Addgene sfGFP-Zyxin-6 - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: sfGFP-Zyxin-6


Map View

2500 2000 1500 1000 500 End (2538) BsaBI * (2519) XbaI * (2511) NotI (2501) BsrGI (2489) EGFP-C (2433 .. 2454) BlpI (2401) BmrI (2385) PflMI (2292) BssSI - BssSαI (1960) EGFP-N (1825 .. 1846) AgeI (1767) BamHI (1761) PmlI BsaAI (1722) StuI (1429) BbvCI (1411) NspI - SphI (1410) PluTI (1336) SfoI (1334) NarI - BsaHI (1333) KasI (1332) FspI (1292) SrfI (1222) HincII (1185) CsiI - SexAI * (1064) TsoI (775) AlwNI (669) PstI (615) MscI (577) AhdI (495) BspEI (405) PvuI (344) BbsI (257) BssHII (204) BsaI (90) TaqII (72) Esp3I - BsmBI (68) ApoI - EcoRI (39) BstBI (37) HindIII (32) SacI (30) Eco53kI (28) XhoI - PaeR7I (23) BpuEI (14) AfeI (6) BmtI (5) NheI (1) Start (0) Kozak sequence superfolder GFP Kozak sequence sfGFP-Zyxin-6 2538 bp

Sequence View

PaeR7IXhoIEco53kISacIHindIIIBstBIEcoRIApoIBpuEIStart (0) NheIBmtIAfeI 60 Kozak sequence ASATGLRSRAQASNSMAAPR g c t a g c g c t a c c g g a c t c a g a t c t c g a g c t c a a g c t t c g a a t t c c a t g g c g g c c c c c c g c c g a t c g c g a t g g c c t g a g t c t a g a g c t c g a g t t c g a a g c t t a a g g t a c c g c c g g g g g g c g

BsaIBsmBIEsp3ITaqII 120 PSPAIPVSVSAPAFYAPQKK c c g t c t c c c g c g a t c c c c g t t t c g g t c t c g g c t c c g g c t t t t t a c g c c c c g c a g a a g a a g g g c a g a g g g c g c t a g g g g c a a a g c c a g a g c c g a g g c c g a a a a a t g c g g g g c g t c t t c t t c

180 FGPVVAPKPKVNPFRPRDSE t t c g g c c c t g t g g t g g c c c c a a a g c c c a a a g t g a a t c c c t t c c g g c c c a g g g a c a g c g a g a a g c c g g g a c a c c a c c g g g g t t t c g g g t t t c a c t t a g g g a a g g c c g g g t c c c t g t c g c t c

BssHII 240 PPPAPGAQRAQMGRVGDIPP c c t c c c c c g g c a c c c g g g g c c c a g c g c g c a c a g a t g g g c c g g g t g g g c g a t a t t c c c c c g g g a g g g g g c c g t g g g c c c c g g g t c g c g c g t g t c t a c c c g g c c c a c c c g c t a t a a g g g g g c

BbsI 300 PPPEDFPLPPPPLAGDGDDA c c g c c c c c g g a a g a c t t t c c c c t g c c t c c a c c t c c c c t t g c t g g g g a t g g c g a c g a t g c a g g c g g g g g c c t t c t g a a a g g g g a c g g a g g t g g a g g g g a a c g a c c c c t a c c g c t g c t a c g t

PvuI 360 EGALGGAFPPPPPPIEESFP g a g g g t g c t c t g g g a g g t g c c t t c c c g c c g c c c c c t c c c c c g a t c g a g g a a t c a t t t c c c c t c c c a c g a g a c c c t c c a c g g a a g g g c g g c g g g g g a g g g g g c t a g c t c c t t a g t a a a g g g

BspEI 420 PAPLEEEIFPSPPPPPEEEG c c t g c g c c t c t g g a g g a g g a g a t c t t c c c t t c c c c g c c g c c t c c t c c g g a g g a g g a g g g a g g a c g c g g a g a c c t c c t c c t c t a g a a g g g a a g g g g c g g c g g a g g a g g c c t c c t c c t c c c t

480 GPEAPIPPPPQPREKVSSID g g g c c t g a g g c c c c c a t a c c g c c c c c a c c a c a g c c c a g g g a g a a g g t g a g c a g t a t t g a t c c c g g a c t c c g g g g g t a t g g c g g g g g t g g t g t c g g g t c c c t c t t c c a c t c g t c a t a a c t a

