c g c c t a c t g t a g g a g g c c c a c c t c t t c a t g g a a t

ScaI 2040 (in frame with Factor Xa site) RRRKYSLFAAFYSATMLQDV RRRKYSLFAAFYSATMLQDV g g a g g c g c a a g t a c t c c c t g t t t g c g g c c t t c t a c t c a g c c a c c a t g c t g c a g g a t g t g g c c t c c g c g t t c a t g a g g g a c a a a c g c c g g a a g a t g a g t c g g t g g t a c g a c g t c c t a c a c c

2100 (in frame with Factor Xa site) DDAIQFEVSIGNYGNKFDMT DDAIQFEVSIGNYGNKFDMT a t g a t g c c a t c c a g t t t g a g g t c a g c a t c g g g a a c t a c g g g a a c a a g t t c g a c a t g a c c t t a c t a c g g t a g g t c a a a c t c c a g t c g t a g c c c t t g a t g c c c t t g t t c a a g c t g t a c t g g a

2160 (in frame with Factor Xa site) CLPLASTTQYSRAVFDGCHY CLPLASTTQYSRAVFDGCHY g c c t g c c g c t g g c c t c c a c c a c t c a g t a c a g c c g t g c a g t c t t t g a c g g g t g c c a c t a c t c g g a c g g c g a c c g g a g g t g g t g a g t c a t g t c g g c a c g t c a g a a a c t g c c c a c g g t g a t g a

2220 (in frame with Factor Xa site) YYLPWGNVKPVVVLSSYWED YYLPWGNVKPVVVLSSYWED a c t a c c t a c c c t g g g g t a a c g t g a a a c c t g t g g t g g t g c t g t c a t c c t a c t g g g a g g a c a t g a t g g a t g g g a c c c c a t t g c a c t t t g g a c a c c a c c a c g a c a g t a g g a t g a c c c t c c t g t

2280 (in frame with Factor Xa site) ISHRIETQNQLLGIADRLEA ISHRIETQNQLLGIADRLEA t c a g c c a t a g a a t c g a g a c t c a g a a c c a g c t g c t t g g g a t t g c t g a c c g g c t g g a a g c t g a g t c g g t a t c t t a g c t c t g a g t c t t g g t c g a c g a a c c c t a a c g a c t g g c c g a c c t t c g a c

2340 (in frame with Factor Xa site) GLEQVHLALKAQCSTEDVDS GLEQVHLALKAQCSTEDVDS g c c t g g a g c a g g t c c a c c t g g c c c t g a a g g c g c a g t g c t c c a c g g a g g a c g t g g a c t c g c c g g a c c t c g t c c a g g t g g a c c g g g a c t t c c g c g t c a c g a g g t g c c t c c t g c a c c t g a g c g

2400 (in frame with Factor Xa site) LVAQLTDELIAGCSQPLGDI LVAQLTDELIAGCSQPLGDI t g g t g g c t c a g c t g a c g g a t g a g c t c a t c g c a g g c t g c a g c c a g c c t c t g g g t g a c a t c c a c c a c c g a g t c g a c t g c c t a c t c g a g t a g c g t c c g a c g t c g g t c g g a g a c c c a c t g t a g g

2460 (in frame with Factor Xa site) HETPSATHLDQYLYQLRTHH HETPSATHLDQYLYQLRTHH a t g a g a c a c c c t c t g c c a c c c a c c t g g a c c a g t a c c t g t a c c a g c t g c g c a c c c a t c a c c t a c t c t g t g g g a g a c g g t g g g t g g a c c t g g t c a t g g a c a t g g t c g a c g c g t g g g t a g t g g

2520 (in frame with Factor Xa site) LSQITEAALALKLGHSELPA LSQITEAALALKLGHSELPA t g a g c c a a a t c a c t g a g g c t g c c c t g g c c c t g a a g c t c g g c c a c a g t g a g c t c c c t g c a g a c t c g g t t t a g t g a c t c c g a c g g g a c c g g g a c t t c g a g c c g g t g t c a c t c g a g g g a c g t c

2580 (in frame with Factor Xa site) ALEQAEDWLLRLRALAEEPQ ALEQAEDWLLRLRALAEEPQ c t c t g g a g c a g g c g g a g g a c t g g c t c c t g c g t c t g c g t g c c c t g g c a g a g g a g c c c c a g a g a g a c c t c g t c c g c c t c c t g a c c g a g g a c g c a g a c g c a c g g g a c c g t c t c c t c g g g g t c t

Tth111IPflFI 2640 (in frame with Factor Xa site) NSLPDIVIWMLQGDKRVAYQ NSLPDIVIWMLQGDKRVAYQ a c a g c c t g c c g g a c a t c g t c a t c t g g a t g c t g c a g g g a g a c a a g c g t g t g g c a t a c c a g c t g t c g g a c g g c c t g t a g c a g t a g a c c t a c g a c g t c c c t c t g t t c g c a c a c c g t a t g g t c g

2700 (in frame with Factor Xa site) RVPAHQVLFSRRGANYCGKN RVPAHQVLFSRRGANYCGKN g g g t g c c c g c c c a c c a a g t c c t c t t c t c c c g g c g g g g t g c c a a c t a c t g t g g c a a g a a t t c c c a c g g g c g g g t g g t t c a g g a g a a g a g g g c c g c c c c a c g g t t g a t g a c a c c g t t c t t a a

