Addgene pBABEpuro-HA-MEK5 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pBABEpuro-HA-MEK5 Sequencing Result

Map View

1000 750 500 250 End (1091) BpmI (1086) Bpu10I (1071) XmnI (1053) PluTI - HaeII (1021) SfoI (1019) NarI - BsaHI (1018) KasI - BanI (1017) MmeI (978) PvuII - MspA1I (969) BmgBI (897) Esp3I - BsmBI (887) DraI (878) StuI (857) NspI (823) PciI - AflIII (819) TatI - BsrGI (766) PsiI (698) SspI (620) AccI (587) EcoRV (550) MscI (524) BbsI (482) PspFI (451) BseYI (447) PstI (356) BstXI (315) BsrDI (293) BsaBI * (248) EarI (172) BaeGI - BsiHKAI - Bsp1286I - Bme1580I (149) ApaLI (145) CsiI - SexAI * (89) BtgI - NcoI (80) BmtI (61) NheI (57) BamHI (7) BsiEI (5) EagI (2) Start (0) HA pBABEpuro-HA-MEK5 1091 bp

Sequence View

NheIStart (0) EagIBsiEIBamHI 5ʹ 3ʹ 60 1 5 YPYDVPDYA LWL HA PAGSAATMYPYDVPDYALWL c c g g c c g g a t c c g c c g c c a c c a t g t a c c c a t a c g a t g t t c c a g a t t a c g c t c t g t g g c t a g g c c g g c c t a g g c g g c g g t g g t a c a t g g g t a t g c t a c a a g g t c t a a t g c g a g a c a c c g a t

SexAI*CsiINcoIBtgIBmtI 120 (in frame with HA) ALGPFPAMENQVLVIRIKIP ALGPFPAMENQVLVIRIKIP g c c c t t g g c c c c t t t c c t g c c a t g g a g a a c c a g g t g c t g g t a a t t c g c a t c a a g a t c c c a c g g g a a c c g g g g a a a g g a c g g t a c c t c t t g g t c c a c g a c c a t t a a g c g t a g t t c t a g g g t

EarIApaLIBme1580IBsp1286IBsiHKAIBaeGI 180 (in frame with HA) NSGAVDWTVHSGPQLLFRDV NSGAVDWTVHSGPQLLFRDV a a t a g t g g c g c g g t g g a c t g g a c a g t g c a c t c c g g g c c g c a g t t a c t c t t c a g g g a t g t g t t a t c a c c g c g c c a c c t g a c c t g t c a c g t g a g g c c c g g c g t c a a t g a g a a g t c c c t a c a c

240 (in frame with HA) LDVIGQVLPEATTTAFEYED LDVIGQVLPEATTTAFEYED c t g g a t g t g a t a g g c c a g g t t c t g c c t g a a g c a a c a a c t a c a g c a t t t g a a t a t g a a g a t g a c c t a c a c t a t c c g g t c c a a g a c g g a c t t c g t t g t t g a t g t c g t a a a c t t a t a c t t c t a

BsrDIBsaBI* 300 (in frame with HA) EDGDRITVRSDEEMKAMLSY EDGDRITVRSDEEMKAMLSY g a a g a t g g t g a t c g a a t t a c a g t g a g a a g t g a t g a g g a a a t g a a g g c a a t g c t g t c a t a t c t t c t a c c a c t a g c t t a a t g t c a c t c t t c a c t a c t c c t t t a c t t c c g t t a c g a c a g t a t a

PstIBstXI 360 (in frame with HA) YYSTVMEQQVNGQLIEPLQI YYSTVMEQQVNGQLIEPLQI t a t t a t t c c a c a g t a a t g g a a c a g c a a g t a a a t g g a c a g t t a a t a g a g c c t c t g c a g a t a a t a a t a a g g t g t c a t t a c c t t g t c g t t c a t t t a c c t g t c a a t t a t c t c g g a g a c g t c t a t

420 (in frame with HA) FPRACKPPGERNIHGLKVNT FPRACKPPGERNIHGLKVNT t t t c c a a g a g c c t g c a a g c c t c c t g g g g a a c g g a a c a t a c a t g g c c t g a a g g t g a a t a c t a a a g g t t c t c g g a c g t t c g g a g g a c c c c t t g c c t t g t a t g t a c c g g a c t t c c a c t t a t g a

BseYIPspFI 480 (in frame with HA) RAGPSQHSSPAVSDSLPSNS RAGPSQHSSPAVSDSLPSNS c g g g c c g g a c c c t c t c a a c a c a g c a g c c c a g c a g t c t c a g a t t c a c t t c c a a g c a a t a g c g c c c g g c c t g g g a g a g t t g t g t c g t c g g g t c g t c a g a g t c t a a g t g a a g g t t c g t t a t c g

