Addgene SpyTag-β-Lactamase-SpyCatcher - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: SpyTag-β-Lactamase-SpyCatcher


Map View

1250 1000 750 500 250 End (1284) BciVI (1154) PfoI * (1146) ApoI (1021) MfeI (992) BtgI - StyI - NcoI - PluTI - HaeII (935) SfoI (933) NarI (932) KasI (931) AhdI (842) BmrI (802) BsaI (776) BpmI (773) BsrFI (757) BglI (724) EciI (700) NmeAIII (695) AseI (667) FspI (619) PstI (600) SfcI (596) PvuI (473) EaeI (449) TsoI (444) ScaI (361) Amp-R (273 .. 292) DraI (264) BpuEI (238) SmlI (217) AcuI (176) BaeGI - Bme1580I * (174) BssSI - BssSαI (173) ApaLI (170) BstEII (121) DraIII (75) BanII (69) NdeI (59) Start (0) 6xHis thrombin site bla(M) SpyTag- β- Lactamase-SpyCatcher 1284 bp

Sequence View

NdeIStart (0) 60 1 5 HHHHHH SSG 1 5 LVPRGS H 6xHis thrombin site MGSSHHHHHHSSGLVPRGSH a t g g g c a g c a g c c a t c a t c a t c a t c a t c a c a g c a g c g g c c t g g t g c c g c g c g g c a g c c a t t a c c c g t c g t c g g t a g t a g t a g t a g t a g t g t c g t c g c c g g a c c a c g g c g c g c c g t c g g t a

DraIIIBanII 120 (in frame with thrombin site) MGAHIVMVDAYKPTKGSGGS MGAHIVMVDAYKPTKGSGGS a t g g g a g c c c a c a t c g t g a t g g t g g a c g c c t a c a a g c c g a c g a a g g g t t c a g g g g g t t c c t a c c c t c g g g t g t a g c a c t a c c a c c t g c g g a t g t t c g g c t g c t t c c c a a g t c c c c c a a g g

ApaLIBssSαIBssSIBme1580I*BaeGIAcuIBstEII 180 GH 5 10 15 20 PETLVKVKDAEDQLGARV bla(M) GHPETLVKVKDAEDQLGARV g g t c a c c c a g a a a c g c t g g t g a a a g t a a a a g a t g c t g a a g a t c a g t t g g g t g c a c g a g t g c c a g t g g g t c t t t g c g a c c a c t t t c a t t t t c t a c g a c t t c t a g t c a a c c c a c g t g c t c a c

BpuEISmlI 240 25 30 35 40 GYIELDLNSGKILESFRPEE bla(M) GYIELDLNSGKILESFRPEE g g t t a c a t c g a a c t g g a t c t c a a c a g c g g t a a g a t c c t t g a g a g t t t t c g c c c c g a a g a a c c a a t g t a g c t t g a c c t a g a g t t g t c g c c a t t c t a g g a a c t c t c a a a a g c g g g g c t t c t t

DraI 300 45 50 55 60 RFPMMSTFKVLLCGAVLSRI bla(M) C G A T A C A C C G C G C C A T A A T A Amp-R RFPMMSTFKVLLCGAVLSRI c g t t t t c c a a t g a t g a g c a c t t t t a a a g t t c t g c t a t g t g g c g c g g t a t t a t c c c g t a t t g c a a a a g g t t a c t a c t c g t g a a a a t t t c a a g a c g a t a c a c c g c g c c a t a a t a g g g c a t a a

360 65 70 75 80 DAGQEQLGRRIHYSQNDLVE bla(M) DAGQEQLGRRIHYSQNDLVE g a c g c c g g g c a a g a g c a a c t c g g t c g c c g c a t a c a c t a t t c t c a g a a t g a c t t g g t t g a g c t g c g g c c c g t t c t c g t t g a g c c a g c g g c g t a t g t g a t a a g a g t c t t a c t g a a c c a a c t c

ScaI 420 85 90 95 100 YSPVTEKHLTDGMTVRELCS bla(M) YSPVTEKHLTDGMTVRELCS t a c t c a c c a g t c a c a g a a a a g c a t c t t a c g g a t g g c a t g a c a g t a a g a g a a t t a t g c a g t a t g a g t g g t c a g t g t c t t t t c g t a g a a t g c c t a c c g t a c t g t c a t t c t c t t a a t a c g t c a

PvuIEaeITsoI 480 105 110 115 120 AAITMSDNTAANLLLTTIGG bla(M) AAITMSDNTAANLLLTTIGG g c t g c c a t a a c c a t g a g t g a t a a c a c t g c g g c c a a c t t a c t t c t g a c a a c g a t c g g a g g a c g a c g g t a t t g g t a c t c a c t a t t g t g a c g c c g g t t g a a t g a a g a c t g t t g c t a g c c t c c t

540 125 130 135 140 PKELTAFLHNMGDHVTRLDR bla(M) PKELTAFLHNMGDHVTRLDR c c g a a g g a g c t a a c c g c t t t t t t g c a c a a c a t g g g g g a t c a t g t a a c t c g c c t t g a t c g t g g c t t c c t c g a t t g g c g a a a a a a c g t g t t g t a c c c c c t a g t a c a t t g a g c g g a a c t a g c a

