Addgene pAG425GPD-EYFP-ccdB - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pAG425GPD-EYFP-ccdB


Map View

pGEX 3' (10,099 .. 10,121) pRS-marker (9980 .. 9999) PflFI - Tth111I (9956) EcoRV (9142) AflII (8707) AgeI (8642) BspDI - ClaI (8544) BstEII (8426) HpaI (7975) PluTI (7946) SfoI (7944) NarI (7943) KasI (7942) F1ori-F (7518 .. 7539) DraIII (7454) NaeI (7348) NgoMIV (7346) F1ori-R (7308 .. 7327) M13/pUC Forward (7069 .. 7091) M13 Forward (7060 .. 7077) M13 fwd T7 (7031 .. 7050) T7 promoter MluI (6938) CYC1 (6782 .. 6800) SalI (6629) ccdB-fwd (6473 .. 6492) BsaBI * (6385) SrfI (6372) BbvCI (6226) BstZ17I (6036) NcoI (5799) PasI (5730) BspEI (5494) CAT-R (5351 .. 5370) lac UV5 promoter NotI (5178) pBRforEco (10 .. 28) NsiI (1125) Amp-R (1761 .. 1780) AhdI (2330) pBR322ori-F (3063 .. 3082) L4440 (3316 .. 3333) M13/pUC Reverse (3529 .. 3551) lac operator M13 Reverse (3548 .. 3564) M13 rev T3 (3583 .. 3603) Eco53kI (3618) SacI (3620) GPDpro-F (4170 .. 4193) pBluescriptSK (4282 .. 4298) SpeI (4288) SK primer EGFP-N (4356 .. 4377) EXFP-R (4617 .. 4636) EGFP-C (4964 .. 4985) CsiI - SexAI * (5033) pAG425GPD-EYFP-ccdB 10,149 bp

Sequence View

5ʹ 3ʹ 60 C G G A G C A C T A T G C G G A T A A pBRforEco g a c g a a a g g g c c t c g t g a t a c g c c t a t t t t t a t a g g t t a a t g t c a t g a t a a t a a t g g t t t c t g c t t t c c c g g a g c a c t a t g c g g a t a a a a a t a t c c a a t t a c a g t a c t a t t a t t a c c a a a

120 2μ ori c t t a g t a t g a t c c a a t a t c a a a g g a a a t g a t a g c a t t g a a g g a t g a g a c t a a t c c a a t t g g a a t c a t a c t a g g t t a t a g t t t c c t t t a c t a t c g t a a c t t c c t a c t c t g a t t a g g t t a a c

180 2μ ori a g g a g t g g c a g c a t a t a g a a c a g c t a a a g g g t a g t g c t g a a g g a a g c a t a c g a t a c c c c g t c c t c a c c g t c g t a t a t c t t g t c g a t t t c c c a t c a c g a c t t c c t t c g t a t g c t a t g g g g c

240 2μ ori c a t g g a a t g g g a t a a t a t c a c a g g a g g t a c t a g a c t a c c t t t c a t c c t a c a t a a a t a g a c g t a c c t t a c c c t a t t a t a g t g t c c t c c a t g a t c t g a t g g a a a g t a g g a t g t a t t t a t c t g

300 2μ ori g c a t a t a a g t a c g c a t t t a a g c a t a a a c a c g c a c t a t g c c g t t c t t c t c a t g t a t a t a t a c g t a t a t t c a t g c g t a a a t t c g t a t t t g t g c g t g a t a c g g c a a g a a g a g t a c a t a t a t a t

360 2μ ori t a t a c a g g c a a c a c g c a g a t a t a g g t g c g a c g t g a a c a g t g a g c t g t a t g t g c g c a g c t c a t a t g t c c g t t g t g c g t c t a t a t c c a c g c t g c a c t t g t c a c t c g a c a t a c a c g c g t c g a g

420 2μ ori FRT g c g t t g c a t t t t c g g a a g c g c t c g t t t t c g g a a a c g c t t t g a a g t t c c t a t t c c g a a g t t c g c a a c g t a a a a g c c t t c g c g a g c a a a a g c c t t t g c g a a a c t t c a a g g a t a a g g c t t c a a

480 2μ ori FRT c c t a t t c t c t a g a a a g t a t a g g a a c t t c a g a g c g c t t t t g a a a a c c a a a a g c g c t c t g a a g g a t a a g a g a t c t t t c a t a t c c t t g a a g t c t c g c g a a a a c t t t t g g t t t t c g c g a g a c t t

540 2μ ori g a c g c a c t t t c a a a a a a c c a a a a a c g c a c c g g a c t g t a a c g a g c t a c t a a a a t a t t g c g a c t g c g t g a a a g t t t t t t g g t t t t t g c g t g g c c t g a c a t t g c t c g a t g a t t t t a t a a c g c t

600 2μ ori a t a c c g c t t c c a c a a a c a t t g c t c a a a a g t a t c t c t t t g c t a t a t a t c t c t g t g c t a t a t t a t g g c g a a g g t g t t t g t a a c g a g t t t t c a t a g a g a a a c g a t a t a t a g a g a c a c g a t a t a

660 2μ ori c c c t a t a t a a c c t a c c c a t c c a c c t t t c g c t c c t t g a a c t t g c a t c t a a a c t c g a c c t c t g g g a t a t a t t g g a t g g g t a g g t g g a a a g c g a g g a a c t t g a a c g t a g a t t t g a g c t g g a g a

720 2μ ori a c a t t t t t t a t g t t t a t c t c t a g t a t t a c t c t t t a g a c a a a a a a a t t g t a g t a a g a a c t a t g t a a a a a a t a c a a a t a g a g a t c a t a a t g a g a a a t c t g t t t t t t t a a c a t c a t t c t t g a t

780 2μ ori t t c a t a g a g t g a a t c g a a a a c a a t a c g a a a a t g t a a a c a t t t c c t a t a c g t a g t a t a t a g a a g t a t c t c a c t t a g c t t t t g t t a t g c t t t t a c a t t t g t a a a g g a t a t g c a t c a t a t a t c

840 2μ ori a g a c a a a a t a g a a g a a a c c g t t c a t a a t t t t c t g a c c a a t g a a g a a t c a t c a a c g c t a t c t c t g t t t t a t c t t c t t t g g c a a g t a t t a a a a g a c t g g t t a c t t c t t a g t a g t t g c g a t a g

900 2μ ori a c t t t c t g t t c a c a a a g t a t g c g c a a t c c a c a t c g g t a t a g a a t a t a a t c g g g g a t g c c t t g a a a g a c a a g t g t t t c a t a c g c g t t a g g t g t a g c c a t a t c t t a t a t t a g c c c c t a c g g a

960 2μ ori t t a t c t t g a a a a a a t g c a c c c g c a g c t t c g c t a g t a a t c a g t a a a c g c g g g a a g t g g a g t a a t a g a a c t t t t t t a c g t g g g c g t c g a a g c g a t c a t t a g t c a t t t g c g c c c t t c a c c t c a

1020 2μ ori c a g g c t t t t t t t a t g g a a g a g a a a a t a g a c a c c a a a g t a g c c t t c t t c t a a c c t t a a c g g g t c c g a a a a a a a t a c c t t c t c t t t t a t c t g t g g t t t c a t c g g a a g a a g a t t g g a a t t g c c

1080 2μ ori a c c t a c a g t g c a a a a a g t t a t c a a g a g a c t g c a t t a t a g a g c g c a c a a a g g a g a a a a a a a t g g a t g t c a c g t t t t t c a a t a g t t c t c t g a c g t a a t a t c t c g c g t g t t t c c t c t t t t t t t

