Addgene pCAFNF-GFP - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pCAFNF-GFP


Map View

CsiI - SexAI * (6829) SspI (6719) XmnI (6514) Amp-R (6465 .. 6484) ScaI (6395) PvuI (6285) BsaI (5976) AhdI (5915) pBR322ori-F (5163 .. 5182) L4440 (4912 .. 4929) SV40 poly(A) signal AvrII (4689) StuI (4688) SfiI (4642) CAP binding site M13/pUC Reverse (4349 .. 4371) lac operator M13 Reverse (4336 .. 4352) HindIII (4318) PflMI (4060) rbglobpA-R (3976 .. 3995) Bglob-pA-R (3857 .. 3876) BglII (3854) BstXI (3833) Bsu36I (3784) NotI (3773) BsrGI (3760) EGFP-C (3704 .. 3725) SpeI (18) CMV enhancer SnaBI (359) BlpI (1154) PaqCI (1224) MreI - SgrAI (1311) pCAGGS-5 (1594 .. 1616) pCAG-F (1678 .. 1697) MluI (1739) BspEI (1777) BclI * (1790) Neo-R (1875 .. 1894) PvuII (2055) PflFI - Tth111I (2067) SphI (2354) RsrII (2465) Neo-F (2485 .. 2504) BstBI (2631) BspDI - ClaI (2790) FRT (minimal) PaeR7I - XhoI (2974) SwaI (2995) EcoRI (3001) Acc65I (3017) KpnI (3021) TspMI - XmaI (3028) SmaI (3030) AgeI (3038) EGFP-N (3096 .. 3117) EXFP-R (3357 .. 3376) pCAFNF-GFP 6833 bp

Sequence View

SpeI 60 CMV enhancer g t c g a c a t t g a t t a t t g a c t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a c a g c t g t a a c t a a t a a c t g a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t

120 CMV enhancer g c c c a t a t a t g g a g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c g g g t a t a t a c c t c a a g g c g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g

180 CMV enhancer c c a a c g a c c c c c g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g t t g c t g g g g g c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c

240 CMV enhancer g g a c t t t c c a t t g a c g t c a a t g g g t g g a c t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c c c t g a a a g g t a a c t g c a g t t a c c c a c c t g a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g

300 CMV enhancer MPSTPPIDVNDGKWP a t c a a g t g t a t c a t a t g c c a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g t a g t t c a c a t a g t a t a c g g t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c

SnaBI 360 CMV enhancer AWHYAQYMTLWDFPTWQYIY c c t g g c a t t a t g c c c a g t a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g g g a c c g t a a t a c g g g t c a t g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c

420 CMV enhancer chicken β- actin promoter VLVIAITMGRGEPHVLLHSP t a t t a g t c a t c g c t a t t a c c a t g g g t c g a g g t g a g c c c c a c g t t c t g c t t c a c t c t c c c c a t a a t c a g t a g c g a t a a t g g t a c c c a g c t c c a c t c g g g g t g c a a g a c g a a g t g a g a g g g g

480 chicken β- actin promoter HLPPLPTPNFVFIYFLIILC a t c t c c c c c c c c t c c c c a c c c c c a a t t t t g t a t t t a t t t a t t t t t t a a t t a t t t t g t g c a t a g a g g g g g g g g a g g g g t g g g g g t t a a a a c a t a a a t a a a t a a a a a a t t a a t a a a a c a c g t

540 chicken β- actin promoter SDGGGGGGGARQAGRGGARG g c g a t g g g g g c g g g g g g g g g g g g g g c g c g c g c c a g g c g g g g c g g g g c g g g g c g a g g g g c g c g c t a c c c c c g c c c c c c c c c c c c c c g c g c g c g g t c c g c c c c g c c c c g c c c c g c t c c c c g c

600 chicken β- actin promoter GAGRGGEVRRQPIRAARSES g g g c g g g g c g a g g c g g a g a g g t g c g g c g g c a g c c a a t c a g a g c g g c g c g c t c c g a a a g t t c c c g c c c c g c t c c g c c t c t c c a c g c c g c c g t c g g t t a g t c t c g c c g c g c g a g g c t t t c a a

660 chicken β- actin promoter FLLWRGGGGGGPIKSEARGG t c c t t t t a t g g c g a g g c g g c g g c g g c g g c g g c c c t a t a a a a a g c g a a g c g c g c g g c g g g c a g g a a a a t a c c g c t c c g c c g c c g c c g c c g c c g g g a t a t t t t t c g c t t c g c g c g c c g c c c g

720 chicken β- actin promoter chimeric intron RESLRCLRPVPRSAPPRAAR g g g a g t c g c t g c g t t g c c t t c g c c c c g t g c c c c g c t c c g c g c c g c c t c g c g c c g c c c g c c c c c t c a g c g a c g c a a c g g a a g c g g g g c a c g g g g c g a g g c g c g g c g g a g c g c g g c g g g c g g

