Addgene PTPRJ-bio-His - Sequence Analyzer Skip to main content
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Sequence Analyzer: PTPRJ-bio-His


Map View

AscI - BssHII (9542) FseI (9209) StuI (9175) BstEII (8649) XcmI (7678) EagI - NotI (6620) PmeI (6613) pMT2-F (6572 .. 6591) BsmBI - Esp3I (6386) BlpI (6303) PaeR7I - XhoI (6263) SacI (6070) Eco53kI (6068) LNCX (6067 .. 6091) CMV-F (6021 .. 6041) CMV promoter SnaBI (5842) M13 Reverse (5200 .. 5216) lac operator M13/pUC Reverse (5181 .. 5203) CAP binding site BspQI - SapI (4987) L4440 (4968 .. 4985) KflI (371) EcoRI (550) XbaI (666) PacI (683) rbglobpA-R (767 .. 786) BspDI * - ClaI * (835) BstAPI (1066) HpaI (1274) MluI (2090) pBRforEco (3004 .. 3022) Amp-R (3413 .. 3432) PvuI (3613) FspI (3759) pBR322ori-F (4715 .. 4734) PTPRJ-bio-His 9548 bp

Sequence View

60 STSITAYKSEGESAEFSFPL g t c g a c c t c c a t c a c g g c c t a t a a g a g t g a g g g g g a g t c a g c g g a g t t c t c c t t c c c a c t c a g c t g g a g g t a g t g c c g g a t a t t c t c a c t c c c c c t c a g t c g c c t c a a g a g g a a g g g t g a

120 NLGEESLQGELRWKAEKAPS c a a c c t t g g a g a g g a a a g c c t g c a g g g a g a g t t g a g a t g g a a g g c a g a g a a g g c t c c t t c g t t g g a a c c t c t c c t t t c g g a c g t c c c t c t c a a c t c t a c c t t c c g t c t c t t c c g a g g a a g

180 SQSWITFSLKNQKVSVQKST t t c c c a g t c c t g g a t c a c c t t c t c c c t a a a g a a c c a a a a g g t g t c t g t g c a g a a g t c t a c a a g g g t c a g g a c c t a g t g g a a g a g g g a t t t c t t g g t t t t c c a c a g a c a c g t c t t c a g a t g

240 SNPKFQLSETLPLTLQIPQV t a g c a a c c c c a a g t t c c a g c t g t c c g a a a c g c t c c c a c t c a c c c t t c a g a t a c c c c a g g t a t c g t t g g g g t t c a a g g t c g a c a g g c t t t g c g a g g g t g a g t g g g a a g t c t a t g g g g t c c a

300 SLQFAGSGNLTLTLDRGILY c t c c c t t c a g t t t g c t g g t t c t g g c a a c c t g a c c c t g a c t c t g g a c a g a g g g a t a c t g t a g a g g g a a g t c a a a c g a c c a a g a c c g t t g g a c t g g g a c t g a g a c c t g t c t c c c t a t g a c a t

360 QEVNLVVMKVTQPDSNTLTC t c a g g a a g t g a a c c t g g t g g t g a t g a a a g t g a c t c a g c c c g a c a g c a a c a c t t t g a c c t g a g t c c t t c a c t t g g a c c a c c a c t a c t t t c a c t g a g t c g g g c t g t c g t t g t g a a a c t g g a c

KflI 420 EVMGPTSPKMRLILKQENQE t g a g g t g a t g g g a c c c a c c t c a c c c a a g a t g a g a c t g a t c t t g a a g c a g g a g a a t c a g g a a c t c c a c t a c c c t g g g t g g a g t g g g t t c t a c t c t g a c t a g a a c t t c g t c c t c t t a g t c c t

480 ARVSRQEKVIQVQAPEAGVW g g c c a g g g t c t c c a g g c a g g a g a a a g t g a t t c a a g t g c a g g c c c c t g a a g c a g g g g t g t g c c g g t c c c a g a g g t c c g t c c t c t t t c a c t a a g t t c a c g t c c g g g g a c t t c g t c c c c a c a c

540 QCLLSEGEEVKMDSKIQVLS g c a a t g t c t a c t g a g t g a a g g t g a a g a g g t c a a g a t g g a c t c c a a g a t c c a g g t t t t a t c c g t t a c a g a t g a c t c a c t t c c a c t t c t c c a g t t c t a c c t g a g g t t c t a g g t c c a a a a t a g

EcoRI 600 KGLNSGSLHHILDAQKMLWN c a a a g g g t t g a a t t c c g g c a g c c t g c a c c a c a t c c t g g a c g c c c a g a a g a t g c t g t g g a a g t t t c c c a a c t t a a g g c c g t c g g a c g t g g t g t a g g a c c t g c g g g t c t t c t a c g a c a c c t t

660 1 5 HHHHHH 6xHis HRDRNLPPLAPLGPHHHHHH c c a c a g g g a c a g g a a c c t g c c t c c t c t g g c c c c t c t g g g c c c c c a c c a c c a t c a c c a c c a g g t g t c c c t g t c c t t g g a c g g a g g a g a c c g g g g a g a c c c g g g g g t g g t g g t a g t g g t g g t

PacIXbaI 720 * 6xHis β- globin poly(A) signal * t t g a g t c t a g a c a c g t g a t t a a t t a a g g a t c c c c c g a c c t c g a c c t c t g g c t a a t a a a g g a a c t c a g a t c t g t g c a c t a a t t a a t t c c t a g g g g g c t g g a g c t g g a g a c c g a t t a t t t c c

780 β- globin poly(A) signal G T G A G C C T T C C T G T rbglobpA-R a a a t t t a t t t t c a t t g c a a t a g t g t g t t g g a a t t t t t t g t g t c t c t c a c t c g g a a g g a c a t t t a a a t a a a a g t a a c g t t a t c a c a c a a c c t t a a a a a a c a c a g a g a g t g a g c c t t c c t g t

BspDI*ClaI* 840 A T A C C C rbglobpA-R t a t g g g a g g g c a a a t c a t t t g g t c g a g a t c c c t c g g a g a t c t c t a g c t a g a g g a t c g a t c a t a c c c t c c c g t t t a g t a a a c c a g c t c t a g g g a g c c t c t a g a g a t c g a t c t c c t a g c t a g

900 c c c g c c c c g g a c g a a c t a a a c c t g a c t a c g a c a t c t c t g c c c c t t c t t c g c g g g g c a g t g g g g c g g g g c c t g c t t g a t t t g g a c t g a t g c t g t a g a g a c g g g g a a g a a g c g c c c c g t c a c

