Addgene OTOA-bio-His - Sequence Analyzer Skip to main content
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Sequence Analyzer: OTOA-bio-His


Map View

AscI - BssHII (10,019) NsiI (9193) AbsI (9175) SfiI (8876) BmtI (8582) NheI (8578) SphI (8526) SgrAI (8035) BstBI (7439) BsmI (7159) AgeI (7097) EagI - NotI (6620) PmeI (6613) pMT2-F (6572 .. 6591) BsmBI - Esp3I (6386) BlpI (6303) SacI (6070) Eco53kI (6068) LNCX (6067 .. 6091) CMV-F (6021 .. 6041) CMV promoter SnaBI (5842) M13 Reverse (5200 .. 5216) lac operator M13/pUC Reverse (5181 .. 5203) CAP binding site KflI (371) EcoRI (550) XbaI (666) PacI (683) BamHI (687) rbglobpA-R (767 .. 786) BspDI * - ClaI * (835) HpaI (1274) Bsu36I (1400) MluI (2090) pBRforEco (3004 .. 3022) Amp-R (3413 .. 3432) PvuI (3613) FspI (3759) pBR322ori-F (4715 .. 4734) L4440 (4968 .. 4985) BspQI - SapI (4987) OTOA-bio-His 10,025 bp

Sequence View

60 STSITAYKSEGESAEFSFPL g t c g a c c t c c a t c a c g g c c t a t a a g a g t g a g g g g g a g t c a g c g g a g t t c t c c t t c c c a c t c a g c t g g a g g t a g t g c c g g a t a t t c t c a c t c c c c c t c a g t c g c c t c a a g a g g a a g g g t g a

120 NLGEESLQGELRWKAEKAPS c a a c c t t g g a g a g g a a a g c c t g c a g g g a g a g t t g a g a t g g a a g g c a g a g a a g g c t c c t t c g t t g g a a c c t c t c c t t t c g g a c g t c c c t c t c a a c t c t a c c t t c c g t c t c t t c c g a g g a a g

180 SQSWITFSLKNQKVSVQKST t t c c c a g t c c t g g a t c a c c t t c t c c c t a a a g a a c c a a a a g g t g t c t g t g c a g a a g t c t a c a a g g g t c a g g a c c t a g t g g a a g a g g g a t t t c t t g g t t t t c c a c a g a c a c g t c t t c a g a t g

240 SNPKFQLSETLPLTLQIPQV t a g c a a c c c c a a g t t c c a g c t g t c c g a a a c g c t c c c a c t c a c c c t t c a g a t a c c c c a g g t a t c g t t g g g g t t c a a g g t c g a c a g g c t t t g c g a g g g t g a g t g g g a a g t c t a t g g g g t c c a

300 SLQFAGSGNLTLTLDRGILY c t c c c t t c a g t t t g c t g g t t c t g g c a a c c t g a c c c t g a c t c t g g a c a g a g g g a t a c t g t a g a g g g a a g t c a a a c g a c c a a g a c c g t t g g a c t g g g a c t g a g a c c t g t c t c c c t a t g a c a t

360 QEVNLVVMKVTQPDSNTLTC t c a g g a a g t g a a c c t g g t g g t g a t g a a a g t g a c t c a g c c c g a c a g c a a c a c t t t g a c c t g a g t c c t t c a c t t g g a c c a c c a c t a c t t t c a c t g a g t c g g g c t g t c g t t g t g a a a c t g g a c

KflI 420 EVMGPTSPKMRLILKQENQE t g a g g t g a t g g g a c c c a c c t c a c c c a a g a t g a g a c t g a t c t t g a a g c a g g a g a a t c a g g a a c t c c a c t a c c c t g g g t g g a g t g g g t t c t a c t c t g a c t a g a a c t t c g t c c t c t t a g t c c t

480 ARVSRQEKVIQVQAPEAGVW g g c c a g g g t c t c c a g g c a g g a g a a a g t g a t t c a a g t g c a g g c c c c t g a a g c a g g g g t g t g c c g g t c c c a g a g g t c c g t c c t c t t t c a c t a a g t t c a c g t c c g g g g a c t t c g t c c c c a c a c

540 QCLLSEGEEVKMDSKIQVLS g c a a t g t c t a c t g a g t g a a g g t g a a g a g g t c a a g a t g g a c t c c a a g a t c c a g g t t t t a t c c g t t a c a g a t g a c t c a c t t c c a c t t c t c c a g t t c t a c c t g a g g t t c t a g g t c c a a a a t a g

EcoRI 600 KGLNSGSLHHILDAQKMLWN c a a a g g g t t g a a t t c c g g c a g c c t g c a c c a c a t c c t g g a c g c c c a g a a g a t g c t g t g g a a g t t t c c c a a c t t a a g g c c g t c g g a c g t g g t g t a g g a c c t g c g g g t c t t c t a c g a c a c c t t

660 1 5 HHHHHH 6xHis HRDRNLPPLAPLGPHHHHHH c c a c a g g g a c a g g a a c c t g c c t c c t c t g g c c c c t c t g g g c c c c c a c c a c c a t c a c c a c c a g g t g t c c c t g t c c t t g g a c g g a g g a g a c c g g g g a g a c c c g g g g g t g g t g g t a g t g g t g g t

BamHIPacIXbaI 720 * 6xHis β- globin poly(A) signal * t t g a g t c t a g a c a c g t g a t t a a t t a a g g a t c c c c c g a c c t c g a c c t c t g g c t a a t a a a g g a a c t c a g a t c t g t g c a c t a a t t a a t t c c t a g g g g g c t g g a g c t g g a g a c c g a t t a t t t c c

780 β- globin poly(A) signal G T G A G C C T T C C T G T rbglobpA-R a a a t t t a t t t t c a t t g c a a t a g t g t g t t g g a a t t t t t t g t g t c t c t c a c t c g g a a g g a c a t t t a a a t a a a a g t a a c g t t a t c a c a c a a c c t t a a a a a a c a c a g a g a g t g a g c c t t c c t g t

BspDI*ClaI* 840 A T A C C C rbglobpA-R t a t g g g a g g g c a a a t c a t t t g g t c g a g a t c c c t c g g a g a t c t c t a g c t a g a g g a t c g a t c a t a c c c t c c c g t t t a g t a a a c c a g c t c t a g g g a g c c t c t a g a g a t c g a t c t c c t a g c t a g

900 c c c g c c c c g g a c g a a c t a a a c c t g a c t a c g a c a t c t c t g c c c c t t c t t c g c g g g g c a g t g g g g c g g g g c c t g c t t g a t t t g g a c t g a t g c t g t a g a g a c g g g g a a g a a g c g c c c c g t c a c

960 c a t g t a a t c c c t t c a g t t g g t t g g t a c a a c t t g c c a a c t g g g c c c t g t t c c a c a t g t g a c g t a c a t t a g g g a a g t c a a c c a a c c a t g t t g a a c g g t t g a c c c g g g a c a a g g t g t a c a c t g