2760 (in frame with Factor Xa site) CGKLQTIFLKYPMEKVPGAR CGKLQTIFLKYPMEKVPGAR g t g g g a a g c t a c a g a c a a t c t t t c t g a a a t a t c c g a t g g a g a a g g t g c c t g g c g c c c g g a c a c c c t t c g a t g t c t g t t a g a a a g a c t t t a t a g g c t a c c t c t t c c a c g g a c c g c g g g c c t

2820 (in frame with Factor Xa site) MPVQIRVKLWFGLSVDEKEF MPVQIRVKLWFGLSVDEKEF t g c c a g t g c a g a t a c g g g t c a a g c t g t g g t t t g g g c t c t c t g t g g a t g a g a a g g a g t t c a a c g g t c a c g t c t a t g c c c a g t t c g a c a c c a a a c c c g a g a g a c a c c t a c t c t t c c t c a a g t

2880 (in frame with Factor Xa site) NQFAEGKLSVFAETYENETK NQFAEGKLSVFAETYENETK a c c a g t t t g c t g a g g g g a a g c t g t c t g t c t t t g c t g a a a c c t a t g a g a a c g a g a c t a a g t t g g t c a a a c g a c t c c c c t t c g a c a g a c a g a a a c g a c t t t g g a t a c t c t t g c t c t g a t t c a

2940 (in frame with Factor Xa site) LALVGNWGTTGLTYPKFSDV LALVGNWGTTGLTYPKFSDV t g g c c c t t g t t g g g a a c t g g g g c a c a a c g g g c c t c a c c t a c c c c a a g t t t t c t g a c g t c a a c c g g g a a c a a c c c t t g a c c c c g t g t t g c c c g g a g t g g a t g g g g t t c a a a a g a c t g c a g t

3000 (in frame with Factor Xa site) TGKIKLPKDSFRPSAGWTWA TGKIKLPKDSFRPSAGWTWA *GLVAEAGRGAPGPS c g g g c a a g a t c a a g c t a c c c a a g g a c a g c t t c c g c c c c t c g g c c g g c t g g a c c t g g g c t g g c c c g t t c t a g t t c g a t g g g t t c c t g t c g a a g g c g g g g a g c c g g c c g a c c t g g a c c c g a c

3060 (in frame with Factor Xa site) GDWFVCPEKTLLHDMDAGHL GDWFVCPEKTLLHDMDAGHL SIPEHTRLLSQEMVHVGTVQ g a g a t t g g t t c g t g t g t c c g g a g a a g a c t c t g c t c c a t g a c a t g g a c g c c g g t c a c c t g a c t c t a a c c a a g c a c a c a g g c c t c t t c t g a g a c g a g g t a c t g t a c c t g c g g c c a g t g g a c t

3120 (in frame with Factor Xa site) SFVEEVFENQTRLPGGQWIY SFVEEVFENQTRLPGGQWIY AEHFLHKLVLGPKGSALPDV g c t t c g t g g a a g a g g t g t t t g a g a a c c a g a c c c g g c t t c c c g g a g g c c a g t g g a t c t a c a c g a a g c a c c t t c t c c a c a a a c t c t t g g t c t g g g c c g a a g g g c c t c c g g t c a c c t a g a t g t

AleI 3180 (in frame with Factor Xa site) MSDNYTDVNGEKVLPKDDIE MSDNYTDVNGEKVLPKDDIE HTVVVGIHVPLLHKGLIVNL t g a g t g a c a a c t a c a c c g a t g t g a a c g g g g a g a a g g t g c t t c c c a a g g a t g a c a t t g a g t a c t c a c t g t t g a t g t g g c t a c a c t t g c c c c t c t t c c a c g a a g g g t t c c t a c t g t a a c t c a

3240 (in frame with Factor Xa site) CPLGWKWEDEEWSTDLNRAV CPLGWKWEDEEWSTDLNRAV AWQAPLPFILFPGCVEVPSD g c c c a c t g g g c t g g a a g t g g g a a g a t g a g g a a t g g t c c a c a g a c c t c a a c c g g g c t g t c g c g g g t g a c c c g a c c t t c a c c c t t c t a c t c c t t a c c a g g t g t c t g g a g t t g g c c c g a c a g c

3300 (in frame with Factor Xa site) DEQGWEYSITIPPERKPKHW DEQGWEYSITIPPERKPKHW ILLAPLIADGDGRLPLRLVP a t g a g c a a g g c t g g g a g t a t a g c a t c a c c a t c c c c c c g g a g c g g a a g c c g a a g c a c t g g g t a c t c g t t c c g a c c c t c a t a t c g t a g t g g t a g g g g g g c c t c g c c t t c g g c t t c g t g a c c c

3360 (in frame with Factor Xa site) VPAEKMYYTHRRRRWVRLRR VPAEKMYYTHRRRRWVRLRR DRSLLHVVCVSPPAPHAQAP t c c c t g c t g a g a a g a t g t a c t a c a c a c a c c g a c g g c g g c g c t g g g t g c g c c t g c g c a g g a a g g g a c g a c t c t t c t a c a t g a t g t g t g t g g c t g c c g c c g c g a c c c a c g c g g a c g c g t c c t

3420 (in frame with Factor Xa site) RDLSQMEALKRHRQAEAEGE RDLSQMEALKRHRQAEAEGE PIEALHFCQFPVPLRLRLAL g g g a t c t c a g c c a a a t g g a a g c a c t g a a a a g g c a c a g g c a g g c g g a g g c g g a g g g c g a g g c c c t a g a g t c g g t t t a c c t t c g t g a c t t t t c c g t g t c c g t c c g c c t c c g c c t c c c g c t c c