960 c a t g t a a t c c c t t c a g t t g g t t g g t a c a a c t t g c c a a c t g g g c c c t g t t c c a c a t g t g a c g t a c a t t a g g g a a g t c a a c c a a c c a t g t t g a a c g g t t g a c c c g g g a c a a g g t g t a c a c t g

1020 a c g g g g g g g g a c c a a a c a c a a a g g g g t t c t c t g a c t g t a g t t g a c a t c c t t a t a a a t g g a t g c c c c c c c c t g g t t t g t g t t t c c c c a a g a g a c t g a c a t c a a c t g t a g g a a t a t t t a c c t

BstAPI 1080 t g t g c a c a t t t g c c a a c a c t g a g t g g c t t t c a t c c t g g a g c a g a c t t t g c a g t c t g t g g a a c a c g t g t a a a c g g t t g t g a c t c a c c g a a a g t a g g a c c t c g t c t g a a a c g t c a g a c a c c t

1140 c t g c a a c a c a a c a t t g c c t t t a t g t g t a a c t c t t g g c t g a a g c t c t t a c a c c a a t g c t g g g a c g t t g t g t t g t a a c g g a a a t a c a c a t t g a g a a c c g a c t t c g a g a a t g t g g t t a c g a c c

1200 g g g a c a t g t a c c t c c c a g g g g c c c a g g a a g a c t a c g g g a g g c t a c a c c a a c g t c a a t c a g c c c t g t a c a t g g a g g g t c c c c g g g t c c t t c t g a t g c c c t c c g a t g t g g t t g c a g t t a g t c

1260 a g g g g c c t g t g t a g c t a c c g a t a a g c g g a c c c t c a a g a g g g c a t t a g c a a t a g t g t t t a t t c c c c g g a c a c a t c g a t g g c t a t t c g c c t g g g a g t t c t c c c g t a a t c g t t a t c a c a a a t a

HpaI 1320 a a g g c c c c c t t g t t a a c c c t a a a c g g g t a g c a t a t g c t t c c c g g g t a g t a g t a t a t a c t a t t c c g g g g g a a c a a t t g g g a t t t g c c c a t c g t a t a c g a a g g g c c c a t c a t c a t a t a t g a t

1380 t c c a g a c t a a c c c t a a t t c a a t a g c a t a t g t t a c c c a a c g g g a a g c a t a t g c t a t c g a a t a g g t c t g a t t g g g a t t a a g t t a t c g t a t a c a a t g g g t t g c c c t t c g t a t a c g a t a g c t t a

1440 t a g g g t t a g t a a a a g g g t c c t a a g g a a c a g c g a t a t c t c c c a c c c c a t g a g c t g t c a c g g a t c c c a a t c a t t t t c c c a g g a t t c c t t g t c g c t a t a g a g g g t g g g g t a c t c g a c a g t g c c

1500 t t t t a t t t a c a t g g g g t c a g g a t t c c a c g a g g g t a g t g a a c c a t t t t a g t c a c a a g g g c a a a a a t a a a t g t a c c c c a g t c c t a a g g t g c t c c c a t c a c t t g g t a a a a t c a g t g t t c c c g t

1560 g t g g c t g a a g a t c a a g g a g c g g g c a g t g a a c t c t c c t g a a t c t t c g c c t g c t t c t t c a t t c a c c g a c t t c t a g t t c c t c g c c c g t c a c t t g a g a g g a c t t a g a a g c g g a c g a a g a a g t a a

1620 c t c c t t c g t t t a g c t a a t a g a a t a a c t g c t g a g t t g t g a a c a g t a a g g t g t a t g t g a g g t g a g g a a g c a a a t c g a t t a t c t t a t t g a c g a c t c a a c a c t t g t c a t t c c a c a t a c a c t c c a

1680 g c t c g a a a a c a a g g t t t c a g g t g a c g c c c c c a g a a t a a a a t t t g g a c g g g g g g t t c a g t g c g a g c t t t t g t t c c a a a g t c c a c t g c g g g g g t c t t a t t t t a a a c c t g c c c c c c a a g t c a c

1740 g t g g c a t t g t g c t a t g a c a c c a a t a t a a c c c t c a c a a a c c c c t t g g g c a a t a a a t a c t a g c a c c g t a a c a c g a t a c t g t g g t t a t a t t g g g a g t g t t t g g g g a a c c c g t t a t t t a t g a t c

1800 t g t a g g a a t g a a a c a t t c t g a a t a t c t t t a a c a a t a g a a a t c c a t g g g g t g g g g a c a a g c a c a t c c t t a c t t t g t a a g a c t t a t a g a a a t t g t t a t c t t t a g g t a c c c c a c c c c t g t t c g

1860 c g t a a a g a c t g g a t g t c c a t c t c a c a c g a a t t t a t g g c t a t g g g c a a c a c a t a a t c c t a g g c a t t t c t g a c c t a c a g g t a g a g t g t g c t t a a a t a c c g a t a c c c g t t g t g t a t t a g g a t c

1920 t g c a a t a t g a t a c t g g g g t t a t t a a g a t g t g t c c c a g g c a g g g a c c a a g a c a g g t g a a c c a c g t t a t a c t a t g a c c c c a a t a a t t c t a c a c a g g g t c c g t c c c t g g t t c t g t c c a c t t g g

1980 a t g t t g t t a c a c t c t a t t t g t a a c a a g g g g a a a g a g a g t g g a c g c c g a c a g c a g c g g a c t t a c a a c a a t g t g a g a t a a a c a t t g t t c c c c t t t c t c t c a c c t g c g g c t g t c g t c g c c t g a

2040 c c a c t g g t t g t c t c t a a c a c c c c c g a a a a t t a a a c g g g g c t c c a c g c c a a t g g g g c c c a t g g t g a c c a a c a g a g a t t g t g g g g g c t t t t a a t t t g c c c c g a g g t g c g g t t a c c c c g g g t a

MluI 2100 a a a c a a a g a c a a g t g g c c a c t c t t t t t t t t g a a a t t g t g g a g t g g g g g c a c g c g t c a g c c t t t g t t t c t g t t c a c c g g t g a g a a a a a a a a c t t t a a c a c c t c a c c c c c g t g c g c a g t c g g