1020 a c g g g g g g g g a c c a a a c a c a a a g g g g t t c t c t g a c t g t a g t t g a c a t c c t t a t a a a t g g a t g c c c c c c c c t g g t t t g t g t t t c c c c a a g a g a c t g a c a t c a a c t g t a g g a a t a t t t a c c t

1080 t g t g c a c a t t t g c c a a c a c t g a g t g g c t t t c a t c c t g g a g c a g a c t t t g c a g t c t g t g g a a c a c g t g t a a a c g g t t g t g a c t c a c c g a a a g t a g g a c c t c g t c t g a a a c g t c a g a c a c c t

1140 c t g c a a c a c a a c a t t g c c t t t a t g t g t a a c t c t t g g c t g a a g c t c t t a c a c c a a t g c t g g g a c g t t g t g t t g t a a c g g a a a t a c a c a t t g a g a a c c g a c t t c g a g a a t g t g g t t a c g a c c

1200 g g g a c a t g t a c c t c c c a g g g g c c c a g g a a g a c t a c g g g a g g c t a c a c c a a c g t c a a t c a g c c c t g t a c a t g g a g g g t c c c c g g g t c c t t c t g a t g c c c t c c g a t g t g g t t g c a g t t a g t c

1260 a g g g g c c t g t g t a g c t a c c g a t a a g c g g a c c c t c a a g a g g g c a t t a g c a a t a g t g t t t a t t c c c c g g a c a c a t c g a t g g c t a t t c g c c t g g g a g t t c t c c c g t a a t c g t t a t c a c a a a t a

HpaI 1320 a a g g c c c c c t t g t t a a c c c t a a a c g g g t a g c a t a t g c t t c c c g g g t a g t a g t a t a t a c t a t t c c g g g g g a a c a a t t g g g a t t t g c c c a t c g t a t a c g a a g g g c c c a t c a t c a t a t a t g a t

1380 t c c a g a c t a a c c c t a a t t c a a t a g c a t a t g t t a c c c a a c g g g a a g c a t a t g c t a t c g a a t a g g t c t g a t t g g g a t t a a g t t a t c g t a t a c a a t g g g t t g c c c t t c g t a t a c g a t a g c t t a

Bsu36I 1440 t a g g g t t a g t a a a a g g g t c c t a a g g a a c a g c g a t a t c t c c c a c c c c a t g a g c t g t c a c g g a t c c c a a t c a t t t t c c c a g g a t t c c t t g t c g c t a t a g a g g g t g g g g t a c t c g a c a g t g c c

1500 t t t t a t t t a c a t g g g g t c a g g a t t c c a c g a g g g t a g t g a a c c a t t t t a g t c a c a a g g g c a a a a a t a a a t g t a c c c c a g t c c t a a g g t g c t c c c a t c a c t t g g t a a a a t c a g t g t t c c c g t

1560 g t g g c t g a a g a t c a a g g a g c g g g c a g t g a a c t c t c c t g a a t c t t c g c c t g c t t c t t c a t t c a c c g a c t t c t a g t t c c t c g c c c g t c a c t t g a g a g g a c t t a g a a g c g g a c g a a g a a g t a a

1620 c t c c t t c g t t t a g c t a a t a g a a t a a c t g c t g a g t t g t g a a c a g t a a g g t g t a t g t g a g g t g a g g a a g c a a a t c g a t t a t c t t a t t g a c g a c t c a a c a c t t g t c a t t c c a c a t a c a c t c c a

1680 g c t c g a a a a c a a g g t t t c a g g t g a c g c c c c c a g a a t a a a a t t t g g a c g g g g g g t t c a g t g c g a g c t t t t g t t c c a a a g t c c a c t g c g g g g g t c t t a t t t t a a a c c t g c c c c c c a a g t c a c

1740 g t g g c a t t g t g c t a t g a c a c c a a t a t a a c c c t c a c a a a c c c c t t g g g c a a t a a a t a c t a g c a c c g t a a c a c g a t a c t g t g g t t a t a t t g g g a g t g t t t g g g g a a c c c g t t a t t t a t g a t c

1800 t g t a g g a a t g a a a c a t t c t g a a t a t c t t t a a c a a t a g a a a t c c a t g g g g t g g g g a c a a g c a c a t c c t t a c t t t g t a a g a c t t a t a g a a a t t g t t a t c t t t a g g t a c c c c a c c c c t g t t c g

1860 c g t a a a g a c t g g a t g t c c a t c t c a c a c g a a t t t a t g g c t a t g g g c a a c a c a t a a t c c t a g g c a t t t c t g a c c t a c a g g t a g a g t g t g c t t a a a t a c c g a t a c c c g t t g t g t a t t a g g a t c

1920 t g c a a t a t g a t a c t g g g g t t a t t a a g a t g t g t c c c a g g c a g g g a c c a a g a c a g g t g a a c c a c g t t a t a c t a t g a c c c c a a t a a t t c t a c a c a g g g t c c g t c c c t g g t t c t g t c c a c t t g g

1980 a t g t t g t t a c a c t c t a t t t g t a a c a a g g g g a a a g a g a g t g g a c g c c g a c a g c a g c g g a c t t a c a a c a a t g t g a g a t a a a c a t t g t t c c c c t t t c t c t c a c c t g c g g c t g t c g t c g c c t g a

2040 c c a c t g g t t g t c t c t a a c a c c c c c g a a a a t t a a a c g g g g c t c c a c g c c a a t g g g g c c c a t g g t g a c c a a c a g a g a t t g t g g g g g c t t t t a a t t t g c c c c g a g g t g c g g t t a c c c c g g g t a

MluI 2100 a a a c a a a g a c a a g t g g c c a c t c t t t t t t t t g a a a t t g t g g a g t g g g g g c a c g c g t c a g c c t t t g t t t c t g t t c a c c g g t g a g a a a a a a a a c t t t a a c a c c t c a c c c c c g t g c g c a g t c g g

2160 c c c a c a c g c c g c c c t g c g g t t t t g g a c t g t a a a a t a a g g g t g t a a t a a c t t g g c t g a t t g g g g t g t g c g g c g g g a c g c c a a a a c c t g a c a t t t t a t t c c c a c a t t a t t g a a c c g a c t a a c

2220 t a a c c c c g c t a a c c a c t g c g g t c a a a c c a c t t g c c c a c a a a a c c a c t a a t g g c a c c c c g g a t t g g g g c g a t t g g t g a c g c c a g t t t g g t g a a c g g g t g t t t t g g t g a t t a c c g t g g g g c c

2280 g g a a t a c c t g c a t a a g t a g g t g g g c g g g c c a a g a t a g g g g c g c g a t t g c t g c g a t c t g g a c c t t a t g g a c g t a t t c a t c c a c c c g c c c g g t t c t a t c c c c g c g c t a a c g a c g c t a g a c c t

2340 g g a c a a a t t a c a c a c a c t t g c g c c t g a g c g c c a a g c a c a g g g t t g t t g g t c c t c a t a t t c c c t g t t t a a t g t g t g t g a a c g c g g a c t c g c g g t t c g t g t c c c a a c a a c c a g g a g t a t a a g