780 chimeric intron PGSD* c c g g c t c t g a c t g a c c g c g t t a c t c c c a c a g g t g a g c g g g c g g g a c g g c c c t t c t c c t c c g g c c g a g a c t g a c t g g c g c a a t g a g g g t g t c c a c t c g c c c g c c c t g c c g g g a a g a g g a g g

840 chimeric intron *HRSTEKRHSRSL g g g c t g t a a t t a g c g c t t g g t t t a a t g a c g g c t c g t t t c t t t t c t g t g g c t g c g t g a a a g c c c g a c a t t a a t c g c g a a c c a a a t t a c t g c c g a g c a a a g a a a a g a c a c c g a c g c a c t t t c

900 chimeric intron RLPSRSPGKHPPSRSPPHTR *LAGPPGKTRPPAARPTRA c c t t a a a g g g c t c c g g g a g g g c c c t t t g t g c g g g g g g g a g c g g c t c g g g g g g t g c g t g c g g g a a t t t c c c g a g g c c c t c c c g g g a a a c a c g c c c c c c c t c g c c g a g c c c c c c a c g c a c g c

960 chimeric intron THTRPSRRTRGASGPPQSRQ HTHAHPAGRAARAARRSHAS t g t g t g t g t g c g t g g g g a g c g c c g c g t g c g g c c c g c g c t g c c c g g c g g c t g t g a g c g c t g a c a c a c a c a c g c a c c c c t c g c g g c g c a c g c c g g g c g c g a c g g g c c g c c g a c a c t c g c g a c

1020 chimeric intron PRPAPSQASRTHALPARGPA RARRPAKHAGRTRSPLAAPP c g g g c g c g g c g c g g g g c t t t g t g c g c t c c g c g t g t g c g c g a g g g g a g c g c g g c c g g g g g c g c c c g c g c c g c g c c c c g a a a c a c g c g a g g c g c a c a c g c g c t c c c c t c g c g c c g g c c c c c g

1080 chimeric intron TGRPAPPSRPSCLSRAPHTR PAGRHPPAALPVFAAHPTHA g g t g c c c c g c g g t g c g g g g g g g c t g c g a g g g g a a c a a a g g c t g c g t g c g g g g t g t g t g c g c c a c g g g g c g c c a c g c c c c c c c g a c g c t c c c c t t g t t t c c g a c g c a c g c c c c a c a c a c g c

1140 chimeric intron PPTLLPHPRPPRATVGGQVG HPPSCPTHARRDPQLGGRCG t g g g g g g g t g a g c a g g g g g t g t g g g c g c g g c g g t c g g g c t g t a a c c c c c c c c t g c a c c c c a c c c c c c c a c t c g t c c c c c a c a c c c g c g c c g c c a g c c c g a c a t t g g g g g g g g a c g t g g g g

BlpI 1200 chimeric intron RGSNSLVARSRTRPETRPTA GGRTASCPGAEPAPSRAPRP c c t c c c c g a g t t g c t g a g c a c g g c c c g g c t t c g g g t g c g g g g c t c c g t g c g g g g c g t g g c g g a g g g g c t c a a c g a c t c g t g c c g g g c c g a a g c c c a c g c c c c g a g g c a c g c c c c g c a c c g

PaqCI 1260 chimeric intron RPEGHRAPPPPLHPHRAPRP APSATGPPPHRCTPTGPPAP g c g g g g c t c g c c g t g c c g g g c g g g g g g t g g c g g c a g g t g g g g g t g c c g g g c g g g g c g g g g c g c c c c g a g c g g c a c g g c c c g c c c c c c a c c g c c g t c c a c c c c c a c g g c c c g c c c c g c c c c

SgrAIMreI 1320 chimeric intron RRPGPLARPLPAAAGSRRRS GGRAPSPEPSPRPPGPAGAA c c g c c t c g g g c c g g g g a g g g c t c g g g g g a g g g g c g c g g c g g c c c c g g a g c g c c g g c g g c t g g c g g a g c c c g g c c c c t c c c g a g c c c c c t c c c c g c g c c g c c g g g g c c t c g c g g c c g c c g a

1380 chimeric intron DLRPSGCGNGKITITRSPAP TSARRAAAM g t c g a g g c g c g g c g a g c c g c a g c c a t t g c c t t t t a t g g t a a t c g t g c g a g a g g g c g c a g g c a g c t c c g c g c c g c t c g g c g t c g g t a a c g g a a a a t a c c a t t a g c a c g c t c t c c c g c g t c c

1440 chimeric intron VEKTGFRASGFDPLRRRVGE g a c t t c c t t t g t c c c a a a t c t g g c g g a g c c g a a a t c t g g g a g g c g c c g c c g c a c c c c c t c c t g a a g g a a a c a g g g t t t a g a c c g c c t c g g c t t t a g a c c c t c c g c g g c g g c g t g g g g g a g

