Addgene pAG414GAL-Cerulean-ccdB - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pAG414GAL-Cerulean-ccdB


Map View

pGEX 3' (7839 .. 7861) pRS-marker (7720 .. 7739) SnaBI (7570) BstAPI (7346) MfeI (7278) Bsu36I (7177) EcoRV (7137) PaqCI (6985) HindIII (6905) F1ori-F (6491 .. 6512) DraIII (6427) NaeI (6321) NgoMIV (6319) F1ori-R (6281 .. 6300) M13/pUC Forward (6042 .. 6064) M13 Forward (6033 .. 6050) M13 fwd T7 (6004 .. 6023) T7 promoter KpnI (5995) Acc65I (5991) MluI (5911) CYC1 (5755 .. 5773) SalI (5602) PstI (5600) ccdB-fwd (5446 .. 5465) BmgBI (5379) BsaBI * (5358) SrfI (5345) BbvCI (5199) BtgI - NcoI - StyI (4772) PasI (4703) EcoRI (4471) CAT-R (4324 .. 4343) NotI (4151) CsiI - SexAI * (4006) EGFP-C (3937 .. 3958) pBRforEco (10 .. 28) SwaI (544) Amp-R (933 .. 952) AhdI (1502) pBR322ori-F (2235 .. 2254) PciI (2390) L4440 (2488 .. 2505) M13/pUC Reverse (2701 .. 2723) lac operator M13 Reverse (2720 .. 2736) M13 rev T3 (2755 .. 2775) Eco53kI (2790) SacI (2792) AgeI (2868) GAL1 (3211 .. 3234) pBluescriptSK (3255 .. 3271) SpeI (3261) SK primer EGFP-N (3329 .. 3350) EXFP-R (3590 .. 3609) PflFI - Tth111I (3731) BlpI (3905) pAG414GAL-Cerulean-ccdB 7889 bp

Sequence View

5ʹ 3ʹ 60 C G G A G C A C T A T G C G G A T A A pBRforEco g a c g a a a g g g c c t c g t g a t a c g c c t a t t t t t a t a g g t t a a t g t c a t g a t a a t a a t g g t t t c t g c t t t c c c g g a g c a c t a t g c g g a t a a a a a t a t c c a a t t a c a g t a c t a t t a t t a c c a a a

120 CEN/ARS c t t a g g a c g g a t c g c t t g c c t g t a a c t t a c a c g c g c c t c g t a t c t t t t a a t g a t g g a a t a g a a t c c t g c c t a g c g a a c g g a c a t t g a a t g t g c g c g g a g c a t a g a a a a t t a c t a c c t t a t

180 CEN/ARS a t t t g g g a a t t t a c t c t g t g t t t a t t t a t t t t t a t g t t t t g t a t t t g g a t t t t a g a a a g t t a a a c c c t t a a a t g a g a c a c a a a t a a a t a a a a a t a c a a a a c a t a a a c c t a a a a t c t t t c a

240 CEN/ARS a a a t a a a g a a g g t a g a a g a g t t a c g g a a t g a a g a a a a a a a a a t a a a c a a a g g t t t a a a a a t t t a t t t c t t c c a t c t t c t c a a t g c c t t a c t t c t t t t t t t t t a t t t g t t t c c a a a t t t t t

300 CEN/ARS a t t t c a a c a a a a a g c g t a c t t t a c a t a t a t a t t t a t t a g a c a a g a a a a g c a g a t t a a a t a t a a a g t t g t t t t t c g c a t g a a a t g t a t a t a t a a a t a a t c t g t t c t t t t c g t c t a a t t t a t

360 CEN/ARS g a t a t a c a t t c g a t t a a c g a t a a g t a a a a t g t a a a a t c a c a g g a t t t t c g t g t g t g g t c t c t a t a t g t a a g c t a a t t g c t a t t c a t t t t a c a t t t t a g t g t c c t a a a a g c a c a c a c c a g a

420 CEN/ARS t c t a c a c a g a c a a g a t g a a a c a a t t c g g c a t t a a t a c c t g a g a g c a g g a a g a g c a a g a t a a g a t g t g t c t g t t c t a c t t t g t t a a g c c g t a a t t a t g g a c t c t c g t c c t t c t c g t t c t a t

480 CEN/ARS a a a g g t a g t a t t t g t t g g c g a t c c c c c t a g a g t c t t t t a c a t c t t c g g a a a a c a a a a a c t t t t c c a t c a t a a a c a a c c g c t a g g g g g a t c t c a g a a a a t g t a g a a g c c t t t t g t t t t t g a

540 CEN/ARS a t t t t t t c t t t a a t t t c t t t t t t t a c t t t c t a t t t t t a a t t t a t a t a t t t a t a t t a a a a a t a a a a a a g a a a t t a a a g a a a a a a a t g a a a g a t a a a a a t t a a a t a t a t a a a t a t a a t t t t t

SwaI 600 CEN/ARS a t t t a a a t t a t a a t t a t t t t t a t a g c a c g t g a t g a a a a g g a c c c a g g t g g c a c t t t t c g g t a a a t t t a a t a t t a a t a a a a a t a t c g t g c a c t a c t t t t c c t g g g t c c a c c g t g a a a a g c c

660 AmpR promoter g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c

720 1 signal se... MS AmpR promoter AmpR MS c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t a t g a g t g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a t a c t c a

780 5 10 15 20 signal sequence IQHFRVALIPFFAAFCLPVF AmpR IQHFRVALIPFFAAFCLPVF a t t c a a c a t t t c c g t g t c g c c c t t a t t c c c t t t t t t g c g g c a t t t t g c c t t c c t g t t t t t t a a g t t g t a a a g g c a c a g c g g g a a t a a g g g a a a a a a c g c c g t a a a a c g g a a g g a c a a a a a

840 sig... A 25 30 35 40 HPETLVKVKDAEDQLGARV AmpR AHPETLVKVKDAEDQLGARV g c t c a c c c a g a a a c g c t g g t g a a a g t a a a a g a t g c t g a a g a t c a g t t g g g t g c a c g a g t g c g a g t g g g t c t t t g c g a c c a c t t t c a t t t t c t a c g a c t t c t a g t c a a c c c a c g t g c t c a c

