Addgene pMyc-CMV1 - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pMyc-CMV1


Map View

PsiI (4590) F1ori-F (4529 .. 4550) DraIII (4465) NaeI (4359) NgoMIV (4357) F1ori-R (4319 .. 4338) Amp-R (3863 .. 3882) ScaI (3793) pBR322ori-F (2561 .. 2580) AflIII - PciI (2420) L4440 (2310 .. 2327) PasI (118) M13/pUC Forward (128 .. 150) M13 Forward (142 .. 159) BsrGI (270) SpeI (326) NdeI (560) SnaBI (666) CMV-F (845 .. 865) Eco53kI (892) SacI (894) PstI (963) Myc XbaI (1014) BamHI (1020) TspMI - XmaI (1024) SmaI (1026) AleI (1084) PspOMI (1188) ApaI (1192) BbsI (1208) BbvCI - Bpu10I (1312) BlpI (1356) BsmBI - Esp3I (1376) BstXI (1463) BfuAI - BspMI (1568) AgeI (1574) PflMI (1577) pBABE 3' (1673 .. 1693) CsiI - SexAI * (1759) SV40 promoter SfiI (1946) StuI (1992) AvrII (1993) AbsI - PaeR7I - PspXI - XhoI (2015) T7 (2046 .. 2065) M13 Reverse (2079 .. 2095) lac operator M13/pUC Reverse (2092 .. 2114) CAP binding site BspQI - SapI (2304) pMyc-CMV1 4688 bp

Sequence View

5ʹ 3ʹ 60 c c c a t t c g c c a t t c a g g c t g c g c a a c t g t t g g g a a g g g c g a t c g g t g c g g g c c t c t t c g c g g g t a a g c g g t a a g t c c g a c g c g t t g a c a a c c c t t c c c g c t a g c c a c g c c c g g a g a a g c g

PasI 120 t a t t a c g c c a g c t g g c g a a a g g g g g a t g t g c t g c a a g g c g a t t a a g t t g g g t a a c g c c c a a t a a t g c g g t c g a c c g c t t t c c c c c t a c a c g a c g t t c c g c t a a t t c a a c c c a t t g c g g g t

180 M13 fwd C C C A G T C A C G A C G T T G T A A A A C G M13/pUC Forward T G T A A A A C G A C G G C C A G T M13 Forward g g g t t t t c c c a g t c a c g a c g t t g t a a a a c g a c g g c c a g t g c c a a g c t g a t c t a a t c a a t a c c c a a a a g g g t c a g t g c t g c a a c a t t t t g c t g c c g g t c a c g g t t c g a c t a g a t t a g t t a t

240 t t g g c c a t t a g c c a t a t t a t t c a t t g g t t a t a t a g c a t a a a t c a a t a t t g g c t a t t g g c c a a c c g g t a a t c g g t a t a a t a a g t a a c c a a t a t a t c g t a t t t a g t t a t a a c c g a t a a c c g g

BsrGI 300 a t t g c a t a c g t t g t a t c c a t a t c a t a a t a t g t a c a t t t a t a t t g g c t c a t g t c c a a c a t t t a a c g t a t g c a a c a t a g g t a t a g t a t t a t a c a t g t a a a t a t a a c c g a g t a c a g g t t g t a a

SpeI 360 CMV enhancer a c c g c c a t g t t g a c a t t g a t t a t t g a c t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t t g g c g g t a c a a c t g t a a c t a a t a a c t g a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a

420 CMV enhancer a g t t c a t a g c c c a t a t a t g g a g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g t c a a g t a t c g g g t a t a t a c c t c a a g g c g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c

480 CMV enhancer c g a c c g c c c a g c g a c c c c c g c c c g t t g a c g t c a a t a g t g a c g t a t g t t c c c a t a g t a a c g g c t g g c g g g t c g c t g g g g g c g g g c a a c t g c a g t t a t c a c t g c a t a c a a g g g t a t c a t t g c