PaeR7IPspXIXhoI 3480 (in frame with Factor Xa site) GWEYASLFGWKFHLEYRKTD GWEYASLFGWKFHLEYRKTD APLVGRKKAPLEVELVALCI g c t g g g a g t a c g c c t c t c t t t t t g g c t g g a a g t t c c a c c t c g a g t a c c g c a a g a c a g a t g c g a c c c t c a t g c g g a g a g a a a a a c c g a c c t t c a a g g t g g a g c t c a t g g c g t t c t g t c t a c

3540 (in frame with Factor Xa site) AFRRRRWRRRMEPLEKTGPA AFRRRRWRRRMEPLEKTGPA GEAAAAPATAHLWQLLRPRC c c t t c c g c c g c c g c c g c t g g c g c c g t c g c a t g g a g c c a c t g g a g a a g a c g g g g c c t g c a g g g a a g g c g g c g g c g g c g a c c g c g g c a g c g t a c c t c g g t g a c c t c t t c t g c c c c g g a c g t c

3600 (in frame with Factor Xa site) AVFALEGALGGVMDDKSEDS AVFALEGALGGVMDDKSEDS SHKGKLPGQAAHHIVLTFIG c t g t g t t t g c c c t t g a g g g g g c c c t g g g c g g c g t g a t g g a t g a c a a g a g t g a a g a t t c c a g a c a c a a a c g g g a a c t c c c c c g g g a c c c g c c g c a c t a c c t a c t g t t c t c a c t t c t a a g g t

3660 (in frame with Factor Xa site) MSVSTLSFGVNRPTISCIFD MSVSTLSFGVNRPTISCIFD HGDGGQAETHVSGRNGAYEV t g t c c g t c t c c a c c t t g a g c t t c g g t g t g a a c a g a c c c a c g a t t t c c t g c a t a t t c g a c t a c a g g c a g a g g t g g a a c t c g a a g c c a c a c t t g t c t g g g t g c t a a a g g a c g t a t a a g c t g a

3720 (in frame with Factor Xa site) YGNRYHLRCYMYQARDLAAM YGNRYHLRCYMYQARDLAAM IPVAVM a t g g g a a c c g c t a c c a t c t a c g c t g c t a c a t g t a c c a g g c c c g g g a c c t g g c t g c g a t g g t a c c c t t g g c g a t g g t a g a t g c g a c g a t g t a c a t g g t c c g g g c c c t g g a c c g a c g c t a c c

3780 (in frame with Factor Xa site) DKDSFSDPYAIVSFLHQSQK DKDSFSDPYAIVSFLHQSQK a c a a g g a c t c t t t t t c t g a t c c c t a t g c c a t c g t c t c c t t c c t g c a c c a g a g c c a g a a g a t g t t c c t g a g a a a a a g a c t a g g g a t a c g g t a g c a g a g g a a g g a c g t g g t c t c g g t c t t c t

3840 (in frame with Factor Xa site) TVVVKNTLNPTWDQTLIFYE TVVVKNTLNPTWDQTLIFYE c g g t g g t g g t g a a g a a c a c c c t t a a c c c c a c c t g g g a c c a g a c g c t c a t c t t c t a c g a g a g c c a c c a c c a c t t c t t g t g g g a a t t g g g g t g g a c c c t g g t c t g c g a g t a g a a g a t g c t c t

3900 (in frame with Factor Xa site) IEIFGEPATVAEQPPSIVVE IEIFGEPATVAEQPPSIVVE t c g a g a t c t t t g g c g a g c c g g c c a c a g t t g c t g a g c a a c c g c c c a g c a t t g t g g t g g a g c a g c t c t a g a a a c c g c t c g g c c g g t g t c a a c g a c t c g t t g g c g g g t c g t a a c a c c a c c t c g

3960 (in frame with Factor Xa site) LYDHDTYGADEFMGRCICQP LYDHDTYGADEFMGRCICQP t g t a c g a c c a t g a c a c t t a t g g t g c a g a c g a g t t t a t g g g t c g c t g c a t c t g t c a a c c g a a c a t g c t g g t a c t g t g a a t a c c a c g t c t g c t c a a a t a c c c a g c g a c g t a g a c a g t t g g c t

4020 (in frame with Factor Xa site) SLERMPRLAWFPLTRGSQPS SLERMPRLAWFPLTRGSQPS g t c t g g a a c g g a t g c c a c g g c t g g c c t g g t t c c c a c t g a c g a g g g g c a g c c a g c c g t c g g c a g a c c t t g c c t a c g g t g c c g a c c g g a c c a a g g g t g a c t g c t c c c c g t c g g t c g g c a g c c

4080 (in frame with Factor Xa site) GELLASFELIQREKPAIHHI GELLASFELIQREKPAIHHI g g g a g c t g c t g g c c t c t t t t g a g c t c a t c c a g a g a g a g a a g c c g g c c a t c c a c c a t a t t c c c c t c g a c g a c c g g a g a a a a c t c g a g t a g g t c t c t c t c t t c g g c c g g t a g g t g g t a t a a g

4140 (in frame with Factor Xa site) PGFESEDTDLPYPPPQREAN PGFESEDTDLPYPPPQREAN c t g g t t t t g a g t c t g a g g a c a c a g a c c t g c c c t a c c c a c c a c c c c a g a g g g a g g c c a a c a g a c c a a a a c t c a g a c t c c t g t g t c t g g a c g g g a t g g g t g g t g g g g t c t c c c t c c g g t t g t