NsiI 1140 2μ ori g t a a t c t a a g a t g c t t t g t t a g a a a a a t a g c g c t c t c g g g a t g c a t t t t t g t a g a a c a a a c a t t a g a t t c t a c g a a a c a a t c t t t t t a t c g c g a g a g c c c t a c g t a a a a a c a t c t t g t t t

1200 2μ ori a a a g a a g t a t a g a t t c t t t g t t g g t a a a a t a g c g c t c t c g c g t t g c a t t t c t g t t c t g t a t t t c t t c a t a t c t a a g a a a c a a c c a t t t t a t c g c g a g a g c g c a a c g t a a a g a c a a g a c a t

1260 2μ ori a a a a t g c a g c t c a g a t t c t t t g t t t g a a a a a t t a g c g c t c t c g c g t t g c a t t t t t g t t t t t t t t a c g t c g a g t c t a a g a a a c a a a c t t t t t a a t c g c g a g a g c g c a a c g t a a a a a c a a a a

1320 2μ ori a c a a a a a t g a a g c a c a g a t t c t t c g t t g g t a a a a t a g c g c t t t c g c g t t g c a t t t c t g t t t g t t t t t a c t t c g t g t c t a a g a a g c a a c c a t t t t a t c g c g a a a g c g c a a c g t a a a g a c a a

1380 2μ ori c t g t a a a a a t g c a g c t c a g a t t c t t t g t t t g a a a a a t t a g c g c t c t c g c g t t g c a t t t t t g a c a t t t t t a c g t c g a g t c t a a g a a a c a a a c t t t t t a a t c g c g a g a g c g c a a c g t a a a a a

1440 2μ ori AmpR promoter g t t c t a c a a a a t g a a g c a c a g a t g c t t c g t t c a g g t g g c a c t t t t c g g g g a a a t g t g c g c c a a g a t g t t t t a c t t c g t g t c t a c g a a g c a a g t c c a c c g t g a a a a g c c c c t t t a c a c g c g

1500 AmpR promoter g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t

1560 1 5 signal sequence MSIQHF AmpR promoter AmpR MSIQHF t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t a t g a g t a t t c a a c a t t t c a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a t a c t c a t a a g t t g t a a a g

1620 10 15 20 signal sequence RVALIPFFAAFCLPVFA 25 HPE AmpR RVALIPFFAAFCLPVFAHPE c g t g t c g c c c t t a t t c c c t t t t t t g c g g c a t t t t g c c t t c c t g t t t t t g c t c a c c c a g a a g c a c a g c g g g a a t a a g g g a a a a a a c g c c g t a a a a c g g a a g g a c a a a a a c g a g t g g g t c t t

1680 30 35 40 45 TLVKVKDAEDQLGARVGYIE AmpR TLVKVKDAEDQLGARVGYIE a c g c t g g t g a a a g t a a a a g a t g c t g a a g a t c a g t t g g g t g c a c g a g t g g g t t a c a t c g a a t g c g a c c a c t t t c a t t t t c t a c g a c t t c t a g t c a a c c c a c g t g c t c a c c c a a t g t a g c t t

1740 50 55 60 65 LDLNSGKILESFRPEERFPM AmpR LDLNSGKILESFRPEERFPM c t g g a t c t c a a c a g c g g t a a g a t c c t t g a g a g t t t t c g c c c c g a a g a a c g t t t t c c a a t g g a c c t a g a g t t g t c g c c a t t c t a g g a a c t c t c a a a a g c g g g g c t t c t t g c a a a a g g t t a c

1800 70 75 80 85 MSTFKVLLCGAVLSRIDAGQ AmpR C G A T A C A C C G C G C C A T A A T A Amp-R MSTFKVLLCGAVLSRIDAGQ a t g a g c a c t t t t a a a g t t c t g c t a t g t g g c g c g g t a t t a t c c c g t a t t g a c g c c g g g c a a t a c t c g t g a a a a t t t c a a g a c g a t a c a c c g c g c c a t a a t a g g g c a t a a c t g c g g c c c g t t

1860 90 95 100 105 EQLGRRIHYSQNDLVEYSPV AmpR EQLGRRIHYSQNDLVEYSPV g a g c a a c t c g g t c g c c g c a t a c a c t a t t c t c a g a a t g a c t t g g t t g a g t a c t c a c c a g t c c t c g t t g a g c c a g c g g c g t a t g t g a t a a g a g t c t t a c t g a a c c a a c t c a t g a g t g g t c a g

1920 110 115 120 125 TEKHLTDGMTVRELCSAAIT AmpR TEKHLTDGMTVRELCSAAIT a c a g a a a a g c a t c t t a c g g a t g g c a t g a c a g t a a g a g a a t t a t g c a g t g c t g c c a t a a c c t g t c t t t t c g t a g a a t g c c t a c c g t a c t g t c a t t c t c t t a a t a c g t c a c g a c g g t a t t g g

1980 130 135 140 145 MSDNTAANLLLTTIGGPKEL AmpR MSDNTAANLLLTTIGGPKEL a t g a g t g a t a a c a c t g c g g c c a a c t t a c t t c t g a c a a c g a t c g g a g g a c c g a a g g a g c t a t a c t c a c t a t t g t g a c g c c g g t t g a a t g a a g a c t g t t g c t a g c c t c c t g g c t t c c t c g a t

2040 150 155 160 165 TAFLHNMGDHVTRLDRWEPE AmpR TAFLHNMGDHVTRLDRWEPE a c c g c t t t t t t g c a c a a c a t g g g g g a t c a t g t a a c t c g c c t t g a t c g t t g g g a a c c g g a g t g g c g a a a a a a c g t g t t g t a c c c c c t a g t a c a t t g a g c g g a a c t a g c a a c c c t t g g c c t c

2100 170 175 180 185 LNEAIPNDERDTTMPVAMAT AmpR LNEAIPNDERDTTMPVAMAT c t g a a t g a a g c c a t a c c a a a c g a c g a g c g t g a c a c c a c g a t g c c t g t a g c a a t g g c a a c a g a c t t a c t t c g g t a t g g t t t g c t g c t c g c a c t g t g g t g c t a c g g a c a t c g t t a c c g t t g t

2160 190 195 200 205 TLRKLLTGELLTLASRQQLI AmpR TLRKLLTGELLTLASRQQLI a c g t t g c g c a a a c t a t t a a c t g g c g a a c t a c t t a c t c t a g c t t c c c g g c a a c a a t t a a t a t g c a a c g c g t t t g a t a a t t g a c c g c t t g a t g a a t g a g a t c g a a g g g c c g t t g t t a a t t a t

2220 210 215 220 225 DWMEADKVAGPLLRSALPAG AmpR DWMEADKVAGPLLRSALPAG g a c t g g a t g g a g g c g g a t a a a g t t g c a g g a c c a c t t c t g c g c t c g g c c c t t c c g g c t g g c c t g a c c t a c c t c c g c c t a t t t c a a c g t c c t g g t g a a g a c g c g a g c c g g g a a g g c c g a c c g

2280 230 235 240 245 WFIADKSGAGERGSRGIIAA AmpR WFIADKSGAGERGSRGIIAA t g g t t t a t t g c t g a t a a a t c t g g a g c c g g t g a g c g t g g g t c t c g c g g t a t c a t t g c a g c a a c c a a a t a a c g a c t a t t t a g a c c t c g g c c a c t c g c a c c c a g a g c g c c a t a g t a a c g t c g t