540 CMV enhancer c c a a t a g g g a c t t t c c a t t g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g g t t a t c c c t g a a a g g t a a c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c

NdeI 600 CMV enhancer g c a g t a c a t c a a g t g t a t c a t a t g c c a a g t c c g c c c c c t a t t g a c g t c a a t g a c g g t a a a c g t c a t g t a g t t c a c a t a g t a t a c g g t t c a g g c g g g g g a t a a c t g c a g t t a c t g c c a t t t

660 CMV enhancer t g g c c c g c c t a g c a t t a t g c c c a g t a c a t g a c c t t a c g g g a g t t t c c t a c t t g g c a g t a c a c c g g g c g g a t c g t a a t a c g g g t c a t g t a c t g g a a t g c c c t c a a a g g a t g a a c c g t c a t g

SnaBI 720 CMV enhancer CMV promoter a t c t a c g t a t t a g t c a t c g c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a c c a a t g g g t a g a t g c a t a a t c a g t a g c g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t g g t t a c c c

780 CMV promoter c g t g g a t a g c g g t t t g a c t c a c g g g g a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g g c a c c t a t c g c c a a a c t g a g t g c c c c t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c

840 CMV promoter a g t t t g t t t t g g c a c c a a a a t c a a c g g g a c t t t c c a a a a t g t c g t a a t a a c c c c g c c c c g t c a a a c a a a a c c g t g g t t t t a g t t g c c c t g a a a g g t t t t a c a g c a t t a t t g g g g c g g g g c

Eco53kISacI 900 CMV promoter C G C A A A T G G G C G G T A G G C G T G CMV-F t t g a c g c a a a t g g g c g g t a g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g a g c t c g t t t a a a c t g c g t t t a c c c g c c a t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c t c g a g c a a a t

960 1 5 10 MSALLILALVGA preprotrypsin leader g t g a a c c g t c a g a a t t a a t t c a c c a t g t c t g c a c t t c t g a t c c t a g c t c t t g t t g g a g c t c a c t t g g c a g t c t t a a t t a a g t g g t a c a g a c g t g a a g a c t a g g a t c g a g a a c a a c c t c g a

XbaIPstI 1020 15 AVA 1 5 10 EQKLISEEDL (in frame with Myc) LAASTLE preprotrypsin leader Myc g c a g t t g c t g a a c a a a a a c t c a t c t c a g a a g a g g a t c t g c t t g c g g c c t c g a c t c t a g a g c g t c a a c g a c t t g t t t t t g a g t a g a g t c t t c t c c t a g a c g a a c g c c g g a g c t g a g a t c t c

BamHITspMIXmaISmaI 1080 hGH poly(A) signal (in frame with Myc) DPGWHPCDPSPVPLLALEVA g a t c c c g g g t g g c a t c c c t g t g a c c c c t c c c c a g t g c c t c t c c t g g c c t t g g a a g t t g c c c t a g g g c c c a c c g t a g g g a c a c t g g g g a g g g g t c a c g g a g a g g a c c g g a a c c t t c a a c g g

AleI 1140 hGH poly(A) signal (in frame with Myc) TPVPTSLVLIKLSCIILSD* a c t c c a g t g c c c a c c a g c c t t g t c c t a a t a a a a t t a a g t t g c a t c a t t t t g t c t g a c t a g t g a g g t c a c g g g t g g t c g g a a c a g g a t t a t t t t a a t t c a a c g t a g t a a a a c a g a c t g a t c

PspOMIApaI 1200 hGH poly(A) signal g t g t c c t c t a t a a t a t t a t g g g g t g g a g g g g g g t g g t a t g g a g c a a g g g g c c c a a g t t g g c a c a g g a g a t a t t a t a a t a c c c c a c c t c c c c c c a c c a t a c c t c g t t c c c c g g g t t c a a c c

BbsI 1260 hGH poly(A) signal g a a g a c a a c c t g t a g g g c c t g c g g g g t c t a t t c g g g a a c c a a g c t g g a g t g c a g t g g c a c c t t c t g t t g g a c a t c c c g g a c g c c c c a g a t a a g c c c t t g g t t c g a c c t c a c g t c a c c g t g