1500 chimeric intron LPRPRLPAAGAPLFHAPLAK t a g c g g g c g c g g g c g a a g c g g t g c g g c g c c g g c a g g a a g g a a a t g g g c g g g g a g g g c c t t a t c g c c c g c g c c c g c t t c g c c a c g c c g c g g c c g t c c t t c c t t t a c c c g c c c c t c c c g g a a

1560 chimeric intron TRRRAATGKEM c g t g c g t c g c c g c g c c g c c g t c c c c t t c t c c a t c t c c a g c c t c g g g g c t g c c g c a g g g g g g c a c g c a g c g g c g c g g c g g c a g g g g a a g a g g t a g a g g t c g g a g c c c c g a c g g c g t c c c c c

1620 chimeric intron G G T T C G G C T T C T G G C G T G T G A C C pCAGGS-5 a c g g c t g c c t t c g g g g g g g a c g g g g c a g g g c g g g g t t c g g c t t c t g g c g t g t g a c c g g c g t g c c g a c g g a a g c c c c c c c t g c c c c g t c c c g c c c c a a g c c g a a g a c c g c a c a c t g g c c g c

1680 chimeric intron G C A pCAG-F g c t c t a g a g c c t c t g c t a a c c a t g t t c a t g c c t t c t t c t t t t t c c t a c a g c t c c t g g g c a c g a g a t c t c g g a g a c g a t t g g t a c a a g t a c g g a a g a a g a a a a a g g a t g t c g a g g a c c c g t

MluI 1740 A C G T G C T G G T T A T T G T G pCAG-F a c g t g c t g g t t a t t g t g c t g t c t c a t c a t t t t g g c a a a g a a t t g a t t a a t t c g a g c g a a c t g c a c g a c c a a t a a c a c g a c a g a g t a g t a a a a c c g t t t c t t a a c t a a t t a a g c t c g c t t g

BclI*BspEI 1800 FRT (minimal) g c g t g a a g t t c c t a t t c t c t a g a a a g t a t a g g a a c t t c c g g a t t t g a t c t g a t c a a g a g a c g c a c t t c a a g g a t a a g a g a t c t t t c a t a t c c t t g a a g g c c t a a a c t a g a c t a g t t c t c t

1860 1 5 10 MIEQDGLHAGSPA NeoR/KanR MIEQDGLHAGSPA c a g g a t g a g g a t c g t t t c g c a t g a t t g a a c a a g a t g g a t t g c a c g c a g g t t c t c c g g c c g g t c c t a c t c c t a g c a a a g c g t a c t a a c t t g t t c t a c c t a a c g t g c g t c c a a g a g g c c g g c

1920 15 20 25 30 AWVERLFGYDWAQQTIGCSD NeoR/KanR G A T A A G C C G A T A C T G A C C C G Neo-R AWVERLFGYDWAQQTIGCSD c t t g g g t g g a g a g g c t a t t c g g c t a t g a c t g g g c a c a a c a g a c a a t c g g c t g c t c t g a t g g a a c c c a c c t c t c c g a t a a g c c g a t a c t g a c c c g t g t t g t c t g t t a g c c g a c g a g a c t a c

1980 35 40 45 50 AAVFRLSAQGRPVLFVKTDL NeoR/KanR AAVFRLSAQGRPVLFVKTDL c c g c c g t g t t c c g g c t g t c a g c g c a g g g g c g c c c g g t t c t t t t t g t c a a g a c c g a c c t g t g g c g g c a c a a g g c c g a c a g t c g c g t c c c c g c g g g c c a a g a a a a a c a g t t c t g g c t g g a c a

2040 55 60 65 70 SGALNELQDEAARLSWLATT NeoR/KanR SGALNELQDEAARLSWLATT c c g g t g c c c t g a a t g a a c t g c a g g a c g a g g c a g c g c g g c t a t c g t g g c t g g c c a c g a c g g g g c c a c g g g a c t t a c t t g a c g t c c t g c t c c g t c g c g c c g a t a g c a c c g a c c g g t g c t g c c

Tth111IPflFIPvuII 2100 75 80 85 90 GVPCAAVLDVVTEAGRDWLL NeoR/KanR GVPCAAVLDVVTEAGRDWLL g c g t t c c t t g c g c a g c t g t g c t c g a c g t t g t c a c t g a a g c g g g a a g g g a c t g g c t g c t a t c g c a a g g a a c g c g t c g a c a c g a g c t g c a a c a g t g a c t t c g c c c t t c c c t g a c c g a c g a t a

2160 95 100 105 110 LGEVPGQDLLSSHLAPAEKV NeoR/KanR LGEVPGQDLLSSHLAPAEKV *RVKSRGLFY t g g g c g a a g t g c c g g g g c a g g a t c t c c t g t c a t c t c a c c t t g c t c c t g c c g a g a a a g t a t a c c c g c t t c a c g g c c c c g t c c t a g a g g a c a g t a g a g t g g a a c g a g g a c g g c t c t t t c a t a