AhdI 2340 250 255 260 265 LGPDGKPSRIVVIYTTGSQA AmpR LGPDGKPSRIVVIYTTGSQA c t g g g g c c a g a t g g t a a g c c c t c c c g t a t c g t a g t t a t c t a c a c g a c g g g g a g t c a g g c a g a c c c c g g t c t a c c a t t c g g g a g g g c a t a g c a t c a a t a g a t g t g c t g c c c c t c a g t c c g t

2400 270 275 280 285 TMDERNRQIAEIGASLIKHW AmpR TMDERNRQIAEIGASLIKHW a c t a t g g a t g a a c g a a a t a g a c a g a t c g c t g a g a t a g g t g c c t c a c t g a t t a a g c a t t g g t g a t a c c t a c t t g c t t t a t c t g t c t a g c g a c t c t a t c c a c g g a g t g a c t a a t t c g t a a c c

2460 * AmpR * t a a c t g t c a g a c c a a g t t t a c t c a t a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a a t t g a c a g t c t g g t t c a a a t g a g t a t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t

2520 t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a

2580 ori g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a

2640 ori c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c

2700 ori g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t

2760 ori g c g c a g a t a c c a a a t a c t g t c c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c c g c g t c t a t g g t t t a t g a c a g g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g

2820 ori t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a

2880 ori g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c

2940 ori c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g

3000 ori g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c

3060 ori g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t

3120 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a

3180 ori c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g

3240 t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g

3300 c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c t t t g a g t g a g c t g a t a c c g c t c g c c g c a g c g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c g a c t a t g g c g a g c g g c g t c g

3360 A G C G A G T C A G T G A G C G A G L4440 c g a a c g a c c g a g c g c a g c g a g t c a g t g a g c g a g g a a g c g g a a g a g c g c c c a a t a c g c a a a g c t t g c t g g c t c g c g t c g c t c a g t c a c t c g c t c c t t c g c c t t c t c g c g g g t t a t g c g t t t

3420 c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g g c a c g a c a g g t t t c c c g a c g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c c g t g c t g t c c a a a g g g c t g

3480 t g g a a a g c g g g c a g t g a g c g c a a c g c a a t t a a t g t g a g t t a c c t c a c t c a t t a g g c a c c c a c c t t t c g c c c g t c a c t c g c g t t g c g t t a a t t a c a c t c a a t g g a g t g a g t a a t c c g t g g g

3540 -35 -10 lac promoter lac operator A G C G G A T A A C A A M13/pUC Reverse c a g g c t t t a c a c t t t a t g c t t c c g g c t c c t a t g t t g t g t g g a a t t g t g a g c g g a t a a c a a g t c c g a a a t g t g a a a t a c g a a g g c c g a g g a t a c a a c a c a c c t t a a c a c t c g c c t a t t g t t

3600 M13 rev T3 promoter T T T C A C A C A G G M13/pUC Reverse C A G G A A A C A G C T A T G A C M13 Reverse G C A A T T A A C C C T C A C T A A T3 t t t c a c a c a g g a a a c a g c t a t g a c c a t g a t t a c g c c a a g c g c g c a a t t a a c c c t c a c t a a a a a g t g t g t c c t t t g t c g a t a c t g g t a c t a a t g c g g t t c g c g c g t t a a t t g g g a g t g a t t

Eco53kISacI 3660 T3 promoter GAP promoter A G G T3 a g g g a a c a a a a g c t g g a g c t c t t c g a g t t t a t c a t t a t c a a t a c t c g c c a t t t c a a a g a a t c c c t t g t t t t c g a c c t c g a g a a g c t c a a a t a g t a a t a g t t a t g a g c g g t a a a g t t t c t t

3720 GAP promoter t a c g t a a a t a a t t a a t a g t a g t g a t t t t c c t a a c t t t a t t t a g t c a a a a a a t t a g c c t t t a t g c a t t t a t t a a t t a t c a t c a c t a a a a g g a t t g a a a t a a a t c a g t t t t t t a a t c g g a a a

3780 GAP promoter t a a t t c t g c t g t a a c c c g t a c a t g c c c a a a a t a g g g g g c g g g t t a c a c a g a a t a t a t a a c a t t a a g a c g a c a t t g g g c a t g t a c g g g t t t t a t c c c c c g c c c a a t g t g t c t t a t a t a t t g

3840 GAP promoter a c c g a a g g t g c c t g g g t g a a c a g g t t t a t t c c t g g c a t c c a c t a a a t a t a a t g g a g c c c g t g g c t t c c a c g g a c c c a c t t g t c c a a a t a a g g a c c g t a g g t g a t t t a t a t t a c c t c g g g c

3900 GAP promoter c t t t t t a a g c t g g c a t c c a g a a a a a a a a a g a a t c c c a g c a c c a a a a t a t t g t t t t c t t c a g a a a a a t t c g a c c g t a g g t c t t t t t t t t t c t t a g g g t c g t g g t t t t a t a a c a a a a g a a g t

3960 GAP promoter c c a a c c a t c a g t t c a t a g g t c c a t t c t c t t a g c g c a a c t a c a g a g a a c a g g g g c a c a a a c g g t t g g t a g t c a a g t a t c c a g g t a a g a g a a t c g c g t t g a t g t c t c t t g t c c c c g t g t t t g

4020 GAP promoter a g g c a a a a a a c g g g c a c a a c c t c a a t g g a g t g a t g c a a c c t g c c t g g a g t a a a t g a t g a c t c c g t t t t t t g c c c g t g t t g g a g t t a c c t c a c t a c g t t g g a c g g a c c t c a t t t a c t a c t g

4080 GAP promoter a c a a g g c a a t t g a c c c a c g c a t g t a t c t a t c t c a t t t t c t t a c a c c t t c t a t t a c c t t c t t g t t c c g t t a a c t g g g t g c g t a c a t a g a t a g a g t a a a a g a a t g t g g a a g a t a a t g g a a g a

4140 GAP promoter g c t c t c t c t g a t t t g g a a a a a g c t g a a a a a a a a g g t t g a a a c c a g t t c c c t g a a a t t a t t c g a g a g a g a c t a a a c c t t t t t c g a c t t t t t t t t c c a a c t t t g g t c a a g g g a c t t t a a t a a

4200 GAP promoter C G G T A G G T A T T G A T T G T A A T T C T G GPDpro-F c c c c t a c t t g a c t a a t a a g t a t a t a a a g a c g g t a g g t a t t g a t t g t a a t t c t g t a a a t c t g g g g a t g a a c t g a t t a t t c a t a t a t t t c t g c c a t c c a t a a c t a a c a t t a a g a c a t t t a g a

4260 GAP promoter *IRSKYNTKKKTKFC a t t t c t t a a a c t t c t t a a a t t c t a c t t t t a t a g t t a g t c t t t t t t t t a g t t t t a a a a c a c t a a a g a a t t t g a a g a a t t t a a g a t g a a a a t a t c a a t c a g a a a a a a a a t c a a a a t t t t g t g

SpeI 4320 1 M 1a V S GAP promoter SK primer EYFP T C T A G A A C T A G T G G A T C pBluescriptSK MVS WFKTEVSELVLPDGPVFHHA c a g a a c t t a g t t t c g a c g g a t t c t a g a a c t a g t g g a t c c c c c g g g a c a a a a t g g t g a g c a g t c t t g a a t c a a a g c t g c c t a a g a t c t t g a t c a c c t a g g g g g c c c t g t t t t a c c a c t c g t