AfeI 4200 (in frame with Factor Xa site) IYMVPQNIKPALQRTAIEIL IYMVPQNIKPALQRTAIEIL t c t a c a t g g t t c c t c a g a a c a t c a a g c c a g c g c t c c a g c g t a c c g c c a t c g a g a t c c t g g a g a t g t a c c a a g g a g t c t t g t a g t t c g g t c g c g a g g t c g c a t g g c g g t a g c t c t a g g a c c

4260 (in frame with Factor Xa site) AWGLRNMKSYQLANISSPSL AWGLRNMKSYQLANISSPSL c a t g g g g c c t g c g g a a c a t g a a g a g t t a c c a g c t g g c c a a c a t c t c c t c c c c c a g c c t c g g t a c c c c g g a c g c c t t g t a c t t c t c a a t g g t c g a c c g g t t g t a g a g g a g g g g g t c g g a g c

4320 (in frame with Factor Xa site) VVECGGQTVQSCVIRNLRKN VVECGGQTVQSCVIRNLRKN t g g t a g a g t g t g g g g g c c a g a c g g t g c a g t c c t g t g t c a t c a g g a a c c t c c g g a a g a a c c a c c a t c t c a c a c c c c c g g t c t g c c a c g t c a g g a c a c a g t a g t c c t t g g a g g c c t t c t t g g

4380 (in frame with Factor Xa site) PNFDICTLFMEVMLPREELY PNFDICTLFMEVMLPREELY c c a a c t t t g a c a t c t g c a c c c t c t t c a t g g a a g t g a t g c t g c c c a g g g a g g a g c t c t a c t g g t t g a a a c t g t a g a c g t g g g a g a a g t a c c t t c a c t a c g a c g g g t c c c t c c t c g a g a t g a

4440 (in frame with Factor Xa site) CPPITVKVIDNRQFGRRPVV CPPITVKVIDNRQFGRRPVV g c c c c c c c a t c a c c g t c a a g g t c a t c g a t a a c c g c c a g t t t g g c c g c c g g c c t g t g g t g g c g g g g g g g t a g t g g c a g t t c c a g t a g c t a t t g g c g g t c a a a c c g g c g g c c g g a c a c c a c c

4500 (in frame with Factor Xa site) GQCTIRSLESFLCDPYSAES GQCTIRSLESFLCDPYSAES g c c a g t g t a c c a t c c g c t c c c t g g a g a g c t t c c t g t g t g a c c c c t a c t c g g c g g a g a g t c c g g t c a c a t g g t a g g c g a g g g a c c t c t c g a a g g a c a c a c t g g g g a t g a g c c g c c t c t c a g

DraIII 4560 (in frame with Factor Xa site) PSPQGGPDDVSLLSPGEDVL PSPQGGPDDVSLLSPGEDVL c a t c c c c a c a g g g t g g c c c a g a c g a t g t g a g c c t a c t c a g t c c t g g g g a a g a c g t g c t c a g t a g g g g t g t c c c a c c g g g t c t g c t a c a c t c g g a t g a g t c a g g a c c c c t t c t g c a c g a g t

4620 (in frame with Factor Xa site) IDIDDKEPLIPIQEEEFIDW IDIDDKEPLIPIQEEEFIDW t c g a c a t t g a t g a c a a g g a g c c c c t c a t c c c c a t c c a g g a g g a a g a g t t c a t c g a t t g g t a g c t g t a a c t a c t g t t c c t c g g g g a g t a g g g g t a g g t c c t c c t t c t c a a g t a g c t a a c c a

4680 (in frame with Factor Xa site) WSKFFASIGEREKCGSYLEK WSKFFASIGEREKCGSYLEK g g a g c a a a t t c t t t g c c t c c a t a g g g g a g a g g g a a a a g t g c g g c t c c t a c c t g g a g a a g g c c t c g t t t a a g a a a c g g a g g t a t c c c c t c t c c c t t t t c a c g c c g a g g a t g g a c c t c t t c c

StuI 4740 (in frame with Factor Xa site) DFDTLKVYDTQLENVEAFEG DFDTLKVYDTQLENVEAFEG a t t t t g a c a c c c t g a a g g t c t a t g a c a c a c a g c t g g a g a a t g t g g a g g c c t t t g a g g g c c t a a a a c t g t g g g a c t t c c a g a t a c t g t g t g t c g a c c t c t t a c a c c t c c g g a a a c t c c c g g

4800 (in frame with Factor Xa site) LSDFCNTFKLYRGKTQEETE LSDFCNTFKLYRGKTQEETE t g t c t g a c t t t t g t a a c a c c t t c a a g c t g t a c c g g g g c a a g a c g c a g g a g g a g a c a g a a g a c a g a c t g a a a a c a t t g t g g a a g t t c g a c a t g g c c c c g t t c t g c g t c c t c c t c t g t c t t c

4860 (in frame with Factor Xa site) DPSVIGEFKGLFKIYPLPED DPSVIGEFKGLFKIYPLPED a t c c a t c t g t g a t t g g t g a a t t t a a g g g c c t c t t c a a a a t t t a t c c c c t c c c a g a a g a c c t a g g t a g a c a c t a a c c a c t t a a a t t c c c g g a g a a g t t t t a a a t a g g g g a g g g t c t t c t g g

4920 (in frame with Factor Xa site) PAIPMPPRQFHQLAAQGPQE PAIPMPPRQFHQLAAQGPQE c a g c c a t c c c c a t g c c c c c a a g a c a g t t c c a c c a g c t g g c c g c c c a g g g a c c c c a g g a g t g t c g g t a g g g g t a c g g g g g t t c t g t c a a g g t g g t c g a c c g g c g g g t c c c t g g g g t c c t c a