MscIBbsI 540 (in frame with HA) LKKSSAELKKILANGQMNEQ LKKSSAELKKILANGQMNEQ t t a a a g a a g t c t t c t g c t g a a c t g a a a a a a a t a c t a g c c a a t g g c c a g a t g a a t g a a c a a a a t t t c t t c a g a a g a c g a c t t g a c t t t t t t t a t g a t c g g t t a c c g g t c t a c t t a c t t g t t

AccIEcoRV 600 (in frame with HA) DIRYRDTLGHGNGGTVYKAY DIRYRDTLGHGNGGTVYKAY g a c a t a c g a t a t c g g g a c a c t c t t g g t c a t g g c a a c g g a g g c a c a g t c t a c a a a g c a t a t c t g t a t g c t a t a g c c c t g t g a g a a c c a g t a c c g t t g c c t c c g t g t c a g a t g t t t c g t a t a

SspI 660 (in frame with HA) HVPSGKILAVKVILLDITLE HVPSGKILAVKVILLDITLE c a t g t c c c g a g t g g g a a a a t a t t a g c t g t a a a g g t c a t a c t a c t a g a t a t t a c a c t g g a a g t a c a g g g c t c a c c c t t t t a t a a t c g a c a t t t c c a g t a t g a t g a t c t a t a a t g t g a c c t t

PsiI 720 (in frame with HA) LQKQIMSELEILYKCDSSYI LQKQIMSELEILYKCDSSYI c t t c a g a a g c a a a t t a t g t c t g a a t t g g a a a t t c t t t a t a a g t g c g a t t c a t c a t a t a t c g a a g t c t t c g t t t a a t a c a g a c t t a a c c t t t a a g a a a t a t t c a c g c t a a g t a g t a t a t a g

BsrGITatI 780 (in frame with HA) IGFYGAFFVENRISICTEFM IGFYGAFFVENRISICTEFM a t t g g a t t t t a t g g a g c a t t t t t t g t a g a a a a c a g g a t t t c a a t a t g t a c a g a a t t c a t g t a a c c t a a a a t a c c t c g t a a a a a a c a t c t t t t g t c c t a a a g t t a t a c a t g t c t t a a g t a c

AflIIIPciINspI 840 (in frame with HA) DGGSLDVYRKMPEHVLGRIA DGGSLDVYRKMPEHVLGRIA g a t g g g g g a t c t t t g g a t g t a t a t a g g a a a a t g c c a g a a c a t g t c c t t g g a a g a a t t g c a c t a c c c c c t a g a a a c c t a c a t a t a t c c t t t t a c g g t c t t g t a c a g g a a c c t t c t t a a c g t

BmgBIBsmBIEsp3IDraIStuI 900 (in frame with HA) VAVVKGLTYLWSLKILHRDV VAVVKGLTYLWSLKILHRDV g t a g c a g t t g t t a a a g g c c t t a c t t a t t t g t g g a g t t t a a a g a t t t t a c a t a g a g a c g t g c a t c g t c a a c a a t t t c c g g a a t g a a t a a a c a c c t c a a a t t t c t a a a a t g t a t c t c t g c a c

960 (in frame with HA) KPSNMLVNTRGQVKLCDFGV KPSNMLVNTRGQVKLCDFGV a a g c c c t c c a a t a t g c t a g t a a a c a c a a g a g g a c a g g t t a a g c t g t g t g a t t t t g g a g t t t t c g g g a g g t t a t a c g a t c a t t t g t g t t c t c c t g t c c a a t t c g a c a c a c t a a a a c c t c a a

BanIKasIBsaHINarISfoIMmeIMspA1IPvuII 1020 (in frame with HA) STQLVNSIAKTYVGTNAYMA STQLVNSIAKTYVGTNAYMA a g c a c t c a g c t g g t g a a t t c t a t a g c c a a g a c g t a t g t t g g a a c a a a t g c t t a t a t g g c g t c g t g a g t c g a c c a c t t a a g a t a t c g g t t c t g c a t a c a a c c t t g t t t a c g a a t a t a c c g c

Bpu10IXmnIHaeIIPluTI 1080 (in frame with HA) PERISGEQYGIHSDVWSLGI PERISGEQYGIHSDVWSLGI c c t g a a a g g a t t t c a g g g g a g c a g t a t g g a a t t c a t t c t g a t g t c t g g a g c t t a g g a a t c g g a c t t t c c t a a a g t c c c c t c g t c a t a c c t t a a g t a a g a c t a c a g a c c t c g a a t c c t t a g

End (1091) BpmI 3ʹ 5ʹ 1091 SFM SFM t c t t t t a t g g a a g a a a a t a c c t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.