2160 c c c a c a c g c c g c c c t g c g g t t t t g g a c t g t a a a a t a a g g g t g t a a t a a c t t g g c t g a t t g g g g t g t g c g g c g g g a c g c c a a a a c c t g a c a t t t t a t t c c c a c a t t a t t g a a c c g a c t a a c

2220 t a a c c c c g c t a a c c a c t g c g g t c a a a c c a c t t g c c c a c a a a a c c a c t a a t g g c a c c c c g g a t t g g g g c g a t t g g t g a c g c c a g t t t g g t g a a c g g g t g t t t t g g t g a t t a c c g t g g g g c c

2280 g g a a t a c c t g c a t a a g t a g g t g g g c g g g c c a a g a t a g g g g c g c g a t t g c t g c g a t c t g g a c c t t a t g g a c g t a t t c a t c c a c c c g c c c g g t t c t a t c c c c g c g c t a a c g a c g c t a g a c c t

2340 g g a c a a a t t a c a c a c a c t t g c g c c t g a g c g c c a a g c a c a g g g t t g t t g g t c c t c a t a t t c c c t g t t t a a t g t g t g t g a a c g c g g a c t c g c g g t t c g t g t c c c a a c a a c c a g g a g t a t a a g

2400 a c g a g g t c g c t g a g a g c a c g g t g g g c t a a t g t t g c c a t g g g t a g c a t a t a c t a c c c a a a t t g c t c c a g c g a c t c t c g t g c c a c c c g a t t a c a a c g g t a c c c a t c g t a t a t g a t g g g t t t a

2460 a t c t g g a t a g c a t a t g c t a t c c t a a t c t a t a t c t g g g t a g c a t a g g c t a t c c t a a t c t a t t a g a c c t a t c g t a t a c g a t a g g a t t a g a t a t a g a c c c a t c g t a t c c g a t a g g a t t a g a t a

2520 a t c t g g g t a g c a t a t g c t a t c c t a a t c t a t a t c t g g g t a g t a t a t g c t a t c c t a a t t t a t t a g a c c c a t c g t a t a c g a t a g g a t t a g a t a t a g a c c c a t c a t a t a c g a t a g g a t t a a a t a

2580 a t c t g g g t a g c a t a g g c t a t c c t a a t c t a t a t c t g g g t a g c a t a t g c t a t c c t a a t c t a t t a g a c c c a t c g t a t c c g a t a g g a t t a g a t a t a g a c c c a t c g t a t a c g a t a g g a t t a g a t a

2640 a t c t g g g t a g t a t a t g c t a t c c t a a t c t g t a t c c g g g t a g c a t a t g c t a t c c t a a t a g a g t a g a c c c a t c a t a t a c g a t a g g a t t a g a c a t a g g c c c a t c g t a t a c g a t a g g a t t a t c t c

2700 a t t a g g g t a g t a t a t g c t a t c c t a a t t t a t a t c t g g g t a g c a t a t a c t a c c c a a a t a t c t t a a t c c c a t c a t a t a c g a t a g g a t t a a a t a t a g a c c c a t c g t a t a t g a t g g g t t t a t a g a

2760 g g a t a g c a t a t g c t a t c c t a a t c t a t a t c t g g g t a g c a t a t g c t a t c c t a a t c t a t a t c t c c t a t c g t a t a c g a t a g g a t t a g a t a t a g a c c c a t c g t a t a c g a t a g g a t t a g a t a t a g a

2820 g g g t a g c a t a g g c t a t c c t a a t c t a t a t c t g g g t a g c a t a t g c t a t c c t a a t c t a t a t c t c c c a t c g t a t c c g a t a g g a t t a g a t a t a g a c c c a t c g t a t a c g a t a g g a t t a g a t a t a g a

2880 g g g t a g t a t a t g c t a t c c t a a t t t a t a t c t g g g t a g c a t a g g c t a t c c t a a t c t a t a t c t c c c a t c a t a t a c g a t a g g a t t a a a t a t a g a c c c a t c g t a t c c g a t a g g a t t a g a t a t a g a

2940 g g g t a g c a t a t g c t a t c c t a a t c t a t a t c t g g g t a g t a t a t g c t a t c c t a a t c t g t a t c c c c c a t c g t a t a c g a t a g g a t t a g a t a t a g a c c c a t c a t a t a c g a t a g g a t t a g a c a t a g g

3000 g g g t a g c a t a t g c t a t c c t c a t g a t a a g c t g t c a a a c a t g a g a a t t t t c t t g a a g a c g a a c c c a t c g t a t a c g a t a g g a g t a c t a t t c g a c a g t t t g t a c t c t t a a a a g a a c t t c t g c t t

3060 C G G A G C A C T A T G C G G A T A A pBRforEco a g g g c c t c g t g a t a c g c c t a t t t t t a t a g g t t a a t g t c a t g a t a a t a a t g g t t t c t t a g a t c c c g g a g c a c t a t g c g g a t a a a a a t a t c c a a t t a c a g t a c t a t t a t t a c c a a a g a a t c t

3120 AmpR promoter c g t c a g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a g c a g t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t

3180 AmpR promoter t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a

3240 1 5 10 15 signal sequence MSIQHFRVALIPFFA AmpR promoter AmpR MSIQHFRVALIPFFA g a a a a a g g a a g a g t a t g a g t a t t c a a c a t t t c c g t g t c g c c c t t a t t c c c t t t t t t g c g g c t t t t t c c t t c t c a t a c t c a t a a g t t g t a a a g g c a c a g c g g g a a t a a g g g a a a a a a c g c c

3300 20 signal sequence AFCLPVFA 25 30 35 HPETLVKVKDAE AmpR AFCLPVFAHPETLVKVKDAE c a t t t t g c c t t c c t g t t t t t g c t c a c c c a g a a a c g c t g g t g a a a g t a a a a g a t g c t g a a g g t a a a a c g g a a g g a c a a a a a c g a g t g g g t c t t t g c g a c c a c t t t c a t t t t c t a c g a c t t c

3360 40 45 50 55 DQLGARVGYIELDLNSGKIL AmpR DQLGARVGYIELDLNSGKIL a t c a g t t g g g t g c a c g a g t g g g t t a c a t c g a a c t g g a t c t c a a c a g c g g t a a g a t c c t t g t a g t c a a c c c a c g t g c t c a c c c a a t g t a g c t t g a c c t a g a g t t g t c g c c a t t c t a g g a a c