2400 a c g a g g t c g c t g a g a g c a c g g t g g g c t a a t g t t g c c a t g g g t a g c a t a t a c t a c c c a a a t t g c t c c a g c g a c t c t c g t g c c a c c c g a t t a c a a c g g t a c c c a t c g t a t a t g a t g g g t t t a

2460 a t c t g g a t a g c a t a t g c t a t c c t a a t c t a t a t c t g g g t a g c a t a g g c t a t c c t a a t c t a t t a g a c c t a t c g t a t a c g a t a g g a t t a g a t a t a g a c c c a t c g t a t c c g a t a g g a t t a g a t a

2520 a t c t g g g t a g c a t a t g c t a t c c t a a t c t a t a t c t g g g t a g t a t a t g c t a t c c t a a t t t a t t a g a c c c a t c g t a t a c g a t a g g a t t a g a t a t a g a c c c a t c a t a t a c g a t a g g a t t a a a t a

2580 a t c t g g g t a g c a t a g g c t a t c c t a a t c t a t a t c t g g g t a g c a t a t g c t a t c c t a a t c t a t t a g a c c c a t c g t a t c c g a t a g g a t t a g a t a t a g a c c c a t c g t a t a c g a t a g g a t t a g a t a

2640 a t c t g g g t a g t a t a t g c t a t c c t a a t c t g t a t c c g g g t a g c a t a t g c t a t c c t a a t a g a g t a g a c c c a t c a t a t a c g a t a g g a t t a g a c a t a g g c c c a t c g t a t a c g a t a g g a t t a t c t c

2700 a t t a g g g t a g t a t a t g c t a t c c t a a t t t a t a t c t g g g t a g c a t a t a c t a c c c a a a t a t c t t a a t c c c a t c a t a t a c g a t a g g a t t a a a t a t a g a c c c a t c g t a t a t g a t g g g t t t a t a g a

2760 g g a t a g c a t a t g c t a t c c t a a t c t a t a t c t g g g t a g c a t a t g c t a t c c t a a t c t a t a t c t c c t a t c g t a t a c g a t a g g a t t a g a t a t a g a c c c a t c g t a t a c g a t a g g a t t a g a t a t a g a

2820 g g g t a g c a t a g g c t a t c c t a a t c t a t a t c t g g g t a g c a t a t g c t a t c c t a a t c t a t a t c t c c c a t c g t a t c c g a t a g g a t t a g a t a t a g a c c c a t c g t a t a c g a t a g g a t t a g a t a t a g a

2880 g g g t a g t a t a t g c t a t c c t a a t t t a t a t c t g g g t a g c a t a g g c t a t c c t a a t c t a t a t c t c c c a t c a t a t a c g a t a g g a t t a a a t a t a g a c c c a t c g t a t c c g a t a g g a t t a g a t a t a g a

2940 g g g t a g c a t a t g c t a t c c t a a t c t a t a t c t g g g t a g t a t a t g c t a t c c t a a t c t g t a t c c c c c a t c g t a t a c g a t a g g a t t a g a t a t a g a c c c a t c a t a t a c g a t a g g a t t a g a c a t a g g

3000 g g g t a g c a t a t g c t a t c c t c a t g a t a a g c t g t c a a a c a t g a g a a t t t t c t t g a a g a c g a a c c c a t c g t a t a c g a t a g g a g t a c t a t t c g a c a g t t t g t a c t c t t a a a a g a a c t t c t g c t t

3060 C G G A G C A C T A T G C G G A T A A pBRforEco a g g g c c t c g t g a t a c g c c t a t t t t t a t a g g t t a a t g t c a t g a t a a t a a t g g t t t c t t a g a t c c c g g a g c a c t a t g c g g a t a a a a a t a t c c a a t t a c a g t a c t a t t a t t a c c a a a g a a t c t

3120 AmpR promoter c g t c a g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a g c a g t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t

3180 AmpR promoter t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a

3240 1 5 10 15 signal sequence MSIQHFRVALIPFFA AmpR promoter AmpR MSIQHFRVALIPFFA g a a a a a g g a a g a g t a t g a g t a t t c a a c a t t t c c g t g t c g c c c t t a t t c c c t t t t t t g c g g c t t t t t c c t t c t c a t a c t c a t a a g t t g t a a a g g c a c a g c g g g a a t a a g g g a a a a a a c g c c

3300 20 signal sequence AFCLPVFA 25 30 35 HPETLVKVKDAE AmpR AFCLPVFAHPETLVKVKDAE c a t t t t g c c t t c c t g t t t t t g c t c a c c c a g a a a c g c t g g t g a a a g t a a a a g a t g c t g a a g g t a a a a c g g a a g g a c a a a a a c g a g t g g g t c t t t g c g a c c a c t t t c a t t t t c t a c g a c t t c

3360 40 45 50 55 DQLGARVGYIELDLNSGKIL AmpR DQLGARVGYIELDLNSGKIL a t c a g t t g g g t g c a c g a g t g g g t t a c a t c g a a c t g g a t c t c a a c a g c g g t a a g a t c c t t g t a g t c a a c c c a c g t g c t c a c c c a a t g t a g c t t g a c c t a g a g t t g t c g c c a t t c t a g g a a c

3420 60 65 70 75 ESFRPEERFPMMSTFKVLLC AmpR C G A T A C A C Amp-R ESFRPEERFPMMSTFKVLLC a g a g t t t t c g c c c c g a a g a a c g t t t t c c a a t g a t g a g c a c t t t t a a a g t t c t g c t a t g t g t c t c a a a a g c g g g g c t t c t t g c a a a a g g t t a c t a c t c g t g a a a a t t t c a a g a c g a t a c a c

3480 80 85 90 95 GAVLSRVDAGQEQLGRRIHY AmpR C G C G C C A T A A T A Amp-R GAVLSRVDAGQEQLGRRIHY g c g c g g t a t t a t c c c g t g t t g a c g c c g g g c a a g a g c a a c t c g g t c g c c g c a t a c a c t a t t c g c g c c a t a a t a g g g c a c a a c t g c g g c c c g t t c t c g t t g a g c c a g c g g c g t a t g t g a t a a

3540 100 105 110 115 SQNDLVEYSPVTEKHLTDGM AmpR SQNDLVEYSPVTEKHLTDGM c t c a g a a t g a c t t g g t t g a g t a c t c a c c a g t c a c a g a a a a g c a t c t t a c g g a t g g c a t g a g a g t c t t a c t g a a c c a a c t c a t g a g t g g t c a g t g t c t t t t c g t a g a a t g c c t a c c g t a c t

3600 120 125 130 135 TVRELCSAAITMSDNTAANL AmpR TVRELCSAAITMSDNTAANL c a g t a a g a g a a t t a t g c a g t g c t g c c a t a a c c a t g a g t g a t a a c a c t g c g g c c a a c t t a c g t c a t t c t c t t a a t a c g t c a c g a c g g t a t t g g t a c t c a c t a t t g t g a c g c c g g t t g a a t g