2220 115 120 125 130 SIMADAMRRLHTLDPATCPF NeoR/KanR SIMADAMRRLHTLDPATCPF GDHSICHPPQMRKIRSGAWE c c a t c a t g g c t g a t g c a a t g c g g c g g c t g c a t a c g c t t g a t c c g g c t a c c t g c c c a t t c g g g t a g t a c c g a c t a c g t t a c g c c g c c g a c g t a t g c g a a c t a g g c c g a t g g a c g g g t a a g c

2280 135 140 145 150 DHQAKHRIERARTRMEAGLV NeoR/KanR DHQAKHRIERARTRMEAGLV VVLRFMADLSCTSPHFGTKD a c c a c c a a g c g a a a c a t c g c a t c g a g c g a g c a c g t a c t c g g a t g g a a g c c g g t c t t g t c g t g g t g g t t c g c t t t g t a g c g t a g c t c g c t c g t g c a t g a g c c t a c c t t c g g c c a g a a c a g c

2340 155 160 165 170 DQDDLDEEHQGLAPAELFAR NeoR/KanR DQDDLDEEHQGLAPAELFAR ILIIQVFLMLPERWGFQEGP a t c a g g a t g a t c t g g a c g a a g a g c a t c a g g g g c t c g c g c c a g c c g a a c t g t t c g c c a g g c t a g t c c t a c t a g a c c t g c t t c t c g t a g t c c c c g a g c g c g g t c g g c t t g a c a a g c g g t c c g

SphI 2400 175 180 185 190 LKARMPDGEDLVVTHGDACL NeoR/KanR LKARMPDGEDLVVTHGDACL ELRAHGVALIEDHGMAIGAQ t c a a g g c g c g c a t g c c c g a c g g c g a g g a t c t c g t c g t g a c c c a t g g c g a t g c c t g c t t g c a g t t c c g c g c g t a c g g g c t g c c g c t c c t a g a g c a g c a c t g g g t a c c g c t a c g g a c g a a c g

2460 195 200 205 210 PNIMVENGRFSGFIDCGRLG NeoR/KanR PNIMVENGRFSGFIDCGRLG RIDHHFIAAKRSEDVTAPQT c g a a t a t c a t g g t g g a a a a t g g c c g c t t t t c t g g a t t c a t c g a c t g t g g c c g g c t g g g t g g c t t a t a g t a c c a c c t t t t a c c g g c g a a a a g a c c t a a g t a g c t g a c a c c g g c c g a c c c a c

RsrII 2520 215 220 225 230 VADRYQDIALATRDIAEELG NeoR/KanR C G T T G G C T A C C C G T G A T A T T Neo-F VADRYQDIALATRDIAEELG HRVAILVYRQSGTINSFLKA t g g c g g a c c g c t a t c a g g a c a t a g c g t t g g c t a c c c g t g a t a t t g c t g a a g a g c t t g g c g a c c g c c t g g c g a t a g t c c t g t a t c g c a a c c g a t g g g c a c t a t a a c g a c t t c t c g a a c c g c

2580 235 240 245 250 GEWADRFLVLYGIAAPDSQR NeoR/KanR GEWADRFLVLYGIAAPDSQR AFPSVAEEHKVTDGSGIRLA g c g a a t g g g c t g a c c g c t t c c t c g t g c t t t a c g g t a t c g c c g c t c c c g a t t c g c a g c g c a c g c t t a c c c g a c t g g c g a a g g a g c a c g a a a t g c c a t a g c g g c g a g g g c t a a g c g t c g c g t

BstBI 2640 255 260 265 IAFYRLLDEFF* NeoR/KanR IAFYRLLDEFF* DGEIAKKVLEESRSEPTRFS t c g c c t t c t a t c g c c t t c t t g a c g a g t t c t t c t g a g c g g g a c t c t g g g g t t c g a a a t g a c a g c g g a a g a t a g c g g a a g a a c t g c t c a a g a a g a c t c g c c c t g a g a c c c c a a g c t t t a c t g

2700 RGLSAWGAM c g a c c a a g c g a c g c c c a a c c t g c c a t c a c g a g a t t t c g a t t c c a c c g c c g c c t t c t a t g a g c t g g t t c g c t g c g g g t t g g a c g g t a g t g c t c t a a a g c t a a g g t g g c g g c g g a a g a t a c t

2760 a a g g t t g g g c t t c g g a a t c g t t t t c c g g g a c g c c g g c t g g a t g a t c c t c c a g c g c g g g g a t t c c a a c c c g a a g c c t t a g c a a a a g g c c c t g c g g c c g a c c t a c t a g g a g g t c g c g c c c c t

