Addgene pEGFP-N1-wtVHL - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pEGFP-N1-wtVHL


Map View

AflIII - PciI (5209) pBR322ori-F (5054 .. 5073) ApaLI (4895) BsaI (4280) TK-pA-R (4145 .. 4164) BstBI (3973) Neo-F (3827 .. 3846) RsrII (3807) FspI (3393) MscI (3373) PluTI (3294) SfoI (3292) NarI (3291) KasI (3290) Neo-R (3217 .. 3236) BspDI * - ClaI * (3131) StuI (3112) SfiI (3066) CsiI - SexAI * (2880) pBABE 3' (2794 .. 2814) AseI (7) NdeI (234) SnaBI (340) CMV-F (519 .. 539) NheI (591) BmtI (595) AfeI (596) BglII (609) Eco53kI (618) SacI (620) HindIII (622) SacII (711) BbvCI (817) BstEII (873) KflI - PpuMI (901) AccI (999) BstZ17I (1000) EcoNI (1167) BamHI (1194) AgeI (1200) EGFP-N (1258 .. 1279) EXFP-R (1519 .. 1538) EGFP-C (1866 .. 1887) BsrGI (1922) XbaI * (1945) MfeI (2041) SV40pA-R (2092 .. 2111) EBV-rev (2146 .. 2165) AflII (2173) F1ori-F (2320 .. 2341) DraIII (2407) F1ori-R (2532 .. 2551) pEGFP-N1-wtVHL 5267 bp

Sequence View

AseI 5ʹ 3ʹ 60 t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a t a t g g a g t t c c g a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t a t a c c t c a a g g c

120 CMV enhancer c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c c c c g c c c a t t g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g g g g c g g g t a a

180 CMV enhancer g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c a t t g a c g t c a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g t a a c t g c a g t

NdeI 240 CMV enhancer a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t a t c a t a t g c c t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a t a g t a t a c g g

300 CMV enhancer a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t a t g c c c a g t a t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a t a c g g g t c a t

SnaBI 360 CMV enhancer c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t c g c t a t t a c g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t a g c g a t a a t g

420 CMV enhancer CMV promoter c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t t g a c t c a c g g g g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a a c t g a g t g c c c c

480 CMV promoter a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c a a a a t c a a c g t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g t t t t a g t t g c

540 CMV promoter C G C A A A T G G G C G G T A G G C G T G CMV-F g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g c g g t a g g c g t g t c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c g c c a t c c g c a c a

NheIBmtIAfeI 600 CMV promoter a c g g t g g g a g g t c t a t a t a a g c a g a g c t g g t t t a g t g a a c c g t c a g a t c c g c t a g c g c t a t g c c a c c c t c c a g a t a t a t t c g t c t c g a c c a a a t c a c t t g g c a g t c t a g g c g a t c g c g a t

Eco53kISacIHindIIIBglII 660 MPRKAASP c c g g a c t c a g a t c t c g a g c t c a a g c t t c g g c c a c c a t g c c c c g g a a g g c a g c t a g t c c a g g g c c t g a g t c t a g a g c t c g a g t t c g a a g c c g g t g g t a c g g g g c c t t c c g t c g a t c a g g t c

SacII 720 EEAERMPGSEEIEAGRPRPV a g g a g g c a g a a a g g a t g c c g g g c t c t g a a g a g a t a g a g g c t g g g c g g c c g c g g c c g g t t t t c c t c c g t c t t t c c t a c g g c c c g a g a c t t c t c t a t c t c c g a c c c g c c g g c g c c g g c c a a a

780 LRSVNSREPSQVIFCNRSPR t a c g c t c t g t g a a c t c g c g c g a a c c c t c t c a g g t c a t c t t c t g c a a c c g c a g c c c g c g c g a t g c g a g a c a c t t g a g c g c g c t t g g g a g a g t c c a g t a g a a g a c g t t g g c g t c g g g c g c g c

BbvCI 840 VVLPLWLNFDGEPQPYPTLP t c g t g c t g c c t t t g t g g c t c a a c t t t g a t g g t g a g c c t c a g c c c t a c c c g a c c t t a c c a c a g c a c g a c g g a a a c a c c g a g t t g a a a c t a c c a c t c g g a g t c g g g a t g g g c t g g a a t g g t g