PstISfcI 600 145 150 155 160 WEPELNEAIPNDERDTTMPA bla(M) WEPELNEAIPNDERDTTMPA t g g g a a c c g g a g c t g a a t g a a g c c a t a c c a a a c g a c g a g c g t g a c a c c a c g a t g c c t g c a a c c c t t g g c c t c g a c t t a c t t c g g t a t g g t t t g c t g c t c g c a c t g t g g t g c t a c g g a c g t

FspI 660 165 170 175 180 AMATTLRKLLTGELLTLASR bla(M) AMATTLRKLLTGELLTLASR g c a a t g g c a a c a a c g t t g c g c a a a c t a t t a a c t g g c g a a c t a c t t a c t c t a g c t t c c c g g c g t t a c c g t t g t t g c a a c g c g t t t g a t a a t t g a c c g c t t g a t g a a t g a g a t c g a a g g g c c

NmeAIIIEciIAseI 720 185 190 195 200 QQLIDWMEADKVAGPLLRSA bla(M) QQLIDWMEADKVAGPLLRSA c a a c a a t t a a t a g a c t g g a t g g a g g c g g a t a a a g t t g c a g g a c c a c t t c t g c g c t c g g c c g t t g t t a a t t a t c t g a c c t a c c t c c g c c t a t t t c a a c g t c c t g g t g a a g a c g c g a g c c g g

BpmIBsaIBsrFIBglI 780 205 210 215 220 LPAGWFIADKSGAGERGSRG bla(M) LPAGWFIADKSGAGERGSRG c t t c c g g c t g g c t g g t t t a t t g c t g a t a a a t c t g g a g c c g g t g a g c g t g g g t c t c g c g g t g a a g g c c g a c c g a c c a a a t a a c g a c t a t t t a g a c c t c g g c c a c t c g c a c c c a g a g c g c c a

BmrI 840 225 230 235 240 IIAALGPDGKPSRIVVIYTT bla(M) IIAALGPDGKPSRIVVIYTT a t c a t t g c a g c a c t g g g g c c a g a t g g t a a g c c c t c c c g t a t c g t a g t t a t c t a c a c g a c g t a g t a a c g t c g t g a c c c c g g t c t a c c a t t c g g g a g g g c a t a g c a t c a a t a g a t g t g c t g c

AhdI 900 245 250 255 260 GSQATMDERNRQIAEIGASL bla(M) GSQATMDERNRQIAEIGASL g g g a g t c a g g c a a c t a t g g a t g a a c g a a a t a g a c a g a t c g c t g a g a t a g g t g c c t c a c t g c c c t c a g t c c g t t g a t a c c t a c t t g c t t t a t c t g t c t a g c g a c t c t a t c c a c g g a g t g a c

KasINarISfoIHaeIIPluTINcoIStyIBtgI 960 IKHW (in frame with bla(M)) GSGGSGGAMVDTLSGL bla(M) IKHWGSGGSGGAMVDTLSGL a t t a a g c a t t g g g g g a g t g g t g g c a g c g g a g g c g c c a t g g t t g a t a c c t t a t c a g g t t t a t a a t t c g t a a c c c c c t c a c c a c c g t c g c c t c c g c g g t a c c a a c t a t g g a a t a g t c c a a a t

MfeI 1020 (in frame with bla(M)) SSEQGQSGDMTIEEDSATHI SSEQGQSGDMTIEEDSATHI t c a a g t g a g c a a g g t c a g t c c g g t g a t a t g a c a a t t g a a g a a g a t a g t g c t a c c c a t a t t a g t t c a c t c g t t c c a g t c a g g c c a c t a t a c t g t t a a c t t c t t c t a t c a c g a t g g g t a t a a

ApoI 1080 (in frame with bla(M)) KFSKRDEDGKELAGATMELR KFSKRDEDGKELAGATMELR a a a t t c t c a a a a c g t g a t g a g g a c g g c a a a g a g t t a g c t g g t g c a a c t a t g g a g t t g c g t t t t a a g a g t t t t g c a c t a c t c c t g c c g t t t c t c a a t c g a c c a c g t t g a t a c c t c a a c g c a

1140 (in frame with bla(M)) DSSGKTISTWISDGQVKDFY DSSGKTISTWISDGQVKDFY g a t t c a t c t g g t a a a a c t a t t a g t a c a t g g a t t t c a g a t g g a c a a g t g a a a g a t t t c t a c c t a a g t a g a c c a t t t t g a t a a t c a t g t a c c t a a a g t c t a c c t g t t c a c t t t c t a a a g a t g

BciVIPfoI* 1200 (in frame with bla(M)) LYPGKYTFVETAAPDGYEVA LYPGKYTFVETAAPDGYEVA c t g t a t c c a g g a a a a t a t a c a t t t g t c g a a a c c g c a g c a c c a g a c g g t t a t g a g g t a g c a g a c a t a g g t c c t t t t a t a t g t a a a c a g c t t t g g c g t c g t g g t c t g c c a a t a c t c c a t c g t

1260 (in frame with bla(M)) TAITFTVNEQGQVTVNGKAT TAITFTVNEQGQVTVNGKAT a c t g c t a t t a c c t t t a c a g t t a a t g a g c a a g g t c a g g t t a c t g t a a a t g g c a a a g c a a c t t g a c g a t a a t g g a a a t g t c a a t t a c t c g t t c c a g t c c a a t g a c a t t t a c c g t t t c g t t g a

End (1284) 1284 (in frame with bla(M)) KGDAHI* KGDAHI* a a a g g t g a c g c t c a t a t t t a a t g a t t t c c a c t g c g a g t a t a a a t t a c t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.