BspDIClaI 2820 SV40 poly(A) signal t c t c a t g c t g g a g t t c t t c g c c c a c c c c a t c g a t a a c t t g t t t a t t g c a g c t t a t a a t g g a g a g t a c g a c c t c a a g a a g c g g g t g g g g t a g c t a t t g a a c a a a t a a c g t c g a a t a t t a c c

2880 SV40 poly(A) signal t t a c a a a t a a a g c a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c a a t g t t t a t t t c g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g

2940 SV40 poly(A) signal FRT (minimal) t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t a t c a t g t c t g g a t c a a a t c c g a g a a a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a t a g t a c a g a c c t a g t t t a g g c t c t t

SwaIPaeR7IXhoI 3000 FRT (minimal) *I g t t c c t a t t c t c t a g a a a g t a t a g g a a c t t c a t c t c g a g t c g c t c g g t a c g a t t t a a a t t c a a g g a t a a g a g a t c t t t c a t a t c c t t g a a g t a g a g c t c a g c g a g c c a t g c t a a a t t t a a

AgeIAcc65IKpnITspMIXmaISmaIEcoRI 3060 1 M 1a V S EGFP 1 M 1a V S EGFP Kozak sequence MVS SNQLRRYRPGPIWRDGGHHA g a a t t c t g c a g t c g a c g g t a c c g c g g g c c c g g g a t c c a c c g g t c g c c a c c a t g g t g a g c a c t t a a g a c g t c a g c t g c c a t g g c g c c c g g g c c c t a g g t g g c c a g c g g t g g t a c c a c t c g t

3120 5 10 15 20 KGEELFTGVVPILVELDGDV EGFP 5 10 15 20 KGEELFTGVVPILVELDGDV EGFP G A C C A G C T C G A C C T G C C G C T G C EGFP-N KGEELFTGVVPILVELDGDV LALLQEGPHHGDQDLQVAVY a g g g c g a g g a g c t g t t c a c c g g g g t g g t g c c c a t c c t g g t c g a g c t g g a c g g c g a c g t a a t c c c g c t c c t c g a c a a g t g g c c c c a c c a c g g g t a g g a c c a g c t c g a c c t g c c g c t g c a t t

3180 25 30 35 40 NGHKFSVSGEGEGDATYGKL EGFP 25 30 35 40 NGHKFSVSGEGEGDATYGKL EGFP NGHKFSVSGEGEGDATYGKL VAVLEAHGALALAIGGVALQ a c g g c c a c a a g t t c a g c g t g t c c g g c g a g g g c g a g g g c g a t g c c a c c t a c g g c a a g c t g a t g c c g g t g t t c a a g t c g c a c a g g c c g c t c c c g c t c c c g c t a c g g t g g a t g c c g t t c g a c t

3240 45 50 55 60 TLKFICTTGKLPVPWPTLVT EGFP 45 50 55 60 TLKFICTTGKLPVPWPTLVT EGFP TLKFICTTGKLPVPWPTLVT GQLEDAGGALQGHGPGGEHG c c c t g a a g t t c a t c t g c a c c a c c g g c a a g c t g c c c g t g c c c t g g c c c a c c c t c g t g a c c a g g g a c t t c a a g t a g a c g t g g t g g c c g t t c g a c g g g c a c g g g a c c g g g t g g g a g c a c t g g t

3300 65 70 75 80 TLTYGVQCFSRYPDHMKQHD EGFP 65 70 75 80 TLTYGVQCFSRYPDHMKQHD EGFP TLTYGVQCFSRYPDHMKQHD GQGVAHLAEAAVGVVHLLVV c c c t g a c c t a c g g c g t g c a g t g c t t c a g c c g c t a c c c c g a c c a c a t g a a g c a g c a c g a c t g g g a c t g g a t g c c g c a c g t c a c g a a g t c g g c g a t g g g g c t g g t g t a c t t c g t c g t g c t g a

3360 85 90 95 100 FFKSAMPEGYVQERTIFFKD EGFP 85 90 95 100 FFKSAMPEGYVQERTIFFKD EGFP C T G C EXFP-R FFKSAMPEGYVQERTIFFKD EELGGHGFAVDLLAGDEELV t c t t c a a g t c c g c c a t g c c c g a a g g c t a c g t c c a g g a g c g c a c c a t c t t c t t c a a g g a c g a g a a g t t c a g g c g g t a c g g g c t t c c g a t g c a g g t c c t c g c g t g g t a g a a g a a g t t c c t g c

3420 105 110 115 120 DGNYKTRAEVKFEGDTLVNR EGFP 105 110 115 120 DGNYKTRAEVKFEGDTLVNR EGFP T G C C G T T G A T G T T C T G EXFP-R DGNYKTRAEVKFEGDTLVNR VAVVLGAGLHLELAVGQHVA a c g g c a a c t a c a a g a c c c g c g c c g a g g t g a a g t t c g a g g g c g a c a c c c t g g t g a a c c g c a t g c c g t t g a t g t t c t g g g c g c g g c t c c a c t t c a a g c t c c c g c t g t g g g a c c a c t t g g c g t