4380 5 10 15 20 KGEELFTGVVPILVELDGDV EYFP G A C C A G C T C G A C C T G C C G C T G C EGFP-N KGEELFTGVVPILVELDGDV LALLQEGPHHGDQDLQVAVY a g g g c g a g g a g c t g t t c a c c g g g g t g g t g c c c a t c c t g g t c g a g c t g g a c g g c g a c g t a a t c c c g c t c c t c g a c a a g t g g c c c c a c c a c g g g t a g g a c c a g c t c g a c c t g c c g c t g c a t t

4440 25 30 35 40 NGHKFSVSGEGEGDATYGKL EYFP NGHKFSVSGEGEGDATYGKL VAVLEAHGALALAIGGVALQ a c g g c c a c a a g t t c a g c g t g t c c g g c g a g g g c g a g g g c g a t g c c a c c t a c g g c a a g c t g a t g c c g g t g t t c a a g t c g c a c a g g c c g c t c c c g c t c c c g c t a c g g t g g a t g c c g t t c g a c t

4500 45 50 55 60 TLKFICTTGKLPVPWPTLVT EYFP TLKFICTTGKLPVPWPTLVT GQLEDAGGALQGHGPGGEHG c c c t g a a g t t c a t c t g c a c c a c c g g c a a g c t g c c c g t g c c c t g g c c c a c c c t c g t g a c c a g g g a c t t c a a g t a g a c g t g g t g g c c g t t c g a c g g g c a c g g g a c c g g g t g g g a g c a c t g g t

4560 65 70 75 80 TFGYGLQCFARYPDHMKQHD EYFP TFGYGLQCFARYPDHMKQHD GEAVAQLAEGAVGVVHLLVV c c t t c g g c t a c g g c c t g c a g t g c t t c g c c c g c t a c c c c g a c c a c a t g a a g c a g c a c g a c t g g a a g c c g a t g c c g g a c g t c a c g a a g c g g g c g a t g g g g c t g g t g t a c t t c g t c g t g c t g a

4620 85 90 95 100 FFKSAMPEGYVQERTIFFKD EYFP C T G C EXFP-R FFKSAMPEGYVQERTIFFKD EELGGHGFAVDLLAGDEELV t c t t c a a g t c c g c c a t g c c c g a a g g c t a c g t c c a g g a g c g c a c c a t c t t c t t c a a g g a c g a g a a g t t c a g g c g g t a c g g g c t t c c g a t g c a g g t c c t c g c g t g g t a g a a g a a g t t c c t g c

4680 105 110 115 120 DGNYKTRAEVKFEGDTLVNR EYFP T G C C G T T G A T G T T C T G EXFP-R DGNYKTRAEVKFEGDTLVNR VAVVLGAGLHLELAVGQHVA a c g g c a a c t a c a a g a c c c g c g c c g a g g t g a a g t t c g a g g g c g a c a c c c t g g t g a a c c g c a t g c c g t t g a t g t t c t g g g c g c g g c t c c a c t t c a a g c t c c c g c t g t g g g a c c a c t t g g c g t

4740 125 130 135 140 IELKGIDFKEDGNILGHKLE EYFP IELKGIDFKEDGNILGHKLE DLQLADVELLVAVDQPVLQL t c g a g c t g a a g g g c a t c g a c t t c a a g g a g g a c g g c a a c a t c c t g g g g c a c a a g c t g g a g t a g c t c g a c t t c c c g t a g c t g a a g t t c c t c c t g c c g t t g t a g g a c c c c g t g t t c g a c c t c a

4800 145 150 155 160 YNYNSHNVYIMADKQKNGIK EYFP YNYNSHNVYIMADKQKNGIK VVVVAVVDIDHGVLLLVADL a c a a c t a c a a c a g c c a c a a c g t c t a t a t c a t g g c c g a c a a g c a g a a g a a c g g c a t c a a g g t g t t g a t g t t g t c g g t g t t g c a g a t a t a g t a c c g g c t g t t c g t c t t c t t g c c g t a g t t c c

4860 165 170 175 180 VNFKIRHNIEDGSVQLADHY EYFP VNFKIRHNIEDGSVQLADHY HVELDAVVDLVAAHLEGVVV t g a a c t t c a a g a t c c g c c a c a a c a t c g a g g a c g g c a g c g t g c a g c t c g c c g a c c a c t a c c a c t t g a a g t t c t a g g c g g t g t t g t a g c t c c t g c c g t c g c a c g t c g a g c g g c t g g t g a t g g

4920 185 190 195 200 QQNTPIGDGPVLLPDNHYLS EYFP QQNTPIGDGPVLLPDNHYLS LLVGGDAVAGHQQGVVVVQA a g c a g a a c a c c c c c a t c g g c g a c g g c c c c g t g c t g c t g c c c g a c a a c c a c t a c c t g a g c t t c g t c t t g t g g g g g t a g c c g c t g c c g g g g c a c g a c g a c g g g c t g t t g g t g a t g g a c t c g a

4980 205 210 215 220 YQSALSKDPNEKRDHMVLLE EYFP C A T G G T C C T G C T G G A G T EGFP-C YQSALSKDPNEKRDHMVLLE VLGGQAFVGVLLAIVHDQQL a c c a g t c c g c c c t g a g c a a a g a c c c c a a c g a g a a g c g c g a t c a c a t g g t c c t g c t g g a g t t g g t c a g g c g g g a c t c g t t t c t g g g g t t g c t c t t c g c g c t a g t g t a c c a g g a c g a c c t c a

SexAI*CsiI 5040 225 230 235 FVTAAGITLGMDELYK (in frame with EYFP) GGPG EYFP T C G T G EGFP-C FVTAAGITLGMDELYKGGPG EHGGGPDSEAHVLQVLTSWT t c g t g a c c g c c g c c g g g a t c a c t c t c g g c a t g g a c g a g c t g t a c a a g g g t g g a c c a g g t g a g c a c t g g c g g c g g c c c t a g t g a g a g c c g t a c c t g c t c g a c a t g t t c c c a c c t g g t c c a c

5100 (in frame with EYFP) GGHQTSLYKKAERET* attR1 GGHQTSLYKKAERET* TSM g t g g a c a t c a a a c a a g t t t g t a c a a a a a a g c t g a a c g a g a a a c g t a a a a t g a t a t a a a t a c a c c t g t a g t t t g t t c a a a c a t g t t t t t t c g a c t t g c t c t t t g c a t t t t a c t a t a t t t a t

5160 attR1 t c a a t a t a t t a a a t t a g a t t t t g c a t a a a a a a c a g a c t a c a t a a t a c t g t a a a a c a c a a c a g t t a t a t a a t t t a a t c t a a a a c g t a t t t t t t g t c t g a t g t a t t a t g a c a t t t t g t g t t g

NotI 5220 -35 attR1 lac UV5 promoter a t a t c c a g t c a c t a t g g c g g c c g c a t t a g g c a c c c c a g g c t t t a c a c t t t a t g c t t c c g g t a t a g g t c a g t g a t a c c g c c g g c g t a a t c c g t g g g g t c c g a a a t g t g a a a t a c g a a g g c c

5280 -10 lac UV5 promoter c t c g t a t a a t g t g t g g a t t t t g a g t t a g g a t c c g t c g a g a t t t t c a g g a g c t a a g g a a g c g a g c a t a t t a c a c a c c t a a a a c t c a a t c c t a g g c a g c t c t a a a a g t c c t c g a t t c c t t c g

5340 1 5 10 15 MEKKITGYTTVDISQWHRK CmR MEKKITGYTTVDISQWHRK t a a a a t g g a g a a a a a a a t c a c t g g a t a t a c c a c c g t t g a t a t a t c c c a a t g g c a t c g t a a a t t t t a c c t c t t t t t t t a g t g a c c t a t a t g g t g g c a a c t a t a t a g g g t t a c c g t a g c a t t

