Addgene pTXG-hTDG-K330A Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pTXG-hTDG-K330A Sequencing Result

Map View

800 600 400 200 End (879) NdeI (860) EarI (802) BseRI (763) BmrI (737) PflFI - Tth111I (729) EcoRI (650) SmlI - XhoI - PaeR7I (644) BsaBI (639) SpeI (618) EcoNI (608) BtgZI (600) DrdI (536) BsiHKAI (507) TatI - BsrGI (463) BsiWI (452) BaeGI Bme1580I (430) AatII (396) ZraI (394) BfuAI BspMI (302) SacII (285) MspA1I (284) BtgI (282) BsaI (208) TaqII (158) NaeI (124) MreI - SgrAI - NgoMIV (122) MluI - AflIII (76) XmnI (63) BstXI (49) AgeI (32) StuI (20) MflI * - BstYI - BamHI (11) BspEI * (8) Start (0) Mxe GyrA intein pTXG-hTDG-K330A 879 bp

Sequence View

BstXIAgeIStuIStart (0) BspEI*BamHIBstYIMflI* 5ʹ 3ʹ 60 (in frame with Mxe GyrA intein) SMGSGLGSNLGTLGT 195 AHSVF Mxe GyrA intein SMGSGLGSNLGTLGTAHSVF g g g a c a t t c c g g a t c c g a g g c c t g a g t t c a g a c c g g t g a g g c c a g t a g c g t g g c t g a c g a c c c t g t a a g g c c t a g g c t c c g g a c t c a a g t c t g g c c a c t c c g g t c a t c g c a c c g a c t g c t

AflIIIMluIXmnI 120 190 185 180 175 GNTIFAHDATDVRLSYVPQV Mxe GyrA intein GNTIFAHDATDVRLSYVPQV a c c c g t t c g t g a t a a a c g c g t g g t c t g c c g t g t c g a c a c g a a g g c t a t a c a c c g g c t g c a t g g g c a a g c a c t a t t t g c g c a c c a g a c g g c a c a g c t g t g c t t c c g a t a t g t g g c c g a c g t

TaqIINgoMIVSgrAIMreINaeI 180 170 165 160 155 GADTVSAVKAYYFRGDTLED Mxe GyrA intein GADTVSAVKAYYFRGDTLED c g c c g g c g t c g g t g a c a c t g g c g a c t t t c g c g t a g t a g a a c c g c c c g t c g g t c a g c t c g t g c g g c c g c a g c c a c t g t g a c c g c t g a a a g c g c a t c a t c t t g g c g g g c a g c c a g t c g a g c a

BsaI 240 150 145 140 135 AIAQADPDRHHAELFRVLGP Mxe GyrA intein AIAQADPDRHHAELFRVLGP c g g c g a t a g c t t g g g c g t c c g g g t c t c g g t g g t g t g c t t c c a a g a a a c g c a c c a g t c c a g g c c g c t a t c g a a c c c g c a g g c c c a g a g c c a c c a c a c g a a g g t t c t t t g c g t g g t c a g g t c

BtgIMspA1ISacII 300 130 125 120 115 VGVTYTTPAFEPKGRAFGAC Mxe GyrA intein VGVTYTTPAFEPKGRAFGAC g g a c g c c g a c t g t g t a g g t t g t g g g c g c a a a t t c g g g t t t c c c g c g g g c a a a a c c t g c a c c c t g c g g c t g a c a c a t c c a a c a c c c g c g t t t a a g c c c a a a g g g c g c c c g t t t t g g a c g t g

BspMIBfuAI 360 110 105 100 95 DVSFASRQIVAYDGPKIEDI Mxe GyrA intein DVSFASRQIVAYDGPKIEDI a g t c g a c g c t g a a t g c g c t g c g t t g a a t c a c c g c g t a a t c g c c c g g c t t g a t t t c g t c g a t c a g c t g c g a c t t a c g c g a c g c a a c t t a g t g g c g c a t t a g c g g g c c g a a c t a a a g c a g c t