Bpu10IBbvCI 1320 hGH poly(A) signal a a t c t t g g c t c a c t g c a a t c t c c g c c t c c t g g g t t c a a g c g a t t c t c c t g c c t c a g c c t c t t a g a a c c g a g t g a c g t t a g a g g c g g a g g a c c c a a g t t c g c t a a g a g g a c g g a g t c g g a g

BsmBIEsp3IBlpI 1380 hGH poly(A) signal c c g a g t t g t t g g g a t t c c a g g c a t g c a t g a c c a g g c t c a g c t a a t t t t t g t t t t t t t g g t g g c t c a a c a a c c c t a a g g t c c g t a c g t a c t g g t c c g a g t c g a t t a a a a a c a a a a a a a c c a

1440 hGH poly(A) signal a g a g a c g g g g t t t c a c c a t a t t g g c c a g g c t g g t c t c c a a c t c c t a a t c t c a g g t g a t c t t c t c t g c c c c a a a g t g g t a t a a c c g g t c c g a c c a g a g g t t g a g g a t t a g a g t c c a c t a g a

BstXI 1500 hGH poly(A) signal a c c c a c c t t g g c c t c c c a a a t t g c t g g g a t t a c a g g c g t g a a c c a c t g c t c c c t t c c c t g t g g g t g g a a c c g g a g g g t t t a a c g a c c c t a a t g t c c g c a c t t g g t g a c g a g g g a a g g g a c

1560 hGH poly(A) signal t c c t t c t g a t t t t a a a a t a a c t a t a c c a g c a g g a g g a c g t c c a g a c a c a g c a t a g g c t a c a g g a a g a c t a a a a t t t t a t t g a t a t g g t c g t c c t c c t g c a g g t c t g t g t c g t a t c c g a t g

BspMIBfuAIAgeIPflMI 1620 hGH poly(A) signal c t g c c a t g g c c c a a c c g g t g g g a c a t t t g a g t t g c t t g c t t g g c a c t g t c c t c t c a t g c g g a c g g t a c c g g g t t g g c c a c c c t g t a a a c t c a a c g a a c g a a c c g t g a c a g g a g a g t a c g c

1680 hGH poly(A) signal SV40 promoter C C T T A C A C pBABE 3' t t g g g t c c a c t c a g t a g a t g c c t g t t g a a t t g g g t a c g c g g c c a g c t t c t g t g g a a t g t g a a c c c a g g t g a g t c a t c t a c g g a c a a c t t a a c c c a t g c g c c g g t c g a a g a c a c c t t a c a c

1740 SV40 promoter A C A G T C A A T C C C A pBABE 3' t g t c a g t t a g g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g a c a g t c a a t c c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c

SexAI*CsiI 1800 SV40 promoter c a t c t c a a t t a g t c a g c a a c c a g g t g t g g a a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g g t a g a g t t a a t c a g t c g t t g g t c c a c a c c t t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c

1860 SV40 promoter SV40 ori t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c c a t a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g g t a

1920 SV40 promoter SV40 ori c c c g c c c c t a a c t c c g c c c a g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t g g g c g g g g a t t g a g g c g g g t c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a

SfiI 1980 SV40 promoter SV40 ori t a t t t a t g c a g a g g c c g a g g c c g c c t c g g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g a t a a a t a c g t c t c c g g c t c c g g c g g a g c c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c

PaeR7IPspXIXhoIAbsIStuIAvrII 2040 SV40 promoter SV40 ori c t t t t t t g g a g g c c t a g g c t t t t g c a a a a a g c t c c t c g a g g a a c t g a a a a a c c a g a a a g t g a a a a a a c c t c c g g a t c c g a a a a c g t t t t t c g a g g a g c t c c t t g a c t t t t t g g t c t t t c a