PvuI 3660 140 145 150 155 LLTTIGGPKELTAFLHNMGD AmpR LLTTIGGPKELTAFLHNMGD t t c t g a c a a c g a t c g g a g g a c c g a a g g a g c t a a c c g c t t t t t t g c a c a a c a t g g g g g a t c a a g a c t g t t g c t a g c c t c c t g g c t t c c t c g a t t g g c g a a a a a a c g t g t t g t a c c c c c t a g

3720 160 165 170 175 HVTRLDRWEPELNEAIPNDE AmpR HVTRLDRWEPELNEAIPNDE a t g t a a c t c g c c t t g a t c g t t g g g a a c c g g a g c t g a a t g a a g c c a t a c c a a a c g a c g a g c t a c a t t g a g c g g a a c t a g c a a c c c t t g g c c t c g a c t t a c t t c g g t a t g g t t t g c t g c t c g

FspI 3780 180 185 190 195 RDTTMPAAMATTLRKLLTGE AmpR RDTTMPAAMATTLRKLLTGE g t g a c a c c a c g a t g c c t g c a g c a a t g g c a a c a a c g t t g c g c a a a c t a t t a a c t g g c g a a c c a c t g t g g t g c t a c g g a c g t c g t t a c c g t t g t t g c a a c g c g t t t g a t a a t t g a c c g c t t g

3840 200 205 210 215 LLTLASRQQLIDWMEADKVA AmpR LLTLASRQQLIDWMEADKVA t a c t t a c t c t a g c t t c c c g g c a a c a a t t a a t a g a c t g g a t g g a g g c g g a t a a a g t t g c a g a t g a a t g a g a t c g a a g g g c c g t t g t t a a t t a t c t g a c c t a c c t c c g c c t a t t t c a a c g t c

3900 220 225 230 235 GPLLRSALPAGWFIADKSGA AmpR GPLLRSALPAGWFIADKSGA g a c c a c t t c t g c g c t c g g c c c t t c c g g c t g g c t g g t t t a t t g c t g a t a a a t c t g g a g c c g c t g g t g a a g a c g c g a g c c g g g a a g g c c g a c c g a c c a a a t a a c g a c t a t t t a g a c c t c g g c

3960 240 245 250 255 GERGSRGIIAALGPDGKPSR AmpR GERGSRGIIAALGPDGKPSR g t g a g c g t g g g t c t c g c g g t a t c a t t g c a g c a c t g g g g c c a g a t g g t a a g c c c t c c c g t a c a c t c g c a c c c a g a g c g c c a t a g t a a c g t c g t g a c c c c g g t c t a c c a t t c g g g a g g g c a t

4020 260 265 270 275 IVVIYTTGSQATMDERNRQI AmpR IVVIYTTGSQATMDERNRQI t c g t a g t t a t c t a c a c g a c g g g g a g t c a g g c a a c t a t g g a t g a a c g a a a t a g a c a g a t c g a g c a t c a a t a g a t g t g c t g c c c c t c a g t c c g t t g a t a c c t a c t t g c t t t a t c t g t c t a g c

4080 280 285 AEIGASLIKHW* AmpR AEIGASLIKHW* c t g a g a t a g g t g c c t c a c t g a t t a a g c a t t g g t a a c t g t c a g a c c a a g t t t a c t c a t a t a g a c t c t a t c c a c g g a g t g a c t a a t t c g t a a c c a t t g a c a g t c t g g t t c a a a t g a g t a t a t

4140 t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a

4200 t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g

4260 ori c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a

4320 ori t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t

4380 ori c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t t c t t c t a g g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a a g a a g a t c

4440 ori t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g

4500 ori t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c

4560 ori a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t

4620 ori c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a

4680 ori g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c

4740 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g

4800 ori c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g

4860 ori g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g

4920 c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c

4980 A G C G A G T C A G T G A L4440 c c t t t g a g t g a g c t g a t a c c g c t c g c c g c a g c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g g a a a c t c a c t c g a c t a t g g c g a g c g g c g t c g g c t t g c t g g c t c g c g t c g c t c a g t c a c t

BspQISapI 5040 G C G A G L4440 g c g a g g a a g c g g a a g a g c g c c c a a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c c g c t c c t t c g c c t t c t c g c g g g t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g

5100 a t t a a t g c a g c t g g c a c g a c a g g t t t c c c g a c t g g a a a g c g g g c a g t g a g c g c a a c g c a a t a a t t a c g t c g a c c g t g c t g t c c a a a g g g c t g a c c t t t c g c c c g t c a c t c g c g t t g c g t t

5160 -35 CAP binding site lac promoter t t a a t g t g a g t t a g c t c a c t c a t t a g g c a c c c c a g g c t t t a c a c t t t a t g c t t c c g g c t c a a t t a c a c t c a a t c g a g t g a g t a a t c c g t g g g g t c c g a a a t g t g a a a t a c g a a g g c c g a g

5220 -10 lac promoter lac operator M13 rev A G C G G A T A A C A A T T T C A C A C A G G M13/pUC Reverse C A G G A A A C A G C T A T G A C M13 Reverse g t a t g t t g t g t g g a a t t g t g a g c g g a t a a c a a t t t c a c a c a g g a a a c a g c t a t g a c c a t g c a t a c a a c a c a c c t t a a c a c t c g c c t a t t g t t a a a g t g t g t c c t t t g t c g a t a c t g g t a c

5280 a t t a c g c c a a g c t c t a g c t a g a g g t c g a c c a a t t c t c a t g t t t g a c a g c t t a t c a t c g c a t a a t g c g g t t c g a g a t c g a t c t c c a g c t g g t t a a g a g t a c a a a c t g t c g a a t a g t a g c g t

5340 g a t c c g g g c a a c g t t g t t g c c a t t g c t g c a g g c g c a g a a c t g g t a g g t a t g g a a g a t c t a c t a g g c c c g t t g c a a c a a c g g t a a c g a c g t c c g c g t c t t g a c c a t c c a t a c c t t c t a g a t

5400 t a c a t t g a a t c a a t a t t g g c a a t t a g c c a t a t t a g t c a t t g g t t a t a t a g c a t a a a t c a a a t g t a a c t t a g t t a t a a c c g t t a a t c g g t a t a a t c a g t a a c c a a t a t a t c g t a t t t a g t t

5460 t a t t g g c t a t t g g c c a t t g c a t a c g t t g t a t c t a t a t c a t a a t a t g t a c a t t t a t a t t g g a t a a c c g a t a a c c g g t a a c g t a t g c a a c a t a g a t a t a g t a t t a t a c a t g t a a a t a t a a c c

5520 CMV enhancer c t c a t g t c c a a t a t g a c c g c c a t g t t g a c a t t g a t t a t t g a c t a g t t a t t a a t a g t a a t c g a g t a c a g g t t a t a c t g g c g g t a c a a c t g t a a c t a a t a a c t g a t c a a t a a t t a t c a t t a g

5580 CMV enhancer a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a t a t g g a g t t c c g c g t t a c a t a a c t t a c g g t t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t a t a c c t c a a g g c g c a a t g t a t t g a a t g c c a