5400 20 25 30 35 EHFEAFQSVAQCTYNQTVQL CmR C T C C G T A A A G T C A G T C A A C G CAT-R EHFEAFQSVAQCTYNQTVQL a g a a c a t t t t g a g g c a t t t c a g t c a g t t g c t c a a t g t a c c t a t a a c c a g a c c g t t c a g c t t c t t g t a a a a c t c c g t a a a g t c a g t c a a c g a g t t a c a t g g a t a t t g g t c t g g c a a g t c g a

5460 40 45 50 55 DITAFLKTVKKNKHKFYPAF CmR DITAFLKTVKKNKHKFYPAF g g a t a t t a c g g c c t t t t t a a a g a c c g t a a a g a a a a a t a a g c a c a a g t t t t a t c c g g c c t t c c t a t a a t g c c g g a a a a a t t t c t g g c a t t t c t t t t t a t t c g t g t t c a a a a t a g g c c g g a a

BspEI 5520 60 65 70 75 IHILARLMNAHPEFRMAMKD CmR IHILARLMNAHPEFRMAMKD t a t t c a c a t t c t t g c c c g c c t g a t g a a t g c t c a t c c g g a a t t c c g t a t g g c a a t g a a a g a a t a a g t g t a a g a a c g g g c g g a c t a c t t a c g a g t a g g c c t t a a g g c a t a c c g t t a c t t t c t

5580 80 85 90 95 GELVIWDSVHPCYTVFHEQT CmR GELVIWDSVHPCYTVFHEQT c g g t g a g c t g g t g a t a t g g g a t a g t g t t c a c c c t t g t t a c a c c g t t t t c c a t g a g c a a a c g c c a c t c g a c c a c t a t a c c c t a t c a c a a g t g g g a a c a a t g t g g c a a a a g g t a c t c g t t t g

5640 100 105 110 115 ETFSSLWSEYHDDFRQFLHI CmR ETFSSLWSEYHDDFRQFLHI t g a a a c g t t t t c a t c g c t c t g g a g t g a a t a c c a c g a c g a t t t c c g g c a g t t t c t a c a c a t a c t t t g c a a a a g t a g c g a g a c c t c a c t t a t g g t g c t g c t a a a g g c c g t c a a a g a t g t g t a

5700 120 125 130 135 YSQDVACYGENLAYFPKGFI CmR YSQDVACYGENLAYFPKGFI a t a t t c g c a a g a t g t g g c g t g t t a c g g t g a a a a c c t g g c c t a t t t c c c t a a a g g g t t t a t t a t a a g c g t t c t a c a c c g c a c a a t g c c a c t t t t g g a c c g g a t a a a g g g a t t t c c c a a a t a

PasI 5760 140 145 150 155 ENMFFVSANPWVSFTSFDLN CmR ENMFFVSANPWVSFTSFDLN t g a g a a t a t g t t t t t c g t c t c a g c c a a t c c c t g g g t g a g t t t c a c c a g t t t t g a t t t a a a a c t c t t a t a c a a a a a g c a g a g t c g g t t a g g g a c c c a c t c a a a g t g g t c a a a a c t a a a t t t

NcoI 5820 160 165 170 175 VANMDNFFAPVFTMGKYYTQ CmR VANMDNFFAPVFTMGKYYTQ c g t g g c c a a t a t g g a c a a c t t c t t c g c c c c c g t t t t c a c c a t g g g c a a a t a t t a t a c g c a g c a c c g g t t a t a c c t g t t g a a g a a g c g g g g g c a a a a g t g g t a c c c g t t t a t a a t a t g c g t

5880 180 185 190 195 GDKVLMPLAIQVHHAVCDGF CmR GDKVLMPLAIQVHHAVCDGF a g g c g a c a a g g t g c t g a t g c c g c t g g c g a t t c a g g t t c a t c a t g c c g t c t g t g a t g g c t t t c c g c t g t t c c a c g a c t a c g g c g a c c g c t a a g t c c a a g t a g t a c g g c a g a c a c t a c c g a a

5940 200 205 210 215 HVGRMLNELQQYCDEWQGGA CmR HVGRMLNELQQYCDEWQGGA c c a t g t c g g c a g a a t g c t t a a t g a a t t a c a a c a g t a c t g c g a t g a g t g g c a g g g c g g g g c g g t a c a g c c g t c t t a c g a a t t a c t t a a t g t t g t c a t g a c g c t a c t c a c c g t c c c g c c c c g

6000 220 * CmR * g t a a a c g c c g c g t g g a t c c g g c t t a c t a a a a g c c a g a t a a c a g t a t g c g t a t t t g c g c g c c a t t t g c g g c g c a c c t a g g c c g a a t g a t t t t c g g t c t a t t g t c a t a c g c a t a a a c g c g c g

BstZ17I 6060 t g a t t t t t g c g g t a t a a g a a t a t a t a c t g a t a t g t a t a c c c g a a g t a t g t c a a a a a g a g g a c t a a a a a c g c c a t a t t c t t a t a t a t g a c t a t a c a t a t g g g c t t c a t a c a g t t t t t c t c c

6120 t a t g c t a t g a a g c a g c g t a t t a c a g t g a c a g t t g a c a g c g a c a g c t a t c a g t t g c t c a a g a t a c g a t a c t t c g t c g c a t a a t g t c a c t g t c a a c t g t c g c t g t c g a t a g t c a a c g a g t t c

6180 g c a t a t a t g a t g t c a a t a t c t c c g g t c t g g t a a g c a c a a c c a t g c a g a a t g a a g c c c g t c c g t a t a t a c t a c a g t t a t a g a g g c c a g a c c a t t c g t g t t g g t a c g t c t t a c t t c g g g c a g

BbvCI 6240 g t c t g c g t g c c g a a c g c t g g a a a g c g g a a a a t c a g g a a g g g a t g g c t g a g g t c g c c c g g t c a g a c g c a c g g c t t g c g a c c t t t c g c c t t t t a g t c c t t c c c t a c c g a c t c c a g c g g g c c a

6300 1 MQFK ccdB t t a t t g a a a t g a a c g g c t c t t t t g c t g a c g a g a a c a g g g g c t g g t g a a a t g c a g t t t a a g a a t a a c t t t a c t t g c c g a g a a a a c g a c t g c t c t t g t c c c c g a c c a c t t t a c g t c a a a t t c

6360 5 10 15 20 VYTYKRESRYRLFVDVQSDI ccdB g t t t a c a c c t a t a a a a g a g a g a g c c g t t a t c g t c t g t t t g t g g a t g t a c a g a g t g a t a t t c a a a t g t g g a t a t t t t c t c t c t c g g c a a t a g c a g a c a a a c a c c t a c a t g t c t c a c t a t a a

BsaBI*SrfI 6420 25 30 35 40 IDTPGRRMVIPLASARLLSD ccdB a t t g a c a c g c c c g g g c g a c g g a t g g t g a t c c c c c t g g c c a g t g c a c g t c t g c t g t c a g a t t a a c t g t g c g g g c c c g c t g c c t a c c a c t a g g g g g a c c g g t c a c g t g c a g a c g a c a g t c t a

6480 45 50 55 60 KVSRELYPVVHIGDESWRMM ccdB G C A T G A T G ccdB-fwd a a a g t c t c c c g t g a a c t t t a c c c g g t g g t g c a t a t c g g g g a t g a a a g c t g g c g c a t g a t g t t t c a g a g g g c a c t t g a a a t g g g c c a c c a c g t a t a g c c c c t a c t t t c g a c c g c g t a c t a c

