Addgene mRuby-Clathrin-15 - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: mRuby-Clathrin-15


Map View

1250 1000 750 500 250 End (1443) BsaBI * (1424) XbaI * (1409) BamHI (1397) DrdI (1384) BtsI - BtsαI (1334) BsaI (1256) BpmI (1187) BsrDI (1026) TsoI (1005) PshAI (971) BssSI - BssSαI (937) ApaI (935) PspOMI (931) BfuAI - PaqCI - BspMI (907) NotI - EagI (831) HincII (757) AccI (756) SalI (755) EcoRI (745) BstBI (743) HindIII (738) SacI (736) Eco53kI (734) XhoI - PaeR7I (729) BglII (725) BpuEI (720) BspEI (716) BsgI (689) EarI (636) Bpu10I (542) PluTI (426) SfoI (424) NarI (423) KasI (422) SgrAI (113) ApaLI (109) NspI (61) AgeI (22) AfeI (18) BmtI (17) NheI (13) AatII (5) ZraI (3) Start (0) mRuby mRuby-Clathrin-15 1443 bp

Sequence View

AgeINheIBmtIAfeIStart (0) ZraIAatII 60 1 5 MNSLIKENM mRuby DVRSASATGRHHEQPDQRKH TSDPLALPVATMNSLIKENM *RDGGHVAQDFFVH g a c g t c a g a t c c g c t a g c g c t a c c g g t c g c c a c c a t g a a c a g c c t g a t c a a a g a a a a c a t c t g c a g t c t a g g c g a t c g c g a t g g c c a g c g g t g g t a c t t g t c g g a c t a g t t t c t t t t g t a

ApaLISgrAINspI 120 10 15 20 25 RMKVVLEGSVNGHQFKCTGE mRuby ADEGGAGRQRERPPVQVHRR RMKVVLEGSVNGHQFKCTGE PHLHHQFAAHVAVLELAGAL g c g g a t g a a g g t g g t g c t g g a a g g c a g c g t g a a c g g c c a c c a g t t c a a g t g c a c c g g c g a c g c c t a c t t c c a c c a c g a c c t t c c g t c g c a c t t g c c g g t g g t c a a g t t c a c g t g g c c g c t

180 30 35 40 45 GEGNPYMGTQTMRIKVIEGG mRuby GRGQPLHGHPDHADQSDRGR GEGNPYMGTQTMRIKVIEGG ALAVGVHAGLGHPDFHDLAS g g g c g a g g g c a a c c c c t a c a t g g g c a c c c a g a c c a t g c g g a t c a a a g t g a t c g a g g g c g g c c c g c t c c c g t t g g g g a t g t a c c c g t g g g t c t g g t a c g c c t a g t t t c a c t a g c t c c c g c c

240 50 55 60 65 PLPFAFDILATSFMYGSRTF mRuby TSALRLRHPGHILHVRQPDL PLPFAFDILATSFMYGSRTF RQGEGEVDQGCGEHVAAPGE a c c t c t g c c c t t c g c c t t c g a c a t c c t g g c c a c a t c c t t c a t g t a c g g c a g c c g g a c c t t t g g a g a c g g g a a g c g g a a g c t g t a g g a c c g g t g t a g g a a g t a c a t g c c g t c g g c c t g g a a

300 70 75 80 85 IKYPKGIPDFFKQSFPEGFT mRuby HQVPQGHPRFLQAELPRGLH IKYPKGIPDFFKQSFPEGFT DLVGLADGIEELLAEGLAEG c a t c a a g t a c c c c a a g g g c a t c c c c g a t t t c t t c a a g c a g a g c t t c c c c g a g g g c t t c a c g t a g t t c a t g g g g t t c c c g t a g g g g c t a a a g a a g t t c g t c t c g a a g g g g c t c c c g a a g t g

360 90 95 100 105 WERVTRYEDGGVITVMQDTS mRuby LGESDQIRGRRRDHRDAGHQ WERVTRYEDGGVITVMQDTS PLSHGSVLVAAHDGHHLVGA c t g g g a g a g a g t g a c c a g a t a c g a g g a c g g c g g c g t g a t c a c c g t g a t g c a g g a c a c c a g g a c c c t c t c t c a c t g g t c t a t g c t c c t g c c g c c g c a c t a g t g g c a c t a c g t c c t g t g g t c

