Addgene pCMV G-CEPIA1er Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pCMV G-CEPIA1er Sequencing Result

Map View

800 600 400 200 End (880) PfoI * (868) BssSI - BssSαI (768) BsrFI (738) BtgZI (709) BseRI (618) KpnI (577) BsaWI (576) Acc65I (573) TatI - BsrGI (562) BpmI (537) NmeAIII (527) EGFP-C (506 .. 527) BstBI (477) Bpu10I (456) AvaI - XhoI - BsoBI - PaeR7I (294) SacI - BanII - BsiHKAI (291) Eco53kI (289) BlpI (285) MmeI (230) AccI (226) SalI (225) BamHI (216) PstI (214) BssHII (190) BpuEI (151) HpaI (117) PflMI (66) MscI (56) AseI (43) PmlI - BsaAI (35) Start (0) IgH signal sequence calmodulin-binding peptide IgH signal sequence Kozak sequence pCMV G-CEPIA1er 880 bp

Sequence View

MscIAseIBsaAIPmlIStart (0) 5ʹ 3ʹ 60 Kozak sequence t c t g g c t a a c t a g a g a a c c c a c t g c t t a c t g g c a c g t g g a a a t t a a t a c g a c g t g g c c a c a g a c c g a t t g a t c t c t t g g g t g a c g a a t g a c c g t g c a c c t t t a a t t a t g c t g c a c c g g t g

HpaIPflMI 120 1 5 10 15 MGWSCIILFLVATAT IgH signal sequence 1 5 10 15 MGWSCIILFLVATAT IgH signal sequence Kozak sequence c a t g g g a t g g a g c t g t a t c a t c c t c t t c t t g g t a g c a a c a g c t a c a g g t a a g g g g t t a a c g t a c c c t a c c t c g a c a t a g t a g g a g a a g a a c c a t c g t t g t c g a t g t c c a t t c c c c a a t t g

BpuEI 180 IgH signal sequence IgH signal sequence MGDNDIHFAF a g t a g c a g g c t t g a g g t c t g g a c a t a t a t a t g g g t g a c a a t g a c a t c c a c t t t g c c t t t c t c a t c g t c c g a a c t c c a g a c c t g t a t a t a t a c c c a c t g t t a c t g t a g g t g a a a c g g a a a g

MmeISalIAccIPstIBamHIBssHII 240 GAHS (in frame with IgH signal sequence) QVQLQGSMVDSS 1 R IgH signal sequence calmodulin-binding peptide GAHS (in frame with IgH signal sequence) QVQLQGSMVDSSR IgH signal sequence LSTGAHSQVQLQGSMVDSSR t c t c c a c a g g c g c g c a c t c c c a g g t c c a a c t g c a g g g a t c c a t g g t c g a c t c t t c a c g t c a g a g g t g t c c g c g c g t g a g g g t c c a g g t t g a c g t c c c t a g g t a c c a g c t g a g a a g t g c a g

BlpIEco53kIBsiHKAIBanIISacIPaeR7IBsoBIXhoIAvaI 300 (in frame with IgH signal sequence) RKWNKTGHAVRAIGRLSSLE 5 10 15 RKWNKTGHAVRAIGRLSS LE calmodulin-binding peptide RKWNKTGHAVRAIGRLSSLE *EL g t a a g t g g a a t a a g a c a g g t c a c g c a g t c a g a g c t a t a g g t c g g c t g a g c t c a c t c g a g a c a t t c a c c t t a t t c t g t c c a g t g c g t c a g t c t c g a t a t c c a g c c g a c t c g a g t g a g c t c t

360 (in frame with IgH signal sequence) NVYIKADEQKNGIKAYFKIR (in frame with calmodulin-binding peptide) NVYIKADEQKNGIKAYFKIR NVYIKADEQKNGIKAYFKIR VDIDLGVLLLVADLRVELDA a c g t c t a t a t c a a g g c c g a c g a g c a g a a g a a c g g c a t c a a g g c g t a c t t c a a g a t c c g c c t g c a g a t a t a g t t c c g g c t g c t c g t c t t c t t g c c g t a g t t c c g c a t g a a g t t c t a g g c g g

420 (in frame with IgH signal sequence) HNIEGGGVQLAYHYQQNTPI (in frame with calmodulin-binding peptide) HNIEGGGVQLAYHYQQNTPI HNIEGGGVQLAYHYQQNTPI VVDLAAAHLEGVVVLLVGGD a c a a c a t c g a g g g c g g c g g c g t g c a g c t c g c c t a c c a c t a c c a g c a g a a c a c c c c c a t c g t g t t g t a g c t c c c g c c g c c g c a c g t c g a g c g g a t g g t g a t g g t c g t c t t g t g g g g g t a g c

