Addgene Dronpa-Lifeact-7 - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: Dronpa-Lifeact-7


Map View

800 600 400 200 End (994) MfeI (961) DraI (913) BsaBI * (873) XbaI * (865) NotI - EagI (855) NmeAIII (854) BsiHKAI - Bsp1286I (807) DraIII (742) PsiI (702) MscI (672) AcuI - Eco57MI (650) EarI (566) ApoI (522) BbsI (465) Bsu36I (423) AccI (399) EciI (327) DrdI (299) XmnI (269) NruI (242) AleI (170) BseRI (158) AgeI - BsrFI - BsaWI (157) BamHI (151) BclI * (114) BstXI (103) XcmI (100) BmtI (90) NheI (86) CMV-F (14 .. 34) Start (0) CMVd2 promoter Lifeact Dronpa-Green1 Kozak sequence Kozak sequence Dronpa-Lifeact-7 994 bp

Sequence View

Start (0) 5ʹ 3ʹ 60 CMVd2 promoter C G C A A A T G G G C G G T A G G C G T G CMV-F RGMSAFPRYAHVTPPRYLCL t c c g c c c c a t t g a c g c a a a t g g g c g g t a g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g a a g g c g g g g t a a c t g c g t t t a c c c g c c a t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c t

BclI*XcmIBstXIBmtINheI 120 1 5 MGVADLIK CMVd2 promoter Lifeact Kozak sequence MGVADLIK QNLSGDSGSAGGHAHGVQDL g c t g g t t t a g t g a a c c g t c a g a t c c g c t a g c g c c a c c a t g g g c g t g g c c g a c t t g a t c a a c g a c c a a a t c a c t t g g c a g t c t a g g c g a t c g c g g t g g t a c c c g c a c c g g c t g a a c t a g t t

AleIBamHIBsaWIBsrFIAgeIBseRI 180 10 15 KFESISKEE (in frame with Lifeact) GDPPVAT 1 MVSV Lifeact Dronpa-Green1 Kozak sequence KFESISKEEGDPPVATMVSV LELGDGLLLPIWRDGGHHTH g a a g t t c g a g t c c a t c t c c a a g g a g g a g g g g g a t c c a c c g g t c g c c a c c a t g g t g a g t g t c t t c a a g c t c a g g t a g a g g t t c c t c c t c c c c c t a g g t g g c c a g c g g t g g t a c c a c t c a c a

240 5 10 15 20 IKPDMKIKLRMEGAVNGHPF Dronpa-Green1 IKPDMKIKLRMEGAVNGHPF NFWVHLDLQTHFASYISVRE g a t t a a a c c a g a c a t g a a g a t c a a g c t g c g t a t g g a a g g c g c t g t a a a t g g a c a c c c g t t c t a a t t t g g t c t g t a c t t c t a g t t c g a c g c a t a c c t t c c g c g a c a t t t a c c t g t g g g c a a

DrdIXmnINruI 300 25 30 35 40 AIEGVGLGKPFEGKQSMDLK Dronpa-Green1 AIEGVGLGKPFEGKQSMDLK RNFSNAKPLRELSFLTHVKF c g c g a t t g a a g g a g t t g g c c t t g g g a a g c c t t t c g a g g g a a a a c a g a g t a t g g a c c t t a a g c g c t a a c t t c c t c a a c c g g a a c c c t t c g g a a a g c t c c c t t t t g t c t c a t a c c t g g a a t t

EciI 360 45 50 55 60 VKEGGPLPFAYDILTTVFCY Dronpa-Green1 VKEGGPLPFAYDILTTVFCY DFFASRQREGIVDQCSHETV a g t c a a a g a a g g c g g a c c t c t g c c t t t c g c c t a t g a c a t c t t g a c a a c t g t g t t c t g t t a t c a g t t t c t t c c g c c t g g a g a c g g a a a g c g g a t a c t g t a g a a c t g t t g a c a c a a g a c a a t

AccI 420 65 70 75 80 GNRVFAKYPENIVDYFKQSF Dronpa-Green1 GNRVFAKYPENIVDYFKQSF AVPYEGFVWFIYYVIELLRK c g g c a a c a g g g t a t t c g c c a a a t a c c c a g a a a a t a t a g t a g a c t a t t t c a a g c a g t c g t t g c c g t t g t c c c a t a a g c g g t t t a t g g g t c t t t t a t a t c a t c t g a t a a a g t t c g t c a g c a a