5640 CMV enhancer a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c c c c g c c c a t t g a c g t c a a t a a t g a c g t a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g g g g c g g g t a a c t g c a g t t a t t a c t g c a t

5700 CMV enhancer t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c a t t g a c g t c a a t g g g t g g a g t a t t t a c g a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g t a a c t g c a g t t a c c c a c c t c a t a a a t g c

5760 CMV enhancer g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t a t c a t a t g c c a a g t c c g c c c c c t a t t g a c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a t a g t a t a c g g t t c a g g c g g g g g a t a a c t

5820 CMV enhancer c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t a t g c c c a g t a c a t g a c c t t a c g g g a c t t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a t a c g g g t c a t g t a c t g g a a t g c c c t g a a

SnaBI 5880 CMV enhancer CMV promoter t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t c g c t a t t a c c a t g g t g a t g c g g t t t t g a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t a g c g a t a a t g g t a c c a c t a c g c c a a a a c

5940 CMV promoter g c a g t a c a c c a a t g g g c g t g g a t a g c g g t t t g a c t c a c g g g g a t t t c c a a g t c t c c a c c c c g t c a t g t g g t t a c c c g c a c c t a t c g c c a a a c t g a g t g c c c c t a a a g g t t c a g a g g t g g g

6000 CMV promoter c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c a a a a t c a a c g g g a c t t t c c a a a a t g t c g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g t t t t a g t t g c c c t g a a a g g t t t t a c a g c

6060 CMV promoter C G C A A A T G G G C G G T A G G C G T G CMV-F t a a t a a c c c c g c c c c g t t g a c g c a a a t g g g c g g t a g g c g t g t a c g g t g g g a g g t c t a t a t a t t a t t g g g g c g g g g c a a c t g c g t t t a c c c g c c a t c c g c a c a t g c c a c c c t c c a g a t a t a

Eco53kISacI 6120 CMV promoter A G C T C G T T T A G T G A A C C G T C A G A T C LNCX a a g c a g a g c t c g t t t a g t g a a c c g t c a g a t c c t c a c t c t c t t c c g c a t c g c t g t c t g c g a t t c g t c t c g a g c a a a t c a c t t g g c a g t c t a g g a g t g a g a g a a g g c g t a g c g a c a g a c g c t

6180 g g g c c a g c t g t t g g g c t c g c g g t t g a g g a c a a a c t c t t c g c g g t c t t t c c a g t a c t c t t g c c c g g t c g a c a a c c c g a g c g c c a a c t c c t g t t t g a g a a g c g c c a g a a a g g t c a t g a g a a c

6240 g a t c g g a a a c c c g t c g g c c t c c g a a c g g t a c t c c g c c a c c g a g g g a c c t g a g c g a g t c c g c t a g c c t t t g g g c a g c c g g a g g c t t g c c a t g a g g c g g t g g c t c c c t g g a c t c g c t c a g g c

6300 c a t c g a c c g g a t c g g a a a a c c t c t c g a g a a a g g c g t c t a a c c a g t c a c a g t c g c a a g g t a g t a g c t g g c c t a g c c t t t t g g a g a g c t c t t t c c g c a g a t t g g t c a g t g t c a g c g t t c c a t

BlpI 6360 g g c t g a g c a c c g t g g c g g g c g g c a g c g g g t g g c g g t c g g g g t t g t t t c t g g c g g a g g t g c c c g a c t c g t g g c a c c g c c c g c c g t c g c c c a c c g c c a g c c c c a a c a a a g a c c g c c t c c a c g

BsmBIEsp3I 6420 t g c t g a t g a t g t a a t t a a a g t a g g c g g t c t t g a g a c g g c g g a t g g t c g a g g t g a g g t g t g a c g a c t a c t a c a t t a a t t t c a t c c g c c a g a a c t c t g c c g c c t a c c a g c t c c a c t c c a c a c

6480 g c a g g c t t g a g a t c c a g c t g t t g g g g t g a g t a c t c c c t c t c a a a a g c g g g c a t t a c t t c t c g t c c g a a c t c t a g g t c g a c a a c c c c a c t c a t g a g g g a g a g t t t t c g c c c g t a a t g a a g a

6540 *NGFVLLIQYEGPGIQ g c g c t a a g a t t g t c a g t t t c c a a a a a c g a g g a g g a t t t g a t a t t c a c c t g g c c c g a t c t g c g c g a t t c t a a c a g t c a a a g g t t t t t g c t c c t c c t a a a c t a t a a g t g g a c c g g g c t a g a c

6600 T T G C C T T T C T C T C C A C A G G T pMT2-F GYVQTVIVDVKGKEGCTDVG g c c a t a c a c t t g a g t g a c a a t g a c a t c c a c t t t g c c t t t c t c t c c a c a g g t g t c c a c t c c c g g t a t g t g a a c t c a c t g t t a c t g t a g g t g a a a c g g a a a g a g a g g t g t c c a c a g g t g a g g

EagINotIPmeI 6660 Kozak sequence MVQEPTTYSL LDLNLSRGGGHHLFGGGVAQ c a g g t c c a a g t t t a a a c t g c g g c c g c c a c c a t g g t g c a g g a a c c c a c c a c c t a c a g c c t g g t c c a g g t t c a a a t t t g a c g c c g g c g g t g g t a c c a c g t c c t t g g g t g g t g g a t g t c g g a c

6720 FLFLFLSHGVSSYTVPNSRQ KQEQKQAVAHAAVGHGVASL t t t c t g t t c c t g t t t c t g a g c c a c g g c g t g a g c a g c t a c a c c g t g c c c a a c a g c a g a c a g a a a g a c a a g g a c a a a g a c t c g g t g c c g c a c t c g t c g a t g t g g c a c g g g t t g t c g t c t g t c

6780 DLHPLLQNMAEEIIDGSYLN IQVGQQLVHGLFDDVAAVQV g a t c t g c a c c c c c t g c t g c a g a a c a t g g c c g a g g a a a t c a t c g a c g g c a g c t a c c t g a a c c t a g a c g t g g g g g a c g a c g t c t t g t a c c g g c t c c t t t a g t a g c t g c c g t c g a t g g a c t t g

6840 ALLDLIQFQSSHVWTDDLSH GQQVQDLKLAAVHPGVVQAV g c c c t g c t g g a c c t g a t c c a g t t t c a g a g c a g c c a c g t g t g g a c c g a c g a c c t g a g c c a c c g g g a c g a c c t g g a c t a g g t c a a a g t c t c g t c g g t g c a c a c c t g g c t g c t g g a c t c g g t g

6900 RVLAYLNSRNVAFTIPSLQA SHQGVQVAPVHGEGDWRQLG *RFLRFTAKVMGDRCA a g a g t g c t g g c c t a c c t g a a c a g c c g g a a c g t g g c c t t c a c c a t c c c a t c t c t g c a g g c c t c t c a c g a c c g g a t g g a c t t g t c g g c c t t g c a c c g g a a g t g g t a g g g t a g a g a c g t c c g g