4980 (in frame with Factor Xa site) CLVRIYIVRAFGLQPKDPNG CLVRIYIVRAFGLQPKDPNG g c t t g g t c c g t a t c t a c a t t g t c c g a g c a t t t g g c c t g c a g c c c a a g g a c c c c a a t g g a a c g a a c c a g g c a t a g a t g t a a c a g g c t c g t a a a c c g g a c g t c g g g t t c c t g g g g t t a c c t t

5040 (in frame with Factor Xa site) KCDPYIKISIGKKSVSDQDN KCDPYIKISIGKKSVSDQDN a g t g t g a t c c t t a c a t c a a g a t c t c c a t a g g g a a g a a a t c a g t g a g t g a c c a g g a t a a c t t c a c a c t a g g a a t g t a g t t c t a g a g g t a t c c c t t c t t t a g t c a c t c a c t g g t c c t a t t g a

5100 (in frame with Factor Xa site) YIPCTLEPVFGKMFELTCTL YIPCTLEPVFGKMFELTCTL a c a t c c c c t g c a c g c t g g a g c c c g t a t t t g g a a a g a t g t t c g a g c t g a c c t g c a c t c t g c t g t a g g g g a c g t g c g a c c t c g g g c a t a a a c c t t t c t a c a a g c t c g a c t g g a c g t g a g a c g

5160 (in frame with Factor Xa site) PLEKDLKITLYDYDLLSKDE PLEKDLKITLYDYDLLSKDE c t c t g g a g a a g g a c c t a a a g a t c a c t c t c t a t g a c t a t g a c c t c c t c t c c a a g g a c g a a a g a g a c c t c t t c c t g g a t t t c t a g t g a g a g a t a c t g a t a c t g g a g g a g a g g t t c c t g c t t t

5220 (in frame with Factor Xa site) KIGETVVDLENRLLSKFGAR KIGETVVDLENRLLSKFGAR a g a t c g g t g a g a c g g t c g t c g a c c t g g a g a a c a g g c t g c t g t c c a a g t t t g g g g c t c g c t t c t a g c c a c t c t g c c a g c a g c t g g a c c t c t t g t c c g a c g a c a g g t t c a a a c c c c g a g c g a

5280 (in frame with Factor Xa site) CGLPQTYCVSGPNQWRDQLR CGLPQTYCVSGPNQWRDQLR g t g g a c t c c c a c a g a c c t a c t g t g t c t c t g g a c c g a a c c a g t g g c g g g a c c a g c t c c g c c c a c c t g a g g g t g t c t g g a t g a c a c a g a g a c c t g g c t t g g t c a c c g c c c t g g t c g a g g c g g

5340 (in frame with Factor Xa site) PSQLLHLFCQQHRVKAPVYR PSQLLHLFCQQHRVKAPVYR c c t c c c a g c t c c t c c a c c t c t t c t g c c a g c a g c a t a g a g t c a a g g c a c c t g t g t a c c g g a g g a g g g t c g a g g a g g t g g a g a a g a c g g t c g t c g t a t c t c a g t t c c g t g g a c a c a t g g c c t

5400 (in frame with Factor Xa site) TDRVMFQDKEYSIEEIEAGR TDRVMFQDKEYSIEEIEAGR c a g a c c g t g t a a t g t t t c a g g a t a a a g a a t a t t c c a t t g a a g a g a t a g a g g c t g g c a g g a g t c t g g c a c a t t a c a a a g t c c t a t t t c t t a t a a g g t a a c t t c t c t a t c t c c g a c c g t c c t

5460 (in frame with Factor Xa site) IPNPHLGPVEERLALHVLQQ IPNPHLGPVEERLALHVLQQ t c c c a a a c c c a c a c c t g g g c c c a g t g g a g g a g c g t c t g g c t c t g c a t g t g c t t c a g c a g c a g g g t t t g g g t g t g g a c c c g g g t c a c c t c c t c g c a g a c c g a g a c g t a c a c g a a g t c g t c g

BsaAIPmlI 5520 (in frame with Factor Xa site) QGLVPEHVESRPLYSPLQPD QGLVPEHVESRPLYSPLQPD a g g g c c t g g t c c c g g a g c a c g t g g a g t c a c g g c c c c t c t a c a g c c c c c t g c a g c c a g a c a t c c c g g a c c a g g g c c t c g t g c a c c t c a g t g c c g g g g a g a t g t c g g g g g a c g t c g g t c t g t

5580 (in frame with Factor Xa site) IEQGKLQMWVDLFPKALGRP IEQGKLQMWVDLFPKALGRP *KRLGQPPR t c g a g c a g g g g a a g c t g c a g a t g t g g g t c g a c c t a t t t c c g a a g g c c c t g g g g c g g c c t g a g c t c g t c c c c t t c g a c g t c t a c a c c c a g c t g g a t a a a g g c t t c c g g g a c c c c g c c g g a c

5640 (in frame with Factor Xa site) GPPFNITPRRARRFFLRCII GPPFNITPRRARRFFLRCII SRGEVDGWPSGSPKEQTTND g a c c t c c c t t c a a c a t c a c c c c a c g g a g a g c c a g a a g g t t t t t c c t g c g t t g t a t t a t c t c t g g a g g g a a g t t g t a g t g g g g t g c c t c t c g g t c t t c c a a a a a g g a c g c a a c a t a a t a g a

5700 (in frame with Factor Xa site) WNTRDVILDDLSLTGEKMSD WNTRDVILDDLSLTGEKMSD PIGSIHDQIVQAERPLLHAV g g a a t a c c a g a g a t g t g a t c c t g g a t g a c c t g a g c c t c a c g g g g g a g a a g a t g a g c g a c a c c t t a t g g t c t c t a c a c t a g g a c c t a c t g g a c t c g g a g t g c c c c c t c t t c t a c t c g c t g t