6540 65 70 75 80 TTDMASVPVSVIGEEVADLS ccdB A C C A C C G A T A T G ccdB-fwd a c c a c c g a t a t g g c c a g t g t g c c g g t c t c c g t t a t c g g g g a a g a a g t g g c t g a t c t c a g c t g g t g g c t a t a c c g g t c a c a c g g c c a g a g g c a a t a g c c c c t t c t t c a c c g a c t a g a g t c g

6600 85 90 95 100 HRENDIKNAINLMFWGI* ccdB c a c c g c g a a a a t g a c a t c a a a a a c g c c a t t a a c c t g a t g t t c t g g g g a a t a t a a a t g t c a g t g g c g c t t t t a c t g t a g t t t t t g c g g t a a t t g g a c t a c a a g a c c c c t t a t a t t t a c a g t

SalI 6660 attR2 g g c t c c c t t a t a c a c a g c c a g t c t g c a g g t c g a c c a t a g t g a c t g g a t a t g t t g t g t t t t c c g a g g g a a t a t g t g t c g g t c a g a c g t c c a g c t g g t a t c a c t g a c c t a t a c a a c a c a a a a

6720 attR2 a c a g t a t t a t g t a g t c t g t t t t t t a t g c a a a a t c t a a t t t a a t a t a t t g a t a t t t a t a t c t g t c a t a a t a c a t c a g a c a a a a a a t a c g t t t t a g a t t a a a t t a t a t a a c t a t a a a t a t a g

6780 attR2 CYC1 terminator a t t t t a c g t t t c t c g t t c a g c t t t c t t g t a c a a a g t g g t t c g a t g t c a t g t a a t t a g t t a t a a a a t g c a a a g a g c a a g t c g a a a g a a c a t g t t t c a c c a a g c t a c a g t a c a t t a a t c a a t

6840 CYC1 terminator C A G T G C G A A T G T A A G T G C G CYC1 t g t c a c g c t t a c a t t c a c g c c c t c c c c c c a c a t c c g c t c t a a c c g a a a a g g a a g g a g t t a a c a g t g c g a a t g t a a g t g c g g g a g g g g g g t g t a g g c g a g a t t g g c t t t t c c t t c c t c a a t

6900 CYC1 terminator g a c a a c c t g a a g t c t a g g t c c c t a t t t a t t t t t t t a t a g t t a t g t t a g t a t t a a g a a c g t c t g t t g g a c t t c a g a t c c a g g g a t a a a t a a a a a a a t a t c a a t a c a a t c a t a a t t c t t g c a

MluI 6960 CYC1 terminator t a t t t a t a t t t c a a a t t t t t c t t t t t t t t c t g t a c a g a c g c g t g t a c g c a t g t a a c a t t a a t a a a t a t a a a g t t t a a a a a g a a a a a a a a g a c a t g t c t g c g c a c a t g c g t a c a t t g t a a t

7020 CYC1 terminator t a c t g a a a a c c t t g c t t g a g a a g g t t t t g g g a c g c t c g a a g g c t t t a a t t t g c g g c c g g t a t g a c t t t t g g a a c g a a c t c t t c c a a a a c c c t g c g a g c t t c c g a a a t t a a a c g c c g g c c a

7080 T7 promoter M13 fwd G G G A T A T C A C T C A G C A T A A T T7 T G A C C G G C A G C A A A A T G T M13 Forward G C A A A A T G T T G C M13/pUC Forward a c c c a a t t c g c c c t a t a g t g a g t c g t a t t a c g c g c g c t c a c t g g c c g t c g t t t t a c a a c g t g g g t t a a g c g g g a t a t c a c t c a g c a t a a t g c g c g c g a g t g a c c g g c a g c a a a a t g t t g c

7140 A G C A C T G A C C C M13/pUC Forward t c g t g a c t g g g a a a a c c c t g g c g t t a c c c a a c t t a a t c g c c t t g c a g c a c a t c c c c c t t t a g c a c t g a c c c t t t t g g g a c c g c a a t g g g t t g a a t t a g c g g a a c g t c g t g t a g g g g g a a a

7200 c g c c a g c t g g c g t a a t a g c g a a g a g g c c c g c a c c g a t c g c c c t t c c c a a c a g t t g c g c a g g c g g t c g a c c g c a t t a t c g c t t c t c c g g g c g t g g c t a g c g g g a a g g g t t g t c a a c g c g t c

7260 f1 ori c c t g a a t g g c g a a t g g c g c g a c g c g c c c t g t a g c g g c g c a t t a a g c g c g g c g g g t g t g g t g g a c t t a c c g c t t a c c g c g c t g c g c g g g a c a t c g c c g c g t a a t t c g c g c c g c c c a c a c c a

7320 f1 ori G A G G A A A G C G A A A F1ori-R g g t t a c g c g c a g c g t g a c c g c t a c a c t t g c c a g c g c c c t a g c g c c c g c t c c t t t c g c t t t c c a a t g c g c g t c g c a c t g g c g a t g t g a a c g g t c g c g g g a t c g c g g g c g a g g a a a g c g a a a

NgoMIVNaeI 7380 f1 ori G A A G G G A F1ori-R c t t c c c t t c c t t t c t c g c c a c g t t c g c c g g c t t t c c c c g t c a a g c t c t a a a t c g g g g g c t g a a g g g a a g g a a a g a g c g g t g c a a g c g g c c g a a a g g g g c a g t t c g a g a t t t a g c c c c c g a

7440 f1 ori c c c t t t a g g g t t c c g a t t t a g t g c t t t a c g g c a c c t c g a c c c c a a a a a a c t t g a t t a g g g g g g a a a t c c c a a g g c t a a a t c a c g a a a t g c c g t g g a g c t g g g g t t t t t t g a a c t a a t c c c

DraIII 7500 f1 ori t g a t g g t t c a c g t a g t g g g c c a t c g c c c t g a t a g a c g g t t t t t c g c c c t t t g a c g t t g g a a c t a c c a a g t g c a t c a c c c g g t a g c g g g a c t a t c t g c c a a a a a g c g g g a a a c t g c a a c c t

7560 f1 ori G T G G A C T C T T G T T C C A A A C T G G F1ori-F g t c c a c g t t c t t t a a t a g t g g a c t c t t g t t c c a a a c t g g a a c a a c a c t c a a c c c t a t c t c c a g g t g c a a g a a a t t a t c a c c t g a g a a c a a g g t t t g a c c t t g t t g t g a g t t g g g a t a g a g

7620 f1 ori g g t c t a t t c t t t t g a t t t a t a a g g g a t t t t g c c g a t t t c g g c c t a t t g g t t a a a a a a t g a c c a g a t a a g a a a a c t a a a t a t t c c c t a a a a c g g c t a a a g c c g g a t a a c c a a t t t t t t a c t

7680 f1 ori g c t g a t t t a a c a a a a a t t t a a c g c g a a t t t t a a c a a a a t a t t a a c g t t t a c a a t t t c c t g c g a c t a a a t t g t t t t t a a a t t g c g c t t a a a a t t g t t t t a t a a t t g c a a a t g t t a a a g g a c

7740 a t g c g g t a t t t t c t c c t t a c g c a t c t g t g c g g t a t t t c a c a c c g c a t a t c g a c g g t c g a g t a c g c c a t a a a a g a g g a a t g c g t a g a c a c g c c a t a a a g t g t g g c g t a t a g c t g c c a g c t c