6960 AVENHLEQRLHQPQKLLEDL GHLVVQFLPQVLGLLQQFIQ ATSFWRSCRSCWGWFSSSSR g c c g t g g a g a a c c a c c t g g a a c a g c g g c t g c a c c a g c c c c a g a a g c t g c t g g a a g a t c t g c g g c a c c t c t t g g t g g a c c t t g t c g c c g a c g t g g t c g g g g t c t t c g a c g a c c t t c t a g a c

7020 RKTDAQQFRTAMKCLLEDKK SLGVGLLEPGGHLAQELVLL LFVSAWCNRVAMFHRSSSLF a g a a a g a c c g a c g c c c a g c a g t t c c g g a c c g c c a t g a a g t g c c t g c t c g a g g a c a a g a a g t c t t t c t g g c t g c g g g t c g t c a a g g c c t g g c g g t a c t t c a c g g a c g a g c t c c t g t t c t t c

7080 DGLDLKDIIIDLGEIRERAL VAQVQLVDDDVQALDSLSGQ SPRSRFSMMMSRPSILSLAR g a c g g c c t g g a c c t g a a g g a c a t c a t c a t c g a c c t g g g c g a g a t c a g a g a g a g a g c c c t g c t g c c g g a c c t g g a c t t c c t g t a g t a g t a g c t g g a c c c g c t c t a g t c t c t c t c t c g g g a c

AgeI 7140 QSPGVNRSLFLITLERCFQM LARAHVPGQEQDGQFPAELH CLGPTFRDRNRIVRSRHKWI c a g a g c c c t g g c g t g a a c c g g t c c c t g t t c c t g a t c a c c c t g g a a c g g t g c t t c c a g a t g g t c t c g g g a c c g c a c t t g g c c a g g g a c a a g g a c t a g t g g g a c c t t g c c a c g a a g g t c t a c

BsmI 7200 LNSLECVEILGKVLRGSSGS QVGQFAHLDQALHQSAAAAA SFERSHTSIRPLTSLPLLPL c t g a a c t c c c t g g a a t g c g t g g a g a t c c t g g g c a a g g t g c t g a g a g g c a g c a g c g g c a g c g a c t t g a g g g a c c t t a c g c a c c t c t a g g a c c c g t t c c a c g a c t c t c c g t c g t c g c c g t c g

7260 FLQPDITERLPRDLREDAFK EQLGVDGLSQGPVQSLVGEL KRCGSMVSLSGRSRLSSAKL t t c c t g c a g c c c g a c a t c a c c g a g a g a c t g c c c c g g g a c c t g a g a g a g g a c g c c t t c a a g a a g g a c g t c g g g c t g t a g t g g c t c t c t g a c g g g g c c c t g g a c t c t c t c c t g c g g a a g t t c

7320 NLSAVFKDLYDKTSAHSQRA VQAGHELVQVVLGAGVALPG FRLATNLSRYSLVLAWLWLA a a c c t g a g c g c c g t g t t c a a g g a c c t g t a c g a c a a g a c c a g c g c c c a c a g c c a g a g g g c c t t g g a c t c g c g g c a c a a g t t c c t g g a c a t g c t g t t c t g g t c g c g g g t g t c g g t c t c c c g g

7380 LYSWMTGILQTSSNATDDSA QVAPHCADQLGAAVGGVIAG RYLQIVPMRCVLLLAVSSLA c t g t a c a g c t g g a t g a c a g g c a t c c t g c a g a c c a g c a g c a a c g c c a c c g a c g a t a g c g c c g a c a t g t c g a c c t a c t g t c c g t a g g a c g t c t g g t c g t c g t t g c g g t g g c t g c t a t c g c g g

BstBI 7440 SWVSAEHLWVLGRYMVHLSF RPHGGLVQPHQAPVHHVQAE EQTDASCRHTSPRYMTCRLK t c t t g g g t g t c c g c c g a g c a c c t g t g g g t g c t g g g c c g g t a c a t g g t g c a c c t g a g c t t c a g a a c c c a c a g g c g g c t c g t g g a c a c c c a c g a c c c g g c c a t g t a c c a c g t g g a c t c g a a g

7500 EEITKISPIEIGLFISYDNA FLDGLDAGDLDAQEDAVVVG SSIVLILGMSIPRNM g a a g a g a t c a c c a a g a t c a g c c c c a t c g a g a t c g g c c t g t t c a t c a g c t a c g a c a a c g c c c t t c t c t a g t g g t t c t a g t c g g g g t a g c t c t a g c c g g a c a a g t a g t c g a t g c t g t t g c g g

7560 TKQLDMVYDITPELAQAFLE GLLQVHHVVDGRLQGLGEQF a c c a a g c a g c t g g a c a t g g t g t a c g a c a t c a c c c c t g a g c t g g c c c a g g c c t t c c t g g a a t g g t t c g t c g a c c t g t a c c a c a t g c t g t a g t g g g g a c t c g a c c g g g t c c g g a a g g a c c t t

7620 RISSSNFNMRNTSTIHRQAH PDAAAVEVHPVGAGDVSLSV c g g a t c a g c a g c a g c a a c t t c a a c a t g c g g a a c a c c a g c a c c a t c c a c a g a c a g g c t c a c g c c t a g t c g t c g t c g t t g a a g t t g t a c g c c t t g t g g t c g t g g t a g g t g t c t g t c c g a g t g

7680 ELWALEPFPKMLGLLVCFYN LQPGQFGEGLHQAQEHAEVV g a g c t g t g g g c c c t g g a a c c c t t c c c c a a g a t g c t g g g c c t g c t c g t g t g c t t c t a c a a c c t c g a c a c c c g g g a c c t t g g g a a g g g g t t c t a c g a c c c g g a c g a g c a c a c g a a g a t g t t g

7740 DLELLDATVAQVLLYQMIKC VQFQQIGGHGLHQQVLHDLA g a c c t g g a a c t g c t g g a t g c c a c c g t g g c c c a g g t g c t g c t g t a c c a g a t g a t c a a g t g c c t g g a c c t t g a c g a c c t a c g g t g g c a c c g g g t c c a c g a c g a c a t g g t c t a c t a g t t c a c g

7800 SHLRGFQAGVQKLKAELLDI AVQSAKLGAHLLQLGLQQID a g c c a c c t g a g a g g c t t t c a g g c c g g c g t g c a g a a g c t g a a g g c c g a g c t g c t g g a t a t c t c g g t g g a c t c t c c g a a a g t c c g g c c g c a c g t c t t c g a c t t c c g g c t c g a c g a c c t a t a g

7860 AMENQTLNETLGSLSDAVVG GHFVLGQVLCQAAQRIGDHA g c c a t g g a a a a c c a g a c c c t g a a c g a g a c a c t g g g c a g c c t g t c t g a t g c c g t c g t g g g c c g g t a c c t t t t g g t c t g g g a c t t g c t c t g t g a c c c g t c g g a c a g a c t a c g g c a g c a c c c g

7920 LTYSQLESLSPEAVHGAIST QGVALQFAQARFGDMAGDAG c t g a c c t a c a g c c a g c t g g a a a g c c t g a g c c c t g a a g c c g t c c a t g g c g c c a t c a g c a c c g a c t g g a t g t c g g t c g a c c t t t c g g a c t c g g g a c t t c g g c a g g t a c c g c g g t a g t c g t g g