5760 (in frame with Factor Xa site) IYVKGWMIGFEEHKQKTDVH IYVKGWMIGFEEHKQKTDVH NIHFTPHNAKFFVLLLCVHM t t t a t g t g a a a g g t t g g a t g a t t g g c t t t g a a g a a c a c a a g c a a a a g a c a g a c g t g c a t t a a a t a c a c t t t c c a a c c t a c t a a c c g a a a c t t c t t g t g t t c g t t t t c t g t c t g c a c g t a a

5820 (in frame with Factor Xa site) YRSLGGEGNFNWRFIFPFDY YRSLGGEGNFNWRFIFPFDY ITGQSTFAVEVPPENEGEVV *R a t c g t t c c c t g g g a g g t g a a g g c a a c t t c a a c t g g a g g t t c a t t t t c c c c t t c g a c t a c c t a g c a a g g g a c c c t c c a c t t c c g t t g a a g t t g a c c t c c a a g t a a a a g g g g a a g c t g a t g g

BsrDI 5880 (in frame with Factor Xa site) LPAEQVCTIAKKDAFWRLDK LPAEQVCTIAKKDAFWRLDK QWSLLDTGNGLLIGEPPQVL GASCTQVMALFSAKQLSSLV t g c c a g c t g a g c a a g t c t g t a c c a t t g c c a a g a a g g a t g c c t t c t g g a g g c t g g a c a a g a a c g g t c g a c t c g t t c a g a c a t g g t a a c g g t t c t t c c t a c g g a a g a c c t c c g a c c t g t t c t

5940 (in frame with Factor Xa site) TESKIPARVVFQIWDNDKFS TESKIPARVVFQIWDNDKFS SLAFDWCSHHELDPVIVLEG SLLIGARTTNWIQSLSLNEK c t g a g a g c a a a a t c c c a g c a c g a g t g g t g t t c c a g a t c t g g g a c a a t g a c a a g t t c t c c t g a c t c t c g t t t t a g g g t c g t g c t c a c c a c a a g g t c t a g a c c c t g t t a c t g t t c a a g a g g a

SphI 6000 (in frame with Factor Xa site) FDDFLGSLQLDLNRMPKPAK FDDFLGSLQLDLNRMPKPAK KIIKQAGQLEIEVAHGLWGL SSKRPERCSSRLRMGLGALV t t g a t g a t t t t c t g g g c t c c c t g c a g c t c g a t c t c a a c c g c a t g c c c a a g c c a g c c a a g a a a c t a c t a a a a g a c c c g a g g g a c g t c g a g c t a g a g t t g g c g t a c g g g t t c g g t c g g t t c t

6060 (in frame with Factor Xa site) TAKKCSLDQLDDAFHPEWFV TAKKCSLDQLDDAFHPEWFV CGLLAGQVLQIISEVWFPKH ALFHEKSWSSSAKWGSHNTD c a g c c a a g a a g t g c t c c t t g g a c c a g c t g g a t g a t g c t t t c c a c c c a g a a t g g t t t g t g t g t c g g t t c t t c a c g a g g a a c c t g g t c g a c c t a c t a c g a a a g g t g g g t c t t a c c a a a c a c a

6120 (in frame with Factor Xa site) SLFEQKTVKGWWPCVAEEGE SLFEQKTVKGWWPCVAEEGE GKKLLFCHLAPPGTYCFLTL RKSCFVTFPQHGQTASSPSF c c c t t t t t g a g c a g a a a a c a g t g a a g g g c t g g t g g c c c t g t g t a g c a g a a g a g g g t g a g a g g g a a a a a c t c g t c t t t t g t c a c t t c c c g a c c a c c g g g a c a c a t c g t c t t c t c c c a c t c t

6180 (in frame with Factor Xa site) KKILAGKLEMTLEIVAESEH KKILAGKLEMTLEIVAESEH LFYQRALQFHGQLNYCLTLM FISAPLSSIVKSITASLSCS a g a a a a t a c t g g c g g g c a a g c t g g a a a t g a c c t t g g a g a t t g t a g c a g a g a g t g a g c a t g t c t t t t a t g a c c g c c c g t t c g a c c t t t a c t g g a a c c t c t a a c a t c g t c t c t c a c t c g t a c

HindIII 6240 (in frame with Factor Xa site) EERPAGQGRDEPNMNPKLED EERPAGQGRDEPNMNPKLED SRGAPWPRSSGLMFGLSSSG a g g a g c g g c c t g c t g g c c a g g g c c g g g a t g a g c c c a a c a t g a a c c c t a a g c t t g a g g a c c t c c t c g c c g g a c g a c c g g t c c c g g c c c t a c t c g g g t t g t a c t t g g g a t t c g a a c t c c t g g

6300 (in frame with Factor Xa site) PRRPDTSFLWFTSPYKTMKF PRRPDTSFLWFTSPYKTMKF LRGSVEKRHNVEGYLVMFNM c a a g g c g c c c c g a c a c c t c c t t c c t g t g g t t t a c c t c c c c a t a c a a g a c c a t g a a g t t c a g t t c c g c g g g g c t g t g g a g g a a g g a c a c c a a a t g g a g g g g t a t g t t c t g g t a c t t c a a g t

