Addgene mEos2-PTK2-N-10 - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: mEos2-PTK2-N-10


Map View

3000 2000 1000 End (3934) NotI - EagI (3897) NdeI (3687) KpnI (3582) Acc65I (3578) BstBI (3498) EcoRV (3379) BamHI (3196) BspHI (3051) PstI (2629) AlwNI (2624) XhoI PaeR7I (2538) AfeI (2489) CsiI - SexAI * (2244) PfoI * (2055) TaqII (1904) NsiI (1872) PciI AflIII (1382) NaeI (1064) NgoMIV (1062) AleI (911) BpuEI (894) AccI (768) DraI (627) BclI * (499) EcoRI (393) XcmI (372) BsaHI (327) PmlI - BsaAI (316) AclI (100) PvuII - MspA1I (26) BmtI (12) NheI (8) Start (0) Kozak sequence mEos2 mEos2-PTK2-N-10 3934 bp

Sequence View

MspA1IPvuIIBmtINheIStart (0) 5ʹ 3ʹ 60 Kozak sequence RSASATMAAAYLDPHLNHTP c a g a t c c g c t a g c g c c a c c a t g g c a g c t g c t t a c c t t g a c c c c c a c t t g a a t c a c a c a c c g t c t a g g c g a t c g c g g t g g t a c c g t c g a c g a a t g g a a c t g g g g g t g a a c t t a g t g t g t g g

AclI 120 NSSTKTHLGTGMERSPGAME a a a t t c g a g t a c t a a g a c t c a c c t g g g t a c t g g t a t g g a a c g t t c t c c t g g t g c a a t g g a t t t a a g c t c a t g a t t c t g a g t g g a c c c a t g a c c a t a c c t t g c a a g a g g a c c a c g t t a c c t

180 RVLKVFHYFESNSEPTTWAS g c g a g t a t t a a a g g t c t t t c a t t a t t t t g a a a g c a a t a g t g a g c c a a c c a c c t g g g c c a g c g c t c a t a a t t t c c a g a a a g t a a t a a a a c t t t c g t t a t c a c t c g g t t g g t g g a c c c g g t c

240 IIRHGDATDVRGIIQKIVDS t a t t a t c a g g c a t g g a g a t g c t a c t g a t g t c a g g g g c a t c a t t c a g a a g a t a g t g g a c a g a t a a t a g t c c g t a c c t c t a c g a t g a c t a c a g t c c c c g t a g t a a g t c t t c t a t c a c c t g t c

300 HKVKHVACYGFRLSHLRSEE t c a c a a a g t a a a g c a t g t g g c c t g c t a t g g a t t c c g c c t c a g t c a c c t g c g g t c a g a g g a a g t g t t t c a t t t c g t a c a c c g g a c g a t a c c t a a g g c g g a g t c a g t g g a c g c c a g t c t c c t

BsaHIBsaAIPmlI 360 VHWLHVDMGVSSVREKYELA g g t t c a c t g g c t t c a c g t g g a t a t g g g c g t c t c c a g t g t g a g g g a g a a g t a t g a g c t t g c c c a a g t g a c c g a a g t g c a c c t a t a c c c g c a g a g g t c a c a c t c c c t c t t c a t a c t c g a a c g

EcoRIXcmI 420 HPPEEWKYELRIRYLPKGFL t c a c c c a c c a g a g g a g t g g a a a t a t g a a t t g a g a a t t c g t t a t t t g c c a a a a g g a t t t c t a g t g g g t g g t c t c c t c a c c t t t a t a c t t a a c t c t t a a g c a a t a a a c g g t t t t c c t a a a g a

480 NQFTEDKPTLNFFYQQVKSD a a a c c a g t t t a c t g a a g a t a a g c c a a c t t t g a a t t t c t t c t a t c a a c a g g t g a a g a g c g a t t t g g t c a a a t g a c t t c t a t t c g g t t g a a a c t t a a a g a a g a t a g t t g t c c a c t t c t c g c t

BclI* 540 YMLEIADQVDQEIALKLGCL t t a t a t g t t a g a g a t a g c t g a t c a a g t g g a c c a g g a a a t t g c t t t g a a g t t g g g t t g t c t a a t a t a c a a t c t c t a t c g a c t a g t t c a c c t g g t c c t t t a a c g a a a c t t c a a c c c a a c a g a