AhdI 540 LEIDSLSSLLDDMTKNDPFK t t g g a g a t c g a c t c t c t g t c c t c a c t g c t g g a t g a c a t g a c c a a g a a t g a t c c t t t c a a a a a c c t c t a g c t g a g a g a c a g g a g t g a c g a c c t a c t g t a c t g g t t c t t a c t a g g a a a g t t t

MscI 600 ARVSSGYVPPPVATPFSSKS g c c c g g g t g t c a t c t g g a t a t g t g c c c c c a c c a g t g g c c a c t c c a t t c a g t t c c a a g t c c c g g g c c c a c a g t a g a c c t a t a c a c g g g g g t g g t c a c c g g t g a g g t a a g t c a a g g t t c a g g

PstI 660 STKPAAGGTAPLPPWKSPSS a g t a c c a a g c c t g c a g c c g g g g g c a c a g c a c c c c t g c c t c c t t g g a a g t c c c c t t c c a g c t c a t g g t t c g g a c g t c g g c c c c c g t g t c g t g g g g a c g g a g g a a c c t t c a g g g g a a g g t c g

AlwNI 720 SQPLPQVPAPAQSQTQFHVQ t c c c a g c c t c t g c c c c a g g t t c c g g c t c c g g c t c a g a g c c a g a c a c a g t t c c a t g t t c a g a g g g t c g g a g a c g g g g t c c a a g g c c g a g g c c g a g t c t c g g t c t g t g t c a a g g t a c a a g t c

TsoI 780 PQPQPKPQVQLHVQSQTQPV c c c c a g c c c c a g c c c a a g c c t c a g g t c c a a c t c c a t g t c c a g t c c c a g a c c c a g c c t g t g g g g g t c g g g g t c g g g t t c g g a g t c c a g g t t g a g g t a c a g g t c a g g g t c t g g g t c g g a c a c

840 SLANTQPRGPPASSPAPAPK t c t t t g g c t a a c a c c c a g c c c c g a g g g c c c c c a g c c t c a t c t c c g g c t c c a g c c c c t a a g a g a a a c c g a t t g t g g g t c g g g g c t c c c g g g g g t c g g a g t a g a g g c c g a g g t c g g g g a t t c

900 FSPVTPKFTPVASKFSPGAP t t t t c t c c a g t g a c t c c t a a g t t t a c t c c t g t g g c t t c c a a g t t c a g t c c t g g a g c c c c a a a a a g a g g t c a c t g a g g a t t c a a a t g a g g a c a c c g a a g g t t c a a g t c a g g a c c t c g g g g t

960 GGSGSQPNQKLGHPEALSAG g g t g g a t c t g g g t c a c a a c c a a a t c a a a a a t t g g g g c a c c c c g a a g c t c t t t c t g c t g g c c c a c c t a g a c c c a g t g t t g g t t t a g t t t t t a a c c c c g t g g g g c t t c g a g a a a g a c g a c c g

1020 TGSPQPPSFTYAQQREKPRV a c a g g c t c c c c t c a a c c t c c c a g c t t c a c c t a t g c c c a g c a g a g g g a g a a g c c c c g a g t g t g t c c g a g g g g a g t t g g a g g g t c g a a g t g g a t a c g g g t c g t c t c c c t c t t c g g g g c t c a c

SexAI*CsiI 1080 QEKQHPVPPPAQNQNQVRSP c a g g a g a a g c a g c a c c c c g t g c c c c c a c c g g c t c a g a a c c a a a a c c a g g t g c g c t c c c c t g t c c t c t t c g t c g t g g g g c a c g g g g g t g g c c g a g t c t t g g t t t t g g t c c a c g c g a g g g g a

1140 GAPGPLTLKEVEELEQLTQQ g g g g c c c c a g g g c c c c t g a c t c t g a a g g a g g t g g a g g a g c t g g a g c a g c t g a c c c a g c a g c c c c g g g g t c c c g g g g a c t g a g a c t t c c t c c a c c t c c t c g a c c t c g t c g a c t g g g t c g t c

HincII 1200 LMQDMEHPQRQNVAVNELCG c t a a t g c a g g a c a t g g a g c a t c c t c a g a g g c a g a a t g t g g c t g t c a a c g a a c t c t g c g g c g a t t a c g t c c t g t a c c t c g t a g g a g t c t c c g t c t t a c a c c g a c a g t t g c t t g a g a c g c c g

SrfI 1260 RCHQPLARAQPAVRALGQLF c g a t g c c a t c a a c c c c t g g c c c g g g c g c a g c c a g c c g t c c g c g c t c t a g g g c a g c t g t t c g c t a c g g t a g t t g g g g a c c g g g c c c g c g t c g g t c g g c a g g c g c g a g a t c c c g t c g a c a a g