BstEII 900 PGTGRRIHSYRGHLWLFRDA c g g g c a c c g g c c g c c g c a t c c a c a g c t a c c g a g g t c a c c t t t g g c t c t t c a g g g a t g c g g g c c c g t g g c c g g c g g c g t a g g t g t c g a t g g c t c c a g t g g a a a c c g a g a a g t c c c t a c g c c

PpuMIKflI 960 GTHDGLLVNQTELFVPSLNV g g a c c c a t g a t g g a c t t c t g g t t a a c c a a a c g g a a c t g t t t g t g c c a t c c c t c a a t g t t g c c t g g g t a c t a c c t g a a g a c c a a t t g g t t t g c c t t g a c a a a c a c g g t a g g g a g t t a c a a c

AccIBstZ17I 1020 DGQPIFANITLPVYTLKERC a t g g a c a g c c t a t t t t t g c c a a c a t c a c a t t g c c a g t g t a t a c c c t g a a a g a g c g g t g c c t a c c t g t c g g a t a a a a a c g g t t g t a g t g t a a c g g t c a c a t a t g g g a c t t t c t c g c c a c g g

1080 LQVVRSLVKPENYRRLDIVR t t c a g g t t g t a c g g a g c c t g g t c a a g c c t g a g a a c t a c a g g a g g c t g g a c a t c g t c a g g t a a g t c c a a c a t g c c t c g g a c c a g t t c g g a c t c t t g a t g t c c t c c g a c c t g t a g c a g t c c a

1140 SLYEDLEDHPNVRKDIQRLT c g c t c t a t g a a g a c t t g g a a g a c c a c c c a a a t g t g c g g a a a g a c a t a c a g c g g c t g a c c c g c g a g a t a c t t c t g a a c c t t c t g g t g g g t t t a c a c g c c t t t c t g t a t g t c g c c g a c t g g g

BamHIEcoNI 1200 QEHLENQALGEEPEGVH* a a g a g c a c c t c g a g a a t c a g g c c c t g g g a g a g g a g c c t g a a g g a g t c c a c t g a g g a t c c a t t c t c g t g g a g c t c t t a g t c c g g g a c c c t c t c c t c g g a c t t c c t c a g g t g a c t c c t a g g t

AgeI 1260 1 M 1a V 5 10 15 SKGEELFTGVVPIL EGFP 1 M 1a V 5 10 15 SKGEELFTGVVPIL EGFP Kozak sequence G A C EGFP-N MVSKGEELFTGVVPIL c c g g t c g c c a c c a t g g t g a g c a a g g g c g a g g a g c t g t t c a c c g g g g t g g t g c c c a t c c t g g g c c a g c g g t g g t a c c a c t c g t t c c c g c t c c t c g a c a a g t g g c c c c a c c a c g g g t a g g a c

1320 20 25 30 35 VELDGDVNGHKFSVSGEGEG EGFP 20 25 30 35 VELDGDVNGHKFSVSGEGEG EGFP C A G C T C G A C C T G C C G C T G C EGFP-N VELDGDVNGHKFSVSGEGEG g t c g a g c t g g a c g g c g a c g t a a a c g g c c a c a a g t t c a g c g t g t c c g g c g a g g g c g a g g g c c a g c t c g a c c t g c c g c t g c a t t t g c c g g t g t t c a a g t c g c a c a g g c c g c t c c c g c t c c c g

1380 40 45 50 55 DATYGKLTLKFICTTGKLPV EGFP 40 45 50 55 DATYGKLTLKFICTTGKLPV EGFP DATYGKLTLKFICTTGKLPV g a t g c c a c c t a c g g c a a g c t g a c c c t g a a g t t c a t c t g c a c c a c c g g c a a g c t g c c c g t g c t a c g g t g g a t g c c g t t c g a c t g g g a c t t c a a g t a g a c g t g g t g g c c g t t c g a c g g g c a c

1440 60 65 70 75 PWPTLVTTLTYGVQCFSRYP EGFP 60 65 70 75 PWPTLVTTLTYGVQCFSRYP EGFP PWPTLVTTLTYGVQCFSRYP c c c t g g c c c a c c c t c g t g a c c a c c c t g a c c t a c g g c g t g c a g t g c t t c a g c c g c t a c c c c g g g a c c g g g t g g g a g c a c t g g t g g g a c t g g a t g c c g c a c g t c a c g a a g t c g g c g a t g g g g