600 EIRRSYWEMRGNALEKKSNY a g a a a t a c g g c g a t c a t a c t g g g a g a t g c g g g g c a a t g c a c t a g a a a a g a a g t c t a a c t a t c t t t a t g c c g c t a g t a t g a c c c t c t a c g c c c c g t t a c g t g a t c t t t t c t t c a g a t t g a t

DraI 660 EVLEKDVGLKRFFPKSLLDS t g a a g t a t t a g a a a a a g a t g t t g g t t t a a a g c g a t t t t t t c c t a a g a g t t t a c t g g a t t c a c t t c a t a a t c t t t t t c t a c a a c c a a a t t t c g c t a a a a a a g g a t t c t c a a a t g a c c t a a g

720 VKAKTLRKLIQQTFRQFANL t g t c a a g g c c a a a a c a c t a a g a a a a c t g a t c c a a c a a a c a t t t a g a c a a t t t g c c a a c c t a c a g t t c c g g t t t t g t g a t t c t t t t g a c t a g g t t g t t t g t a a a t c t g t t a a a c g g t t g g a

AccI 780 NREESILKFFEILSPVYRFD t a a t a g a g a a g a a a g t a t t c t g a a a t t c t t t g a g a t c c t g t c t c c a g t c t a c a g a t t t g a a t t a t c t c t t c t t t c a t a a g a c t t t a a g a a a c t c t a g g a c a g a g g t c a g a t g t c t a a a c t

840 KECFKCALGSSWIISVELAI t a a g g a a t g c t t c a a g t g t g c t c t t g g t t c a a g c t g g a t t a t t t c a g t g g a a c t g g c a a t a t t c c t t a c g a a g t t c a c a c g a g a a c c a a g t t c g a c c t a a t a a a g t c a c c t t g a c c g t t a

BpuEI 900 GPEEGISYLTDKGCNPTHLA c g g c c c a g a a g a a g g a a t c a g t t a c c t a a c g g a c a a g g g c t g c a a t c c c a c a c a t c t t g c g c c g g g t c t t c t t c c t t a g t c a a t g g a t t g c c t g t t c c c g a c g t t a g g g t g t g t a g a a c g

AleI 960 DFTQVQTIQYSNSEDKDRKG t g a c t t c a c t c a a g t g c a a a c c a t t c a g t a t t c a a a c a g t g a a g a c a a g g a c a g a a a a g g a c t g a a g t g a g t t c a c g t t t g g t a a g t c a t a a g t t t g t c a c t t c t g t t c c t g t c t t t t c c

1020 MLQLKIAGAPEPLTVTAPSL a a t g c t a c a a c t a a a a a t a g c a g g t g c a c c c g a g c c t c t g a c a g t g a c g g c a c c a t c c c t t t a c g a t g t t g a t t t t t a t c g t c c a c g t g g g c t c g g a g a c t g t c a c t g c c g t g g t a g g g a

NgoMIVNaeI 1080 TIAENMADLIDGYCRLVNGT a a c c a t t g c g g a g a a t a t g g c t g a c c t a a t a g a t g g g t a c t g c c g g c t g g t g a a t g g a a c t t g g t a a c g c c t c t t a t a c c g a c t g g a t t a t c t a c c c a t g a c g g c c g a c c a c t t a c c t t g

1140 SQSFIIRPQKEGERALPSIP c t c g c a g t c a t t t a t c a t c a g a c c t c a g a a a g a a g g t g a a c g g g c t t t g c c a t c a a t a c c g a g c g t c a g t a a a t a g t a g t c t g g a g t c t t t c t t c c a c t t g c c c g a a a c g g t a g t t a t g g

1200 KLANSEKQGMRTHAVSVSET a a a g t t g g c c a a c a g c g a a a a g c a a g g c a t g c g g a c a c a c g c c g t c t c t g t g t c a g a a a c t t t c a a c c g g t t g t c g c t t t t c g t t c c g t a c g c c t g t g t g c g g c a g a g a c a c a g t c t t t g

1260 DDYAEIIDEEDTYTMPSTRD a g a t g a t t a t g c t g a g a t t a t a g a t g a a g a a g a t a c t t a c a c c a t g c c c t c a a c c a g g g a t c t a c t a a t a c g a c t c t a a t a t c t a c t t c t t c t a t g a a t g t g g t a c g g g a g t t g g t c c c t