420 110 115 120 125 LEDGCLVYHAQVRGVNFPSN mRuby PGRWLPGVPCPGQGREFSQQ LEDGCLVYHAQVRGVNFPSN QFIAAQHVMGLDPAHIKGAV c c t g g a a g a t g g c t g c c t g g t g t a c c a t g c c c a g g t c a g g g g c g t g a a t t t t c c c a g c a a g g a c c t t c t a c c g a c g g a c c a c a t g g t a c g g g t c c a g t c c c c g c a c t t a a a a g g g t c g t t

KasINarISfoIPluTI 480 130 135 140 145 GAVMQKKTKGWEPNTEMMYP mRuby RRRDAEENQGLGAQHRDDVP GAVMQKKTKGWEPNTEMMYP AGHHLLFGLAPLGVGLHHVG c g g c g c c g t g a t g c a g a a g a a a a c c a a g g g c t g g g a g c c c a a c a c c g a g a t g a t g t a c c c g c c g c g g c a c t a c g t c t t c t t t t g g t t c c c g a c c c t c g g g t t g t g g c t c t a c t a c a t g g g

540 150 155 160 165 ADGGLRGYTHMALKVDGGGH mRuby R* ADGGLRGYTHMALKVDGGGH SVASQSAVGVHGQLHVASPV c g c t g a c g g c g g a c t g a g a g g c t a c a c c c a c a t g g c c c t g a a g g t g g a c g g c g g a g g g c a g c g a c t g c c g c c t g a c t c t c c g a t g t g g g t g t a c c g g g a c t t c c a c c t g c c g c c t c c c g t

Bpu10I 600 170 175 180 185 LSCSFVTTYRSKKTVGNIKM mRuby LSCSFVTTYRSKKTVGNIKM QAAAEHGGVSGLFGHAVDLH c c t g a g c t g c a g c t t c g t g a c c a c c t a c c g a t c c a a g a a a a c c g t g g g c a a c a t c a a g a t g g a c t c g a c g t c g a a g c a c t g g t g g a t g g c t a g g t t c t t t t g g c a c c c g t t g t a g t t c t a

EarI 660 190 195 200 205 PGIHAVDHRLERLEESDNEM mRuby PGIHAVDHRLERLEESDNEM GADVGHVVPQFPQFLGVVLH g c c c g g c a t c c a c g c c g t g g a c c a c c g g c t g g a a a g g c t g g a a g a g t c c g a c a a c g a g a t c g g g c c g t a g g t g c g g c a c c t g g t g g c c g a c c t t t c c g a c c t t c t c a g g c t g t t g c t c t a

BspEIBpuEIBsgI 720 210 215 220 225 FVVQREHAVAKFAGLGGG SG mRuby FVVQREHAVAKFAGLGGGSG EHHLPLVGHGLEGAQASPGS g t t c g t g g t g c a g c g g g a g c a c g c c g t g g c c a a g t t c g c c g g c c t g g g c g g a g g g t c c g g c a a g c a c c a c g t c g c c c t c g t g c g g c a c c g g t t c a a g c g g c c g g a c c c g c c t c c c a g g c c

SalIAccIHincIIBglIIPaeR7IXhoIEco53kISacIHindIIIBstBIEcoRI 780 (in frame with mRuby) LRSRAQASNSAVDMADDFGF LRSRAQASNSAVDMADDFGF ESRSSLSRIRCDVHSIVKAE a c t c a g a t c t c g a g c t c a a g c t t c g a a t t c t g c a g t c g a c a t g g c t g a t g a c t t t g g c t t t g a g t c t a g a g c t c g a g t t c g a a g c t t a a g a c g t c a g c t g t a c c g a c t a c t g a a a c c g a a

EagINotI 840 (in frame with mRuby) FSSSESGAPEAAEEDPAAAF FSSSESGAPEAAEEDPAAAF ERRRLTTGRLRRLLVRRGGE c t t c t c g t c g t c g g a g a g t g g t g c c c c g g a g g c g g c g g a g g a g g a c c c g g c g g c c g c c t t g a a g a g c a g c a g c c t c t c a c c a c g g g g c c t c c g c c g c c t c c t c c t g g g c c g c c g g c g g a a