ZraIAatII 420 90 85 80 75 LKWLLTPVGAVDVLCLLPHN Mxe GyrA intein LKWLLTPVGAVDVLCLLPHN t c a g c t t c c a c a g c a g g g t c g g c a c c c c g g c g a c g t c g a c c a a a c a c a a c a a c g g g t g g t a g t c g a a g g t g t c g t c c c a g c c g t g g g g c c g c t g c a g c t g g t t t g t g t t g t t g c c c a c c a

BsrGITatIBsiWIBme1580IBaeGI 480 70 65 60 55 ATGTVRLGEVTRVTYVPHEG Mxe GyrA intein ATGTVRLGEVTRVTYVPHEG t c g c g g t g c c c g t c a c a c g c a g a c c t t c g a c c g t a c g c a c c g t g t a c a c c g g a t g c t c g c a g c g c c a c g g g c a g t g t g c g t c t g g a a g c t g g c a t g c g t g g c a c a t g t g g c c t a c g a g c g

DrdIBsiHKAI 540 50 45 40 35 SHFLRDALVPNGHRDLVKLD Mxe GyrA intein SHFLRDALVPNGHRDLVKLD c g g a g t g g a a c a g c c g g t c g g c g a g c a c g g g a t t g c c a t g c c g g t c a a g g a c t t t c a g g t g c c t c a c c t t g t c g g c c a g c c g c t c g t g c c c t a a c g g t a c g g c c a g t t c c t g a a a g t c c a

600 30 25 20 15 IANDSNPRAGPVIDAIRVSE Mxe GyrA intein IANDSNPRAGPVIDAIRVSE c g a t g g c g t t g t c a c t g t t g g g c c g c g c a c c c g g c a c g a t g t c g g c g a t g c g t a c c g a c t g c t a c c g c a a c a g t g a c a a c c c g g c g c g t g g g c c g t g c t a c a g c c g c t a c g c a t g g c t g a

EcoRIPaeR7IXhoISmlIBsaBISpeIEcoNIBtgZI 660 10 5 1 GEPLAVLADGCIC Mxe GyrA intein GEPLAVLADGCICMELFEAH c g c c c t c g g g t a g g g c a a c t a g t g c a t c t c c g c a g a t g c a c a t c t c g a g g a a t t c a g c a t g c g g g a g c c c a t c c c g t t g a t c a c g t a g a g g c g t c t a c g t g t a g a g c t c c t t a a g t c g t a

720 SEEEQEQTGCHNSFSPIQDT g g c t t t c t t c t t c c t g t t c t t g t g t t c c a c a g t g a t t a c t a a a g g a a g g a a t t t g g t c t g c c g a a a g a a g a a g g a c a a g a a c a c a a g g t g t c a c t a a t g a t t t c c t t c c t t a a a c c a g a c

BseRIBmrITth111IPflFI 780 FSQTMWQGNPIGSFASEGRL t a a a t g a c t g g g t c a t c c a c t g c c c a t t a g g a a t g c c a c t g a a a g c t g a t t c t c c t c t t a a t t t a c t g a c c c a g t a g g t g a c g g g t a a t c c t t a c g g t g a c t t t c g a c t a a g a g g a g a a t

EarI 840 EVSEILGNSSFGCPESSCPN a c t c c a c g c t c t c a a t t a g c c c a t t t g a a g a g a a g c c a c a a g g t t c a c t g c t g c a t g g a t t g a g g t g c g a g a g t t a a t c g g g t a a a c t t c t c t t c g g t g t t c c a a g t g a c g a c g t a c c t a

End (879) NdeI 3ʹ 5ʹ 879 EGYAGGYAAEYG t t t c t c c g t a a g c a c c a c c a t a t g c t g c c t c a t a a c c t g a a a g a g g c a t t c g t g g t g g t a t a c g a c g g a g t a t t g g a c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.