3420 60 65 70 75 ESFRPEERFPMMSTFKVLLC AmpR C G A T A C A C Amp-R ESFRPEERFPMMSTFKVLLC a g a g t t t t c g c c c c g a a g a a c g t t t t c c a a t g a t g a g c a c t t t t a a a g t t c t g c t a t g t g t c t c a a a a g c g g g g c t t c t t g c a a a a g g t t a c t a c t c g t g a a a a t t t c a a g a c g a t a c a c

3480 80 85 90 95 GAVLSRVDAGQEQLGRRIHY AmpR C G C G C C A T A A T A Amp-R GAVLSRVDAGQEQLGRRIHY g c g c g g t a t t a t c c c g t g t t g a c g c c g g g c a a g a g c a a c t c g g t c g c c g c a t a c a c t a t t c g c g c c a t a a t a g g g c a c a a c t g c g g c c c g t t c t c g t t g a g c c a g c g g c g t a t g t g a t a a

3540 100 105 110 115 SQNDLVEYSPVTEKHLTDGM AmpR SQNDLVEYSPVTEKHLTDGM c t c a g a a t g a c t t g g t t g a g t a c t c a c c a g t c a c a g a a a a g c a t c t t a c g g a t g g c a t g a g a g t c t t a c t g a a c c a a c t c a t g a g t g g t c a g t g t c t t t t c g t a g a a t g c c t a c c g t a c t

3600 120 125 130 135 TVRELCSAAITMSDNTAANL AmpR TVRELCSAAITMSDNTAANL c a g t a a g a g a a t t a t g c a g t g c t g c c a t a a c c a t g a g t g a t a a c a c t g c g g c c a a c t t a c g t c a t t c t c t t a a t a c g t c a c g a c g g t a t t g g t a c t c a c t a t t g t g a c g c c g g t t g a a t g

PvuI 3660 140 145 150 155 LLTTIGGPKELTAFLHNMGD AmpR LLTTIGGPKELTAFLHNMGD t t c t g a c a a c g a t c g g a g g a c c g a a g g a g c t a a c c g c t t t t t t g c a c a a c a t g g g g g a t c a a g a c t g t t g c t a g c c t c c t g g c t t c c t c g a t t g g c g a a a a a a c g t g t t g t a c c c c c t a g

3720 160 165 170 175 HVTRLDRWEPELNEAIPNDE AmpR HVTRLDRWEPELNEAIPNDE a t g t a a c t c g c c t t g a t c g t t g g g a a c c g g a g c t g a a t g a a g c c a t a c c a a a c g a c g a g c t a c a t t g a g c g g a a c t a g c a a c c c t t g g c c t c g a c t t a c t t c g g t a t g g t t t g c t g c t c g

FspI 3780 180 185 190 195 RDTTMPAAMATTLRKLLTGE AmpR RDTTMPAAMATTLRKLLTGE g t g a c a c c a c g a t g c c t g c a g c a a t g g c a a c a a c g t t g c g c a a a c t a t t a a c t g g c g a a c c a c t g t g g t g c t a c g g a c g t c g t t a c c g t t g t t g c a a c g c g t t t g a t a a t t g a c c g c t t g

3840 200 205 210 215 LLTLASRQQLIDWMEADKVA AmpR LLTLASRQQLIDWMEADKVA t a c t t a c t c t a g c t t c c c g g c a a c a a t t a a t a g a c t g g a t g g a g g c g g a t a a a g t t g c a g a t g a a t g a g a t c g a a g g g c c g t t g t t a a t t a t c t g a c c t a c c t c c g c c t a t t t c a a c g t c

3900 220 225 230 235 GPLLRSALPAGWFIADKSGA AmpR GPLLRSALPAGWFIADKSGA g a c c a c t t c t g c g c t c g g c c c t t c c g g c t g g c t g g t t t a t t g c t g a t a a a t c t g g a g c c g c t g g t g a a g a c g c g a g c c g g g a a g g c c g a c c g a c c a a a t a a c g a c t a t t t a g a c c t c g g c

3960 240 245 250 255 GERGSRGIIAALGPDGKPSR AmpR GERGSRGIIAALGPDGKPSR g t g a g c g t g g g t c t c g c g g t a t c a t t g c a g c a c t g g g g c c a g a t g g t a a g c c c t c c c g t a c a c t c g c a c c c a g a g c g c c a t a g t a a c g t c g t g a c c c c g g t c t a c c a t t c g g g a g g g c a t

4020 260 265 270 275 IVVIYTTGSQATMDERNRQI AmpR IVVIYTTGSQATMDERNRQI t c g t a g t t a t c t a c a c g a c g g g g a g t c a g g c a a c t a t g g a t g a a c g a a a t a g a c a g a t c g a g c a t c a a t a g a t g t g c t g c c c c t c a g t c c g t t g a t a c c t a c t t g c t t t a t c t g t c t a g c

4080 280 285 AEIGASLIKHW* AmpR AEIGASLIKHW* c t g a g a t a g g t g c c t c a c t g a t t a a g c a t t g g t a a c t g t c a g a c c a a g t t t a c t c a t a t a g a c t c t a t c c a c g g a g t g a c t a a t t c g t a a c c a t t g a c a g t c t g g t t c a a a t g a g t a t a t

4140 t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a

4200 t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g

4260 ori c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a

4320 ori t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t

4380 ori c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t t c t t c t a g g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a a g a a g a t c

4440 ori t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g

4500 ori t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c

4560 ori a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t

4620 ori c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a

4680 ori g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c

4740 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g

4800 ori c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g

4860 ori g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g

4920 c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c

4980 A G C G A G T C A G T G A L4440 c c t t t g a g t g a g c t g a t a c c g c t c g c c g c a g c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g g a a a c t c a c t c g a c t a t g g c g a g c g g c g t c g g c t t g c t g g c t c g c g t c g c t c a g t c a c t

BspQISapI 5040 G C G A G L4440 g c g a g g a a g c g g a a g a g c g c c c a a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c c g c t c c t t c g c c t t c t c g c g g g t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g

5100 a t t a a t g c a g c t g g c a c g a c a g g t t t c c c g a c t g g a a a g c g g g c a g t g a g c g c a a c g c a a t a a t t a c g t c g a c c g t g c t g t c c a a a g g g c t g a c c t t t c g c c c g t c a c t c g c g t t g c g t t

5160 -35 CAP binding site lac promoter t t a a t g t g a g t t a g c t c a c t c a t t a g g c a c c c c a g g c t t t a c a c t t t a t g c t t c c g g c t c a a t t a c a c t c a a t c g a g t g a g t a a t c c g t g g g g t c c g a a a t g t g a a a t a c g a a g g c c g a g