900 45 50 55 60 GYIELDLNSGKILESFRPEE AmpR GYIELDLNSGKILESFRPEE g g t t a c a t c g a a c t g g a t c t c a a c a g c g g t a a g a t c c t t g a g a g t t t t c g c c c c g a a g a a c c a a t g t a g c t t g a c c t a g a g t t g t c g c c a t t c t a g g a a c t c t c a a a a g c g g g g c t t c t t

960 65 70 75 80 RFPMMSTFKVLLCGAVLSRI AmpR C G A T A C A C C G C G C C A T A A T A Amp-R RFPMMSTFKVLLCGAVLSRI c g t t t t c c a a t g a t g a g c a c t t t t a a a g t t c t g c t a t g t g g c g c g g t a t t a t c c c g t a t t g c a a a a g g t t a c t a c t c g t g a a a a t t t c a a g a c g a t a c a c c g c g c c a t a a t a g g g c a t a a

1020 85 90 95 100 DAGQEQLGRRIHYSQNDLVE AmpR DAGQEQLGRRIHYSQNDLVE g a c g c c g g g c a a g a g c a a c t c g g t c g c c g c a t a c a c t a t t c t c a g a a t g a c t t g g t t g a g c t g c g g c c c g t t c t c g t t g a g c c a g c g g c g t a t g t g a t a a g a g t c t t a c t g a a c c a a c t c

1080 105 110 115 120 YSPVTEKHLTDGMTVRELCS AmpR YSPVTEKHLTDGMTVRELCS t a c t c a c c a g t c a c a g a a a a g c a t c t t a c g g a t g g c a t g a c a g t a a g a g a a t t a t g c a g t a t g a g t g g t c a g t g t c t t t t c g t a g a a t g c c t a c c g t a c t g t c a t t c t c t t a a t a c g t c a

1140 125 130 135 140 AAITMSDNTAANLLLTTIGG AmpR AAITMSDNTAANLLLTTIGG g c t g c c a t a a c c a t g a g t g a t a a c a c t g c g g c c a a c t t a c t t c t g a c a a c g a t c g g a g g a c g a c g g t a t t g g t a c t c a c t a t t g t g a c g c c g g t t g a a t g a a g a c t g t t g c t a g c c t c c t

1200 145 150 155 160 PKELTAFLHNMGDHVTRLDR AmpR PKELTAFLHNMGDHVTRLDR c c g a a g g a g c t a a c c g c t t t t t t g c a c a a c a t g g g g g a t c a t g t a a c t c g c c t t g a t c g t g g c t t c c t c g a t t g g c g a a a a a a c g t g t t g t a c c c c c t a g t a c a t t g a g c g g a a c t a g c a

1260 165 170 175 180 WEPELNEAIPNDERDTTMPV AmpR WEPELNEAIPNDERDTTMPV t g g g a a c c g g a g c t g a a t g a a g c c a t a c c a a a c g a c g a g c g t g a c a c c a c g a t g c c t g t a a c c c t t g g c c t c g a c t t a c t t c g g t a t g g t t t g c t g c t c g c a c t g t g g t g c t a c g g a c a t

1320 185 190 195 200 AMATTLRKLLTGELLTLASR AmpR AMATTLRKLLTGELLTLASR g c a a t g g c a a c a a c g t t g c g c a a a c t a t t a a c t g g c g a a c t a c t t a c t c t a g c t t c c c g g c g t t a c c g t t g t t g c a a c g c g t t t g a t a a t t g a c c g c t t g a t g a a t g a g a t c g a a g g g c c

1380 205 210 215 220 QQLIDWMEADKVAGPLLRSA AmpR QQLIDWMEADKVAGPLLRSA c a a c a a t t a a t a g a c t g g a t g g a g g c g g a t a a a g t t g c a g g a c c a c t t c t g c g c t c g g c c g t t g t t a a t t a t c t g a c c t a c c t c c g c c t a t t t c a a c g t c c t g g t g a a g a c g c g a g c c g g

1440 225 230 235 240 LPAGWFIADKSGAGERGSRG AmpR LPAGWFIADKSGAGERGSRG c t t c c g g c t g g c t g g t t t a t t g c t g a t a a a t c t g g a g c c g g t g a g c g t g g g t c t c g c g g t g a a g g c c g a c c g a c c a a a t a a c g a c t a t t t a g a c c t c g g c c a c t c g c a c c c a g a g c g c c a

1500 245 250 255 260 IIAALGPDGKPSRIVVIYTT AmpR IIAALGPDGKPSRIVVIYTT a t c a t t g c a g c a c t g g g g c c a g a t g g t a a g c c c t c c c g t a t c g t a g t t a t c t a c a c g a c g t a g t a a c g t c g t g a c c c c g g t c t a c c a t t c g g g a g g g c a t a g c a t c a a t a g a t g t g c t g c

AhdI 1560 265 270 275 280 GSQATMDERNRQIAEIGASL AmpR GSQATMDERNRQIAEIGASL g g g a g t c a g g c a a c t a t g g a t g a a c g a a a t a g a c a g a t c g c t g a g a t a g g t g c c t c a c t g c c c t c a g t c c g t t g a t a c c t a c t t g c t t t a t c t g t c t a g c g a c t c t a t c c a c g g a g t g a c

1620 285 IKHW* AmpR IKHW* a t t a a g c a t t g g t a a c t g t c a g a c c a a g t t t a c t c a t a t a t a c t t t a g a t t g a t t t a a a a t a a t t c g t a a c c a t t g a c a g t c t g g t t c a a a t g a g t a t a t a t g a a a t c t a a c t a a a t t t t

1680 c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a a g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t t

1740 a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t

1800 ori t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c

1860 ori c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a

1920 ori g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t c c t t c t a g t g t a g c c g t a g t t a g g c c a c c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a g g a a g a t c a c a t c g g c a t c a a t c c g g t g