3480 125 130 135 140 IELKGIDFKEDGNILGHKLE EGFP 125 130 135 140 IELKGIDFKEDGNILGHKLE EGFP IELKGIDFKEDGNILGHKLE DLQLADVELLVAVDQPVLQL t c g a g c t g a a g g g c a t c g a c t t c a a g g a g g a c g g c a a c a t c c t g g g g c a c a a g c t g g a g t a g c t c g a c t t c c c g t a g c t g a a g t t c c t c c t g c c g t t g t a g g a c c c c g t g t t c g a c c t c a

3540 145 150 155 160 YNYNSHNVYIMADKQKNGIK EGFP 145 150 155 160 YNYNSHNVYIMADKQKNGIK EGFP YNYNSHNVYIMADKQKNGIK VVVVAVVDIDHGVLLLVADL a c a a c t a c a a c a g c c a c a a c g t c t a t a t c a t g g c c g a c a a g c a g a a g a a c g g c a t c a a g g t g t t g a t g t t g t c g g t g t t g c a g a t a t a g t a c c g g c t g t t c g t c t t c t t g c c g t a g t t c c

3600 165 170 175 180 VNFKIRHNIEDGSVQLADHY EGFP 165 170 175 180 VNFKIRHNIEDGSVQLADHY EGFP VNFKIRHNIEDGSVQLADHY HVELDAVVDLVAAHLEGVVV t g a a c t t c a a g a t c c g c c a c a a c a t c g a g g a c g g c a g c g t g c a g c t c g c c g a c c a c t a c c a c t t g a a g t t c t a g g c g g t g t t g t a g c t c c t g c c g t c g c a c g t c g a g c g g c t g g t g a t g g

3660 185 190 195 200 QQNTPIGDGPVLLPDNHYLS EGFP 185 190 195 200 QQNTPIGDGPVLLPDNHYLS EGFP QQNTPIGDGPVLLPDNHYLS LLVGGDAVAGHQQGVVVVQA a g c a g a a c a c c c c c a t c g g c g a c g g c c c c g t g c t g c t g c c c g a c a a c c a c t a c c t g a g c a t c g t c t t g t g g g g g t a g c c g c t g c c g g g g c a c g a c g a c g g g c t g t t g g t g a t g g a c t c g t

3720 205 210 215 220 TQSALSKDPNEKRDHMVLLE EGFP 205 210 215 220 TQSALSKDPNEKRDHMVLLE EGFP C A T G G T C C T G C T G G A G T EGFP-C TQSALSKDPNEKRDHMVLLE GLGGQAFVGVLLAIVHDQQL c c c a g t c c g c c c t g a g c a a a g a c c c c a a c g a g a a g c g c g a t c a c a t g g t c c t g c t g g a g t g g g t c a g g c g g g a c t c g t t t c t g g g g t t g c t c t t c g c g c t a g t g t a c c a g g a c g a c c t c a

NotIBsrGI 3780 225 230 235 FVTAAGITLGMDELYK* EGFP 225 230 235 FVTAAGITLGMDELYK * EGFP T C G T G EGFP-C FVTAAGITLGMDELYK* EHGGGPDSEAHVLQVLLAAA t c g t g a c c g c c g c c g g g a t c a c t c t c g g c a t g g a c g a g c t g t a c a a g t a a a g c g g c c g c a a g c a c t g g c g g c g g c c c t a g t g a g a g c c g t a c c t g c t c g a c a t g t t c a t t t c g c c g g c g t

BstXIBsu36I 3840 SRLHLSGILLHHSTHGIGQS c t c c t c a g g t g c a g g c t g c c t a t c a g a a g g t g g t g g c t g g t g t g g c c a a t g c c c t g g c t c g a g g a g t c c a c g t c c g a c g g a t a g t c t t c c a c c a c c g a c c a c a c c g g t t a c g g g a c c g a g

BglII 3900 A G A A A A A G G G A G A C G G T T T T Bglob-pA-R VFVVSIKKGRGFIIPVDHLG a c a a a t a c c a c t g a g a t c t t t t t c c c t c t g c c a a a a a t t a t g g g g a c a t c a t g a a g c c c c t g t t t a t g g t g a c t c t a g a a a a a g g g a g a c g g t t t t t a a t a c c c c t g t a g t a c t t c g g g g

3960 β- globin poly(A) signal KLM t t g a g c a t c t g a c t t c t g g c t a a t a a a g g a a a t t t a t t t t c a t t g c a a t a g t g t g t t g g a a a c t c g t a g a c t g a a g a c c g a t t a t t t c c t t t a a a t a a a a g t a a c g t t a t c a c a c a a c c t

