Addgene pX330A_D10A-1x7 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pX330A_D10A-1x7 Sequencing Result

Map View

600 400 200 End (624) PstI (595) XbaI (576) PluTI HaeII (570) SfoI (568) NarI (567) KasI (566) FspI (530) PvuI - BsiEI (511) EarI (489) PvuII - MspA1I (480) BmrI (430) M13/pUC Forward (403 .. 425) M13 Forward (394 .. 411) EaeI (396) T7 (365 .. 384) ApaI - BanII - BaeGI - Bsp1286I - Bme1580I (350) BpmI - Eco57MI (349) PspOMI (346) AbsI - AvaI - SmlI - XhoI - BsoBI - PspXI - PaeR7I (337) PpuMI (333) T3 (298 .. 318) M13 Reverse (263 .. 279) BsrBI (245) M13/pUC Reverse (244 .. 266) AseI (164) BtsI - BtsαI (153) AgeI - SgrAI - BsaWI (131) Start (0) CAP binding site lac promoter lac operator lacZ α M13 rev T3 promoter T7 promoter M13 fwd pX330A_D10A-1x7 624 bp

Sequence View

Start (0) 5ʹ 3ʹ 60 a a t a c t c c a c c c a t t g a c g t c a a t g g a a a g t c c c t a t t g g c g t t a c t a t t g a c g t c a a t g t t a t g a g g t g g g t a a c t g c a g t t a c c t t t c a g g g a t a a c c g c a a t g a t a a c t g c a g t t a c

120 g g c g g g g g t c g t t g g g c g g t c a g c c a g g c g g g c c a t t t a c c g t a a g t t a t g t a a c g g g t a c c g c c c c c a g c a a c c c g c c a g t c g g t c c g c c c g g t a a a t g g c a t t c a a t a c a t t g c c c a t

AseIBtsαIBtsIBsaWISgrAIAgeI 180 CAP binding site c c t a g a g a c c a c c g g t g g a a a g c g g g c a g t g a g c g c a a c g c a a t t a a t g t g a g t t a g c t c g g a t c t c t g g t g g c c a c c t t t c g c c c g t c a c t c g c g t t g c g t t a a t t a c a c t c a a t c g a g

240 -35 -10 CAP binding site lac promoter lac operator a c t c a t t a g g c a c c c c a g g c t t t a c a c t t t a t g c t t c c g g c t c g t a t g t t g t g t g g a a t t t g a g t a a t c c g t g g g g t c c g a a a t g t g a a a t a c g a a g g c c g a g c a t a c a a c a c a c c t t a a

BsrBI 300 1 5 MTMITPSAQ lac operator lacZ α M13 rev T3 promoter A G C G G A T A A C A A T T T C A C A C A G G M13/pUC Reverse C A G G A A A C A G C T A T G A C M13 Reverse G C A T3 g t g a g c g g a t a a c a a t t t c a c a c a g g a a a c a g c t a t g a c c a t g a t t a c g c c a a g c g c g c a c a c t c g c c t a t t g t t a a a g t g t g t c c t t t g t c g a t a c t g g t a c t a a t g c g g t t c g c g c g t

PpuMIPaeR7IBsoBIPspXIXhoISmlIAvaIAbsIPspOMIEco57MIBpmIBme1580IBsp1286IBaeGIBanIIApaI 360 10 15 20 25 LTLTKGNKSWRTSRGGPVPN lacZ α T3 promoter A T T A A C C C T C A C T A A A G G T3 a t t a a c c c t c a c t a a a g g g a a c a a a a g c t g g a g g a c c t c g a g g g g g g g c c c g g t a c c c a a t a a t t g g g a g t g a t t t c c c t t g t t t t c g a c c t c c t g g a g c t c c c c c c c g g g c c a t g g g t t

EaeI 420 30 35 40 45 SPYSESYYARSLAVVLQRRD lacZ α T7 promoter M13 fwd G G G A T A T C A C T C A G C A T A A T T7 T G A C C G G C A G C A A A A T G T M13 Forward G C A A A A T G T T G C A G C A C T M13/pUC Forward t t c g c c c t a t a g t g a g t c g t a t t a c g c g c g c t c a c t g g c c g t c g t t t t a c a a c g t c g t g a a a g c g g g a t a t c a c t c a g c a t a a t g c g c g c g a g t g a c c g g c a g c a a a a t g t t g c a g c a c t

MspA1IPvuIIBmrI 480 50 55 60 65 WENPGVTQLNRLAAHPPFAS lacZ α G A C C C M13/pUC Forward c t g g g a a a a c c c t g g c g t t a c c c a a c t t a a t c g c c t t g c a g c a c a t c c c c c t t t c g c c a g g a c c c t t t t g g g a c c g c a a t g g g t t g a a t t a g c g g a a c g t c g t g t a g g g g g a a a g c g g t c

FspIBsiEIPvuIEarI 540 70 75 80 85 WRNSEEARTDRPSQQLRSLN lacZ α c t g g c g t a a t a g c g a a g a g g c c c g c a c c g a t c g c c c t t c c c a a c a g t t g c g c a g c c t g a a g a c c g c a t t a t c g c t t c t c c g g g c g t g g c t a g c g g g a a g g g t t g t c a a c g c g t c g g a c t t

PstIXbaIKasINarISfoIHaeIIPluTI 600 90 95 100 GEWDAPCSGAGL* lacZ α t g g c g a a t g g g a c g c g c c c t g t a g c g g c g c c g g t c t c t a g a g c c a t t t g t c t g c a g a a t t a c c g c t t a c c c t g c g c g g g a c a t c g c c g c g g c c a g a g a t c t c g g t a a a c a g a c g t c t t a a

End (624) 3ʹ 5ʹ 624 g g c g c a c g c g c t a a a a a c g g a c t a c c g c g t g c g c g a t t t t t g c c t g a t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

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Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.