1980 ori c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c

2040 ori g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c

2100 ori g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t

2160 ori a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g

2220 ori g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c

2280 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g

2340 ori t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g

PciI 2400 a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a

2460 c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c t t t g a g t g a g c t g a t a c c g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c g a c t a t g g

2520 A G C G A G T C A G T G A G C G A G L4440 g c t c g c c g c a g c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g c g a g g a a g c g g a a g a g c g c c g a g c g g c g t c g g c t t g c t g g c t c g c g t c g c t c a g t c a c t c g c t c c t t c g c c t t c t c g c g

2580 c c a a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g g c a c g a c g g t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c c g t g c t g

2640 a g g t t t c c c g a c t g g a a a g c g g g c a g t g a g c g c a a c g c a a t t a a t g t g a g t t a c c t c a c t t c c a a a g g g c t g a c c t t t c g c c c g t c a c t c g c g t t g c g t t a a t t a c a c t c a a t g g a g t g a

2700 -35 -10 lac promoter lac operator c a t t a g g c a c c c c a g g c t t t a c a c t t t a t g c t t c c g g c t c c t a t g t t g t g t g g a a t t g t g g t a a t c c g t g g g g t c c g a a a t g t g a a a t a c g a a g g c c g a g g a t a c a a c a c a c c t t a a c a c

2760 lac operator M13 rev T3 promoter A G C G G A T A A C A A T T T C A C A C A G G M13/pUC Reverse C A G G A A A C A G C T A T G A C M13 Reverse G C A A T T T3 a g c g g a t a a c a a t t t c a c a c a g g a a a c a g c t a t g a c c a t g a t t a c g c c a a g c g c g c a a t t t c g c c t a t t g t t a a a g t g t g t c c t t t g t c g a t a c t g g t a c t a a t g c g g t t c g c g c g t t a a

Eco53kISacI 2820 T3 promoter GAL1 promoter UAS A A C C C T C A C T A A A G G T3 a a c c c t c a c t a a a g g g a a c a a a a g c t g g a g c t c t a g t a c g g a t t a g a a g c c g c c g a g c g g t t g g g a g t g a t t t c c c t t g t t t t c g a c c t c g a g a t c a t g c c t a a t c t t c g g c g g c t c g c c

AgeI 2880 GAL1 promoter UAS g c g a c a g c c c t c c g a c g g a a g a c t c t c c t c c g t g c g t c c t c g t c t t c a c c g g t c g c g t t c c g c t g t c g g g a g g c t g c c t t c t g a g a g g a g g c a c g c a g g a g c a g a a g t g g c c a g c g c a a g

2940 GAL1 promoter UAS c t g a a a c g c a g a t g t g c c t c g c g c c g c a c t g c t c c g a a c a a t a a a g a t t c t a c a a t a c t a g a c t t t g c g t c t a c a c g g a g c g c g g c g t g a c g a g g c t t g t t a t t t c t a a g a t g t t a t g a t

3000 GAL1 promoter g c t t t t a t g g t t a t g a a g a g g a a a a a t t g g c a g t a a c c t g g c c c c a c a a a c c t t c a a a t t c g a a a a t a c c a a t a c t t c t c c t t t t t a a c c g t c a t t g g a c c g g g g t g t t t g g a a g t t t a a

3060 GAL1 promoter a a c g a a t c a a a t t a a c a a c c a t a g g a t g a t a a t g c g a t t a g t t t t t t a g c c t t a t t t c t g t t g c t t a g t t t a a t t g t t g g t a t c c t a c t a t t a c g c t a a t c a a a a a a t c g g a a t a a a g a c

3120 GAL1 promoter g g g t a a t t a a t c a g c g a a g c g a t g a t t t t t g a t c t a t t a a c a g a t a t a t a a a t g g a a a a g c c c a t t a a t t a g t c g c t t c g c t a c t a a a a a c t a g a t a a t t g t c t a t a t a t t t a c c t t t t c

3180 GAL1 promoter c t g c a t a a c c a c t t t a a c t a a t a c t t t c a a c a t t t t c a g t t t g t a t t a c t t c t t a t t c a a g a c g t a t t g g t g a a a t t g a t t a t g a a a g t t g t a a a a g t c a a a c a t a a t g a a g a a t a a g t t

3240 GAL1 promoter A A T A T A C C T C T A T A C T T T A A C G T C GAL1 *PS a t g t c a t a a a a g t a t c a a c a a a a a a t t g t t a a t a t a c c t c t a t a c t t t a a c g t c a a g g a g t a c a g t a t t t t c a t a g t t g t t t t t t a a c a a t t a t a t g g a g a t a t g a a a t t g c a g t t c c t c

SpeI 3300 1 M 1a V 5 SKGE SK primer SCFP3A T C T A G A A C T A G T G G A T C pBluescriptSK MVSKGE FVGSELVLPDGPVFHHALAL a a a a a a c c c c g g a t t c t a g a a c t a g t g g a t c c c c c g g g a c a a a a t g g t g a g c a a g g g c g a t t t t t t g g g g c c t a a g a t c t t g a t c a c c t a g g g g g c c c t g t t t t a c c a c t c g t t c c c g c t

3360 10 15 20 25 ELFTGVVPILVELDGDVNGH SCFP3A G A C C A G C T C G A C C T G C C G C T G C EGFP-N ELFTGVVPILVELDGDVNGH LQEGPHHGDQDLQVAVYVAV g g a g c t g t t c a c c g g g g t g g t g c c c a t c c t g g t c g a g c t g g a c g g c g a c g t a a a c g g c c a c c t c g a c a a g t g g c c c c a c c a c g g g t a g g a c c a g c t c g a c c t g c c g c t g c a t t t g c c g g t

3420 30 35 40 45 KFSVSGEGEGDATYGKLTLK SCFP3A KFSVSGEGEGDATYGKLTLK LEAHGALALAIGGVALQGQL c a a g t t c a g c g t g t c c g g c g a g g g c g a g g g c g a t g c c a c c t a c g g c a a g c t g a c c c t g a a g t t c a a g t c g c a c a g g c c g c t c c c g c t c c c g c t a c g g t g g a t g c c g t t c g a c t g g g a c t t