4020 β- globin poly(A) signal G T G A G C C T T C C T G T A T A C C C rbglobpA-R a t t t t t t g t g t c t c t c a c t c g g a a g g a c a t a t g g g a g g g c a a a t c a t t t a a a a c a t c a g a t a a a a a a c a c a g a g a g t g a g c c t t c c t g t a t a c c c t c c c g t t t a g t a a a t t t t g t a g t c t

PflMI 4080 a t g a g t a t t t g g t t t a g a g t t t g g c a a c a t a t g c c a t a t g c t g g c t g c c a t g a a c a a a g g t a c t c a t a a a c c a a a t c t c a a a c c g t t g t a t a c g g t a t a c g a c c g a c g g t a c t t g t t t c c

4140 t g g c t a t a a a g a g g t c a t c a g t a t a t g a a a c a g c c c c c t g c t g t c c a t t c c t t a t t c c a t a c c g a t a t t t c t c c a g t a g t c a t a t a c t t t g t c g g g g g a c g a c a g g t a a g g a a t a a g g t a

4200 a g a a a a g c c t t g a c t t g a g g t t a g a t t t t t t t t a t a t t t t g t t t t g t g t t a t t t t t t t c t t c t t t t c g g a a c t g a a c t c c a a t c t a a a a a a a a t a t a a a a c a a a a c a c a a t a a a a a a a g a

4260 t t a a c a t c c c t a a a a t t t t c c t t a c a t g t t t t a c t a g c c a g a t t t t t c c t c c t c t c c t g a a a t t g t a g g g a t t t t a a a a g g a a t g t a c a a a a t g a t c g g t c t a a a a a g g a g g a g a g g a c t

HindIII 4320 c t a c t c c c a g t c a t a g c t g t c c c t c t t c t c t t a t g a a g a t c c c t c g a c c t g c a g c c c a a g g a t g a g g g t c a g t a t c g a c a g g g a g a a g a g a a t a c t t c t a g g g a g c t g g a c g t c g g g t t c

4380 M13 rev lac operator C A G T A T C G A C A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse c t t g g c g t a a t c a t g g t c a t a g c t g t t t c c t g t g t g a a a t t g t t a t c c g c t c a c a a t t c c g a a c c g c a t t a g t a c c a g t a t c g a c a a a g g a c a c a c t t t a a c a a t a g g c g a g t g t t a a g g

4440 -10 -35 lac promoter CAP binding site a c a c a a c a t a c g a g c c g g a a g c a t a a a g t g t a a a g c c t g g g g t g c c t a a t g a g t g a g c t a t g t g t t g t a t g c t c g g c c t t c g t a t t t c a c a t t t c g g a c c c c a c g g a t t a c t c a c t c g a t

4500 CAP binding site a c t c a c a t t a a t t g c g t t g c g c t c a c t g c c c g c t t t c c a g t c g g g a a a c c t g t c g t g c c a t g a g t g t a a t t a a c g c a a c g c g a g t g a c g g g c g a a a g g t c a g c c c t t t g g a c a g c a c g g t

4560 SV40 promoter SV40 ori g c g g a t c c g c a t c t c a a t t a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c c a t c c c g c g c c t a g g c g t a g a g t t a a t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g g t a g g g c

4620 SV40 promoter SV40 ori c c c c t a a c t c c g c c c a g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t t a t t g g g g a t t g a g g c g g g t c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a a t a a

SfiI 4680 SV40 promoter SV40 ori t a t g c a g a g g c c g a g g c c g c c t c g g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t a t a c g t c t c c g g c t c c g g c g g a g c c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a

StuIAvrII 4740 SV40 promoter SV40 poly(A) signal SV40 ori t t t g g a g g c c t a g g c t t t t g c a a a a a g c t a a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a a c c t c c g g a t c c g a a a a c g t t t t t c g a t t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t

4800 SV40 poly(A) signal a a t a a a g c a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t t t a t t t c g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a

4860 SV40 poly(A) signal g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t a t c a t g t c t g g a t c c g c t g c a t t a a t g a a t c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a t a g t a c a g a c c t a g g c g a c g t a a t t a c t t a

4920 G A G C G A G T G L4440 c g g c c a a c g c g c g g g g a g a g g c g g t t t g c g t a t t g g g c g c t c t t c c g c t t c c t c g c t c a c g c c g g t t g c g c g c c c c t c t c c g c c a a a c g c a t a a c c c g c g a g a a g g c g a a g g a g c g a g t g

4980 A C T G A G C G A L4440 t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a a g g c g g t a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t t c c g c c a

5040 a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a a a g g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t t t c c g g t

5100 ori g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t c c g c c c c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a g g c g g g

5160 ori c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g g a c t g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t g a

5220 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c c t t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g g a

5280 ori g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a a t g c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t t a c

5340 ori c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g c a g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c g t

5400 ori c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c a a g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g g t t

5460 ori c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g a g c g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c g t c t c g