6360 (in frame with Factor Xa site) ILWRRFRWAIILFIILFILL ILWRRFRWAIILFIILFILL RHRRKRHAMMRKMMRKMRSS t c c t g t g g c g g c g t t t c c g g t g g g c c a t c a t c c t c t t c a t c a t c c t c t t c a t c c t g c t g c a g g a c a c c g c c g c a a a g g c c a c c c g g t a g t a g g a g a a g t a g t a g g a g a a g t a g g a c g a c g

6420 (in frame with Factor Xa site) LFLAIFIYAFPNYAAMKLVK LFLAIFIYAFPNYAAMKLVK NRAMKM t g t t c c t g g c c a t c t t c a t c t a c g c c t t c c c g a a c t a t g c t g c c a t g a a g c t g g t g a a g c a c a a g g a c c g g t a g a a g t a g a t g c g g a a g g g c t t g a t a c g a c g g t a c t t c g a c c a c t t c g

NdeINheIBmtINotIBclI* 6480 PFS* M13 rev Kozak sequence C A G T A T C G A C A A A M13 Reverse PFS* c c t t c a g c t g a t g a t c a c c a t g g c a t a t g g c t a g c g c g g c c g c c a t g g t c a t a g c t g t t t g g a a g t c g a c t a c t a g t g g t a c c g t a t a c c g a t c g c g c c g g c g g t a c c a g t a t c g a c a a a

6540 -10 M13 rev lac operator lac promoter G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse c c t g t g t g a a a t t g t t a t c c g c t c a c a a t t c c a c a c a a c a t a c g a g c c g g a a g c a t a a a g g g a c a c a c t t t a a c a a t a g g c g a g t g t t a a g g t g t g t t g t a t g c t c g g c c t t c g t a t t t c

6600 -35 lac promoter CAP binding site t g t a a a g c c t g g g g t g c c t a a t g a g t g a g c t a a c t c a c a t t a a t t g c g t t g c g c t c a c t g a c a t t t c g g a c c c c a c g g a t t a c t c a c t c g a t t g a g t g t a a t t a a c g c a a c g c g a g t g a c

6660 c c c g c t t t c c a g t c g g g a a a c c t g t c g t g c c a g c t g c a t t a a t g a a t c g g c c a a c g c g c g g g g c g a a a g g t c a g c c c t t t g g a c a g c a c g g t c g a c g t a a t t a c t t a g c c g g t t g c g c g c

BspQISapI 6720 G A G C G A G T G A C T G A G C G A L4440 g g g a g a g g c g g t t t g c g t a t t g g g c g c t c t t c c g c t t c c t c g c t c a c t g a c t c g c t g c g c c c c t c t c c g c c a a a c g c a t a a c c c g c g a g a a g g c g a a g g a g c g a g t g a c t g a g c g a c g c g

6780 t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a a g g c g g t a a t a c g g t t a t c c a g c c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t t c c g c c a t t a t g c c a a t a g g

6840 a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c

6900 ori a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a

6960 ori T T T C T A T G G T C pBR322ori-F c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c

7020 ori C G C A A A G G G pBR322ori-F g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t

7080 ori t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c

7140 ori t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a

7200 ori c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g

7260 ori g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g

7320 ori g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a c a c t a g a a g a a c a g t a t t t c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t g t g a t c t t c t t g t c a t a a a

7380 ori g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g

7440 ori g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g

7500 ori a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c

7560 a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c

7620 a t c c t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t c t a a a g t a t a t a t g a g t a a a c t t g g t a g g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t t t c a t a t a t a c t c a t t t g a a c c

7680 270 265 260 *FFEDLMLHFQLKNMDP KanR 270 265 260 *FFEDLMLHFQLKNMDP KanR *FFEDLMLHFQLKNMDP t c t g a c a g t t a g a a a a a c t c a t c g a g c a t c a a a t g a a a c t g c a a t t t a t t c a t a t c a g g a a g a c t g t c a a t c t t t t t g a g t a g c t c g t a g t t t a c t t t g a c g t t a a a t a a g t a t a g t c c t

7740 255 250 245 240 NDIGYKQFLRKQLSPSFEGL KanR 255 250 245 240 NDIGYKQFLRKQLSPSFEGL KanR NDIGYKQFLRKQLSPSFEGL t t a t c a a t a c c a t a t t t t t g a a a a a g c c g t t t c t g t a a t g a a g g a g a a a a c t c a c c g a g g a a t a g t t a t g g t a t a a a a a c t t t t t c g g c a a a g a c a t t a c t t c c t c t t t t g a g t g g c t c c

7800 235 230 225 220 CNWLIALDQYRDAIGVRGVD KanR 235 230 225 220 CNWLIALDQYRDAIGVRGVD KanR CNWLIALDQYRDAIGVRGVD c a g t t c c a t a g g a t g g c a a g a t c c t g g t a t c g g t c t g c g a t t c c g a c t c g t c c a a c a t c a g t c a a g g t a t c c t a c c g t t c t a g g a c c a t a g c c a g a c g c t a a g g c t g a g c a g g t t g t a g t

7860 215 210 205 200 ICGILKGEDFILNDLSFDGH KanR 215 210 205 200 ICGILKGEDFILNDLSFDGH KanR ICGILKGEDFILNDLSFDGH a t a c a a c c t a t t a a t t t c c c c t c g t c a a a a a t a a g g t t a t c a a g t g a g a a a t c a c c a t g a t a t g t t g g a t a a t t a a a g g g g a g c a g t t t t t a t t c c a a t a g t t c a c t c t t t a g t g g t a c t