5220 -10 lac promoter lac operator M13 rev A G C G G A T A A C A A T T T C A C A C A G G M13/pUC Reverse C A G G A A A C A G C T A T G A C M13 Reverse g t a t g t t g t g t g g a a t t g t g a g c g g a t a a c a a t t t c a c a c a g g a a a c a g c t a t g a c c a t g c a t a c a a c a c a c c t t a a c a c t c g c c t a t t g t t a a a g t g t g t c c t t t g t c g a t a c t g g t a c

5280 a t t a c g c c a a g c t c t a g c t a g a g g t c g a c c a a t t c t c a t g t t t g a c a g c t t a t c a t c g c a t a a t g c g g t t c g a g a t c g a t c t c c a g c t g g t t a a g a g t a c a a a c t g t c g a a t a g t a g c g t

5340 g a t c c g g g c a a c g t t g t t g c c a t t g c t g c a g g c g c a g a a c t g g t a g g t a t g g a a g a t c t a c t a g g c c c g t t g c a a c a a c g g t a a c g a c g t c c g c g t c t t g a c c a t c c a t a c c t t c t a g a t

5400 t a c a t t g a a t c a a t a t t g g c a a t t a g c c a t a t t a g t c a t t g g t t a t a t a g c a t a a a t c a a a t g t a a c t t a g t t a t a a c c g t t a a t c g g t a t a a t c a g t a a c c a a t a t a t c g t a t t t a g t t

5460 t a t t g g c t a t t g g c c a t t g c a t a c g t t g t a t c t a t a t c a t a a t a t g t a c a t t t a t a t t g g a t a a c c g a t a a c c g g t a a c g t a t g c a a c a t a g a t a t a g t a t t a t a c a t g t a a a t a t a a c c

5520 CMV enhancer c t c a t g t c c a a t a t g a c c g c c a t g t t g a c a t t g a t t a t t g a c t a g t t a t t a a t a g t a a t c g a g t a c a g g t t a t a c t g g c g g t a c a a c t g t a a c t a a t a a c t g a t c a a t a a t t a t c a t t a g

5580 CMV enhancer a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a t a t g g a g t t c c g c g t t a c a t a a c t t a c g g t t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t a t a c c t c a a g g c g c a a t g t a t t g a a t g c c a

5640 CMV enhancer a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c c c c g c c c a t t g a c g t c a a t a a t g a c g t a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g g g g c g g g t a a c t g c a g t t a t t a c t g c a t

5700 CMV enhancer t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c a t t g a c g t c a a t g g g t g g a g t a t t t a c g a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g t a a c t g c a g t t a c c c a c c t c a t a a a t g c

5760 CMV enhancer g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t a t c a t a t g c c a a g t c c g c c c c c t a t t g a c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a t a g t a t a c g g t t c a g g c g g g g g a t a a c t

5820 CMV enhancer c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t a t g c c c a g t a c a t g a c c t t a c g g g a c t t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a t a c g g g t c a t g t a c t g g a a t g c c c t g a a

SnaBI 5880 CMV enhancer CMV promoter t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t c g c t a t t a c c a t g g t g a t g c g g t t t t g a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t a g c g a t a a t g g t a c c a c t a c g c c a a a a c

5940 CMV promoter g c a g t a c a c c a a t g g g c g t g g a t a g c g g t t t g a c t c a c g g g g a t t t c c a a g t c t c c a c c c c g t c a t g t g g t t a c c c g c a c c t a t c g c c a a a c t g a g t g c c c c t a a a g g t t c a g a g g t g g g

6000 CMV promoter c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c a a a a t c a a c g g g a c t t t c c a a a a t g t c g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g t t t t a g t t g c c c t g a a a g g t t t t a c a g c

6060 CMV promoter C G C A A A T G G G C G G T A G G C G T G CMV-F t a a t a a c c c c g c c c c g t t g a c g c a a a t g g g c g g t a g g c g t g t a c g g t g g g a g g t c t a t a t a t t a t t g g g g c g g g g c a a c t g c g t t t a c c c g c c a t c c g c a c a t g c c a c c c t c c a g a t a t a

Eco53kISacI 6120 CMV promoter A G C T C G T T T A G T G A A C C G T C A G A T C LNCX a a g c a g a g c t c g t t t a g t g a a c c g t c a g a t c c t c a c t c t c t t c c g c a t c g c t g t c t g c g a t t c g t c t c g a g c a a a t c a c t t g g c a g t c t a g g a g t g a g a g a a g g c g t a g c g a c a g a c g c t

6180 g g g c c a g c t g t t g g g c t c g c g g t t g a g g a c a a a c t c t t c g c g g t c t t t c c a g t a c t c t t g c c c g g t c g a c a a c c c g a g c g c c a a c t c c t g t t t g a g a a g c g c c a g a a a g g t c a t g a g a a c

6240 g a t c g g a a a c c c g t c g g c c t c c g a a c g g t a c t c c g c c a c c g a g g g a c c t g a g c g a g t c c g c t a g c c t t t g g g c a g c c g g a g g c t t g c c a t g a g g c g g t g g c t c c c t g g a c t c g c t c a g g c

PaeR7IXhoI 6300 c a t c g a c c g g a t c g g a a a a c c t c t c g a g a a a g g c g t c t a a c c a g t c a c a g t c g c a a g g t a g t a g c t g g c c t a g c c t t t t g g a g a g c t c t t t c c g c a g a t t g g t c a g t g t c a g c g t t c c a t

BlpI 6360 g g c t g a g c a c c g t g g c g g g c g g c a g c g g g t g g c g g t c g g g g t t g t t t c t g g c g g a g g t g c c c g a c t c g t g g c a c c g c c c g c c g t c g c c c a c c g c c a g c c c c a a c a a a g a c c g c c t c c a c g

BsmBIEsp3I 6420 t g c t g a t g a t g t a a t t a a a g t a g g c g g t c t t g a g a c g g c g g a t g g t c g a g g t g a g g t g t g a c g a c t a c t a c a t t a a t t t c a t c c g c c a g a a c t c t g c c g c c t a c c a g c t c c a c t c c a c a c

6480 g c a g g c t t g a g a t c c a g c t g t t g g g g t g a g t a c t c c c t c t c a a a a g c g g g c a t t a c t t c t c g t c c g a a c t c t a g g t c g a c a a c c c c a c t c a t g a g g g a g a g t t t t c g c c c g t a a t g a a g a