3480 50 55 60 65 FICTTGKLPVPWPTLVTTLT SCFP3A FICTTGKLPVPWPTLVTTLT EDAGGALQGHGPGGEHGGQG g t t c a t c t g c a c c a c c g g c a a g c t g c c c g t g c c c t g g c c c a c c c t c g t g a c c a c c c t g a c c a a g t a g a c g t g g t g g c c g t t c g a c g g g c a c g g g a c c g g g t g g g a g c a c t g g t g g g a c t g

3540 70 75 80 85 WGVQCFARYPDHMKQHDFFK SCFP3A WGVQCFARYPDHMKQHDFFK PAHLAEGAVGVVHLLVVEEL c t g g g g c g t g c a g t g c t t c g c c c g c t a c c c c g a c c a c a t g a a g c a g c a c g a c t t c t t c a a g a c c c c g c a c g t c a c g a a g c g g g c g a t g g g g c t g g t g t a c t t c g t c g t g c t g a a g a a g t t

3600 90 95 100 105 SAMPEGYVQERTIFFKDDGN SCFP3A C T G C T G C C G T T EXFP-R SAMPEGYVQERTIFFKDDGN GGHGFAVDLLAGDEELVVAV g t c c g c c a t g c c c g a a g g c t a c g t c c a g g a g c g c a c c a t c t t c t t c a a g g a c g a c g g c a a c a g g c g g t a c g g g c t t c c g a t g c a g g t c c t c g c g t g g t a g a a g a a g t t c c t g c t g c c g t t

3660 110 115 120 125 YKTRAEVKFEGDTLVNRIEL SCFP3A G A T G T T C T G EXFP-R YKTRAEVKFEGDTLVNRIEL VLGAGLHLELAVGQHVADLQ c t a c a a g a c c c g c g c c g a g g t g a a g t t c g a g g g c g a c a c c c t g g t g a a c c g c a t c g a g c t g a t g t t c t g g g c g c g g c t c c a c t t c a a g c t c c c g c t g t g g g a c c a c t t g g c g t a g c t c g a

3720 130 135 140 145 KGIDFKEDGNILGHKLEYNY SCFP3A KGIDFKEDGNILGHKLEYNY LADVELLVAVDQPVLQLVVV g a a g g g c a t c g a c t t c a a g g a g g a c g g c a a c a t c c t g g g g c a c a a g c t g g a g t a c a a c t a c t t c c c g t a g c t g a a g t t c c t c c t g c c g t t g t a g g a c c c c g t g t t c g a c c t c a t g t t g a t

Tth111IPflFI 3780 150 155 160 165 ISDNVYITADKQKNGIKANF SCFP3A ISDNVYITADKQKNGIKANF DAVVDIDGGVLLLVADLGVE c a t c a g c g a c a a c g t c t a t a t c a c c g c c g a c a a g c a g a a g a a c g g c a t c a a g g c c a a c t t g t a g t c g c t g t t g c a g a t a t a g t g g c g g c t g t t c g t c t t c t t g c c g t a g t t c c g g t t g a a

3840 170 175 180 185 KIRHNIEDGSVQLADHYQQN SCFP3A KIRHNIEDGSVQLADHYQQN LDAVVDLVAAHLEGVVVLLV c a a g a t c c g c c a c a a c a t c g a g g a c g g c a g c g t g c a g c t c g c c g a c c a c t a c c a g c a g a a g t t c t a g g c g g t g t t g t a g c t c c t g c c g t c g c a c g t c g a g c g g c t g g t g a t g g t c g t c t t

3900 190 195 200 205 TPIGDGPVLLPDNHYLSTQS SCFP3A TPIGDGPVLLPDNHYLSTQS GGDAVAGHQQGVVVVQAGLG c a c c c c c a t c g g c g a c g g c c c c g t g c t g c t g c c c g a c a a c c a c t a c c t g a g c a c c c a g t c g t g g g g g t a g c c g c t g c c g g g g c a c g a c g a c g g g c t g t t g g t g a t g g a c t c g t g g g t c a g

BlpI 3960 210 215 220 225 KLSKDPNEKRDHMVLLEFVT SCFP3A C A T G G T C C T G C T G G A G T T C G T G EGFP-C KLSKDPNEKRDHMVLLEFVT LQAFVGVLLAIVHDQQLEHG c a a g c t g a g c a a a g a c c c c a a c g a g a a g c g c g a t c a c a t g g t c c t g c t g g a g t t c g t g a c g t t c g a c t c g t t t c t g g g g t t g c t c t t c g c g c t a g t g t a c c a g g a c g a c c t c a a g c a c t g

SexAI*CsiI 4020 230 235 AAGITLGMDELYK (in frame with SCFP3A) GGPGGGH SCFP3A AAGITLGMDELYKGGPGGGH GGPDSEAHVLQVLTSWTTSM c g c c g c c g g g a t c a c t c t c g g c a t g g a c g a g c t g t a c a a g g g t g g a c c a g g t g g t g g a c a g c g g c g g c c c t a g t g a g a g c c g t a c c t g c t c g a c a t g t t c c c a c c t g g t c c a c c a c c t g t

4080 (in frame with SCFP3A) QTSLYKKAERET* attR1 QTSLYKKAERET* t c a a a c a a g t t t g t a c a a a a a a g c t g a a c g a g a a a c g t a a a a t g a t a t a a a t a t c a a t a t a g t t t g t t c a a a c a t g t t t t t t c g a c t t g c t c t t t g c a t t t t a c t a t a t t t a t a g t t a t a

4140 attR1 a t t a a a t t a g a t t t t g c a t a a a a a a c a g a c t a c a t a a t a c t g t a a a a c a c a a c a t a t c c a t a a t t t a a t c t a a a a c g t a t t t t t t g t c t g a t g t a t t a t g a c a t t t t g t g t t g t a t a g g t