1320 YEIQRERIELGRCIGEGQFG t t a t g a g a t t c a a a g a g a a a g a a t a g a a c t t g g a c g a t g t a t t g g a g a a g g c c a a t t t g g a a t a c t c t a a g t t t c t c t t t c t t a t c t t g a a c c t g c t a c a t a a c c t c t t c c g g t t a a a c c

1380 DVHQGIYMSPENPALAVAIK a g a t g t a c a t c a a g g c a t t t a t a t g a g t c c a g a g a a t c c a g c t t t g g c g g t t g c a a t t a a t c t a c a t g t a g t t c c g t a a a t a t a c t c a g g t c t c t t a g g t c g a a a c c g c c a a c g t t a a t t

AflIIIPciI 1440 TCKNCTSDSVREKFLQEALT a a c a t g t a a a a a c t g t a c t t c g g a c a g c g t g a g a g a g a a a t t t c t t c a a g a a g c c t t a a c t t g t a c a t t t t t g a c a t g a a g c c t g t c g c a c t c t c t c t t t a a a g a a g t t c t t c g g a a t t g

1500 MRQFDHPHIVKLIGVITENP a a t g c g t c a g t t t g a c c a t c c t c a t a t t g t g a a g c t g a t t g g a g t c a t c a c a g a g a a t c c t t a c g c a g t c a a a c t g g t a g g a g t a t a a c a c t t c g a c t a a c c t c a g t a g t g t c t c t t a g g

1560 VWIIMELCTLGELRSFLQVR t g t c t g g a t a a t c a t g g a g c t g t g c a c a c t t g g a g a g c t g a g g t c a t t t t t g c a a g t a a g a c a g a c c t a t t a g t a c c t c g a c a c g t g t g a a c c t c t c g a c t c c a g t a a a a a c g t t c a t t c

1620 KYSLDLASLILYAYQLSTAL g a a a t a c a g t t t g g a t c t a g c a t c t t t g a t c c t g t a t g c c t a t c a g c t t a g t a c a g c t c t c t t t a t g t c a a a c c t a g a t c g t a g a a a c t a g g a c a t a c g g a t a g t c g a a t c a t g t c g a g a

1680 AYLESKRFVHRDIAARNVLV t g c a t a t c t a g a g a g c a a a a g a t t t g t a c a c a g g g a c a t t g c t g c t c g g a a t g t t c t g g t a c g t a t a g a t c t c t c g t t t t c t a a a c a t g t g t c c c t g t a a c g a c g a g c c t t a c a a g a c c a

1740 SSNDCVKLGDFGLSRYMEDS g t c c t c a a a t g a t t g t g t a a a a t t a g g a g a c t t t g g a t t a t c c c g a t a t a t g g a a g a t a g c a g g a g t t t a c t a a c a c a t t t t a a t c c t c t g a a a c c t a a t a g g g c t a t a t a c c t t c t a t c

1800 TYYKASKGKLPIKWMAPESI t a c t t a c t a c a a a g c t t c c a a a g g a a a a t t g c c t a t t a a a t g g a t g g c t c c a g a g t c a a t a t g a a t g a t g t t t c g a a g g t t t c c t t t t a a c g g a t a a t t t a c c t a c c g a g g t c t c a g t t a

1860 NFRRFTSASDVWMFGVCMWE c a a t t t t c g a c g t t t t a c c t c a g c t a g t g a c g t a t g g a t g t t t g g t g t g t g t a t g t g g g a g t t a a a a g c t g c a a a a t g g a g t c g a t c a c t g c a t a c c t a c a a a c c a c a c a c a t a c a c c c t

TaqIINsiI 1920 ILMHGVKPFQGVKNNDVIGR g a t a c t g a t g c a t g g t g t g a a g c c t t t t c a a g g a g t g a a g a a c a a t g a t g t a a t c g g t c g c t a t g a c t a c g t a c c a c a c t t c g g a a a a g t t c c t c a c t t c t t g t t a c t a c a t t a g c c a g c

1980 IENGERLPMPPNCPPTLYSL a a t t g a a a a t g g g g a a a g a t t a c c a a t g c c t c c a a a t t g t c c t c c t a c c c t c t a c a g c c t t t a a c t t t t a c c c c t t t c t a a t g g t t a c g g a g g t t t a a c a g g a g g a t g g g a g a t g t c g g a