7980 LNQVSGWAKSQVIILSAKYL QVLHGAPGLALDDDQAGLVQ c t g a a c c a g g t g t c c g g c t g g g c c a a g a g c c a g g t c a t c a t c c t g a g c g c c a a g t a c c t g g a c t t g g t c c a c a g g c c g a c c c g g t t c t c g g t c c a g t a g t a g g a c t c g c g g t t c a t g g a c

SgrAI 8040 AHEKVLSFYNVSQMGALLAG GVLLHQGEVIHGLHSGQEGT g c c c a c g a g a a g g t g c t g t c c t t c t a c a a t g t g t c c c a g a t g g g a g c c c t g c t c g c c g g t c g g g t g c t c t t c c a c g a c a g g a a g a t g t t a c a c a g g g t c t a c c c t c g g g a c g a g c g g c c a

8100 VSTQAFCSMKRKDISQVLRS DAGLGKAAHLPLIDALDQPA g t c a g c a c c c a g g c c t t t t g c a g c a t g a a g c g g a a g g a t a t c a g c c a g g t c c t g c g g a g c c a g t c g t g g g t c c g g a a a a c g t c g t a c t t c g c c t t c c t a t a g t c g g t c c a g g a c g c c t c g

8160 AVSQYVSDLSPAQQQGILSK GHALVHGVQGRGLLLADQAL g c c g t g a g c c a g t a c g t g t c c g a c c t g t c c c c t g c c c a g c a g c a g g g c a t c c t g a g c a a g c g g c a c t c g g t c a t g c a c a g g c t g g a c a g g g g a c g g g t c g t c g t c c c g t a g g a c t c g t t c

8220 MVQAEDTAPGIVEIQGAFFK HHLGLICGWADHLNLAGEEF *PAKKL a t g g t g c a g g c c g a g g a t a c a g c c c c a g g c a t c g t g g a g a t t c a g g g c g c c t t c t t c a a a t a c c a c g t c c g g c t c c t a t g t c g g g g t c c g t a g c a c c t c t a a g t c c c g c g g a a g a a g t t t

8280 EVSLFDLRRQPGFNSTVLKD LHGQEVQSPLGAEVAGHQLV STDRNSRLLCGPKLLVTSFS g a g g t g t c c c t g t t c g a c c t g a g a a g g c a g c c c g g c t t c a a c a g c a c c g t g c t g a a g g a c c t c c a c a g g g a c a a g c t g g a c t c t t c c g t c g g g c c g a a g t t g t c g t g g c a c g a c t t c c t g

8340 KELGRSQALFLYELLLKTTR FLQASALCQEQVLQQQFGGS LSSPLLWASNRYSSSSFVVL a a a g a g c t g g g c a g a a g c c a g g c a c t g t t c c t g t a c g a g c t g c t g c t g a a a a c c a c c a g a t t t c t c g a c c c g t c t t c g g t c c g t g a c a a g g a c a t g c t c g a c g a c g a c t t t t g g t g g t c t

8400 RPEELLSAGQLVKGVTCSHI PGLFQQRGALQDLAHGTAVD RGSSSSDAPWSTLPTVQLWM c g g c c c g a g g a a c t g c t g t c t g c c g g c c a g c t g g t c a a g g g c g t g a c c t g t a g c c a c a t c g c c g g g c t c c t t g a c g a c a g a c g g c c g g t c g a c c a g t t c c c g c a c t g g a c a t c g g t g t a g

8460 DAMSTDFFLAHFQDFQNNFA VGHAGVKKQGVELVELVVEG SAMLVSKKRAWKWSKWFLKA g a c g c c a t g a g c a c c g a c t t t t t t c t g g c c c a c t t c c a g g a c t t c c a g a a c a a c t t c g c c c t g c g g t a c t c g t g g c t g a a a a a a g a c c g g g t g a a g g t c c t g a a g g t c t t g t t g a a g c g g

8520 LLSPYQVNCLAWKYWEVSRL QQGWVLDVAQGPLVPLHGSQ RSDGYWTLQRAQFYQSTDLS c t g c t g t c c c c a t a c c a g g t c a a c t g c c t g g c c t g g a a g t a c t g g g a g g t g t c c a g a c t g g a c g a c a g g g g t a t g g t c c a g t t g a c g g a c c g g a c c t t c a t g a c c c t c c a c a g g t c t g a c

NheISphI 8580 SMPPFLLAALPARYLASVPA AHGGKQEGGQRGSVQGAHGS LMGGKRSAARGALYRALTGA a g c a t g c c c c c c t t t c t g c t c g c c g c c c t g c c t g c c a g a t a c c t g g c c a g c g t g c c c g c t t c g t a c g g g g g g a a a g a c g a g c g g c g g g a c g g a c g g t c t a t g g a c c g g t c g c a c g g g c g a

BmtI 8640 SQCVPFLISLGKSWLDSLVL ALAHGKQDAQALAPQVAQHQ LWHTGKRILRPLLQSSLRTS a g c c a g t g c g t g c c c t t t c t g a t c a g c c t g g g c a a g a g c t g g c t g g a c a g c c t g g t g c t g t c g g t c a c g c a c g g g a a a g a c t a g t c g g a c c c g t t c t c g a c c g a c c t g t c g g a c c a c g a c

8700 DSHKKTSVLRKVQQCLDDSI VGVLFGAHQPLHLLAQVVAN SEWLFVLTSRFTCCHRSSLM g a c t c c c a c a a g a a a a c c a g c g t g c t g c g g a a g g t g c a g c a g t g c c t g g a c g a c a g c a t t c t g a g g g t g t t c t t t t g g t c g c a c g a c g c c t t c c a c g t c g t c a c g g a c c t g c t g t c g t a a

8760 ADEYTVDIMGNLLCHLPAAI GVLVGHVDHAIQQAMQRGGD ASSYVTSMMPLRSHWRGAAM g c c g a c g a g t a c a c c g t g g a c a t c a t g g g c a a t c t g c t g t g c c a t c t g c c t g c c g c c a t c c g g c t g c t c a t g t g g c a c c t g t a g t a c c c g t t a g a c g a c a c g g t a g a c g g a c g g c g g t a g

8820 IDRGISPRAWATALHGLRDC DVSADARSGPGCGEMAQSVT MSLPM a t c g a c a g a g g c a t c a g c c c t a g a g c c t g g g c c a c a g c c c t c c a t g g c c t g a g a g a c t g t t a g c t g t c t c c g t a g t c g g g a t c t c g g a c c c g g t g t c g g g a g g t a c c g g a c t c t c t g a c a

SfiI 8880 PDLNPEQKAAVRLKLLGQYG GVQVRLLFGGHPQFQQALVA c c c g a c c t g a a c c c t g a g c a g a a a g c c g c c g t g c g g c t g a a a c t g c t g g g c c a g t a c g g c g g g c t g g a c t t g g g a c t c g t c t t t c g g c g g c a c g c c g a c t t t g a c g a c c c g g t c a t g c c g