6540 *NGFVLLIQYEGPGIQ g c g c t a a g a t t g t c a g t t t c c a a a a a c g a g g a g g a t t t g a t a t t c a c c t g g c c c g a t c t g c g c g a t t c t a a c a g t c a a a g g t t t t t g c t c c t c c t a a a c t a t a a g t g g a c c g g g c t a g a c

6600 T T G C C T T T C T C T C C A C A G G T pMT2-F GYVQTVIVDVKGKEGCTDVG g c c a t a c a c t t g a g t g a c a a t g a c a t c c a c t t t g c c t t t c t c t c c a c a g g t g t c c a c t c c c g g t a t g t g a a c t c a c t g t t a c t g t a g g t g a a a c g g a a a g a g a g g t g t c c a c a g g t g a g g

EagINotIPmeI 6660 Kozak sequence MAPAAREARL LDLNLSRGGGHGRGGSFGSQ *VAAAVMAGAALSALS c a g g t c c a a g t t t a a a c t g c g g c c g c c a c c a t g g c c c c t g c c g c c a g a g a a g c c a g a c t g g t c c a g g t t c a a a t t t g a c g c c g g c g g t g g t a c c g g g g a c g g c g g t c t c t t c g g t c t g a c

6720 PPRSPGLRWALPLLLLLLRL GGSGRSQSPGQRQQQEQQSQ GGLDGPSLHARGRSSSRSLS c c c c c c a g a t c c c c t g g a c t g a g a t g g g c c c t g c c t c t g c t g c t g c t c c t g c t g a g a c t g g g g g g g t c t a g g g g a c c t g a c t c t a c c c g g g a c g g a g a c g a c g a c g a g g a c g a c t c t g a c

6780 GQILCAGGTPSPIPDPSVAT ALDQTGASGGARDGIRAHSC PWIRHAPPVGLGIGSGLTAV g g c c a g a t c c t g t g t g c c g g c g g a a c c c c c a g c c c t a t c c c c g a t c c t a g c g t g g c t a c a c c g g t c t a g g a c a c a c g g c c g c c t t g g g g g t c g g g a t a g g g g c t a g g a t c g c a c c g a t g t

6840 VATGENGITQISSTAESFHK HGGSFVADGLDAAGGLAEVL TAVPSFPMVWILLVASLKWL g t g g c c a c c g g a g a a a a c g g c a t c a c c c a g a t c a g c a g c a c c g c c g a g a g c t t c c a c a a g c a c c g g t g g c c t c t t t t g c c g t a g t g g g t c t a g t c g t c g t g g c g g c t c t c g a a g g t g t t c

6900 QNGTGTPQVETNTSEDGESS LVAGAGGLHLCVGALVALAA CFPVPVGWTSVFVLSSPSLL c a g a a c g g c a c c g g c a c c c c c c a g g t g g a g a c a a a c a c c a g c g a g g a c g g c g a g a g c a g c g t c t t g c c g t g g c c g t g g g g g g t c c a c c t c t g t t t g t g g t c g c t c c t g c c g c t c t c g t c g

6960 GANDSLRTPEQGSNGTDGAS AGVVAQSGGLLAAIAGIAGA PALSLRLVGSCPLLPVSPAL g g c g c c a a c g a c a g c c t g a g a a c c c c c g a g c a g g g c a g c a a t g g c a c c g a t g g c g c c a g c c c g c g g t t g c t g t c g g a c t c t t g g g g g c t c g t c c c g t c g t t a c c g t g g c t a c c g c g g t c g

7020 QKTPSSTGPSPVFDIKAVSI LFGRAAGAGARHEVDLGHAD WFVGLLVPGLGTNSMLATLM c a g a a a a c c c c t a g c a g c a c c g g c c c c a g c c c t g t g t t c g a c a t c a a g g c c g t g a g c a t c g t c t t t t g g g g a t c g t c g t g g c c g g g g t c g g g a c a c a a g c t g t a g t t c c g g c a c t c g t a g

7080 SPTNVILTWKSNDTAASEYK AGGVHDQGPLGVVGGGALVF LGVLTIRVQFDLSVAALSYL a g c c c c a c c a a c g t g a t c c t g a c c t g g a a g t c c a a c g a c a c c g c c g c c a g c g a g t a c a a a t c g g g g t g g t t g c a c t a g g a c t g g a c c t t c a g g t t g c t g t g g c g g c g g t c g c t c a t g t t t

7140 YVVKHKMENEKTITVVHQPW VHDLVLHFVLFGDGHDM YTTLCLISFSFVM t a c g t g g t c a a g c a c a a g a t g g a a a a c g a g a a a a c c a t c a c c g t g g t c c a t c a g c c c t g g a t g c a c c a g t t c g t g t t c t a c c t t t t g c t c t t t t g g t a g t g g c a c c a g g t a g t c g g g a c c

7200 CNITGLRPATSYVFSITPGI t g t a a c a t c a c t g g c c t g a g g c c c g c c a c c t c c t a c g t g t t c a g c a t c a c c c c c g g c a t c a c a t t g t a g t g a c c g g a c t c c g g g c g g t g g a g g a t g c a c a a g t c g t a g t g g g g g c c g t a g

7260 GNETWGDPRVIKVITEPIPV g g c a a c g a g a c a t g g g g c g a c c c t a g a g t g a t c a a a g t g a t c a c c g a g c c c a t c c c c g t g c c g t t g c t c t g t a c c c c g c t g g g a t c t c a c t a g t t t c a c t a g t g g c t c g g g t a g g g g c a c

7320 SDLRVALTGVRKAALSWSNG t c c g a t c t g a g a g t g g c c c t g a c c g g c g t g a g a a a g g c c g c c c t g a g c t g g t c t a a t g g c a g g c t a g a c t c t c a c c g g g a c t g g c c g c a c t c t t t c c g g c g g g a c t c g a c c a g a t t a c c g

7380 NGTASCRVLLESIGSHEELT a a c g g c a c c g c c a g c t g c a g a g t g c t g c t g g a a a g c a t c g g c a g c c a c g a g g a a c t g a c c t t g c c g t g g c g g t c g a c g t c t c a c g a c g a c c t t t c g t a g c c g t c g g t g c t c c t t g a c t g g