1500 80 85 90 95 DHMKQHDFFKSAMPEGYVQE EGFP 80 85 90 95 DHMKQHDFFKSAMPEGYVQE EGFP DHMKQHDFFKSAMPEGYVQE g a c c a c a t g a a g c a g c a c g a c t t c t t c a a g t c c g c c a t g c c c g a a g g c t a c g t c c a g g a g c t g g t g t a c t t c g t c g t g c t g a a g a a g t t c a g g c g g t a c g g g c t t c c g a t g c a g g t c c t c

1560 100 105 110 115 RTIFFKDDGNYKTRAEVKFE EGFP 100 105 110 115 RTIFFKDDGNYKTRAEVKFE EGFP C T G C T G C C G T T G A T G T T C T G EXFP-R RTIFFKDDGNYKTRAEVKFE c g c a c c a t c t t c t t c a a g g a c g a c g g c a a c t a c a a g a c c c g c g c c g a g g t g a a g t t c g a g g c g t g g t a g a a g a a g t t c c t g c t g c c g t t g a t g t t c t g g g c g c g g c t c c a c t t c a a g c t c

1620 120 125 130 135 GDTLVNRIELKGIDFKEDGN EGFP 120 125 130 135 GDTLVNRIELKGIDFKEDGN EGFP GDTLVNRIELKGIDFKEDGN g g c g a c a c c c t g g t g a a c c g c a t c g a g c t g a a g g g c a t c g a c t t c a a g g a g g a c g g c a a c c c g c t g t g g g a c c a c t t g g c g t a g c t c g a c t t c c c g t a g c t g a a g t t c c t c c t g c c g t t g

1680 140 145 150 155 ILGHKLEYNYNSHNVYIMAD EGFP 140 145 150 155 ILGHKLEYNYNSHNVYIMAD EGFP ILGHKLEYNYNSHNVYIMAD a t c c t g g g g c a c a a g c t g g a g t a c a a c t a c a a c a g c c a c a a c g t c t a t a t c a t g g c c g a c t a g g a c c c c g t g t t c g a c c t c a t g t t g a t g t t g t c g g t g t t g c a g a t a t a g t a c c g g c t g

1740 160 165 170 175 KQKNGIKVNFKIRHNIEDGS EGFP 160 165 170 175 KQKNGIKVNFKIRHNIEDGS EGFP KQKNGIKVNFKIRHNIEDGS a a g c a g a a g a a c g g c a t c a a g g t g a a c t t c a a g a t c c g c c a c a a c a t c g a g g a c g g c a g c t t c g t c t t c t t g c c g t a g t t c c a c t t g a a g t t c t a g g c g g t g t t g t a g c t c c t g c c g t c g

1800 180 185 190 195 VQLADHYQQNTPIGDGPVLL EGFP 180 185 190 195 VQLADHYQQNTPIGDGPVLL EGFP VQLADHYQQNTPIGDGPVLL g t g c a g c t c g c c g a c c a c t a c c a g c a g a a c a c c c c c a t c g g c g a c g g c c c c g t g c t g c t g c a c g t c g a g c g g c t g g t g a t g g t c g t c t t g t g g g g g t a g c c g c t g c c g g g g c a c g a c g a c

1860 200 205 210 215 PDNHYLSTQSALSKDPNEKR EGFP 200 205 210 215 PDNHYLSTQSALSKDPNEKR EGFP PDNHYLSTQSALSKDPNEKR c c c g a c a a c c a c t a c c t g a g c a c c c a g t c c g c c c t g a g c a a a g a c c c c a a c g a g a a g c g c g g g c t g t t g g t g a t g g a c t c g t g g g t c a g g c g g g a c t c g t t t c t g g g g t t g c t c t t c g c g