NotI 4200 -35 -10 attR1 lac UV5 promoter g t c a c t a t g g c g g c c g c a t t a g g c a c c c c a g g c t t t a c a c t t t a t g c t t c c g g c t c g t a t c a g t g a t a c c g c c g g c g t a a t c c g t g g g g t c c g a a a t g t g a a a t a c g a a g g c c g a g c a t a

4260 -10 1 M lac UV5 promoter CmR M a a t g t g t g g a t t t t g a g t t a g g a t c c g t c g a g a t t t t c a g g a g c t a a g g a a g c t a a a a t g t t a c a c a c c t a a a a c t c a a t c c t a g g c a g c t c t a a a a g t c c t c g a t t c c t t c g a t t t t a c

4320 5 10 15 20 EKKITGYTTVDISQWHRKEH CmR EKKITGYTTVDISQWHRKEH g a g a a a a a a a t c a c t g g a t a t a c c a c c g t t g a t a t a t c c c a a t g g c a t c g t a a a g a a c a t c t c t t t t t t t a g t g a c c t a t a t g g t g g c a a c t a t a t a g g g t t a c c g t a g c a t t t c t t g t a

4380 25 30 35 40 FEAFQSVAQCTYNQTVQLDI CmR C T C C G T A A A G T C A G T C A A C G CAT-R FEAFQSVAQCTYNQTVQLDI t t t g a g g c a t t t c a g t c a g t t g c t c a a t g t a c c t a t a a c c a g a c c g t t c a g c t g g a t a t t a a a c t c c g t a a a g t c a g t c a a c g a g t t a c a t g g a t a t t g g t c t g g c a a g t c g a c c t a t a a

4440 45 50 55 60 TAFLKTVKKNKHKFYPAFIH CmR TAFLKTVKKNKHKFYPAFIH a c g g c c t t t t t a a a g a c c g t a a a g a a a a a t a a g c a c a a g t t t t a t c c g g c c t t t a t t c a c t g c c g g a a a a a t t t c t g g c a t t t c t t t t t a t t c g t g t t c a a a a t a g g c c g g a a a t a a g t g

EcoRI 4500 65 70 75 80 ILARLMNAHPEFRMAMKDGE CmR ILARLMNAHPEFRMAMKDGE a t t c t t g c c c g c c t g a t g a a t g c t c a t c c g g a a t t c c g t a t g g c a a t g a a a g a c g g t g a g t a a g a a c g g g c g g a c t a c t t a c g a g t a g g c c t t a a g g c a t a c c g t t a c t t t c t g c c a c t c

4560 85 90 95 100 LVIWDSVHPCYTVFHEQTET CmR LVIWDSVHPCYTVFHEQTET c t g g t g a t a t g g g a t a g t g t t c a c c c t t g t t a c a c c g t t t t c c a t g a g c a a a c t g a a a c g g a c c a c t a t a c c c t a t c a c a a g t g g g a a c a a t g t g g c a a a a g g t a c t c g t t t g a c t t t g c

4620 105 110 115 120 FSSLWSEYHDDFRQFLHIYS CmR FSSLWSEYHDDFRQFLHIYS t t t t c a t c g c t c t g g a g t g a a t a c c a c g a c g a t t t c c g g c a g t t t c t a c a c a t a t a t t c g a a a a g t a g c g a g a c c t c a c t t a t g g t g c t g c t a a a g g c c g t c a a a g a t g t g t a t a t a a g c

4680 125 130 135 140 QDVACYGENLAYFPKGFIEN CmR QDVACYGENLAYFPKGFIEN c a a g a t g t g g c g t g t t a c g g t g a a a a c c t g g c c t a t t t c c c t a a a g g g t t t a t t g a g a a t g t t c t a c a c c g c a c a a t g c c a c t t t t g g a c c g g a t a a a g g g a t t t c c c a a a t a a c t c t t a

PasI 4740 145 150 155 160 MFFVSANPWVSFTSFDLNVA CmR MFFVSANPWVSFTSFDLNVA a t g t t t t t c g t c t c a g c c a a t c c c t g g g t g a g t t t c a c c a g t t t t g a t t t a a a c g t g g c c t a c a a a a a g c a g a g t c g g t t a g g g a c c c a c t c a a a g t g g t c a a a a c t a a a t t t g c a c c g g

NcoIStyIBtgI 4800 165 170 175 180 NMDNFFAPVFTMGKYYTQGD CmR NMDNFFAPVFTMGKYYTQGD a a t a t g g a c a a c t t c t t c g c c c c c g t t t t c a c c a t g g g c a a a t a t t a t a c g c a a g g c g a c t t a t a c c t g t t g a a g a a g c g g g g g c a a a a g t g g t a c c c g t t t a t a a t a t g c g t t c c g c t g

4860 185 190 195 200 KVLMPLAIQVHHAVCDGFHV CmR KVLMPLAIQVHHAVCDGFHV a a g g t g c t g a t g c c g c t g g c g a t t c a g g t t c a t c a t g c c g t c t g t g a t g g c t t c c a t g t c t t c c a c g a c t a c g g c g a c c g c t a a g t c c a a g t a g t a c g g c a g a c a c t a c c g a a g g t a c a g

4920 205 210 215 220 GRMLNELQQYCDEWQGGA* CmR GRMLNELQQYCDEWQGGA* g g c a g a a t g c t t a a t g a a t t a c a a c a g t a c t g c g a t g a g t g g c a g g g c g g g g c g t a a a c g c c g t c t t a c g a a t t a c t t a a t g t t g t c a t g a c g c t a c t c a c c g t c c c g c c c c g c a t t t g c

4980 c c g c g t g g a t c c g g c t t a c t a a a a g c c a g a t a a c a g t a t g c g t a t t t g c g c g c t g a t t t t g g c g c a c c t a g g c c g a a t g a t t t t c g g t c t a t t g t c a t a c g c a t a a a c g c g c g a c t a a a a