7440 QDSRLQVNISGLKPGVQYNI c a g g a c a g c c g g c t c c a g g t c a a c a t c a g c g g c c t g a a g c c t g g c g t g c a g t a c a a c a t c g t c c t g t c g g c c g a g g t c c a g t t g t a g t c g c c g g a c t t c g g a c c g c a c g t c a t g t t g t a g

7500 NPYLLQSNKTKGDPLGTEGG a a c c c c t a t c t g c t g c a g a g c a a c a a g a c c a a g g g a g a c c c t c t g g g a a c a g a g g g c g g c t t g g g g a t a g a c g a c g t c t c g t t g t t c t g g t t c c c t c t g g g a g a c c c t t g t c t c c c g c c g

7560 LDASNTERSRAGSPTAPVHD c t g g a c g c c a g c a a c a c c g a g a g a a g c a g a g c c g g c a g c c c t a c a g c c c c t g t g c a c g a c g a c c t g c g g t c g t t g t g g c t c t c t t c g t c t c g g c c g t c g g g a t g t c g g g g a c a c g t g c t g

7620 ESLVGPVDPSSGQQSRDTEV g a g t c t c t c g t g g g c c c c g t g g a t c c t a g c a g c g g c c a g c a g a g c a g a g a c a c c g a a g t g c t c a g a g a g c a c c c g g g g c a c c t a g g a t c g t c g c c g g t c g t c t c g t c t c t g t g g c t t c a c

XcmI 7680 LLVGLEPGTRYNATVYSQAA c t g c t c g t g g g c c t g g a a c c c g g c a c c a g a t a c a a c g c c a c c g t g t a c a g c c a g g c c g c c g a c g a g c a c c c g g a c c t t g g g c c g t g g t c t a t g t t g c g g t g g c a c a t g t c g g t c c g g c g g

7740 NGTEGQPQAIEFRTNAIQVF a a t g g c a c a g a a g g c c a g c c c c a g g c c a t c g a g t t c c g g a c c a a c g c c a t c c a g g t g t t c t t a c c g t g t c t t c c g g t c g g g g t c c g g t a g c t c a a g g c c t g g t t g c g g t a g g t c c a c a a g

7800 DVTAVNISATSLTLIWKVSD g a c g t g a c c g c c g t g a a t a t c a g c g c c a c c t c c c t g a c c c t g a t c t g g a a g g t g t c c g a c c t g c a c t g g c g g c a c t t a t a g t c g c g g t g g a g g g a c t g g g a c t a g a c c t t c c a c a g g c t g

7860 NESSSNYTYKIHVAGETDSS a a c g a g a g c a g c a g c a a c t a c a c c t a c a a g a t c c a c g t g g c c g g c g a g a c a g a c a g c a g c t t g c t c t c g t c g t c g t t g a t g t g g a t g t t c t a g g t g c a c c g g c c g c t c t g t c t g t c g t c g

7920 NLNVSEPRAVIPGLRSSTFY a a c c t g a a c g t g t c c g a g c c c a g a g c c g t g a t c c c t g g c c t g c g g a g c a g c a c c t t c t a c t t g g a c t t g c a c a g g c t c g g g t c t c g g c a c t a g g g a c c g g a c g c c t c g t c g t g g a a g a t g

7980 NITVCPVLGDIEGTPGFLQV a a c a t c a c c g t g t g c c c c g t g c t g g g c g a t a t c g a g g g c a c c c c c g g c t t t c t c c a g g t g t t g t a g t g g c a c a c g g g g c a c g a c c c g c t a t a g c t c c c g t g g g g g c c g a a a g a g g t c c a c

8040 HTPPVPVSDFRVTVVSTTEI c a c a c c c c c c c t g t g c c c g t g t c t g a c t t c a g a g t g a c c g t g g t g t c c a c c a c a g a g a t c g t g t g g g g g g g a c a c g g g c a c a g a c t g a a g t c t c a c t g g c a c c a c a g g t g g t g t c t c t a g

8100 GLAWSSHDAESFQMHITQEG g g c c t g g c c t g g t c t a g c c a c g a c g c c g a g t c c t t c c a g a t g c a c a t c a c c c a g g a a g g c c c g g a c c g g a c c a g a t c g g t g c t g c g g c t c a g g a a g g t c t a c g t g t a g t g g g t c c t t c c g

8160 AGNSRVEITTNQSIIIGGLF g c c g g a a a c a g c c g g g t g g a g a t c a c c a c c a a c c a g a g c a t c a t c a t c g g c g g c c t g t t c c g g c c t t t g t c g g c c c a c c t c t a g t g g t g g t t g g t c t c g t a g t a g t a g c c g c c g g a c a a g

8220 PGTKYCFEIVPKGPNGTEGA c c t g g a a c c a a a t a c t g c t t c g a g a t c g t g c c c a a g g g c c c t a a t g g a a c c g a g g g c g c t g g a c c t t g g t t t a t g a c g a a g c t c t a g c a c g g g t t c c c g g g a t t a c c t t g g c t c c c g c g a

8280 SRTVCNRTVPSAVFDIHVVY t c c a g a a c c g t g t g c a a c a g a a c c g t g c c c t c c g c c g t g t t c g a t a t c c a c g t g g t g t a c a g g t c t t g g c a c a c g t t g t c t t g g c a c g g g a g g c g g c a c a a g c t a t a g g t g c a c c a c a t g

8340 VTTTEMWLDWKSPDGASEYV g t g a c c a c a a c t g a g a t g t g g c t g g a c t g g a a g t c t c c t g a c g g c g c c t c c g a g t a c g t g c a c t g g t g t t g a c t c t a c a c c g a c c t g a c c t t c a g a g g a c t g c c g c g g a g g c t c a t g c a c

8400 YHLVIESKHGSNHTSTYDKA t a c c a c c t g g t c a t c g a g a g c a a g c a c g g c a g c a a c c a c a c c a g c a c c t a c g a c a a g g c c a t g g t g g a c c a g t a g c t c t c g t t c g t g c c g t c g t t g g t g t g g t c g t g g a t g c t g t t c c g g

8460 ITLQGLIPGTLYNITISPEV a t c a c c c t g c a g g g a c t g a t t c c a g g c a c c c t g t a c a a t a t c a c c a t c a g c c c c g a g g t g t a g t g g g a c g t c c c t g a c t a a g g t c c g t g g g a c a t g t t a t a g t g g t a g t c g g g g c t c c a c