2100 T7 promoter M13 rev G G G A T A T C A C T C A G C A T A A T T7 C A G T A T C G A C A A A G G A C M13 Reverse G G A C A C A C T M13/pUC Reverse t a a t t c c c t a t a g t g a g t c g t a t t a a a t t c g t a a t c a t g t c a t a g c t g t t t c c t g t g t g a a t t a a g g g a t a t c a c t c a g c a t a a t t t a a g c a t t a g t a c a g t a t c g a c a a a g g a c a c a c t

2160 -10 -35 lac operator lac promoter T T A A C A A T A G G C G A M13/pUC Reverse a a t t g t t a t c c g c t c a c a a t t c c a c a c a a c a t a c g a g c c g g a a g c a t a a a g t g t a a a g c c t t a a c a a t a g g c g a g t g t t a a g g t g t g t t g t a t g c t c g g c c t t c g t a t t t c a c a t t t c g g

2220 CAP binding site t g g g g t g c c t a a t g a g t g a g c t a a c t c a c a t t a a t t g c g t t g c g c t c a c t g c c c g c t t t c a c c c c a c g g a t t a c t c a c t c g a t t g a g t g t a a t t a a c g c a a c g c g a g t g a c g g g c g a a a g

2280 c a g t c g g g a a a c c t g t c g t g c c a g c t g c a t t a a t g a a t c g g c c a a c g c g c g g g g a g a g g c g t c a g c c c t t t g g a c a g c a c g g t c g a c g t a a t t a c t t a g c c g g t t g c g c g c c c c t c t c c g

BspQISapI 2340 G A G C G A G T G A C T G A G C G A L4440 g g t t t g c g t a t t g g g c g c t c t t c c g c t t c c t c g c t c a c t g a c t c g c t g c g c t c g g t c g t t c c a a a c g c a t a a c c c g c g a g a a g g c g a a g g a g c g a g t g a c t g a g c g a c g c g a g c c a g c a a

2400 c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a a g g c g g t a a t a c g g t t a t c c a c a g a a t c a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t t c c g c c a t t a t g c c a a t a g g t g t c t t a g t

AflIIIPciI 2460 g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t

2520 ori a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a

2580 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g

2640 ori c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g

2700 ori g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a a t g c t c a c g c t g t a g g t a t c t c a g t c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t t a c g a g t g c g a c a t c c a t a g a g t c a

2760 ori t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g

2820 ori c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c

2880 ori c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t

2940 ori g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a c a c t a g a a g a a c a g t a t t t g g t a t c t g c c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g

3000 ori g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t

3060 ori a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t

3120 ori g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g

3180 t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t

3240 AmpR a a t t a a a a a t g a a g t t t t a a a t c a a t c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t t t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a

3300 285 280 275 270 *WHKILSAGIEAIQRNREDM AmpR *WHKILSAGIEAIQRNREDM t a c c a a t g c t t a a t c a g t g a g g c a c c t a t c t c a g c g a t c t g t c t a t t t c g t t c a t c c a t a a t g g t t a c g a a t t a g t c a c t c c g t g g a t a g a g t c g c t a g a c a g a t a a a g c a a g t a g g t a t

3360 265 260 255 250 TAQSGTTYIVVIRSPKGDPG AmpR TAQSGTTYIVVIRSPKGDPG g t t g c c t g a c t c c c c g t c g t g t a g a t a a c t a c g a t a c g g g a g g g c t t a c c a t c t g g c c c c c a a c g g a c t g a g g g g c a g c a c a t c t a t t g a t g c t a t g c c c t c c c g a a t g g t a g a c c g g g g

3420 245 240 235 230 LAAIIGRSGREGAGSKDAIF AmpR LAAIIGRSGREGAGSKDAIF a g t g c t g c a a t g a t a c c g c g a g a c c c a c g c t c a c c g g c t c c a g a t t t a t c a g c a a t a a a c t c a c g a c g t t a c t a t g g c g c t c t g g g t g c g a g t g g c c g a g g t c t a a a t a g t c g t t a t t t g

