Addgene pVRb17_up1691 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pVRb17_up1691 Sequencing Result

Map View

1000 750 500 250 End (1001) NspI - SphI (996) BfuAI - BspMI (993) PstI - SbfI (990) SfcI (986) SacI - BanII - BsiHKAI (962) Eco53kI (960) BpuEI (937) GFP-F (909 .. 929) DrdI (907) BstBI (877) HincII (860) SalI (858) BtgZI (836) MfeI (815) AclI (764) BstZ17I (705) AvaI XhoI BsoBI PspXI PaeR7I (676) DraI (645) MluI - AflIII (577) PpuMI (566) BsrGI (531) BsaWI - BspEI * (477) MscI (426) EaeI (424) BaeGI Bme1580I (329) GFP-R (286 .. 314) BpmI (302) BseRI (259) BamHI (221) PshAI (191) pBRforEco (118 .. 136) MmeI - BspHI (98) AatII (80) ZraI (78) PvuII - MspA1I (64) PluTI - HaeII (59) SfoI (57) NarI (56) KasI - BanI (55) Start (0) superfolder GFP superfolder GFP pVRb17_up1691 1001 bp

Sequence View

BanIKasINarISfoIHaeIIPluTIStart (0) 5ʹ 3ʹ 60 g g c g g c a a g a a a g c c a t c c a g t t t a c t t t g c a g g g c t t c c c a a c c t t a c c a g a g g g c g c c c c g c c g t t c t t t c g g t a g g t c a a a t g a a a c g t c c c g a a g g g t t g g a a t g g t c t c c c g c g g

BspHIMmeIZraIAatIIMspA1IPvuII 120 A A T pBRforEco c c a g c t g g c a a t t c c g a c g t c t a a g a a a c c a t t a t t a t c a t g a c a t t a a c c t a t a a a a a t g g t c g a c c g t t a a g g c t g c a g a t t c t t t g g t a a t a a t a g t a c t g t a a t t g g a t a t t t t t a

180 A G G C G T A T C A C G A G G C pBRforEco a g g c g t a t c a c g a g g c c c t t c a g t a c a a a a t t t t t t a g a t g c g t t t g g t g a a c c a a a c t c t c c g c a t a g t g c t c c g g g a a g t c a t g t t t t a a a a a a t c t a c g c a a a c c a c t t g g t t t g a g

BamHIPshAI 240 t t a c t c g a c t c g t g t c a g t a a g c g g g a g g t g a t c g c g t g g g g a t c c t g t c a a t t t c c g c g a a t g a g c t g a g c a c a g t c a t t c g c c c t c c a c t a g c g c a c c c c t a g g a c a g t t a a a g g c g c

BseRI 300 1 M 5 10 15 SKGEELFTGVVPIL superfolder GFP 1 5 10 15 MSKGEELFTGVVPIL superfolder GFP C A A C A G G G T T A A G A A GFP-R MSKGEELFTGVVPIL a t a g a g g a g g t a a a g a t g a g c a a a g g a g a a g a a c t t t t c a c t g g a g t t g t c c c a a t t c t t t a t c t c c t c c a t t t c t a c t c g t t t c c t c t t c t t g a a a a g t g a c c t c a a c a g g g t t a a g a a

Bme1580IBaeGIBpmI 360 20 25 30 35 VELDGDVNGHKFSVRGEGEG superfolder GFP 20 25 30 35 VELDGDVNGHKFSVRGEGEG superfolder GFP C A A C T T A A T C T A C C GFP-R VELDGDVNGHKFSVRGEGEG g t t g a a t t a g a t g g t g a t g t t a a t g g g c a c a a a t t t t c t g t c c g t g g a g a g g g t g a a g g t c a a c t t a a t c t a c c a c t a c a a t t a c c c g t g t t t a a a a g a c a g g c a c c t c t c c c a c t t c c a

420 40 45 50 55 DATNGKLTLKFICTTGKLPV superfolder GFP 40 45 50 55 DATNGKLTLKFICTTGKLPV superfolder GFP DATNGKLTLKFICTTGKLPV g a t g c t a c a a a c g g a a a a c t c a c c c t t a a a t t t a t t t g c a c t a c t g g a a a a c t a c c t g t t c t a c g a t g t t t g c c t t t t g a g t g g g a a t t t a a a t a a a c g t g a t g a c c t t t t g a t g g a c a a

BspEI*BsaWIEaeIMscI 480 60 65 70 75 PWPTLVTTLTYGVQCFSRYP superfolder GFP 60 65 70 75 PWPTLVTTLTYGVQCFSRYP superfolder GFP PWPTLVTTLTYGVQCFSRYP c c g t g g c c a a c a c t t g t c a c t a c t c t g a c c t a t g g t g t t c a a t g c t t t t c c c g t t a t c c g g g c a c c g g t t g t g a a c a g t g a t g a g a c t g g a t a c c a c a a g t t a c g a a a a g g g c a a t a g g c