FspI 1320 HIACFTCHQCAQQLQGQQFY c a c a t c g c c t g c t t c a c c t g c c a c c a g t g t g c g c a g c a g c t c c a g g g c c a g c a g t t c t a c g t g t a g c g g a c g a a g t g g a c g g t g g t c a c a c g c g t c g t c g a g g t c c c g g t c g t c a a g a t g

KasIBsaHINarISfoIPluTI 1380 SLEGAPYCEGCYTDTLEKCN a g t c t g g a g g g g g c g c c g t a c t g c g a g g g c t g t t a c a c t g a c a c c c t g g a g a a g t g t a a c t c a g a c c t c c c c c g c g g c a t g a c g c t c c c g a c a a t g t g a c t g t g g g a c c t c t t c a c a t t g

StuISphINspIBbvCI 1440 TCGEPITDRMLRATGKAYHP a c c t g c g g g g a g c c c a t c a c t g a c c g c a t g c t g a g g g c c a c g g g c a a g g c c t a t c a c c c g t g g a c g c c c c t c g g g t a g t g a c t g g c g t a c g a c t c c c g g t g c c c g t t c c g g a t a g t g g g c

1500 HCFTCVVCARPLEGTSFIVD c a c t g c t t c a c c t g t g t g g t c t g c g c c c g c c c c c t g g a g g g c a c c t c c t t c a t c g t g g a c g t g a c g a a g t g g a c a c a c c a g a c g c g g g c g g g g g a c c t c c c g t g g a g g a a g t a g c a c c t g

1560 QANRPHCVPDYHKQYAPRCS c a g g c c a a c c g g c c c c a c t g t g t c c c c g a c t a c c a c a a g c a g t a c g c c c c g a g g t g c t c c g t c c g g t t g g c c g g g g t g a c a c a g g g g c t g a t g g t g t t c g t c a t g c g g g g c t c c a c g a g g

1620 VCSEPIMPEPGRDETVRVVA g t c t g c t c t g a g c c c a t c a t g c c t g a g c c t g g c c g a g a t g a g a c t g t g c g a g t g g t c g c c c a g a c g a g a c t c g g g t a g t a c g g a c t c g g a c c g g c t c t a c t c t g a c a c g c t c a c c a g c g g

1680 LDKNFHMKCYKCEDCGKPLS c t g g a c a a g a a c t t c c a c a t g a a g t g t t a c a a g t g t g a g g a c t g c g g g a a g c c c c t g t c g g a c c t g t t c t t g a a g g t g t a c t t c a c a a t g t t c a c a c t c c t g a c g c c c t t c g g g g a c a g c

BsaAIPmlI 1740 IEADDNGCFPLDGHVLCRKC a t t g a g g c a g a t g a c a a t g g c t g c t t c c c c c t g g a c g g t c a c g t g c t c t g t c g g a a g t g c t a a c t c c g t c t a c t g t t a c c g a c g a a g g g g g a c c t g c c a g t g c a c g a g a c a g c c t t c a c g

AgeIBamHI 1800 1 M 1a V 5 SKGEE superfolder GFP Kozak sequence HTARAQTDPPVATMVSKGEE c a c a c t g c t a g a g c c c a g a c g g a t c c a c c g g t c g c c a c c a t g g t g a g c a a g g g c g a g g a g g t g t g a c g a t c t c g g g t c t g c c t a g g t g g c c a g c g g t g g t a c c a c t c g t t c c c g c t c c t c

1860 10 15 20 25 LFTGVVPILVELDGDVNGHK superfolder GFP G A C C A G C T C G A C C T G C C G C T G C EGFP-N LFTGVVPILVELDGDVNGHK c t g t t c a c c g g g g t g g t g c c c a t c c t g g t c g a g c t g g a c g g c g a c g t a a a c g g c c a c a a g g a c a a g t g g c c c c a c c a c g g g t a g g a c c a g c t c g a c c t g c c g c t g c a t t t g c c g g t g t t c

1920 30 35 40 45 FSVRGEGEGDATNGKLTLKF superfolder GFP FSVRGEGEGDATNGKLTLKF t t c a g c g t g c g c g g c g a g g g c g a g g g c g a t g c c a c c a a c g g c a a g c t g a c c c t g a a g t t c a a g t c g c a c g c g c c g c t c c c g c t c c c g c t a c g g t g g t t g c c g t t c g a c t g g g a c t t c a a g