2040 MTKCWAYDPSRRPRFTELKA t a t g a c g a a a t g c t g g g c c t a t g a c c c c a g c a g g c g g c c c a g g t t t a c t g a a c t t a a a g c a t a c t g c t t t a c g a c c c g g a t a c t g g g g t c g t c c g c c g g g t c c a a a t g a c t t g a a t t t c g

PfoI* 2100 QLSTILEEEKAQQEERMRME t c a g c t c a g c a c a a t c c t g g a g g a a g a g a a g g c t c a g c a a g a a g a g c g c a t g a g g a t g g a a g t c g a g t c g t g t t a g g a c c t c c t t c t c t t c c g a g t c g t t c t t c t c g c g t a c t c c t a c c t

2160 SRRQATVSWDSGGSDEAPPK g t c c a g a a g a c a g g c c a c a g t g t c c t g g g a c t c c g g a g g g t c t g a t g a a g c a c c g c c c a a c a g g t c t t c t g t c c g g t g t c a c a g g a c c c t g a g g c c t c c c a g a c t a c t t c g t g g c g g g t t

2220 PSRPGYPSPRSSEGFYPSPQ g c c c a g c a g a c c g g g t t a t c c c a g t c c g a g g t c c a g c g a a g g a t t t t a t c c c a g c c c a c a c g g g t c g t c t g g c c c a a t a g g g t c a g g c t c c a g g t c g c t t c c t a a a a t a g g g t c g g g t g t

SexAI*CsiI 2280 HMVQTNHYQVSGYPGSHGIT g c a c a t g g t a c a a a c c a a t c a t t a c c a g g t t t c t g g c t a c c c t g g t t c a c a t g g a a t c a c c g t g t a c c a t g t t t g g t t a g t a a t g g t c c a a a g a c c g a t g g g a c c a a g t g t a c c t t a g t g

2340 AMAGSIYPGQASLLDQTDSW a g c c a t g g c t g g c a g c a t c t a t c c a g g t c a g g c a t c t c t t t t g g a c c a a a c a g a t t c a t g t c g g t a c c g a c c g t c g t a g a t a g g t c c a g t c c g t a g a g a a a a c c t g g t t t g t c t a a g t a c

2400 NHRPQEIAMWQPNVEDSTVL g a a t c a t a g a c c t c a g g a g a t a g c a a t g t g g c a g c c c a a t g t g g a g g a c t c t a c a g t a t t c t t a g t a t c t g g a g t c c t c t a t c g t t a c a c c g t c g g g t t a c a c c t c c t g a g a t g t c a t a a

2460 DLRGIGQVLPTHLMEERLIR g g a c c t g c g a g g g a t t g g g c a a g t g t t g c c a a c c c a t c t g a t g g a a g a g c g t c t a a t c c g c c t g g a c g c t c c c t a a c c c g t t c a c a a c g g t t g g g t a g a c t a c c t t c t c g c a g a t t a g g c

AfeI 2520 QQQEMEEDQRWLEKEERFLK a c a g c a a c a g g a a a t g g a a g a a g a t c a g c g c t g g c t g g a a a a a g a g g a a a g a t t t c t g a a t g t c g t t g t c c t t t a c c t t c t t c t a g t c g c g a c c g a c c t t t t t c t c c t t t c t a a a g a c t t

PaeR7IXhoI 2580 PDVRLSRGSIDREDGSLQGP a c c t g a t g t g a g a c t c t c t c g a g g c a g t a t t g a c a g g g a g g a t g g a a g t c t t c a g g g t c c t g g a c t a c a c t c t g a g a g a g c t c c g t c a t a a c t g t c c c t c c t a c c t t c a g a a g t c c c a g g

PstIAlwNI 2640 IGNQHIYQPVGKPDPAAPPK g a t t g g a a a c c a a c a t a t a t a t c a g c c t g t g g g t a a a c c a g a t c c t g c a g c t c c a c c a a a c t a a c c t t t g g t t g t a t a t a t a g t c g g a c a c c c a t t t g g t c t a g g a c g t c g a g g t g g t t t

2700 KPPRPGAPGHLGSLASLSSP g a a a c c g c c t c g c c c t g g a g c t c c c g g t c a t c t g g g a a g c c t t g c c a g c c t c a g c a g c c c c t t t g g c g g a g c g g g a c c t c g a g g g c c a g t a g a c c c t t c g g a a c g g t c g g a g t c g t c g g g