5040 t g c g g t a t a a g a a t a t a t a c t g a t a t g t a t a c c c g a a g t a t g t c a a a a a g a g g t a t g c t a a c g c c a t a t t c t t a t a t a t g a c t a t a c a t a t g g g c t t c a t a c a g t t t t t c t c c a t a c g a t

5100 t g a a g c a g c g t a t t a c a g t g a c a g t t g a c a g c g a c a g c t a t c a g t t g c t c a a g g c a t a t a a c t t c g t c g c a t a a t g t c a c t g t c a a c t g t c g c t g t c g a t a g t c a a c g a g t t c c g t a t a t

5160 t g a t g t c a a t a t c t c c g g t c t g g t a a g c a c a a c c a t g c a g a a t g a a g c c c g t c g t c t g c g a c t a c a g t t a t a g a g g c c a g a c c a t t c g t g t t g g t a c g t c t t a c t t c g g g c a g c a g a c g c

BbvCI 5220 t g c c g a a c g c t g g a a a g c g g a a a a t c a g g a a g g g a t g g c t g a g g t c g c c c g g t t t a t t g a a c g g c t t g c g a c c t t t c g c c t t t t a g t c c t t c c c t a c c g a c t c c a g c g g g c c a a a t a a c t

5280 1 5 MQFKVY ccdB a a t g a a c g g c t c t t t t g c t g a c g a g a a c a g g g g c t g g t g a a a t g c a g t t t a a g g t t t a c a t t a c t t g c c g a g a a a a c g a c t g c t c t t g t c c c c g a c c a c t t t a c g t c a a a t t c c a a a t g t

5340 10 15 20 25 TYKRESRYRLFVDVQSDIID ccdB c c t a t a a a a g a g a g a g c c g t t a t c g t c t g t t t g t g g a t g t a c a g a g t g a t a t t a t t g a c a g g a t a t t t t c t c t c t c g g c a a t a g c a g a c a a a c a c c t a c a t g t c t c a c t a t a a t a a c t g t

BmgBIBsaBI*SrfI 5400 30 35 40 45 TPGRRMVIPLASARLLSDKV ccdB c g c c c g g g c g a c g g a t g g t g a t c c c c c t g g c c a g t g c a c g t c t g c t g t c a g a t a a a g t c t g c g g g c c c g c t g c c t a c c a c t a g g g g g a c c g g t c a c g t g c a g a c g a c a g t c t a t t t c a g a

5460 50 55 60 65 SRELYPVVHIGDESWRMMTT ccdB G C A T G A T G A C C A C C G ccdB-fwd c c c g t g a a c t t t a c c c g g t g g t g c a t a t c g g g g a t g a a a g c t g g c g c a t g a t g a c c a c c g g g g c a c t t g a a a t g g g c c a c c a c g t a t a g c c c c t a c t t t c g a c c g c g t a c t a c t g g t g g c

5520 70 75 80 85 DMASVPVSVIGEEVADLSHR ccdB A T A T G ccdB-fwd a t a t g g c c a g t g t g c c g g t c t c c g t t a t c g g g g a a g a a g t g g c t g a t c t c a g c c a c c g c g t a t a c c g g t c a c a c g g c c a g a g g c a a t a g c c c c t t c t t c a c c g a c t a g a g t c g g t g g c g c

5580 90 95 100 ENDIKNAINLMFWGI* ccdB a a a a t g a c a t c a a a a a c g c c a t t a a c c t g a t g t t c t g g g g a a t a t a a a t g t c a g g c t c c c t t t t a c t g t a g t t t t t g c g g t a a t t g g a c t a c a a g a c c c c t t a t a t t t a c a g t c c g a g g g

PstISalI 5640 attR2 t t a t a c a c a g c c a g t c t g c a g g t c g a c c a t a g t g a c t g g a t a t g t t g t g t t t t a c a g t a t a a t a t g t g t c g g t c a g a c g t c c a g c t g g t a t c a c t g a c c t a t a c a a c a c a a a a t g t c a t a

5700 attR2 t a t g t a g t c t g t t t t t t a t g c a a a a t c t a a t t t a a t a t a t t g a t a t t t a t a t c a t t t t a c a t a c a t c a g a c a a a a a a t a c g t t t t a g a t t a a a t t a t a t a a c t a t a a a t a t a g t a a a a t g

5760 attR2 CYC1 terminator C A G T G C CYC1 g t t t c t c g t t c a g c t t t c t t g t a c a a a g t g g t t c g a t g t c a t g t a a t t a g t t a t g t c a c g c a a a g a g c a a g t c g a a a g a a c a t g t t t c a c c a a g c t a c a g t a c a t t a a t c a a t a c a g t g c

5820 CYC1 terminator G A A T G T A A G T G C G CYC1 c t t a c a t t c a c g c c c t c c c c c c a c a t c c g c t c t a a c c g a a a a g g a a g g a g t t a g a c a a c c g a a t g t a a g t g c g g g a g g g g g g t g t a g g c g a g a t t g g c t t t t c c t t c c t c a a t c t g t t g g

5880 CYC1 terminator t g a a g t c t a g g t c c c t a t t t a t t t t t t t a t a g t t a t g t t a g t a t t a a g a a c g t t a t t t a t a c t t c a g a t c c a g g g a t a a a t a a a a a a a t a t c a a t a c a a t c a t a a t t c t t g c a a t a a a t a

MluI 5940 CYC1 terminator a t t t c a a a t t t t t c t t t t t t t t c t g t a c a g a c g c g t g t a c g c a t g t a a c a t t a t a c t g a a t a a a g t t t a a a a a g a a a a a a a a g a c a t g t c t g c g c a c a t g c g t a c a t t g t a a t a t g a c t t

Acc65IKpnI 6000 CYC1 terminator a a c c t t g c t t g a g a a g g t t t t g g g a c g c t c g a a g g c t t t a a t t t g c g g c c g g t a c c c a a t t t g g a a c g a a c t c t t c c a a a a c c c t g c g a g c t t c c g a a a t t a a a c g c c g g c c a t g g g t t a