BssSαIBssSI 1980 50 55 60 65 ICTTGKLPVPWPTLVTTLTY superfolder GFP ICTTGKLPVPWPTLVTTLTY a t c t g c a c c a c c g g c a a g c t g c c c g t g c c c t g g c c c a c c c t c g t g a c c a c c c t g a c c t a c t a g a c g t g g t g g c c g t t c g a c g g g c a c g g g a c c g g g t g g g a g c a c t g g t g g g a c t g g a t g

2040 70 75 80 85 GVQCFSRYPDHMKRHDFFKS superfolder GFP GVQCFSRYPDHMKRHDFFKS g g c g t g c a g t g c t t c a g c c g c t a c c c c g a c c a c a t g a a g c g c c a c g a c t t c t t c a a g t c c c c g c a c g t c a c g a a g t c g g c g a t g g g g c t g g t g t a c t t c g c g g t g c t g a a g a a g t t c a g g

2100 90 95 100 105 AMPEGYVQERTISFKDDGTY superfolder GFP AMPEGYVQERTISFKDDGTY g c c a t g c c c g a a g g c t a c g t c c a g g a g c g c a c c a t c a g c t t c a a g g a c g a c g g c a c c t a c c g g t a c g g g c t t c c g a t g c a g g t c c t c g c g t g g t a g t c g a a g t t c c t g c t g c c g t g g a t g

2160 110 115 120 125 KTRAEVKFEGDTLVNRIELK superfolder GFP KTRAEVKFEGDTLVNRIELK a a g a c c c g c g c c g a g g t g a a g t t c g a g g g c g a c a c c c t g g t g a a c c g c a t c g a g c t g a a g t t c t g g g c g c g g c t c c a c t t c a a g c t c c c g c t g t g g g a c c a c t t g g c g t a g c t c g a c t t c

2220 130 135 140 145 GIDFKEDGNILGHKLEYNFN superfolder GFP GIDFKEDGNILGHKLEYNFN g g c a t c g a c t t c a a g g a g g a c g g c a a c a t c c t g g g g c a c a a g c t g g a g t a c a a c t t c a a c c c g t a g c t g a a g t t c c t c c t g c c g t t g t a g g a c c c c g t g t t c g a c c t c a t g t t g a a g t t g

2280 150 155 160 165 SHNVYITADKQKNGIKANFK superfolder GFP SHNVYITADKQKNGIKANFK a g c c a c a a c g t c t a t a t c a c c g c c g a c a a g c a g a a g a a c g g c a t c a a g g c c a a c t t c a a g t c g g t g t t g c a g a t a t a g t g g c g g c t g t t c g t c t t c t t g c c g t a g t t c c g g t t g a a g t t c

PflMI 2340 170 175 180 185 IRHNVEDGSVQLADHYQQNT superfolder GFP IRHNVEDGSVQLADHYQQNT a t c c g c c a c a a c g t g g a g g a c g g c a g c g t g c a g c t c g c c g a c c a c t a c c a g c a g a a c a c c t a g g c g g t g t t g c a c c t c c t g c c g t c g c a c g t c g a g c g g c t g g t g a t g g t c g t c t t g t g g

BmrI 2400 190 195 200 205 PIGDGPVLLPDNHYLSTQSV superfolder GFP PIGDGPVLLPDNHYLSTQSV c c c a t c g g c g a c g g c c c c g t g c t g c t g c c c g a c a a c c a c t a c c t g a g c a c c c a g t c c g t g g g g t a g c c g c t g c c g g g g c a c g a c g a c g g g c t g t t g g t g a t g g a c t c g t g g g t c a g g c a c

BlpI 2460 210 215 220 225 LSKDPNEKRDHMVLLEFVTA superfolder GFP C A T G G T C C T G C T G G A G T T C G T G EGFP-C LSKDPNEKRDHMVLLEFVTA c t g a g c a a a g a c c c c a a c g a g a a g c g c g a t c a c a t g g t c c t g c t g g a g t t c g t g a c c g c c g a c t c g t t t c t g g g g t t g c t c t t c g c g c t a g t g t a c c a g g a c g a c c t c a a g c a c t g g c g g

BsaBI*XbaI*NotIBsrGI 2520 230 235 AGITHGMDELYK* superfolder GFP AGITHGMDELYK* g c c g g g a t c a c t c a c g g c a t g g a c g a g c t g t a c a a g t a a g c g g c c g c g a c t c t a g a t c a t c g g c c c t a g t g a g t g c c g t a c c t g c t c g a c a t g t t c a t t c g c c g g c g c t g a g a t c t a g t a

End (2538) 2538 a a t c a g c c a t a c c a c a t t t t a g t c g g t a t g g t g t a a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.