2760 ADSYNEGVKPWRLQPQEISP t g c t g a c a g c t a c a a c g a g g g t g t c a a g c c a t g g a g g c t t c a g c c c c a g g a a a t c a g c c c a c g a c t g t c g a t g t t g c t c c c a c a g t t c g g t a c c t c c g a a g t c g g g g t c c t t t a g t c g g g

2820 PPTANLDRSNDKVYENVTGL c c c t c c t a c t g c c a a c c t g g a c c g g t c g a a t g a t a a g g t g t a c g a g a a t g t g a c g g g c c t g g g a g g a t g a c g g t t g g a c c t g g c c a g c t t a c t a t t c c a c a t g c t c t t a c a c t g c c c g g a

2880 VKAVIEMSSKIQPAPPEEYV g g t g a a a g c t g t c a t c g a g a t g t c c a g t a a a a t c c a g c c a g c c c c a c c a g a g g a g t a t g t c c a c t t t c g a c a g t a g c t c t a c a g g t c a t t t t a g g t c g g t c g g g g t g g t c t c c t c a t a c a

2940 PMVKEVGLALRTLLATVDET c c c t a t g g t g a a g g a a g t c g g c t t g g c c c t g a g g a c a t t a t t g g c c a c t g t g g a t g a g a c g g g a t a c c a c t t c c t t c a g c c g a a c c g g g a c t c c t g t a a t a a c c g g t g a c a c c t a c t c t g

3000 IPLLPASTHREIEMAQKLLN c a t t c c c c t c c t a c c a g c c a g c a c c c a c c g a g a g a t t g a g a t g g c a c a g a a g c t a t t g a a g t a a g g g g a g g a t g g t c g g t c g t g g g t g g c t c t c t a a c t c t a c c g t g t c t t c g a t a a c t t

BspHI 3060 SDLGELINKMKLAQQYVMTS c t c t g a c c t g g g t g a g c t c a t c a a c a a g a t g a a a c t g g c c c a g c a g t a t g t c a t g a c c a g g a g a c t g g a c c c a c t c g a g t a g t t g t t c t a c t t t g a c c g g g t c g t c a t a c a g t a c t g g t c

3120 LQQEYKKQMLTAAHALAVDA c c t c c a g c a a g a g t a c a a a a a g c a a a t g c t g a c t g c t g c t c a c g c c c t g g c t g t g g a t g c g g a g g t c g t t c t c a t g t t t t t c g t t t a c g a c t g a c g a c g a g t g c g g g a c c g a c a c c t a c g

3180 KNLLDVIDQARLKMLGQTRP *EIDNVLCSQFHKPLRSW c a a a a a c t t a c t c g a t g t c a t t g a c c a a g c a a g a c t g a a a a t g c t t g g g c a g a c g a g a c c g t t t t t g a a t g a g c t a c a g t a a c t g g t t c g t t c t g a c t t t t a c g a a c c c g t c t g c t c t g g

BamHI 3240 1 5 MSAIKPDMK mEos2 HSSGGDPPVATMSAIKPDMK VAGSPIWRDGGHTRNLWVHL a c a c a g c t c c g g a g g g g a t c c a c c g g t c g c c a c c a t g a g t g c g a t t a a g c c a g a c a t g a a t g t g t c g a g g c c t c c c c t a g g t g g c c a g c g g t g g t a c t c a c g c t a a t t c g g t c t g t a c t t

3300 10 15 20 25 IKLRMEGNVNGHHFVIDGDG mEos2 IKLRMEGNVNGHHFVIDGDG DFETHFAVYVPVVKHDVSIT g a t c a a a c t c c g t a t g g a a g g c a a c g t a a a c g g g c a c c a c t t t g t g a t c g a c g g a g a t g g c t a g t t t g a g g c a t a c c t t c c g t t g c a t t t g c c c g t g g t g a a a c a c t a g c t g c c t c t a c c

3360 30 35 40 45 TGKPFEGKQSMDLEVKEGGP mEos2 TGKPFEGKQSMDLEVKEGGP CALRKLSFLTHIKFDFLASR t a c a g g c a a g c c t t t t g a g g g a a a a c a g a g t a t g g a t c t t g a a g t c a a a g a g g g c g g a c c a t g t c c g t t c g g a a a a c t c c c t t t t g t c t c a t a c c t a g a a c t t c a g t t t c t c c c g c c t g g