1920 220 225 230 235 DHMVLLEFVTAAGITLGMDE EGFP 220 225 230 235 DHMVLLEFVTAAGITLGMDE EGFP C A T G G T C C T G C T G G A G T T C G T G EGFP-C DHMVLLEFVTAAGITLGMDE g a t c a c a t g g t c c t g c t g g a g t t c g t g a c c g c c g c c g g g a t c a c t c t c g g c a t g g a c g a g c t a g t g t a c c a g g a c g a c c t c a a g c a c t g g c g g c g g c c c t a g t g a g a g c c g t a c c t g c t c

XbaI*BsrGI 1980 LYK* EGFP LYK * EGFP LYK* c t g t a c a a g t a a a g c g g c c g c g a c t c t a g a t c a t a a t c a g c c a t a c c a c a t t t g t a g a g g g a c a t g t t c a t t t c g c c g g c g c t g a g a t c t a g t a t t a g t c g g t a t g g t g t a a a c a t c t c c

2040 t t t t a c t t g c t t t a a a a a a c c t c c c a c a c c t c c c c c t g a a c c t g a a a c a t a a a a t g a a t g a a a a t g a a c g a a a t t t t t t g g a g g g t g t g g a g g g g g a c t t g g a c t t t g t a t t t t a c t t a c

MfeI 2100 SV40 poly(A) signal C G T T A T C G T SV40pA-R c a a t t g t t g t t g t t a a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c a a t a g c a g t t a a c a a c a a c a a t t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g t t a t c g t

2160 SV40 poly(A) signal G T G G T T T G T C C A A A C EBV-rev A G T G T T T A A A G SV40pA-R t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t c c a a a c a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c a g g t t t g

AflII 2220 SV40 poly(A) signal f1 ori T C A T C EBV-rev t c a t c a a t g t a t c t t a a g g c g t a a a t t g t a a g c g t t a a t a t t t t g t t a a a a t t c g c g t t a a g t a g t t a c a t a g a a t t c c g c a t t t a a c a t t c g c a a t t a t a a a a c a a t t t t a a g c g c a a t

2280 f1 ori a a t t t t t g t t a a a t c a g c t c a t t t t t t a a c c a a t a g g c c g a a a t c g g c a a a a t c c c t t a t t t a a a a a c a a t t t a g t c g a g t a a a a a a t t g g t t a t c c g g c t t t a g c c g t t t t a g g g a a t a

2340 f1 ori G G T C A A A C C T T G T T C T C A G G T F1ori-F a a a t c a a a a g a a t a g a c c g a g a t a g g g t t g a g t g t t g t t c c a g t t t g g a a c a a g a g t c c a t t t a g t t t t c t t a t c t g g c t c t a t c c c a a c t c a c a a c a a g g t c a a a c c t t g t t c t c a g g t

2400 f1 ori G F1ori-F c t a t t a a a g a a c g t g g a c t c c a a c g t c a a a g g g c g a a a a a c c g t c t a t c a g g g c g a t g g c g a t a a t t t c t t g c a c c t g a g g t t g c a g t t t c c c g c t t t t t g g c a g a t a g t c c c g c t a c c g

DraIII 2460 f1 ori c c a c t a c g t g a a c c a t c a c c c t a a t c a a g t t t t t t g g g g t c g a g g t g c c g t a a a g c a c t a g g t g a t g c a c t t g g t a g t g g g a t t a g t t c a a a a a a c c c c a g c t c c a c g g c a t t t c g t g a t

2520 f1 ori a a t c g g a a c c c t a a a g g g a g c c c c c g a t t t a g a g c t t g a c g g g g a a a g c c g g c g a a c g t g t t a g c c t t g g g a t t t c c c t c g g g g g c t a a a t c t c g a a c t g c c c c t t t c g g c c g c t t g c a c

2580 f1 ori A G G G A A G A A A G C G A A A G G A G F1ori-R g c g a g a a a g g a a g g g a a g a a a g c g a a a g g a g c g g g c g c t a g g g c g c t g g c a a g t g t a g c g c g c t c t t t c c t t c c c t t c t t t c g c t t t c c t c g c c c g c g a t c c c g c g a c c g t t c a c a t c g c

2640 f1 ori g t c a c g c t g c g c g t a a c c a c c a c a c c c g c c g c g c t t a a t g c g c c g c t a c a g g g c g c g t c a c a g t g c g a c g c g c a t t g g t g g t g t g g g c g g c g c g a a t t a c g c g g c g a t g t c c c g c g c a g t