BbsIBsu36I 480 85 90 95 100 PEGYSWERSMNYEDGGICNA Dronpa-Green1 PEGYSWERSMNYEDGGICNA RLAVRPFSAHIVFVPANTVR t c c t g a g g g c t a c t c t t g g g a a c g a a g c a t g a a t t a c g a a g a c g g g g g c a t t t g t a a c g c a g g a c t c c c g a t g a g a a c c c t t g c t t c g t a c t t a a t g c t t c t g c c c c c g t a a a c a t t g c g

ApoI 540 105 110 115 120 TNDITLDGDCYIYEIRFDGV Dronpa-Green1 TNDITLDGDCYIYEIRFDGV CVVYGQITVTIDIFNSKITH g a c a a a c g a c a t a a c c c t g g a t g g t g a c t g t t a t a t c t a t g a a a t t c g a t t t g a t g g t g t c t g t t t g c t g t a t t g g g a c c t a c c a c t g a c a a t a t a g a t a c t t t a a g c t a a a c t a c c a c a

EarI 600 125 130 135 140 NFPANGPVMQKRTVKWEPST Dronpa-Green1 NFPANGPVMQKRTVKWEPST VKRGITWNHLLPSHFPLWGS g a a c t t t c c t g c c a a t g g t c c a g t t a t g c a g a a g a g g a c t g t g a a a t g g g a g c c a t c c a c c t t g a a a g g a c g g t t a c c a g g t c a a t a c g t c t t c t c c t g a c a c t t t a c c c t c g g t a g g t g

Eco57MIAcuI 660 145 150 155 160 EKLYVRDGVLKGDVNMALSL Dronpa-Green1 EKLYVRDGVLKGDVNMALSL LFQIHTISHQLTINVHSQRK t g a g a a a t t g t a t g t g c g t g a t g g a g t g c t g a a g g g t g a t g t t a a c a t g g c t c t g t c g c t a c t c t t t a a c a t a c a c g c a c t a c c t c a c g a c t t c c c a c t a c a a t t g t a c c g a g a c a g c g a

PsiIMscI 720 165 170 175 180 EGGGHYRCDFKTTYKAKKVV Dronpa-Green1 EGGGHYRCDFKTTYKAKKVV FSTAMVSTVEFSSIFSLLND t g a a g g a g g t g g c c a t t a c c g a t g t g a c t t c a a a a c t a c t t a t a a a g c t a a g a a g g t t g t a c t t c c t c c a c c g g t a a t g g c t a c a c t g a a g t t t t g a t g a a t a t t t c g a t t c t t c c a a c a

DraIII 780 185 190 195 200 QLPDYHFVDHHIEIKSHDKD Dronpa-Green1 QLPDYHFVDHHIEIKSHDKD LQWVIVKHVVVNLNFAVVFI c c a g t t g c c a g a c t a t c a c t t t g t g g a c c a c c a c a t t g a g a t t a a a a g c c a c g a c a a a g a g g t c a a c g g t c t g a t a g t g a a a c a c c t g g t g g t g t a a c t c t a a t t t t c g g t g c t g t t t c t

Bsp1286IBsiHKAI 840 205 210 215 220 YSNVNLHEHAEAHSELPRQA Dronpa-Green1 YSNVNLHEHAEAHSELPRQA VTINIQMLVGFRM t t a c a g t a a t g t t a a t c t g c a t g a g c a c g c c g a a g c g c a t t c t g a g c t g c c g a g g c a g g c a a t g t c a t t a c a a t t a g a c g t a c t c g t g c g g c t t c g c g t a a g a c t c g a c g g c t c c g t c c g

BsaBI*XbaI*NmeAIIIEagINotI 900 225 K* Dronpa-Green1 K* c a a g t a a t g a t a g g c g g c c g c g a c t c t a g a t c a t a a t c a g c c a t a c c a c a t t t g t a g a g g g t t c a t t a c t a t c c g c c g g c g c t g a g a t c t a g t a t t a g t c g g t a t g g t g t a a a c a t c t c c

DraI 960 t t t t a c t t g c t t t a a a a a a c c t c c c a c a c c t c c c c c t g a a c c t g a a a c a t a a a a t g a a t g a a a a t g a a c g a a a t t t t t t g g a g g g t g t g g a g g g g g a c t t g g a c t t t g t a t t t t a c t t a c

End (994) MfeI 3ʹ 5ʹ 994 c a a t t g t t g t t g t t a a c t t g t t t a t t g c a g c t t a g t t a a c a a c a a c a a t t g a a c a a a t a a c g t c g a a t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.