EcoRV 3420 50 55 60 65 LPFAFDILTTAFHYGNRVFA mEos2 LPFAFDILTTAFHYGNRVFA QRKGKIDQGSCEMVAVPYEG t c t g c c t t t t g c c t t t g a t a t c c t g a c c a c t g c a t t c c a t t a c g g c a a c a g g g t a t t c g c a g a c g g a a a a c g g a a a c t a t a g g a c t g g t g a c g t a a g g t a a t g c c g t t g t c c c a t a a g c g

3480 70 75 80 85 KYPDNIQDYFKQSFPKGYSW mEos2 KYPDNIQDYFKQSFPKGYSW FIWVVYLVIKLLRKRLPIRP c a a a t a t c c a g a c a a c a t a c a a g a c t a t t t t a a g c a g t c g t t t c c t a a g g g g t a t t c g t g g t t t a t a g g t c t g t t g t a t g t t c t g a t a a a a t t c g t c a g c a a a g g a t t c c c c a t a a g c a c

BstBI 3540 90 95 100 105 ERSLTFEDGGICIARNDITM mEos2 ERSLTFEDGGICIARNDITM FSAQSEFVPANANGSVVYCH g g a a c g a a g c t t g a c t t t c g a a g a c g g g g g c a t t t g c a t t g c c a g a a a c g a c a t a a c a a t c c t t g c t t c g a a c t g a a a g c t t c t g c c c c c g t a a a c g t a a c g g t c t t t g c t g t a t t g t t a

Acc65IKpnI 3600 110 115 120 125 EGDTFYNKVRFYGTNFPANG mEos2 EGDTFYNKVRFYGTNFPANG FPVSEIIFNSKITGVKGGIT g g a a g g g g a c a c t t t c t a t a a t a a a g t t c g a t t t t a t g g t a c c a a c t t t c c c g c c a a t g g c c t t c c c c t g t g a a a g a t a t t a t t t c a a g c t a a a a t a c c a t g g t t g a a a g g g c g g t t a c c

3660 130 135 140 145 PVMQKKTLKWEPSTEKMYVR mEos2 PVMQKKTLKWEPSTEKMYVR WNHLLLRQFPLGGSLFHIHT t c c a g t t a t g c a g a a g a a g a c g c t g a a a t g g g a g c c c t c c a c t g a g a a a a t g t a t g t g c g a g g t c a a t a c g t c t t c t t c t g c g a c t t t a c c c t c g g g a g g t g a c t c t t t t a c a t a c a c g c

NdeI 3720 150 155 160 165 DGVLTGDIHMALLLEGNAHY mEos2 DGVLTGDIHMALLLEGNAHY ISHQRTINMHSQQKFSIGM t g a t g g a g t g c t g a c g g g t g a t a t t c a t a t g g c t t t g t t g c t t g a a g g a a a t g c c c a t t a a c t a c c t c a c g a c t g c c c a c t a t a a g t a t a c c g a a a c a a c g a a c t t c c t t t a c g g g t a a t

3780 170 175 180 185 RCDFRTTYKAKEKGVKLPGY mEos2 RCDFRTTYKAKEKGVKLPGY c c g a t g t g a c t t c a g a a c t a c t t a c a a a g c t a a g g a g a a g g g t g t c a a g t t a c c a g g c t a g g c t a c a c t g a a g t c t t g a t g a a t g t t t c g a t t c c t c t t c c c a c a g t t c a a t g g t c c g a t

3840 190 195 200 205 HFVDHCIEILSHDKDYNKVK mEos2 HFVDHCIEILSHDKDYNKVK c c a c t t t g t g g a c c a c t g c a t t g a g a t t t t a a g c c a t g a c a a a g a t t a c a a c a a g g t t a a g g t g a a a c a c c t g g t g a c g t a a c t c t a a a a t t c g g t a c t g t t t c t a a t g t t g t t c c a a t t

EagINotI 3900 210 215 220 225 LYEHAVAHSGLPDNARR* mEos2 LYEHAVAHSGLPDNARR* g c t g t a t g a g c a t g c t g t t g c t c a t t c t g g a t t g c c t g a c a a t g c c a g a c g a t a a g c g g c c g a c a t a c t c g t a c g a c a a c g a g t a a g a c c t a a c g g a c t g t t a c g g t c t g c t a t t c g c c g

End (3934) 3ʹ 5ʹ 3934 c g c g a c t c t a g a t c a t a a t c a g c c a t a c c a c a t t g c g c t g a g a t c t a g t a t t a g t c g g t a t g g t g t a a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.