Addgene pMJ920 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pMJ920 Sequencing Result

Map View

800 600 400 200 End (988) EarI (984) BsaWI (982) XcmI (959) PspFI - PvuII (889) BseYI (885) BmgBI (862) BamHI (743) BseRI (716) EGFP-N (679 .. 700) BtgZI (619) BssSI - BssSαI (560) BtsI - BtsαI (533) PfoI * (459) BsiEI (283) EagI (280) BmrI (140) BsiHKAI (139) Bpu10I (138) BlpI (120) EGFP-C (71 .. 92) BsrGI (31) XbaI (17) ApaI - BanII (15) PspOMI (11) SfcI (1) Start (0) SiriusGFP SV40 NLS SV40 NLS HA HA pMJ920 988 bp

Sequence View

BsrGIPspOMIBanIIApaIXbaIStart (0) SfcI 5ʹ 3ʹ 60 (in frame with SV40 NLS) *KYLEDMGLTIG 235 230 *KYLEDMGLTIG SiriusGFP YRIGPSRLLLVQLVHAESDP *KYLEDMGLTIG c t a t a g a a t a g g g c c c t c t a g a t t a c t a c t t g t a c a g c t c g t c c a t g c c g a g a g t g a t c c g a t a t c t t a t c c c g g g a g a t c t a a t g a t g a a c a t g t c g a g c a g g t a c g g c t c t c a c t a g g

120 (in frame with SV40 NLS) AATVFELLVMHDRKENPDKS 225 220 215 210 AATVFELLVMHDRKENPDKS SiriusGFP G T G C T T G A G G T C G T C C T G G T A C EGFP-C GGGHELQQDHVIALLVGVFA AATVFELLVMHDRKENPDKS c g g c g g c g g t c a c g a a c t c c a g c a g g a c c a t g t g a t c g c g c t t c t c g t t g g g g t c t t t g c g c c g c c g c c a g t g c t t g a g g t c g t c c t g g t a c a c t a g c g c g a a g a g c a a c c c c a g a a a c g

Bpu10IBsiHKAIBmrIBlpI 180 (in frame with SV40 NLS) LKSQTSLYHNDPLLVPGDGI 205 200 195 190 LKSQTSLYHNDPLLVPGDGI SiriusGFP QLGLGAQVVVVGQQHGAVAD LKSQTSLYHNDPLLVPGDGI t c a g c t t g g a c t g g g t g c t c a g g t a g t g g t t g t c g g g c a g c a g c a c g g g g c c g t c g c c g a a g t c g a a c c t g a c c c a c g a g t c c a t c a c c a a c a g c c c g t c g t c g t g c c c c g g c a g c g g c t

240 (in frame with SV40 NLS) PTNQQYHDALQVSGDEVNHR 185 180 175 170 PTNQQYHDALQVSGDEVNHR SiriusGFP GGVLLVVVGELHAAVLDVVA PTNQQYHDALQVSGDEVNHR t g g g g g t g t t c t g c t g g t a g t g g t c g g c g a g c t g c a c g c t g c c g t c c t c g a c g t t g t g g c a c c c c c a c a a g a c g a c c a t c a c c a g c c g c t c g a c g t g c g a c g g c a g g a g c t g c a a c a c c g

EagIBsiEI 300 (in frame with SV40 NLS) IKFNAKIGNKQKDATIYVNH 165 160 155 150 IKFNAKIGNKQKDATIYVNH SiriusGFP DLEVRLDAVLLLVGRDIDVV IKFNAKIGNKQKDATIYVNH g g a t c t t g a a g t t c g c c t t g a t g c c g t t c t t c t g c t t g t c g g c c g t g a t a t a g a c g t t g t c c t a g a a c t t c a a g c g g a a c t a c g g c a a g a a g a c g a a c a g c c g g c a c t a t a t c t g c a a c a

360 (in frame with SV40 NLS) SNFNYELKHGLINGDEKFDI 145 140 135 130 SNFNYELKHGLINGDEKFDI SiriusGFP AVEVVLQLVPQDVAVLLEVD SNFNYELKHGLINGDEKFDI g g c t g t t g a a g t t g t a c t c c a g c t t g t g c c c c a g g a t g t t g c c g t c c t c c t t g a a g t c g a c c g a c a a c t t c a a c a t g a g g t c g a a c a c g g g g t c c t a c a a c g g c a g g a g g a a c t t c a g c t

420 (in frame with SV40 NLS) GKLEIRNVLTDGEFKVEART 125 120 115 110 GKLEIRNVLTDGEFKVEART SiriusGFP ALQLDAVHQGVALELHLGAG GKLEIRNVLTDGEFKVEART t g c c c t t c a g c t c g a t g c g g t t c a c c a g g g t g t c g c c c t c g a a c t t c a c c t c g g c g c g g g a c g g g a a g t c g a g c t a c g c c a a g t g g t c c c a c a g c g g g a g c t t g a a g t g g a g c c g c g c c c