900 (in frame with mRuby) LAQQESEIAGIENDEGFGAP LAQQESEIAGIENDEGFGAP QGLLLALNCAYLVVLAEPCR c c t g g c c c a g c a g g a g a g c g a g a t t g c a g g c a t a g a g a a c g a c g a g g g c t t c g g g g c a c c g g a c c g g g t c g t c c t c t c g c t c t a a c g t c c g t a t c t c t t g c t g c t c c c g a a g c c c c g t g g

PspOMIApaIBssSαIBssSIBspMIPaqCIBfuAI 960 (in frame with mRuby) AGSHAAPAQPGPTSGAGSED AGSHAAPAQPGPTSGAGSED GAAM t g c c g g c a g c c a t g c g g c c c c c g c a c a g c c g g g c c c c a c g a g t g g g g c t g g t t c t g a g g a a c g g c c g t c g g t a c g c c g g g g g c g t g t c g g c c c g g g g t g c t c a c c c c g a c c a a g a c t c c t

TsoIPshAI 1020 (in frame with mRuby) MGTTVNGDVFQEANGPADGY MGTTVNGDVFQEANGPADGY c a t g g g g a c c a c a g t c a a t g g a g a t g t g t t t c a g g a g g c c a a c g g t c c t g c t g a t g g c t a g t a c c c c t g g t g t c a g t t a c c t c t a c a c a a a g t c c t c c g g t t g c c a g g a c g a c t a c c g a t

BsrDI 1080 (in frame with mRuby) AAIAQADRLTQEPESIRKWR AAIAQADRLTQEPESIRKWR c g c a g c c a t t g c c c a g g c t g a c a g g c t g a c c c a g g a g c c t g a g a g c a t c c g c a a g t g g c g g c g t c g g t a a c g g g t c c g a c t g t c c g a c t g g g t c c t c g g a c t c t c g t a g g c g t t c a c c g c

1140 (in frame with mRuby) EEQRKRLQELDAASKVTEQE EEQRKRLQELDAASKVTEQE a g a g g a g c a g a g g a a a c g g c t g c a a g a g c t g g a t g c t g c a t c t a a g g t c a c g g a a c a g g a t c t c c t c g t c t c c t t t g c c g a c g t t c t c g a c c t a c g a c g t a g a t t c c a g t g c c t t g t c c t

BpmI 1200 (in frame with mRuby) WREKAKKDLEEWNQRQSEQV WREKAKKDLEEWNQRQSEQV a t g g c g g g a g a a g g c c a a g a a g g a c c t g g a g g a g t g g a a c c a g c g c c a g a g t g a a c a a g t t a c c g c c c t c t t c c g g t t c t t c c t g g a c c t c c t c a c c t t g g t c g c g g t c t c a c t t g t t c a

BsaI 1260 (in frame with mRuby) EKNKINNRASEEAFVKESKE EKNKINNRASEEAFVKESKE a g a g a a g a a c a a g a t c a a c a a c c g g g c a t c c g a g g a g g c t t t c g t g a a g g a a t c c a a g g a t c t c t t c t t g t t c t a g t t g t t g g c c c g t a g g c t c c t c c g a a a g c a c t t c c t t a g g t t c c t

1320 (in frame with mRuby) ETPGTEWEKVAQLCDFNPKS ETPGTEWEKVAQLCDFNPKS g g a g a c c c c a g g c a c a g a g t g g g a g a a g g t g g c c c a g c t a t g t g a c t t c a a c c c c a a g a g c c t c t g g g g t c c g t g t c t c a c c c t c t t c c a c c g g g t c g a t a c a c t g a a g t t g g g g t t c t c

BtsαIBtsI 1380 (in frame with mRuby) SKQCKDVSRLRSVLMSLKQT SKQCKDVSRLRSVLMSLKQT c a g c a a g c a g t g c a a a g a t g t g t c c c g c c t g c g c t c g g t g c t c a t g t c c c t g a a g c a g a c g t c g t t c g t c a c g t t t c t a c a c a g g g c g g a c g c g a g c c a c g a g t a c a g g g a c t t c g t c t g

BsaBI*XbaI*BamHIDrdI 1440 (in frame with mRuby) PLSR* PLSR* g c c a c t g t c c c g c t a g g g a t c c a c c g g a t c t a g a t a a c t g a t c a t a a t c a g c c a t a c c a c c g g t g a c a g g g c g a t c c c t a g g t g g c c t a g a t c t a t t g a c t a g t a t t a g t c g g t a t g g t g

End (1443) 1443 a t t t a a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

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Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.