6060 T7 promoter M13 fwd G G G A T A T C A C T C A G C A T A A T T7 T G A C C G G C A G C A A A A T G T M13 Forward G C A A A A T G T T G C A G C A C T G M13/pUC Forward t c g c c c t a t a g t g a g t c g t a t t a c g c g c g c t c a c t g g c c g t c g t t t t a c a a c g t c g t g a c a g c g g g a t a t c a c t c a g c a t a a t g c g c g c g a g t g a c c g g c a g c a a a a t g t t g c a g c a c t g

6120 A C C C M13/pUC Forward t g g g a a a a c c c t g g c g t t a c c c a a c t t a a t c g c c t t g c a g c a c a t c c c c c t t t c g c c a g c a c c c t t t t g g g a c c g c a a t g g g t t g a a t t a g c g g a a c g t c g t g t a g g g g g a a a g c g g t c g

6180 t g g c g t a a t a g c g a a g a g g c c c g c a c c g a t c g c c c t t c c c a a c a g t t g c g c a g c c t g a a t a c c g c a t t a t c g c t t c t c c g g g c g t g g c t a g c g g g a a g g g t t g t c a a c g c g t c g g a c t t a

6240 f1 ori g g c g a a t g g c g c g a c g c g c c c t g t a g c g g c g c a t t a a g c g c g g c g g g t g t g g t g g t t a c g c c g c t t a c c g c g c t g c g c g g g a c a t c g c c g c g t a a t t c g c g c c g c c c a c a c c a c c a a t g c

6300 f1 ori G A G G A A A G C G A A A G A A G G G A F1ori-R c g c a g c g t g a c c g c t a c a c t t g c c a g c g c c c t a g c g c c c g c t c c t t t c g c t t t c t t c c c t g c g t c g c a c t g g c g a t g t g a a c g g t c g c g g g a t c g c g g g c g a g g a a a g c g a a a g a a g g g a

NgoMIVNaeI 6360 f1 ori t c c t t t c t c g c c a c g t t c g c c g g c t t t c c c c g t c a a g c t c t a a a t c g g g g g c t c c c t t t a a g g a a a g a g c g g t g c a a g c g g c c g a a a g g g g c a g t t c g a g a t t t a g c c c c c g a g g g a a a t

6420 f1 ori g g g t t c c g a t t t a g t g c t t t a c g g c a c c t c g a c c c c a a a a a a c t t g a t t a g g g t g a t g g t c c c a a g g c t a a a t c a c g a a a t g c c g t g g a g c t g g g g t t t t t t g a a c t a a t c c c a c t a c c a

DraIII 6480 f1 ori t c a c g t a g t g g g c c a t c g c c c t g a t a g a c g g t t t t t c g c c c t t t g a c g t t g g a g t c c a c g a g t g c a t c a c c c g g t a g c g g g a c t a t c t g c c a a a a a g c g g g a a a c t g c a a c c t c a g g t g c

6540 f1 ori G T G G A C T C T T G T T C C A A A C T G G F1ori-F t t c t t t a a t a g t g g a c t c t t g t t c c a a a c t g g a a c a a c a c t c a a c c c t a t c t c g g t c t a t a a g a a a t t a t c a c c t g a g a a c a a g g t t t g a c c t t g t t g t g a g t t g g g a t a g a g c c a g a t a

6600 f1 ori t c t t t t g a t t t a t a a g g g a t t t t g c c g a t t t c g g c c t a t t g g t t a a a a a a t g a g c t g a t t a g a a a a c t a a a t a t t c c c t a a a a c g g c t a a a g c c g g a t a a c c a a t t t t t t a c t c g a c t a a

6660 f1 ori t a a c a a a a a t t t a a c g c g a a t t t t a a c a a a a t a t t a a c g t t t a c a a t t t c c t g a t g c g g t a t t g t t t t t a a a t t g c g c t t a a a a t t g t t t t a t a a t t g c a a a t g t t a a a g g a c t a c g c c a

6720 a t t t t c t c c t t a c g c a t c t g t g c g g t a t t t c a c a c c g c a t a g g c a a g t g c a c a a a c a a t a t a a a a g a g g a a t g c g t a g a c a c g c c a t a a a g t g t g g c g t a t c c g t t c a c g t g t t t g t t a t

6780 225 220 215 *KKANKVFNAI TRP1 *KKANKVFNAI c t t a a a t a a a t a c t a c t c a g t a a t a a c c t a t t t c t t a g c a t t t t t g a c g a a a t t t g c t a t g a a t t t a t t t a t g a t g a g t c a t t a t t g g a t a a a g a a t c g t a a a a a c t g c t t t a a a c g a t a

6840 210 205 200 195 KNSDKVGNTEVGGSVDVGIV TRP1 KNSDKVGNTEVGGSVDVGIV t t t g t t a g a g t c t t t t a c a c c a t t t g t c t c c a c a c c t c c g c t t a c a t c a a c a c c a a t a a c a a a c a a t c t c a g a a a a t g t g g t a a a c a g a g g t g t g g a g g c g a a t g t a g t t g t g g t t a t t g

6900 190 185 180 175 GNLRLADGVNEPTLGGALMF TRP1 GNLRLADGVNEPTLGGALMF g c c a t t t a a t c t a a g c g c a t c a c c a a c a t t t t c t g g c g t c a g t c c a c c a g c t a a c a t a a a c g g t a a a t t a g a t t c g c g t a g t g g t t g t a a a a g a c c g c a g t c a g g t g g t c g a t t g t a t t t

HindIII 6960 170 165 160 155 HLSEPSEQRGVWDSISNWDL TRP1 HLSEPSEQRGVWDSISNWDL a t g t a a g c t t t c g g g g c t c t c t t g c c t t c c a a c c c a g t c a g a a a t c g a g t t c c a a t c c a a t a c a t t c g a a a g c c c c g a g a g a a c g g a a g g t t g g g t c a g t c t t t a g c t c a a g g t t a g g t t