8520 DHVWGDPNSTAQYTRPSNVS g a c c a c g t g t g g g g c g a t c c t a a c a g c a c c g c c c a g t a c a c c a g a c c c a g c a a c g t g t c c c t g g t g c a c a c c c c g c t a g g a t t g t c g t g g c g g g t c a t g t g g t c t g g g t c g t t g c a c a g g

8580 NIDVSTNTTAATLSWQNFDD a a c a t c g a c g t g t c c a c c a a c a c c a c a g c c g c c a c c c t g t c c t g g c a g a a c t t c g a c g a c t t g t a g c t g c a c a g g t g g t t g t g g t g t c g g c g g t g g g a c a g g a c c g t c t t g a a g c t g c t g

8640 ASPTYSYCLLIEKAGNSSNA g c c a g c c c c a c c t a c a g c t a c t g c c t g c t g a t c g a g a a g g c c g g c a a c t c c a g c a a t g c c c g g t c g g g g t g g a t g t c g a t g a c g g a c g a c t a g c t c t t c c g g c c g t t g a g g t c g t t a c g g

BstEII 8700 TQVVTDIGITDATVTELIPG a c c c a g g t g g t c a c c g a c a t c g g c a t c a c c g a t g c c a c c g t g a c a g a g c t g a t c c c c g g c t g g g t c c a c c a g t g g c t g t a g c c g t a g t g g c t a c g g t g g c a c t g t c t c g a c t a g g g g c c g

8760 SSYTVEIFAQVGDGIKSLEP a g c a g c t a c a c c g t g g a g a t c t t c g c c c a g g t c g g c g a c g g c a t c a a g t c c c t g g a a c c t t c g t c g a t g t g g c a c c t c t a g a a g c g g g t c c a g c c g c t g c c g t a g t t c a g g g a c c t t g g a

8820 GRKSFCTDPASMASFDCEVV g g c c g g a a g t c c t t c t g c a c c g a c c c t g c c a g c a t g g c c a g c t t c g a c t g c g a g g t g g t g c c g g c c t t c a g g a a g a c g t g g c t g g g a c g g t c g t a c c g g t c g a a g c t g a c g c t c c a c c a c

8880 PKEPALVLKWTCPPGANAGF c c c a a a g a a c c c g c c c t g g t c c t g a a a t g g a c c t g t c c c c c t g g c g c c a a t g c c g g c t t c g g g t t t c t t g g g c g g g a c c a g g a c t t t a c c t g g a c a g g g g g a c c g c g g t t a c g g c c g a a g

8940 ELEVSSGAWNNATHLESCSS g a g c t g g a a g t g t c t a g c g g c g c c t g g a a t a a c g c c a c c c a c c t g g a a a g c t g c a g c a g c c t c g a c c t t c a c a g a t c g c c g c g g a c c t t a t t g c g g t g g g t g g a c c t t t c g a c g t c g t c g

9000 ENGTEYRTEVTYLNFSTSYN g a g a a c g g g a c c g a g t a c c g g a c c g a a g t g a c c t a c c t g a a c t t c a g c a c c a g c t a c a a c c t c t t g c c c t g g c t c a t g g c c t g g c t t c a c t g g a t g g a c t t g a a g t c g t g g t c g a t g t t g

9060 ISITTVSCGKMAAPTRNTCT a t t a g c a t c a c c a c c g t g t c c t g c g g c a a g a t g g c c g c t c c c a c c c g g a a t a c c t g t a c c t a a t c g t a g t g g t g g c a c a g g a c g c c g t t c t a c c g g c g a g g g t g g g c c t t a t g g a c a t g g

9120 TGITDPPPPDGSPNITSVSH a c c g g c a t c a c a g a c c c c c c t c c c c c c g a t g g c a g c c c c a a c a t c a c c t c c g t g a g c c a c t g g c c g t a g t g t c t g g g g g g a g g g g g g c t a c c g t c g g g g t t g t a g t g g a g g c a c t c g g t g

StuI 9180 NSVKVKFSGFEASHGPIKAY a a c a g c g t g a a a g t g a a g t t c a g c g g c t t c g a g g c c a g c c a c g g c c c t a t c a a g g c c t a c t t g t c g c a c t t t c a c t t c a a g t c g c c g a a g c t c c g g t c g g t g c c g g g a t a g t t c c g g a t g

FseI 9240 AVILTTGEAGHPSADVLKYT g c c g t g a t t c t g a c a a c a g g c g a g g c c g g c c a c c c t t c t g c c g a c g t g c t g a a g t a c a c c c g g c a c t a a g a c t g t t g t c c g c t c c g g c c g g t g g g a a g a c g g c t g c a c g a c t t c a t g t g g

9300 YEDFKKGASDTYVTYLIRTE t a c g a g g a c t t c a a g a a g g g c g c c a g c g a c a c c t a c g t g a c a t a c c t g a t c c g g a c c g a g a t g c t c c t g a a g t t c t t c c c g c g g t c g c t g t g g a t g c a c t g t a t g g a c t a g g c c t g g c t c

9360 EKGRSQSLSEVLKYEIDVGN g a a a a g g g c a g a a g c c a g a g c c t g a g c g a g g t g c t g a a a t a c g a g a t c g a c g t g g g c a a c c t t t t c c c g t c t t c g g t c t c g g a c t c g c t c c a c g a c t t t a t g c t c t a g c t g c a c c c g t t g

9420 ESTTLGYYNGKLEPLGSYRA g a g t c c a c c a c c c t g g g c t a c t a c a a c g g c a a g c t g g a a c c c c t g g g c a g c t a c a g a g c c c t c a g g t g g t g g g a c c c g a t g a t g t t g c c g t t c g a c c t t g g g g a c c c g t c g a t g t c t c g g

9480 CVAGFTNITFHPQNKGLIDG t g c g t g g c c g g c t t c a c c a a t a t c a c a t t c c a c c c c c a a a a c a a g g g c c t g a t t g a c g g c a c g c a c c g g c c g a a g t g g t t a t a g t g t a a g g t g g g g g t t t t g t t c c c g g a c t a a c t g c c g

9540 AESYVSFSRYSDAVSLPQDP g c c g a g t c t t a c g t g t c c t t c a g c c g g t a c a g c g a c g c c g t g a g c c t g c c c c a g g a t c c a c g g c t c a g a a t g c a c a g g a a g t c g g c c a t g t c g c t g c g g c a c t c g g a c g g g g t c c t a g g t

BssHIIAscI 9548 GAP g g c g c g c c c c g c g c g g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.