BstBIBpu10I 480 (in frame with IgH signal sequence) GDGPVLLPDNHYLSVQSILS (in frame with calmodulin-binding peptide) GDGPVLLPDNHYLSVQSILS GDGPVLLPDNHYLSVQSILS AVAGHQQGVVVVQAHLGYKR g c g a c g g c c c c g t g c t g c t g c c c g a c a a c c a c t a c c t g a g c g t g c a g t c c a t a c t t t c g a c g c t g c c g g g g c a c g a c g a c g g g c t g t t g g t g a t g g a c t c g c a c g t c a g g t a t g a a a g c t

BpmINmeAIII 540 (in frame with IgH signal sequence) KDPNEKRDHMVLLEFVTAAG (in frame with calmodulin-binding peptide) KDPNEKRDHMVLLEFVTAAG C A T G G T C C T G C T G G A G T T C G T G EGFP-C KDPNEKRDHMVLLEFVTAAG FVGVLLAIVHDQQLEHGGGP a a g a c c c c a a c g a g a a g c g c g a t c a c a t g g t c c t g c t g g a g t t c g t g a c c g c c g c c g g g a t t c t g g g g t t g c t c t t c g c g c t a g t g t a c c a g g a c g a c c t c a a g c a c t g g c g g c g g c c c t

Acc65IBsaWIKpnIBsrGITatI 600 (in frame with IgH signal sequence) ITLGMDELYKGGTGGSMVSK (in frame with calmodulin-binding peptide) ITLGMDELYKGGTGGSMVSK ITLGMDELYKGGTGGSMVSK DSEAHVLQVLATGSPAHHAL t c a c t c t c g g c a t g g a c g a g c t g t a c a a g g g c g g t a c c g g a g g g a g c a t g g t g a g c a a g g a g t g a g a g c c g t a c c t g c t c g a c a t g t t c c c g c c a t g g c c t c c c t c g t a c c a c t c g t t c c

BseRI 660 (in frame with IgH signal sequence) GEELFTGVVPIQVELDGDVN (in frame with calmodulin-binding peptide) GEELFTGVVPIQVELDGDVN GEELFTGVVPIQVELDGDVN ALLQEGPHHGDLDLQVAVYV g c g a g g a g c t g t t c a c c g g g g t g g t g c c c a t c c a g g t c g a g c t g g a c g g c g a c g t a a a c g c g c t c c t c g a c a a g t g g c c c c a c c a c g g g t a g g t c c a g c t c g a c c t g c c g c t g c a t t t g c

BtgZI 720 (in frame with IgH signal sequence) GHKFSVSGEGEGDATYGKLT (in frame with calmodulin-binding peptide) GHKFSVSGEGEGDATYGKLT GHKFSVSGEGEGDATYGKLT AVLEAHGALTLAIGGVALQG g c c a c a a g t t c a g c g t g t c c g g c g a g g g t g a g g g c g a t g c c a c c t a c g g c a a g c t g a c c c c g g t g t t c a a g t c g c a c a g g c c g c t c c c a c t c c c g c t a c g g t g g a t g c c g t t c g a c t g g g

BssSαIBssSIBsrFI 780 (in frame with IgH signal sequence) LKFICTTGKLPVPWPTLVTT (in frame with calmodulin-binding peptide) LKFICTTGKLPVPWPTLVTT LKFICTTGKLPVPWPTLVTT QLEDAGGALQGHGPGGEHGG t g a a g t t c a t c t g c a c c a c c g g c a a g c t g c c c g t g c c c t g g c c c a c c c t c g t g a c c a c c c a c t t c a a g t a g a c g t g g t g g c c g t t c g a c g g g c a c g g g a c c g g g t g g g a g c a c t g g t g g g

840 (in frame with IgH signal sequence) LTYGVQCFSRYPDHMKQHDF (in frame with calmodulin-binding peptide) LTYGVQCFSRYPDHMKQHDF LTYGVQCFSRYPDHMKQHDF QGVAHLAEAAVGVVHLLVVE t g a c c t a c g g c g t g c a g t g c t t c a g c c g c t a c c c c g a c c a c a t g a a g c a g c a c g a c t t c t a c t g g a t g c c g c a c g t c a c g a a g t c g g c g a t g g g g c t g g t g t a c t t c g t c g t g c t g a a g a

End (880) PfoI* 3ʹ 5ʹ 880 (in frame with IgH signal sequence) FKSAMPEGYIQER (in frame with calmodulin-binding peptide) FKSAMPEGYIQER FKSAMPEGYIQER ELGGHGFAVDLLA t c a a g t c c g c c a t g c c c g a a g g c t a c a t c c a g g a g c g c a c a g t t c a g g c g g t a c g g g c t t c c g a t g t a g g t c c t c g c g t g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.