2700 AmpR promoter g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a

2760 AmpR promoter t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t

2820 AmpR promoter SV40 promoter SV40 enhancer C C T T A C A C A C A G T C A A T C C C A pBABE 3' a g g a a g a g t c c t g a g g c g g a a a g a a c c a g c t g t g g a a t g t g t g t c a g t t a g g g t g t g g a a t c c t t c t c a g g a c t c c g c c t t t c t t g g t c g a c a c c t t a c a c a c a g t c a a t c c c a c a c c t t

2880 SV40 promoter SV40 enhancer a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t

SexAI*CsiI 2940 SV40 promoter SV40 enhancer c c a g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a g g t c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t

3000 SV40 promoter SV40 enhancer SV40 ori a t t a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c c a t a a t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g g t

3060 SV40 promoter SV40 ori g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c

StuISfiI 3120 SV40 promoter SV40 ori c c g c c t c g g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g c t g g c g g a g c c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c g a

BspDI*ClaI* 3180 1 5 MIEQDG SV40 promoter NeoR/KanR 1 5 MIEQDG NeoR/KanR MIEQDG t t t g c a a a g a t c g a t c a a g a g a c a g g a t g a g g a t c g t t t c g c a t g a t t g a a c a a g a t g g a a a a c g t t t c t a g c t a g t t c t c t g t c c t a c t c c t a g c a a a g c g t a c t a a c t t g t t c t a c c t

3240 10 15 20 25 LHAGSPAAWVERLFGYDWAQ NeoR/KanR 10 15 20 25 LHAGSPAAWVERLFGYDWAQ NeoR/KanR G A T A A G C C G A T A C T G A C C C G Neo-R LHAGSPAAWVERLFGYDWAQ t t g c a c g c a g g t t c t c c g g c c g c t t g g g t g g a g a g g c t a t t c g g c t a t g a c t g g g c a c a a a a c g t g c g t c c a a g a g g c c g g c g a a c c c a c c t c t c c g a t a a g c c g a t a c t g a c c c g t g t t

KasINarISfoIPluTI 3300 30 35 40 45 QTIGCSDAAVFRLSAQGRPV NeoR/KanR 30 35 40 45 QTIGCSDAAVFRLSAQGRPV NeoR/KanR QTIGCSDAAVFRLSAQGRPV c a g a c a a t c g g c t g c t c t g a t g c c g c c g t g t t c c g g c t g t c a g c g c a g g g g c g c c c g g t t g t c t g t t a g c c g a c g a g a c t a c g g c g g c a c a a g g c c g a c a g t c g c g t c c c c g c g g g c c a a

3360 50 55 60 65 LFVKTDLSGALNELQDEAAR NeoR/KanR 50 55 60 65 LFVKTDLSGALNELQDEAAR NeoR/KanR LFVKTDLSGALNELQDEAAR c t t t t t g t c a a g a c c g a c c t g t c c g g t g c c c t g a a t g a a c t g c a a g a c g a g g c a g c g c g g g a a a a a c a g t t c t g g c t g g a c a g g c c a c g g g a c t t a c t t g a c g t t c t g c t c c g t c g c g c c

FspIMscI 3420 70 75 80 85 LSWLATTGVPCAAVLDVVTE NeoR/KanR 70 75 80 85 LSWLATTGVPCAAVLDVVTE NeoR/KanR LSWLATTGVPCAAVLDVVTE c t a t c g t g g c t g g c c a c g a c g g g c g t t c c t t g c g c a g c t g t g c t c g a c g t t g t c a c t g a a g a t a g c a c c g a c c g g t g c t g c c c g c a a g g a a c g c g t c g a c a c g a g c t g c a a c a g t g a c t t

3480 90 95 100 105 AGRDWLLLGEVPGQDLLSSH NeoR/KanR 90 95 100 105 AGRDWLLLGEVPGQDLLSSH NeoR/KanR AGRDWLLLGEVPGQDLLSSH *RV g c g g g a a g g g a c t g g c t g c t a t t g g g c g a a g t g c c g g g g c a g g a t c t c c t g t c a t c t c a c c g c c c t t c c c t g a c c g a c g a t a a c c c g c t t c a c g g c c c c g t c c t a g a g g a c a g t a g a g t g