3480 225 220 215 210 WGAPLASRLLPGAVKDAEMW AmpR WGAPLASRLLPGAVKDAEMW c a g c c a g c c g g a a g g g c c g a g c g c a g a a g t g g t c c t g c a a c t t t a t c c g c c t c c a t c c a g g t c g g t c g g c c t t c c c g g c t c g c g t c t t c a c c a g g a c g t t g a a a t a g g c g g a g g t a g g t c

3540 205 200 195 190 DILQQRSALTLLEGTLLKRL AmpR DILQQRSALTLLEGTLLKRL t c t a t t a a t t g t t g c c g g g a a g c t a g a g t a a g t a g t t c g c c a g t t a a t a g t t t g c g c a a c a g a t a a t t a a c a a c g g c c c t t c g a t c t c a t t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g

3600 185 180 175 170 TTAMAVPMTTDREDNPIAEN AmpR TTAMAVPMTTDREDNPIAEN g t t g t t g c c a t t g c t a c a g g c a t c g t g g t g t c a c g c t c g t c g t t t g g t a t g g c t t c a t t c c a a c a a c g g t a a c g a t g t c c g t a g c a c c a c a g t g c g a g c a g c a a a c c a t a c c g a a g t a a g

3660 165 160 155 150 LEPEWRDLRTVHDGMNHLFA AmpR LEPEWRDLRTVHDGMNHLFA a g c t c c g g t t c c c a a c g a t c a a g g c g a g t t a c a t g a t c c c c c a t g t t g t g c a a a a a a g c g t c g a g g c c a a g g g t t g c t a g t t c c g c t c a a t g t a c t a g g g g g t a c a a c a c g t t t t t t c g c

3720 145 140 135 130 TLEKPGGITTLLLNAATNDS AmpR TLEKPGGITTLLLNAATNDS g t t a g c t c c t t c g g t c c t c c g a t c g t t g t c a g a a g t a a g t t g g c c g c a g t g t t a t c a c t c c a a t c g a g g a a g c c a g g a g g c t a g c a a c a g t c t t c a t t c a a c c g g c g t c a c a a t a g t g a g

3780 125 120 115 110 MTIAASCLERVTMGDTLHKE AmpR MTIAASCLERVTMGDTLHKE a t g g t t a t g g c a g c a c t g c a t a a t t c t c t t a c t g t c a t g c c a t c c g t a a g a t g c t t t t c t t a c c a a t a c c g t c g t g a c g t a t t a a g a g a a t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a

ScaI 3840 105 100 95 90 TVPSYEVLDNQSYHIRRGLQ AmpR TVPSYEVLDNQSYHIRRGLQ g t g a c t g g t g a g t a c t c a a c c a a g t c a t t c t g a g a a t a g t g t a t g c g g c g a c c g a g t t g c c a c t g a c c a c t c a t g a g t t g g t t c a g t a a g a c t c t t a t c a c a t a c g c c g c t g g c t c a a c g

3900 85 80 75 70 EQGADIRSLVAGCLLVKFTS AmpR A T A A T A C C G C G C C A C A T A G C Amp-R EQGADIRSLVAGCLLVKFTS t c t t g c c c g g c g t c a a t a c g g g a t a a t a c c g c g c c a c a t a g c a g a a c t t t a a a a g t g c t c a g a a c g g g c c g c a g t t a t g c c c t a t t a t g g c g c g g t g t a t c g t c t t g a a a t t t t c a c g a g

3960 65 60 55 50 MMPFREEPRFSELIKGSNLD AmpR MMPFREEPRFSELIKGSNLD a t c a t t g g a a a a c g t t c t t c g g g g c g a a a a c t c t c a a g g a t c t t a c c g c t g t t g a g a t c c t a g t a a c c t t t t g c a a g a a g c c c c g c t t t t g a g a g t t c c t a g a a t g g c g a c a a c t c t a g g