BsrGI 540 80 85 90 95 DHMKRHDFFKSAMPEGYVQE superfolder GFP 80 85 90 95 DHMKRHDFFKSAMPEGYVQE superfolder GFP DHMKRHDFFKSAMPEGYVQE g a t c a c a t g a a a c g g c a t g a c t t t t t c a a g a g t g c c a t g c c c g a a g g t t a t g t a c a g g a a c t a g t g t a c t t t g c c g t a c t g a a a a a g t t c t c a c g g t a c g g g c t t c c a a t a c a t g t c c t t

AflIIIMluIPpuMI 600 100 105 110 115 RTISFKDDGTYKTRAEVKFE superfolder GFP 100 105 110 115 RTISFKDDGTYKTRAEVKFE superfolder GFP RTISFKDDGTYKTRAEVKFE c g c a c t a t a t c t t t c a a a g a t g a c g g g a c c t a c a a g a c g c g t g c t g a a g t c a a g t t t g a a g c g t g a t a t a g a a a g t t t c t a c t g c c c t g g a t g t t c t g c g c a c g a c t t c a g t t c a a a c t t

DraI 660 120 125 130 135 GDTLVNRIELKGIDFKEDGN superfolder GFP 120 125 130 135 GDTLVNRIELKGIDFKEDGN superfolder GFP GDTLVNRIELKGIDFKEDGN g g t g a t a c c c t t g t t a a t c g t a t c g a g t t a a a g g g t a t t g a t t t t a a a g a a g a t g g a a a c c c a c t a t g g g a a c a a t t a g c a t a g c t c a a t t t c c c a t a a c t a a a a t t t c t t c t a c c t t t g

BstZ17IPaeR7IBsoBIPspXIXhoIAvaI 720 140 145 150 155 ILGHKLEYNFNSHNVYITAD superfolder GFP 140 145 150 155 ILGHKLEYNFNSHNVYITAD superfolder GFP ILGHKLEYNFNSHNVYITAD a t t c t t g g a c a c a a a c t c g a g t a c a a c t t t a a c t c a c a c a a t g t a t a c a t c a c g g c a g a c t a a g a a c c t g t g t t t g a g c t c a t g t t g a a a t t g a g t g t g t t a c a t a t g t a g t g c c g t c t g

AclI 780 160 165 170 175 KQKNGIKANFKIRHNVEDGS superfolder GFP 160 165 170 175 KQKNGIKANFKIRHNVEDGS superfolder GFP KQKNGIKANFKIRHNVEDGS a a a c a a a a g a a t g g a a t c a a a g c t a a c t t c a a a a t t c g c c a c a a c g t t g a a g a t g g t t c c t t t g t t t t c t t a c c t t a g t t t c g a t t g a a g t t t t a a g c g g t g t t g c a a c t t c t a c c a a g g

BtgZIMfeI 840 180 185 190 195 VQLADHYQQNTPIGDGPVLL superfolder GFP 180 185 190 195 VQLADHYQQNTPIGDGPVLL superfolder GFP VQLADHYQQNTPIGDGPVLL g t t c a a c t a g c a g a c c a t t a t c a a c a a a a t a c t c c a a t t g g c g a t g g c c c t g t c c t t t t a c a a g t t g a t c g t c t g g t a a t a g t t g t t t t a t g a g g t t a a c c g c t a c c g g g a c a g g a a a a t

BstBISalIHincII 900 200 205 210 215 PDNHYLSTQSVLSKDPNEKR superfolder GFP 200 205 210 215 PDNHYLSTQSVLSKDPNEKR superfolder GFP PDNHYLSTQSVLSKDPNEKR c c a g a c a a c c a t t a c c t g t c g a c a c a a t c t g t c c t t t c g a a a g a t c c c a a c g a a a a g c g t g g t c t g t t g g t a a t g g a c a g c t g t g t t a g a c a g g a a a g c t t t c t a g g g t t g c t t t t c g c a

Eco53kIBpuEIDrdI 960 220 225 230 235 DHMVLLEFVTAAGITHGMDE superfolder GFP 220 225 230 235 DHMVLLEFVTAAGITHGMDE superfolder GFP G G T C C T T C T T G A G T T T G T A A C GFP-F DHMVLLEFVTAAGITHGMDE g a c c a c a t g g t c c t t c t t g a g t t t g t a a c t g c t g c t g g g a t t a c a c a t g g c a t g g a t g a g c t g g t g t a c c a g g a a g a a c t c a a a c a t t g a c g a c g a c c c t a a t g t g t a c c g t a c c t a c t c

End (1001) SfcISbfIPstIBspMIBfuAISphINspIBsiHKAIBanIISacI 3ʹ 5ʹ 1001 LYK* superfolder GFP LYK * superfolder GFP LYK* c t c t a c a a a t a a t a a a t g t c c a g a c c t g c a g g c a t g c a a g c g a g a t g t t t a t t a t t t a c a g g t c t g g a c g t c c g t a c g t t c g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.