3540 110 115 120 125 LAPAEKVSIMADAMRRLHTL NeoR/KanR 110 115 120 125 LAPAEKVSIMADAMRRLHTL NeoR/KanR LAPAEKVSIMADAMRRLHTL KSRGLFYGDHSICHPPQMRK c t t g c t c c t g c c g a g a a a g t a t c c a t c a t g g c t g a t g c a a t g c g g c g g c t g c a t a c g c t t g a a c g a g g a c g g c t c t t t c a t a g g t a g t a c c g a c t a c g t t a c g c c g c c g a c g t a t g c g a a

3600 130 135 140 145 DPATCPFDHQAKHRIERART NeoR/KanR 130 135 140 145 DPATCPFDHQAKHRIERART NeoR/KanR DPATCPFDHQAKHRIERART IRSGAWEVVLRFMADLSCTS g a t c c g g c t a c c t g c c c a t t c g a c c a c c a a g c g a a a c a t c g c a t c g a g c g a g c a c g t a c t c t a g g c c g a t g g a c g g g t a a g c t g g t g g t t c g c t t t g t a g c g t a g c t c g c t c g t g c a t g a

3660 150 155 160 165 RMEAGLVDQDDLDEEHQGLA NeoR/KanR 150 155 160 165 RMEAGLVDQDDLDEEHQGLA NeoR/KanR RMEAGLVDQDDLDEEHQGLA PHFGTKDILIIQVFLMLPER c g g a t g g a a g c c g g t c t t g t c g a t c a g g a t g a t c t g g a c g a a g a g c a t c a g g g g c t c g c g g c c t a c c t t c g g c c a g a a c a g c t a g t c c t a c t a g a c c t g c t t c t c g t a g t c c c c g a g c g c

3720 170 175 180 185 PAELFARLKASMPDGEDLVV NeoR/KanR 170 175 180 185 PAELFARLKASMPDGEDLVV NeoR/KanR PAELFARLKASMPDGEDLVV WGFQEGPELRAHGVALIEDH c c a g c c g a a c t g t t c g c c a g g c t c a a g g c g a g c a t g c c c g a c g g c g a g g a t c t c g t c g t g g g t c g g c t t g a c a a g c g g t c c g a g t t c c g c t c g t a c g g g c t g c c g c t c c t a g a g c a g c a c

3780 190 195 200 205 THGDACLPNIMVENGRFSGF NeoR/KanR 190 195 200 205 THGDACLPNIMVENGRFSGF NeoR/KanR THGDACLPNIMVENGRFSGF GMAIGAQRIDHHFIAAKRSE a c c c a t g g c g a t g c c t g c t t g c c g a a t a t c a t g g t g g a a a a t g g c c g c t t t t c t g g a t t c t g g g t a c c g c t a c g g a c g a a c g g c t t a t a g t a c c a c c t t t t a c c g g c g a a a a g a c c t a a g

RsrII 3840 210 215 220 225 IDCGRLGVADRYQDIALATR NeoR/KanR 210 215 220 225 IDCGRLGVADRYQDIALATR NeoR/KanR C G T T G G C T A C C C G T Neo-F IDCGRLGVADRYQDIALATR DVTAPQTHRVAILVYRQSGT a t c g a c t g t g g c c g g c t g g g t g t g g c g g a c c g c t a t c a g g a c a t a g c g t t g g c t a c c c g t t a g c t g a c a c c g g c c g a c c c a c a c c g c c t g g c g a t a g t c c t g t a t c g c a a c c g a t g g g c a

3900 230 235 240 245 DIAEELGGEWADRFLVLYGI NeoR/KanR 230 235 240 245 DIAEELGGEWADRFLVLYGI NeoR/KanR G A T A T T Neo-F DIAEELGGEWADRFLVLYGI INSFLKAAFPSVAEEHKVTD g a t a t t g c t g a a g a g c t t g g c g g c g a a t g g g c t g a c c g c t t c c t c g t g c t t t a c g g t a t c c t a t a a c g a c t t c t c g a a c c g c c g c t t a c c c g a c t g g c g a a g g a g c a c g a a a t g c c a t a g