5520 ori g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a c a c t a g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t g t g a t c

5580 ori a a g g a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g a g t t g g t t c c t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c t c a a c c

5640 ori t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g c a a g c a a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c g t t c g t

5700 ori g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g g g g t c c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g c c c c a g

5760 t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t c a a a a a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a g t t t t t c

5820 g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t c t a a a g t a t a t a c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t t t c a t a t a t

5880 285 280 275 *WHKILSAGIEAI AmpR *WHKILSAGIEAI t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t t a a t c a g t g a g g c a c c t a t c t c a g c g a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a a t t a g t c a c t c c g t g g a t a g a g t c g c t a

AhdI 5940 270 265 260 255 QRNREDMTAQSGTTYIVVIR AmpR QRNREDMTAQSGTTYIVVIR c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c t c c c c g t c g t g t a g a t a a c t a c g a t a c g g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g g g g c a g c a c a t c t a t t g a t g c t a t g c

BsaI 6000 250 245 240 235 SPKGDPGLAAIIGRSGREGA AmpR SPKGDPGLAAIIGRSGREGA g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c g a g a c c c a c g c t c a c c g g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t a c t a t g g c g c t c t g g g t g c g a g t g g c c g

6060 230 225 220 215 GSKDAIFWGAPLASRLLPGA AmpR GSKDAIFWGAPLASRLLPGA t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g a g c g c a g a a g t g g t c c t g c a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t c c c g g c t c g c g t c t t c a c c a g g a c g

6120 210 205 200 195 VKDAEMWDILQQRSALTLLE AmpR VKDAEMWDILQQRSALTLLE a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g g a a g c t a g a g t a a g t a g t t c t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c a a c g g c c c t t c g a t c t c a t t c a t c a a g

6180 190 185 180 175 GTLLKRLTTAMAVPMTTDRE AmpR GTLLKRLTTAMAVPMTTDRE g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a t t g c t a c a g g c a t c g t g g t g t c a c g c t c c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t a a c g a t g t c c g t a g c a c c a c a g t g c g a g

6240 170 165 160 155 DNPIAENLEPEWRDLRTVHD AmpR DNPIAENLEPEWRDLRTVHD g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c a a c g a t c a a g g c g a g t t a c a t g a t c c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g g t t g c t a g t t c c g c t c a a t g t a c t a g

PvuI 6300 150 145 140 135 GMNHLFATLEKPGGITTLLL AmpR GMNHLFATLEKPGGITTLLL c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t t c g g t c c t c c g a t c g t t g t c a g a a g t a a g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g c t a g c a a c a g t c t t c a t t

6360 130 125 120 115 NAATNDSMTIAASCLERVTM AmpR NAATNDSMTIAASCLERVTM g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a c t g c a t a a t t c t c t t a c t g t c a t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c g t c g t g a c g t a t t a a g a g a a t g a c a g t a

ScaI 6420 110 105 100 95 GDTLHKETVPSYEVLDNQSY AmpR GDTLHKETVPSYEVLDNQSY g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g a g t a c t c a a c c a a g t c a t t c t g a g a a t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c a t g a g t t g g t t c a g t a a g a c t c t t a t

6480 90 85 80 75 HIRRGLQEQGADIRSLVAGC AmpR A T A A T A C C G C G C C A C A Amp-R HIRRGLQEQGADIRSLVAGC g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g c g t c a a t a c g g g a t a a t a c c g c g c c a c a c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c g c a g t t a t g c c c t a t t a t g g c g c g g t g t

XmnI 6540 70 65 60 55 LLVKFTSMMPFREEPRFSEL AmpR T A G C Amp-R LLVKFTSMMPFREEPRFSEL t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t c t t c g g g g c g a a a a c t c t c a a g a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t t t g c a a g a a g c c c c g c t t t t g a g a g t t c

6600 50 45 40 35 IKGSNLDLEIYGVRAGLQDE AmpR IKGSNLDLEIYGVRAGLQDE g a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t a a c c c a c t c g t g c a c c c a a c t g a t c t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c a t t g g g t g a g c a c g t g g g t t g a c t a g a a g

6660 30 25 ADKVKVLTEPH 20 15 signal sequence AFVPLCFAA AmpR ADKVKVLTEPHAFVPLCFAA a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t g a g c a a a a a c a g g a a g g c a a a a t g c c g c t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a c t c g t t t t t g t c c t t c c g t t t t a c g g c g

SspI 6720 10 5 1 signal sequence FFPILAVRFHQISM AmpR AmpR promoter FFPILAVRFHQISM a a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t t g a a t a c t c a t a c t c t t c c t t t t t c a a t a t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a a c t t a t g a g t a t g a g a a g g a a a a a g t t a t

6780 AmpR promoter t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a t g t a t t t a a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a a c t t a c a t a a a t

SexAI*CsiI 6833 AmpR promoter g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c c t g c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g t g g a c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.