PfoI* 480 (in frame with SV40 NLS) KYTGDDKFSITREQVYGEPM 105 100 95 90 KYTGDDKFSITREQVYGEPM SiriusGFP LVGAVVLEGDGALLDVAFGH KYTGDDKFSITREQVYGEPM t c t t g t a g g t g c c g t c g t c c t t g a a g g a g a t g g t g c g c t c c t g g a c g t a g c c t t c g g g c a a g a a c a t c c a c g g c a g c a g g a a c t t c c t c t a c c a c g c g a g g a c c t g c a t c g g a a g c c c g t

BtsαIBtsI 540 (in frame with SV40 NLS) ASKFFDHQKMHDPYRSFCQV 85 80 75 70 ASKFFDHQKMHDPYRSFCQV SiriusGFP GGLEEVVLLHVVGVAAEALH ASKFFDHQKMHDPYRSFCQV t g g c g g a c t t g a a g a a g t c g t g c t g c t t c a t g t g g t c g g g g t a g c g g c t g a a g c a c t g c a a c c g c c t g a a c t t c t t c a g c a c g a c g a a g t a c a c c a g c c c c a t c g c c g a c t t c g t g a c g t

BssSαIBssSI 600 (in frame with SV40 NLS) GYTLTTVLTPWPVPLKGTTC 65 60 55 50 GYTLTTVLTPWPVPLKGTTC SiriusGFP AVGQGGHEGGPGHGQLAGGA GYTLTTVLTPWPVPLKGTTC c g c c g t a g g t c a g g g t g g t c a c g a g g g t g g g c c a g g g c a c g g g c a g c t t g c c g g t g g t g c g c g g c a t c c a g t c c c a c c a g t g c t c c c a c c c g g t c c c g t g c c c g t c g a a c g g c c a c c a c g

BtgZI 660 (in frame with SV40 NLS) IFKLTLKGNTADGEGEGRVS 45 40 35 30 IFKLTLKGNTADGEGEGRVS SiriusGFP DELQGQLAVGGIALALAAHA IFKLTLKGNTADGEGEGRVS a g a t g a a c t t c a g g g t c a g c t t g c c g t t g g t g g c a t c g c c c t c g c c c t c g c c g c g c a c g c t c t a c t t g a a g t c c c a g t c g a a c g g c a a c c a c c g t a g c g g g a g c g g g a g c g g c g c g t g c g

BseRI 720 (in frame with SV40 NLS) FKHGNVDGDLEVLIPVVGTF 25 20 15 10 FKHGNVDGDLEVLIPVVGTF SiriusGFP C G T C G C C G T C C A G C T C G A C C A G EGFP-N ELVAVYVAVQLDQDGHHPGE FKHGNVDGDLEVLIPVVGTF t g a a c t t g t g g c c g t t t a c g t c g c c g t c c a g c t c g a c c a g g a t g g g c a c c a c c c c g g t g a a c t t g a a c a c c g g c a a a t g c a g c g g c a g g t c g a g c t g g t c c t a c c c g t g g t g g g g c c a c t

BamHI 780 (in frame with HA) SGSSGNSGSGIGD 5 LEEGKS 1a V SiriusGFP (in frame with SV40 NLS) LEEGKSVSGSSGNSGSGIGD QLLALAHGSAAAVAPTTNAV LEEGKSVSGSSGNSGSGIGD a c a g c t c c t c g c c c t t g c t c a c g g a t c c g c t g c t g c c g t t g c t c c c a c t a c c a a t g c c g t t g t c g a g g a g c g g g a a c g a g t g c c t a g g c g a c g a c g g c a a c g a g g g t g a t g g t t a c g g c a

840 SGSG 130 VKRKKKP DE 130 VKRKKKP SV40 NLS SV40 NLS (in frame with HA) VKRKKKPDEVKRKKKPSGSG HFPLFLRIFHLAFFLGGAAA VKRKKKPDEVKRKKKPSGSG c c a c t t t c c g c t t t t t c t t a g g a t c t t c c a c c t t g c g t t t t t t c t t g g g g g a g c c g c t g c g g t g a a a g g c g a a a a a g a a t c c t a g a a g g t g g a a c g c a a a a a a g a a c c c c c t c g g c g a c g

BseYIPvuIIPspFIBmgBI 900 LS 5 1 AYDPVDYPY HA 5 1 LSAYDPVDY HA QAGIIGHVIGIGVASQLRQV LSAYDPVDYPYADGGLQSLD c c a g g c t g g c a t a a t c g g g c a c g t c a t a g g g a t a g g c g t c g c c t c c c a g c t g a g a c a g g t g g t c c g a c c g t a t t a g c c c g t g c a g t a t c c c t a t c c g c a g c g g a g g g t c g a c t c t g t c c a

XcmI 960 DPCLVQAGDALVDQGGVQHL IRTEYLGTISQHILTADLVE c g a t c c g t g t c t c g t a c a g g c c g g t g a t g c t c t g g t g g a t c a g g g t g g c g t c c a g c a c c t g c t a g g c a c a g a g c a t g t c c g g c c a c t a c g a g a c c a c c t a g t c c c a c c g c a g g t c g t g g a

End (988) BsaWIEarI 3ʹ 5ʹ 988 FGAGVPLPV KTSTYRKRD c t t t g g t g c t g g t g t a c c t c t t c c g g t c g a a a c c a c g a c c a c a t g g a g a a g g c c a g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.