3960 250 255 260 265 AAPDSQRIAFYRLLDEFF* NeoR/KanR 250 255 260 265 AAPDSQRIAFYRLLDEFF* NeoR/KanR AAPDSQRIAFYRLLDEFF* GSGIRLADGEIAKKVLEESR g c c g c t c c c g a t t c g c a g c g c a t c g c c t t c t a t c g c c t t c t t g a c g a g t t c t t c t g a g c g c g g c g a g g g c t a a g c g t c g c g t a g c g g a a g a t a g c g g a a g a a c t g c t c a a g a a g a c t c g c

BstBI 4020 MTDQATPNLPSRDF SEPTRFSRGLSAWGAM g g a c t c t g g g g t t c g a a a t g a c c g a c c a a g c g a c g c c c a a c c t g c c a t c a c g a g a t t t c g c c t g a g a c c c c a a g c t t t a c t g g c t g g t t c g c t g c g g g t t g g a c g g t a g t g c t c t a a a g c

4080 DSTAAFYERLGFGIVFRDAG a t t c c a c c g c c g c c t t c t a t g a a a g g t t g g g c t t c g g a a t c g t t t t c c g g g a c g c c g g c t t a a g g t g g c g g c g g a a g a t a c t t t c c a a c c c g a a g c c t t a g c a a a a g g c c c t g c g g c c g a

4140 WMILQRGDLMLEFFAHPRGR g g a t g a t c c t c c a g c g c g g g g a t c t c a t g c t g g a g t t c t t c g c c c a c c c t a g g g g g a g g c c c t a c t a g g a g g t c g c g c c c c t a g a g t a c g a c c t c a a g a a g c g g g t g g g a t c c c c c t c c g

4200 HSV TK poly(A) signal A C T T T G T G C C T T C C T C T G T T TK-pA-R LTETRKETIPEGTRAMTAIK t a a c t g a a a c a c g g a a g g a g a c a a t a c c g g a a g g a a c c c g c g c t a t g a c g g c a a t a a a a a a t t g a c t t t g t g c c t t c c t c t g t t a t g g c c t t c c t t g g g c g c g a t a c t g c c g t t a t t t t t

4260 HSV TK poly(A) signal RQNKTHGVGSFVHKRGVRSQ g a c a g a a t a a a a c g c a c g g t g t t g g g t c g t t t g t t c a t a a a c g c g g g g t t c g g t c c c a g g c t g t c t t a t t t t g c g t g c c a c a a c c c a g c a a a c a a g t a t t t g c g c c c c a a g c c a g g g t c c

BsaI 4320 GWHSVDTPPRPHWGQYARVS g c t g g c a c t c t g t c g a t a c c c c a c c g a g a c c c c a t t g g g g c c a a t a c g c c c g c g t t t c t t c g a c c g t g a g a c a g c t a t g g g g t g g c t c t g g g g t a a c c c c g g t t a t g c g g g c g c a a a g a a

4380 SFSPPHPPSSGEGPGLAANV c c t t t t c c c c a c c c c a c c c c c c a a g t t c g g g t g a a g g c c c a g g g c t c g c a g c c a a c g t c g g g a a a a g g g g t g g g g t g g g g g g t t c a a g c c c a c t t c c g g g t c c c g a g c g t c g g t t g c a g c

4440 GAAGPAIASGYSYIL* g g g c g g c a g g c c c t g c c a t a g c c t c a g g t t a c t c a t a t a t a c t t t a g a t t g a t t t a a a a c c c c g c c g t c c g g g a c g g t a t c g g a g t c c a a t g a g t a t a t a t g a a a t c t a a c t a a a t t t t g

4500 t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a a a a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t t t

4560 t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a

4620 ori c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g

4680 ori t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c

4740 ori g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t c c t t c t a g t g t a g c c g t a g t t a g g c c a c c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a g g a a g a t c a c a t c g g c a t c a a t c c g g t g g

4800 ori a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c

4860 ori c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c

ApaLI 4920 ori a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t

4980 ori c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c

5040 ori a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t

5100 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a

5160 ori g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t

AflIIIPciI 5220 g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g

3ʹ 5ʹ 5267 c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c a t g c a t g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g t a c g t a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.