7800 g a g a a c t t c t a g t a t a t c c a c a t a c c t a a t a t t a t t g c c t t a t t a a a a a t g g a a t c c c a a c t c t t g a a g a t c a t a t a g g t g t a t g g a t t a t a a t a a c g g a a t a a t t t t t a c c t t a g g g t t

7860 c a a t t a c a t c a a a a t c c a c a t t c t c t t c a a a a t c a a t t g t c c t g t a c t t c c t t g t t c a t g g t t a a t g t a g t t t t a g g t g t a a g a g a a g t t t t a g t t a a c a g g a c a t g a a g g a a c a a g t a c

7920 t g t g t t c a a a a a c g t t a t a t t t a t a g g a t a a t t a t a c t c t a t t t c t c a a c a a g t a a t t g g a c a c a a g t t t t t g c a a t a t a a a t a t c c t a t t a a t a t g a g a t a a a g a g t t g t t c a t t a a c c

HpaIKasINarISfoIPluTI 7980 LEU2 promoter t t g t t t g g c c g a g c g g t c t a a g g c g c c t g a t t c a a g a a a t a t c t t g a c c g c a g t t a a c t g a a c a a a c c g g c t c g c c a g a t t c c g c g g a c t a a g t t c t t t a t a g a a c t g g c g t c a a t t g a c

8040 LEU2 promoter t g g g a a t a c t c a g g t a t c g t a a g a t g c a a g a g t t c g a a t c t c t t a g c a a c c a t t a t t t t t a c c c t t a t g a g t c c a t a g c a t t c t a c g t t c t c a a g c t t a g a g a a t c g t t g g t a a t a a a a a

8100 LEU2 promoter t t c c t c a a c a t a a c g a g a a c a c a c a g g g g c g c t a t c g c a c a g a a t c a a a t t c g a t g a c t g a a g g a g t t g t a t t g c t c t t g t g t g t c c c c g c g a t a g c g t g t c t t a g t t t a a g c t a c t g a c

8160 LEU2 promoter g a a a t t t t t t g t t a a t t t c a g a g g t c g c c t g a c g c a t a t a c c t t t t t c a a c t g a a a a a t t c t t t a a a a a a c a a t t a a a g t c t c c a g c g g a c t g c g t a t a t g g a a a a a g t t g a c t t t t t a a

8220 LEU2 promoter g g g a g a a a a a g g a a a g g t g a g a g g c c g g a a c c g g c t t t t c a t a t a g a a t a g a g a a g c g t t c c c t c t t t t t c c t t t c c a c t c t c c g g c c t t g g c c g a a a a g t a t a t c t t a t c t c t t c g c a a

8280 LEU2 promoter c a t g a c t a a a t g c t t g c a t c a c a a t a c t t g a a g t t g a c a a t a t t a t t t a a g g a c c t a t t g g t a c t g a t t t a c g a a c g t a g t g t t a t g a a c t t c a a c t g t t a t a a t a a a t t c c t g g a t a a c

8340 LEU2 promoter t t t t t t c c a a t a g g t g g t t a g c a a t c g t c t t a c t t t c t a a c t t t t c t t a c c t t t t a c a t t a a a a a a g g t t a t c c a c c a a t c g t t a g c a g a a t g a a a g a t t g a a a a g a a t g g a a a a t g t a a

8400 1 MSA LEU2 promoter LEU2 MSAPMSA t c a g c a a t a t a t a t a t a t a t t t c a a g g a t a t a c c a t t c t a a t g t c t g c c c c t a t g t c t g c a g t c g t t a t a t a t a t a t a t a a a g t t c c t a t a t g g t a a g a t t a c a g a c g g g g a t a c a g a c g

BstEII 8460 5 10 15 20 PKKIVVLPGDHVGQEITAEA LEU2 PKKIVVLPGDHVGQEITAEA c c c t a a g a a g a t c g t c g t t t t g c c a g g t g a c c a c g t t g g t c a a g a a a t c a c a g c c g a a g c g g g a t t c t t c t a g c a g c a a a a c g g t c c a c t g g t g c a a c c a g t t c t t t a g t g t c g g c t t c g

8520 25 30 35 40 IKVLKAISDVRSNVKFDFEN LEU2 IKVLKAISDVRSNVKFDFEN c a t t a a g g t t c t t a a a g c t a t t t c t g a t g t t c g t t c c a a t g t c a a g t t c g a t t t c g a a a a g t a a t t c c a a g a a t t t c g a t a a a g a c t a c a a g c a a g g t t a c a g t t c a a g c t a a a g c t t t t

BspDIClaI 8580 45 50 55 60 HLIGGAAIDATGVPLPDEAL LEU2 HLIGGAAIDATGVPLPDEAL t c a t t t a a t t g g t g g t g c t g c t a t c g a t g c t a c a g g t g t c c c a c t t c c a g a t g a g g c g c t a g t a a a t t a a c c a c c a c g a c g a t a g c t a c g a t g t c c a c a g g g t g a a g g t c t a c t c c g c g a

8640 65 70 75 80 EASKKVDAVLLGAVAGPKWG LEU2 EASKKVDAVLLGAVAGPKWG g g a a g c c t c c a a g a a g g t t g a t g c c g t t t t g t t a g g t g c t g t g g c t g g t c c t a a a t g g g g c c t t c g g a g g t t c t t c c a a c t a c g g c a a a a c a a t c c a c g a c a c c g a c c a g g a t t t a c c c c

AgeI 8700 85 90 95 100 TGSVRPEQGLLKIRKELQLY LEU2 TGSVRPEQGLLKIRKELQLY t a c c g g t a g t g t t a g a c c t g a a c a a g g t t t a c t a a a a a t c c g t a a a g a a c t t c a a t t g t a a t g g c c a t c a c a a t c t g g a c t t g t t c c a a a t g a t t t t t a g g c a t t t c t t g a a g t t a a c a t

AflII 8760 105 110 115 120 ANLRPCNFASDSLLDLSPIK LEU2 ANLRPCNFASDSLLDLSPIK c g c c a a c t t a a g a c c a t g t a a c t t t g c a t c c g a c t c t c t t t t a g a c t t a t c t c c a a t c a a g c g g t t g a a t t c t g g t a c a t t g a a a c g t a g g c t g a g a g a a a a t c t g a a t a g a g g t t a g t t

8820 125 130 135 140 PQFAKGTDFVVVRELVGGIY LEU2 PQFAKGTDFVVVRELVGGIY g c c a c a a t t t g c t a a a g g t a c t g a c t t c g t t g t t g t c a g a g a a t t a g t g g g a g g t a t t t a c g g t g t t a a a c g a t t t c c a t g a c t g a a g c a a c a a c a g t c t c t t a a t c a c c c t c c a t a a a t

8880 145 150 155 160 FGKRKEDDGDGVAWDSEQYT LEU2 FGKRKEDDGDGVAWDSEQYT c t t t g g t a a g a g a a a g g a a g a c g a t g g t g a t g g t g t c g c t t g g g a t a g t g a a c a a t a c a c g a a a c c a t t c t c t t t c c t t c t g c t a c c a c t a c c a c a g c g a a c c c t a t c a c t t g t t a t g t g

8940 165 170 175 180 VPEVQRITRMAAFMALQHEP LEU2 VPEVQRITRMAAFMALQHEP c g t t c c a g a a g t g c a a a g a a t c a c a a g a a t g g c c g c t t t c a t g g c c c t a c a a c a t g a g c c g c a a g g t c t t c a c g t t t c t t a g t g t t c t t a c c g g c g a a a g t a c c g g g a t g t t g t a c t c g g