PaqCI 7020 150 145 140 135 LEGTGGAESDFLPIFSHPKQ TRP1 LEGTGGAESDFLPIFSHPKQ a a g t t c a c c t g t c c c a c c t g c t t c t g a a t c a a a c a a g g g a a t a a a c g a a t g a g g t t t c t g t t c a a g t g g a c a g g g t g g a c g a a g a c t t a g t t t g t t c c c t t a t t t g c t t a c t c c a a a g a c

7080 130 125 120 115 SAASLLINCDKPFVLRKIVP TRP1 SAASLLINCDKPFVLRKIVP t g a a g c t g c a c t g a g t a g t a t g t t g c a g t c t t t t g g a a a t a c g a g t c t t t t a a t a a c t g g a c t t c g a c g t g a c t c a t c a t a c a a c g t c a g a a a a c c t t t a t g c t c a g a a a a t t a t t g a c c

EcoRV 7140 110 105 100 95 LGLFEQYEQWSEDGHLQVID TRP1 LGLFEQYEQWSEDGHLQVID c a a a c c g a g g a a c t c t t g g t a t t c t t g c c a c g a c t c a t c t c c a t g c a g t t g g a c g a t a t c g t t t g g c t c c t t g a g a a c c a t a a g a a c g g t g c t g a g t a g a g g t a c g t c a a c c t g c t a t a g

Bsu36I 7200 90 85 80 75 IGYDNVLALVDEKPQNRFVG TRP1 IGYDNVLALVDEKPQNRFVG a a t g c c g t a a t c a t t g a c c a g a g c c a a a a c a t c c t c c t t a g g t t g a t t a c g a a a c a c g c c t t a c g g c a t t a g t a a c t g g t c t c g g t t t t g t a g g a g g a a t c c a a c t a a t g c t t t g t g c g g

7260 70 65 60 55 VLYKPTGSSNKYAKVLSSIK TRP1 VLYKPTGSSNKYAKVLSSIK a a c c a a g t a t t t c g g a g t g c c t g a a c t a t t t t t a t a t g c t t t t a c a a g a c t t g a a a t t t t t t g g t t c a t a a a g c c t c a c g g a c t t g a t a a a a a t a t a c g a a a a t g t t c t g a a c t t t a a a a

MfeI 7320 50 45 40 35 RAIVPDITRKRNPVCIIGLL TRP1 RAIVPDITRKRNPVCIIGLL c c t t g c a a t a a c c g g g t c a a t t g t t c t c t t t c t a t t g g g c a c a c a t a t a a t a c c c a g c a a g g a a c g t t a t t g g c c c a g t t a a c a a g a g a a a g a t a a c c c g t g t g t a t a t t a t g g g t c g t t

BstAPI 7380 30 25 20 15 DADSDLACEAAETSQLGCVK TRP1 DADSDLACEAAETSQLGCVK g t c a g c a t c g g a a t c t a g a g c a c a t t c t g c g g c c t c t g t g c t c t g c a a g c c g c a a a c t t t c a g t c g t a g c c t t a g a t c t c g t g t a a g a c g c c g g a g a c a c g a g a c g t t c g g c g t t t g a a a

7440 10 5 1 VLPGSSGTFNIVSM TRP1 TRP1 promoter VLPGSSGTFNIVSM c a c c a a t g g a c c a g a a c t a c c t g t g a a a t t a a t a a c a g a c a t a c t c c a a g c t g c c t t t g t g t g g t t a c c t g g t c t t g a t g g a c a c t t t a a t t a t t g t c t g t a t g a g g t t c g a c g g a a a c a

7500 TRP1 promoter g t g c t t a a t c a c g t a t a c t c a c g t g c t c a a t a g t c a c c a a t g c c c t c c c t c t t g g c c c t c c a c g a a t t a g t g c a t a t g a g t g c a c g a g t t a t c a g t g g t t a c g g g a g g g a g a a c c g g g a g

7560 TRP1 promoter t c c t t t t c t t t t t t c g a c c g a a t t a a t t c t t a a t c g g c a a a a a a a g a a a a g c t c c g g a t c a g g a a a a g a a a a a a g c t g g c t t a a t t a a g a a t t a g c c g t t t t t t t c t t t t c g a g g c c t a g

SnaBI 7620 TRP1 promoter a a g a t t g t a c g t a a g g t g a c a a g c t a t t t t t c a a t a a a g a a t a t c t t c c a c t a c t g c c a t t t c t a a c a t g c a t t c c a c t g t t c g a t a a a a a g t t a t t t c t t a t a g a a g g t g a t g a c g g t a

7680 TRP1 promoter c t g g c g t c a t a a c t g c a a a g t a c a c a t a t a t t a c g a t g c t g t c t a t t a a a t g c t t c c t a t g a c c g c a g t a t t g a c g t t t c a t g t g t a t a t a a t g c t a c g a c a g a t a a t t t a c g a a g g a t a

7740 TRP1 promoter A T G T T A G A C G A G A C T A C G G C pRS-marker a t t a t a t a t a t a g t a a t g t c g t t t a t g g t g c a c t c t c a g t a c a a t c t g c t c t g a t g c c g c t a a t a t a t a t a t c a t t a c a g c a a a t a c c a c g t g a g a g t c a t g t t a g a c g a g a c t a c g g c g

7800 a t a g t t a a g c c a g c c c c g a c a c c c g c c a a c a c c c g c t g a c g c g c c c t g a c g g g c t t g t c t t a t c a a t t c g g t c g g g g c t g t g g g c g g t t g t g g g c g a c t g c g c g g g a c t g c c c g a a c a g a

7860 C C G G G A G C T G C A T G T G T C A G A G pGEX 3' g c t c c c g g c a t c c g c t t a c a g a c a a g c t g t g a c c g t c t c c g g g a g c t g c a t g t g t c a g a g c g a g g g c c g t a g g c g a a t g t c t g t t c g a c a c t g g c a g a g g c c c t c g a c g t a c a c a g t c t c

3ʹ 5ʹ 7889 G pGEX 3' g t t t t c a c c g t c a t c a c c g a a a c g c g c g a c a a a a g t g g c a g t a g t g g c t t t g c g c g c t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.