4020 45 40 35 30 LEIYGVRAGLQDEADKVKVL AmpR LEIYGVRAGLQDEADKVKVL a g t t c g a t g t a a c c c a c t c g t g c a c c c a a c t g a t c t t c a g c a t c t t t t a c t t t c a c c a g c t c a a g c t a c a t t g g g t g a g c a c g t g g g t t g a c t a g a a g t c g t a g a a a a t g a a a g t g g t c g

4080 25 TEPH 20 15 10 signal sequence AFVPLCFAAFFPILAV AmpR TEPHAFVPLCFAAFFPILAV g t t t c t g g g t g a g c a a a a a c a g g a a g g c a a a a t g c c g c a a a a a a g g g a a t a a g g g c g a c a c a a a g a c c c a c t c g t t t t t g t c c t t c c g t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t

4140 5 1 signal sequence RFHQISM AmpR AmpR promoter RFHQISM c g g a a a t g t t g a a t a c t c a t a c t c t t c c t t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t g c c t t t a c a a c t t a t g a g t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c a

4200 AmpR promoter t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g g g g t t a t a a c a g a g t a c t c g c c t a t g t a t a a a c t t a c a t a a a t c t t t t t a t t t g t t t a t c c c c a a

4260 AmpR promoter f1 ori c c g c g c a c a t t t c c c c g a a a a g t g c c a c c t g a c g c g c c c t g t a g c g g c g c a t t a a g c g c g g g c g c g t g t a a a g g g g c t t t t c a c g g t g g a c t g c g c g g g a c a t c g c c g c g t a a t t c g c g c

4320 f1 ori G A F1ori-R g c g g g t g t g g t g g t t a c g c g c a g c g t g a c c g c t a c a c t t g c c a g c g c c c t a g c g c c c g c t c g c c c a c a c c a c c a a t g c g c g t c g c a c t g g c g a t g t g a a c g g t c g c g g g a t c g c g g g c g a

NgoMIVNaeI 4380 f1 ori G G A A A G C G A A A G A A G G G A F1ori-R c c t t t c g c t t t c t t c c c t t c c t t t c t c g c c a c g t t c g c c g g c t t t c c c c g t c a a g c t c t a g g a a a g c g a a a g a a g g g a a g g a a a g a g c g g t g c a a g c g g c c g a a a g g g g c a g t t c g a g a t

4440 f1 ori a a t c g g g g c a t c c c t t t a g g g t t c c g a t t t a g t g c t t t a c g g c a c c t c g a c c c c a a a a a a t t a g c c c c g t a g g g a a a t c c c a a g g c t a a a t c a c g a a a t g c c g t g g a g c t g g g g t t t t t t

DraIII 4500 f1 ori c t t g a t t a g g g t g a t g g t t c a c g t a g t g g g c c a t c g c c c t g a t a g a c g g t t t t t c g c c c t g a a c t a a t c c c a c t a c c a a g t g c a t c a c c c g g t a g c g g g a c t a t c t g c c a a a a a g c g g g a

4560 f1 ori G T G G A C T C T T G T T C C A A A C T G G F1ori-F t t g a c g t t g g a g t c c a c g t t c t t t a a t a g t g g a c t c t t g t t c c a a a c t g g a a c a a c a c t c a a c t g c a a c c t c a g g t g c a a g a a a t t a t c a c c t g a g a a c a a g g t t t g a c c t t g t t g t g a g

PsiI 4620 f1 ori a a c c c t a t c t c g g t c t a t t c t t t t g a t t t a t a a g g g a t t t t g c c g a t t t c g g c c t a t t g g t t g g g a t a g a g c c a g a t a a g a a a a c t a a a t a t t c c c t a a a a c g g c t a a a g c c g g a t a a c c

4680 f1 ori t t a a a a a a t g a g c t g a t t t a a c a a a a a t t t a a c g c g a a t t t t a a c a a a a t a t t a a a c g t t a a t t t t t t a c t c g a c t a a a t t g t t t t t a a a t t g c g c t t a a a a t t g t t t t a t a a t t t g c a a

3ʹ 5ʹ 4688 f1 ori t a c a a t t t a t g t t a a a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.