7920 195 190 185 180 TVVSDPSFPLLKHMEKWVQE KanR 195 190 185 180 TVVSDPSFPLLKHMEKWVQE KanR TVVSDPSFPLLKHMEKWVQE g t g a c g a c t g a a t c c g g t g a g a a t g g c a a a a g t t t a t g c a t t t c t t t c c a g a c t t g t t c a c a c t g c t g a c t t a g g c c a c t c t t a c c g t t t t c a a a t a c g t a a a g a a a g g t c t g a a c a a g t

7980 175 170 165 160 VPWGNREDDFDSADVLGNNM KanR 175 170 165 160 VPWGNREDDFDSADVLGNNM KanR VPWGNREDDFDSADVLGNNM a c a g g c c a g c c a t t a c g c t c g t c a t c a a a a t c a c t c g c a t c a a c c a a a c c g t t a t t c a t t t g t c c g g t c g g t a a t g c g a g c a g t a g t t t t a g t g a g c g t a g t t g g t t t g g c a a t a a g t a a

AsiSIPvuI 8040 155 150 145 140 RSQAQALRFVRDSNFPCNCV KanR 155 150 145 140 RSQAQALRFVRDSNFPCNCV KanR RSQAQALRFVRDSNFPCNCV c g t g a t t g c g c c t g a g c g a g a c g a a a t a c g c g a t c g c t g t t a a a a g g a c a a t t a c a a a c a g c a c t a a c g c g g a c t c g c t c t g c t t t a t g c g c t a g c g a c a a t t t t c c t g t t a a t g t t t g t

8100 135 130 125 120 PISHLRRLFVALADVINEGS KanR 135 130 125 120 PISHLRRLFVALADVINEGS KanR PISHLRRLFVALADVINEGS g g a a t c g a a t g c a a c c g g c g c a g g a a c a c t g c c a g c g c a t c a a c a a t a t t t t c a c c t g a a c c t t a g c t t a c g t t g g c c g c g t c c t t g t g a c g g t c g c g t a g t t g t t a t a a a a g t g g a c t t

8160 115 110 105 100 DPYEELVQFATKGPIATTLL KanR 115 110 105 100 DPYEELVQFATKGPIATTLL KanR DPYEELVQFATKGPIATTLL t c a g g a t a t t c t t c t a a t a c c t g g a a t g c t g t t t t c c c a g g g a t c g c a g t g g t g a g t a a c a g t c c t a t a a g a a g a t t a t g g a c c t t a c g a c a a a a g g g t c c c t a g c g t c a c c a c t c a t t g

8220 95 90 85 80 WADDPTRIFHKITPLPMFET KanR 95 90 85 80 WADDPTRIFHKITPLPMFET KanR WADDPTRIFHKITPLPMFET c a t g c a t c a t c a g g a g t a c g g a t a a a a t g c t t g a t g g t c g g a a g a g g c a t a a a t t c c g t c g t a c g t a g t a g t c c t c a t g c c t a t t t t a c g a a c t a c c a g c c t t c t c c g t a t t t a a g g c a g

8280 75 70 65 60 LWNLRVMEDTVDNAVSGKGH KanR 75 70 65 60 LWNLRVMEDTVDNAVSGKGH KanR LWNLRVMEDTVDNAVSGKGH a g c c a g t t t a g t c t g a c c a t c t c a t c t g t a a c a t c a t t g g c a a c g c t a c c t t t g c c a t g t t c g g t c a a a t c a g a c t g g t a g a g t a g a c a t t g t a g t a a c c g t t g c g a t g g a a a c g g t a c a

8340 55 50 45 40 KLFLEPADPKGYLRYITAGS KanR 55 50 45 40 KLFLEPADPKGYLRYITAGS KanR KLFLEPADPKGYLRYITAGS t t c a g a a a c a a c t c t g g c g c a t c g g g c t t c c c a t a c a a t c g a t a g a t t g t c g c a c c t g a t a a g t c t t t g t t g a g a c c g c g t a g c c c g a a g g g t a t g t t a g c t a t c t a a c a g c g t g g a c t a

NruI 8400 35 30 25 20 QGVNDRAWKYGYLDADMNSN KanR 35 30 25 20 QGVNDRAWKYGYLDADMNSN KanR A T C G C G A G C C C A T T T A T A C C Kan-R QGVNDRAWKYGYLDADMNSN t g c c c g a c a t t a t c g c g a g c c c a t t t a t a c c c a t a t a a a t c a g c a t c c a t g t t g g a a t t t a c g g g c t g t a a t a g c g c t c g g g t a a a t a t g g g t a t a t t t a g t c g t a g g t a c a a c c t t a a a

8460 15 10 5 1 LRPRSCSTERQIHSM KanR AmpR promoter 15 10 5 1 LRPRSCSTERQIHSM KanR LRPRSCSTERQIHSM a a t c g c g g c c t a g a g c a a g a c g t t t c c c g t t g a a t a t g g c t c a t a c t c t t c c t t t t t c a a t t a g c g c c g g a t c t c g t t c t g c a a a g g g c a a c t t a t a c c g a g t a t g a g a a g g a a a a a g t t

8520 AmpR promoter t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a t g t a t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a a c t t a c a t a a

8580 AmpR promoter t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c c t g a c g t c a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g t g g a c t g c a g

8640 A A T A G G C G T A T C A C G A G G C pBRforEco t a a g a a a c c a t t a t t a t c a t g a c a t t a a c c t a t a a a a a t a g g c g t a t c a c g a g g c c c t t t a t t c t t t g g t a a t a a t a g t a c t g t a a t t g g a t a t t t t t a t c c g c a t a g t g c t c c g g g a a a

3ʹ 5ʹ 8644 c g t c g c a g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.