9000 185 190 195 200 PLPIWSLDKANLLASSRLWR LEU2 PLPIWSLDKANLLASSRLWR a c c a t t g c c t a t t t g g t c c t t g g a t a a a g c t a a t c t t t t g g c c t c t t c a a g a t t a t g g a g t g g t a a c g g a t a a a c c a g g a a c c t a t t t c g a t t a g a a a a c c g g a g a a g t t c t a a t a c c t c

9060 205 210 215 220 KTVEETIKNEFPTLKVQHQL LEU2 KTVEETIKNEFPTLKVQHQL a a a a a c t g t g g a g g a a a c c a t c a a g a a c g a a t t c c c t a c a t t g a a g g t t c a a c a t c a a t t t t t t t g a c a c c t c c t t t g g t a g t t c t t g c t t a a g g g a t g t a a c t t c c a a g t t g t a g t t a a

9120 225 230 235 240 IDSAAMILVKNPTHLNGIII LEU2 IDSAAMILVKNPTHLNGIII g a t t g a t t c t g c c g c c a t g a t c c t a g t t a a g a a c c c a a c c c a c c t a a a t g g t a t t a t a a t c t a a c t a a g a c g g c g g t a c t a g g a t c a a t t c t t g g g t t g g g t g g a t t t a c c a t a a t a t t a

EcoRV 9180 245 250 255 260 TSNMFGDIISDEASVIPGSL LEU2 TSNMFGDIISDEASVIPGSL c a c c a g c a a c a t g t t t g g t g a t a t c a t c t c c g a t g a a g c c t c c g t t a t c c c a g g t t c c t t g t g g t c g t t g t a c a a a c c a c t a t a g t a g a g g c t a c t t c g g a g g c a a t a g g g t c c a a g g a a

9240 265 270 275 280 GLLPSASLASLPDKNTAFGL LEU2 GLLPSASLASLPDKNTAFGL g g g t t t g t t g c c a t c t g c g t c c t t g g c c t c t t t g c c a g a c a a g a a c a c c g c a t t t g g t t t c c c a a a c a a c g g t a g a c g c a g g a a c c g g a g a a a c g g t c t g t t c t t g t g g c g t a a a c c a a a

9300 285 290 295 300 YEPCHGSAPDLPKNKVDPIA LEU2 YEPCHGSAPDLPKNKVDPIA g t a c g a a c c a t g c c a c g g t t c t g c t c c a g a t t t g c c a a a g a a t a a g g t t g a c c c t a t c g c c a t g c t t g g t a c g g t g c c a a g a c g a g g t c t a a a c g g t t t c t t a t t c c a a c t g g g a t a g c g

9360 305 310 315 320 TILSAAMMLKLSLNLPEEGK LEU2 TILSAAMMLKLSLNLPEEGK c a c t a t c t t g t c t g c t g c a a t g a t g t t g a a a t t g t c a t t g a a c t t g c c t g a a g a a g g t a a g t g a t a g a a c a g a c g a c g t t a c t a c a a c t t t a a c a g t a a c t t g a a c g g a c t t c t t c c a t t

9420 325 330 335 340 AIEDAVKKVLDAGIRTGDLG LEU2 AIEDAVKKVLDAGIRTGDLG g g c c a t t g a a g a t g c a g t t a a a a a g g t t t t g g a t g c a g g t a t c a g a a c t g g t g a t t t a g g c c g g t a a c t t c t a c g t c a a t t t t t c c a a a a c c t a c g t c c a t a g t c t t g a c c a c t a a a t c c

9480 345 350 355 360 GSNSTTEVGDAVAEEVKKIL LEU2 GSNSTTEVGDAVAEEVKKIL t g g t t c c a a c a g t a c c a c c g a a g t c g g t g a t g c t g t c g c c g a a g a a g t t a a g a a a a t c c t a c c a a g g t t g t c a t g g t g g c t t c a g c c a c t a c g a c a g c g g c t t c t t c a a t t c t t t t a g g a

9540 365 A* LEU2 A* t g c t t a a a a a g a t t c t c t t t t t t t a t g a t a t t t g t a c a t a a a c t t t a t a a a t g a a a t t c a a c g a a t t t t t c t a a g a g a a a a a a a t a c t a t a a a c a t g t a t t t g a a a t a t t t a c t t t a a g t

9600 t a a t a g a a a c g a c a c g a a a t t a c a a a a t g g a a t a t g t t c a t a g g g t a g a c g a a a c t a t a t a t t a t c t t t g c t g t g c t t t a a t g t t t t a c c t t a t a c a a g t a t c c c a t c t g c t t t g a t a t a

9660 a c g c a a t c t a c a t a c a t t t a t c a a g a a g g a g a a a a a g g a g g a t a g t a a a g g a a t a c a g g t t g c g t t a g a t g t a t g t a a a t a g t t c t t c c t c t t t t t c c t c c t a t c a t t t c c t t a t g t c c a

9720 a a g c a a a t t g a t a c t a a t g g c t c a a c g t g a t a a g g a a a a a g a a t t g c a c t t t a a c a t t a a t t c g t t t a a c t a t g a t t a c c g a g t t g c a c t a t t c c t t t t t c t t a a c g t g a a a t t g t a a t t

9780 t a t t g a c a a g g a g g a g g g c a c c a c a c a a a a a g t t a g g t g t a a c a g a a a a t c a t g a a a c t a a t a a c t g t t c c t c c t c c c g t g g t g t g t t t t t c a a t c c a c a t t g t c t t t t a g t a c t t t g a t

9840 c g a t t c c t a a t t t g a t a t t g g a g g a t t t t c t c t a a a a a a a a a a a a a t a c a a c a a a t a a a a g c t a a g g a t t a a a c t a t a a c c t c c t a a a a g a g a t t t t t t t t t t t t t a t g t t g t t t a t t t t

9900 a a c a c t c a a t g a c c t g a c c a t t t g a t g g a g t t t a a g t c a a t a c c t t c t t g a a g c a t t t c c t t g t g a g t t a c t g g a c t g g t a a a c t a c c t c a a a t t c a g t t a t g g a a g a a c t t c g t a a a g g

Tth111IPflFI 9960 c a t a a t g g t g a a a g t t c c c t c a a g a a t t t t a c t c t g t c a g a a a c g g c c t t a c g a c g t a g t g t a t t a c c a c t t t c a a g g g a g t t c t t a a a a t g a g a c a g t c t t t g c c g g a a t g c t g c a t c a

10,020 A T G T T A G A C G A G A C T A C G G C pRS-marker c g a t a t g g t g c a c t c t c a g t a c a a t c t g c t c t g a t g c c g c a t a g t t a a g c c a g c c c c g a c g c t a t a c c a c g t g a g a g t c a t g t t a g a c g a g a c t a c g g c g t a t c a a t t c g g t c g g g g c t g

10,080 a c c c g c c a a c a c c c g c t g a c g c g c c c t g a c g g g c t t g t c t g c t c c c g g c a t c c g c t t a c a t g g g c g g t t g t g g g c g a c t g c g c g g g a c t g c c c g a a c a g a c g a g g g c c g t a g g c g a a t g t

10,140 C C G G G A G C T G C A T G T G T C A G A G G pGEX 3' g a c a a g c t g t g a c c g t c t c c g g g a g c t g c a t g t g t c a g a g g t t t t c a c c g t c a t c a c c g a c t g t t c g a c a c t g g c a g a g g c c c t c g a c g t a c a c a g t c t c c a a a a g t g g c a g t a g t g g c t

3ʹ 5ʹ 10,149 a a c g c g c g a t t g c g c g c t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.