8940 LPQHWTAETTKDLGPFLVLF QRLVPGGLCGLVQAGEQHQE c t g c c t c a g c a c t g g a c c g c c g a g a c a a c c a a g g a c c t g g g c c c c t t c c t g g t g c t g t t c g a c g g a g t c g t g a c c t g g c g g c t c t g t t g g t t c c t g g a c c c g g g g a a g g a c c a c g a c a a g

9000 SGDELSSIATKFPEILLQAA AAVLQGANGGLEGLDEQLSG a g c g g c g a c g a g c t g t c c a g c a t t g c c a c c a a g t t c c c c g a g a t c c t c c t g c a g g c t g c c t c g c c g c t g c t c g a c a g g t c g t a a c g g t g g t t c a a g g g g c t c t a g g a g g a c g t c c g a c g g

9060 SKMARTLPTKEFLWAVFQSV ALHGPGQGGFFKQPGHELAH a g c a a g a t g g c c c g g a c c c t g c c c a c c a a a g a a t t t c t g t g g g c c g t g t t c c a g a g c g t g t c g t t c t a c c g g g c c t g g g a c g g g t g g t t t c t t a a a g a c a c c c g g c a c a a g g t c t c g c a c

9120 RNSSDKIPSYDPMPGCHGVV PVAAVLDGAVVGHRATM c g g a a c a g c a g c g a c a a g a t c c c c a g c t a c g a c c c c a t g c c t g g c t g t c a t g g c g t g g t g g c c t t g t c g t c g c t g t t c t a g g g g t c g a t g c t g g g g t a c g g a c c g a c a g t a c c g c a c c a c

AbsI 9180 APSSDDIFKLAEANACWALE g c c c c c a g c a g c g a c g a c a t c t t c a a g c t g g c t g a g g c c a a t g c c t g c t g g g c c c t c g a g c g g g g g t c g t c g c t g c t g t a g a a g t t c g a c c g a c t c c g g t t a c g g a c g a c c c g g g a g c t c

NsiI 9240 DLRCMEEDTFIRTVELLGAV g a c c t g a g a t g c a t g g a a g a g g a c a c c t t c a t c c g g a c c g t c g a a c t c c t c g g t g c a g t g c t g g a c t c t a c g t a c c t t c t c c t g t g g a a g t a g g c c t g g c a g c t t g a g g a g c c a c g t c a c

9300 QGFSRPQLMTLKEKAIQVWD c a g g g t t t t a g c a g a c c c c a g c t g a t g a c c c t g a a a g a a a a g g c c a t c c a g g t c t g g g a c g t c c c a a a a t c g t c t g g g g t c g a c t a c t g g g a c t t t c t t t t c c g g t a g g t c c a g a c c c t g

9360 MPSYWREHHIVSLGRIALAL a t g c c t a g c t a c t g g c g g g a g c a c c a c a t c g t g t c c c t g g g c a g a a t c g c c c t g g c c c t g t a c g g a t c g a t g a c c g c c c t c g t g g t g t a g c a c a g g g a c c c g t c t t a g c g g g a c c g g g a c

9420 NESELEQLDLSSIDTVASLS a a t g a a t c t g a g c t g g a a c a g c t g g a c c t g t c c t c c a t c g a c a c c g t g g c c a g c c t g t c c t t a c t t a g a c t c g a c c t t g t c g a c c t g g a c a g g a g g t a g c t g t g g c a c c g g t c g g a c a g g

9480 WQTEWTPGQAESILQGYLDD t g g c a g a c c g a g t g g a c a c c t g g c c a g g c c g a g t c c a t c c t g c a g g g c t a c c t g g a c g a c a c c g t c t g g c t c a c c t g t g g a c c g g t c c g g c t c a g g t a g g a c g t c c c g a t g g a c c t g c t g

9540 SGYSIQDLKSFHLVGLGATL t c c g g c t a c a g c a t c c a g g a c c t g a a g t c c t t c c a c c t c g t g g g a c t g g g a g c c a c a c t g a g g c c g a t g t c g t a g g t c c t g g a c t t c a g g a a g g t g g a g c a c c c t g a c c c t c g g t g t g a c

9600 CAINITEIPLIKISEFRVVV t g c g c c a t c a a t a t c a c c g a g a t c c c c c t g a t c a a g a t c a g c g a g t t c c g g g t g g t g g t g a c g c g g t a g t t a t a g t g g c t c t a g g g g g a c t a g t t c t a g t c g c t c a a g g c c c a c c a c c a c

9660 ARIGTLLCSTHVLAEFKRKA g c c a g a a t c g g c a c c c t g c t g t g c t c t a c c c a c g t g c t g g c c g a g t t c a a g a g a a a g g c c c g g t c t t a g c c g t g g g a c g a c a c g a g a t g g g t g c a c g a c c g g c t c a a g t t c t c t t t c c g g

9720 EVVFGDPTEWTSSVLQELGT g a g g t g g t g t t c g g c g a c c c c a c a g a g t g g a c c a g c t c c g t g c t g c a g g a a c t g g g c a c c c t c c a c c a c a a g c c g c t g g g g t g t c t c a c c t g g t c g a g g c a c g a c g t c c t t g a c c c g t g g

9780 IAAGLTKAELRMLDKDLMPY a t t g c c g c c g g a c t g a c c a a g g c c g a a c t g c g g a t g c t g g a c a a g g a c c t g a t g c c c t a c t a a c g g c g g c c t g a c t g g t t c c g g c t t g a c g c c t a c g a c c t g t t c c t g g a c t a c g g g a t g

9840 FQPSAIKCLPDEIFKELSAE t t c c a g c c c a g c g c c a t c a a g t g t c t g c c c g a c g a g a t c t t t a a a g a g c t g t c c g c c g a a a a g g t c g g g t c g c g g t a g t t c a c a g a c g g g c t g c t c t a g a a a t t t c t c g a c a g g c g g c t t

9900 QIASLGPENAAAVTHAQRRR c a g a t c g c c a g c c t g g g a c c a g a a a a t g c c g c c g c t g t g a c c c a c g c c c a g a g a a g a a g g g t c t a g c g g t c g g a c c c t g g t c t t t t a c g g c g g c g a c a c t g g g t g c g g g t c t c t t c t t c c

9960 LSPLQLQSLQQALDGAKTHS c t g t c c c c c c t g c a g c t c c a g t c t c t g c a g c a g g c c c t g g a t g g c g c c a a g a c c c a c t c t g a c a g g g g g g a c g t c g a g g t c a g a g a c g t c g t c c g g g a c c t a c c g c g g t t c t g g g t g a g a

BssHIIAscI 10,020 WQDAPASAGPTRTSSSRSPG t g g c a g g a t g c c c c a g c c a g c g c c g g a c c t a c c a g a a c c a g c a g c t c c a g a t c t c c t g g c a c c g t c c t a c g g g g t c g g t c g c g g c c t g g a t g g t c t t g g t c g t c g a g g t c t a g a g g a c c g

10,025 AP g c g c c c g c g g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.