Addgene pEMS2050 - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pEMS2050


Map View

lac operator M13/pUC Reverse (6039 .. 6061) CAP binding site BspQI - SapI (5845) NspI (5732) PciI (5728) pBR322ori-F (5573 .. 5592) PspFI (5428) BseYI (5424) ScaI (4359) Amp-R (4271 .. 4290) XmnI (4240) F1ori-F (3764 .. 3785) DraIII (3700) F1ori-R (3554 .. 3573) M13/pUC Forward (3318 .. 3340) M13 Forward (3309 .. 3326) BsaBI * (3100) MfeI (3001) M13 Reverse (6058 .. 6074) EcoRI (191) AvrII (197) FseI (206) MluI (209) SpeI (230) NdeI (465) PspOMI (1071) ApaI (1075) SacII (1242) BlpI (1366) PaqCI (1436) MreI - SgrAI (1523) BstAPI (1557) pCAGGS-5 (1806 .. 1828) XbaI (1835) AscI (1937) BbsI (2041) pMT2-F (2085 .. 2104) EGFP-N (2174 .. 2195) EXFP-R (2435 .. 2454) EGFP-C (2782 .. 2803) BsrGI (2838) AsiSI (2859) EBV-rev (2882 .. 2901) SV40pA-R (2936 .. 2955) HpaI (2992) pEMS2050 6100 bp

Sequence View

60 AAV2 ITR c t g c g c g c t c g c t c g c t c a c t g a g g c c g c c c g g g c a a a g c c c g g g c g t c g g g c g a c c t t t g a c g c g c g a g c g a g c g a g t g a c t c c g g c g g g c c c g t t t c g g g c c c g c a g c c c g c t g g a a a

120 AAV2 ITR g g t c g c c c g g c c t c a g t g a g c g a g c g a g c g c g c a g a g a g g g a g t g g c c a a c t c c a t c a c t c c a g c g g g c c g g a g t c a c t c g c t c g c t c g c g c g t c t c t c c c t c a c c g g t t g a g g t a g t g a

180 AAV2 ITR a g g g g t t c c t t g t a g t t a a t g a t t a a c c c g c c a t g c t a c t t a t c t a c g t a g c c a t g c t c t t c c c c a a g g a a c a t c a a t t a c t a a t t g g g c g g t a c g a t g a a t a g a t g c a t c g g t a c g a g a

SpeIFseIMluIAvrIIEcoRI 240 CMV enhancer a g g a a g a t c g g a a t t c c c t a g g c c g g c c a c g c g t t g a c a t t g a t t a t t g a c t a g t t a t t a t c c t t c t a g c c t t a a g g g a t c c g g c c g g t g c g c a a c t g t a a c t a a t a a c t g a t c a a t a a t

300 CMV enhancer a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a t a t g g a g t t c c g c g t t a c a t a t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t a t a c c t c a a g g c g c a a t g t a t

360 CMV enhancer a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c c c c g c c c a t t g a c g t c a a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g g g g c g g g t a a c t g c a g t t a

420 CMV enhancer a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c a t t g a c g t c a a t g g g t g g a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g t a a c t g c a g t t a c c c a c c t

NdeI 480 CMV enhancer MPSTP c t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t a t c a t a t g c c a a g t a c g c c g a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a t a g t a t a c g g t t c a t g c g g

540 CMV enhancer PIDVNDGKWPAWHYAQYMTL c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t a t g c c c a g t a c a t g a c c t t g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a t a c g g g t c a t g t a c t g g a a

600 CMV enhancer chicken β- actin promoter WDFPTWQYIYVLVIAITMGR a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t c g c t a t t a c c a t g g g t c g t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t a g c g a t a a t g g t a c c c a g c

660 chicken β- actin promoter GEPHVLLHSPHLPPLPTPNF a g g t g a g c c c c a c g t t c t g c t t c a c t c t c c c c a t c t c c c c c c c c t c c c c a c c c c c a a t t t t c c a c t c g g g g t g c a a g a c g a a g t g a g a g g g g t a g a g g g g g g g g a g g g g t g g g g g t t a a a

720 chicken β- actin promoter VFIYFLIILCSDGGGGGGGA t g t a t t t a t t t a t t t t t t a a t t a t t t t g t g c a g c g a t g g g g g c g g g g g g g g g g g g g g c g c a c a t a a a t a a a t a a a a a a t t a a t a a a a c a c g t c g c t a c c c c c g c c c c c c c c c c c c c c g c g

780 chicken β- actin promoter RQAGRGGARGGAGRGGEVRR g c g c c a g g c g g g g c g g g g c g g g g c g a g g g g c g g g g c g g g g c g a g g c g g a g a g g t g c g g c g c g c g g t c c g c c c c g c c c c g c c c c g c t c c c c g c c c c g c c c c g c t c c g c c t c t c c a c g c c g c

840 chicken β- actin promoter QPIRAARSESFLLWRGGGGG g c a g c c a a t c a g a g c g g c g c g c t c c g a a a g t t t c c t t t t a t g g c g a g g c g g c g g c g g c g g c g t c g g t t a g t c t c g c c g c g c g a g g c t t t c a a a g g a a a a t a c c g c t c c g c c g c c g c c g c c

900 chicken β- actin promoter chimeric intron GPIKSEARGGRESLRCLRPV c g g c c c t a t a a a a a g c g a a g c g c g c g g c g g g c g g g a g t c g c t g c g t t g c c t t c g c c c c g t g c c g g g a t a t t t t t c g c t t c g c g c g c c g c c c g c c c t c a g c g a c g c a a c g g a a g c g g g g c a

960 chimeric intron PRSAPPRAARPGSD* g c c c c g c t c c g c g c c g c c t c g c g c c g c c c g c c c c g g c t c t g a c t g a c c g c g t t a c t c c c a c g g g g c g a g g c g c g g c g g a g c g c g g c g g g c g g g g c c g a g a c t g a c t g g c g c a a t g a g g g t

1020 chimeric intron *H c a g g t g a g c g g g c g g g a c g g c c c t t c t c c t c c g g g c t g t a a t t a g c g c t t g g t t t a a t g a g t c c a c t c g c c c g c c c t g c c g g g a a g a g g a g g c c c g a c a t t a a t c g c g a a c c a a a t t a c t

PspOMIApaI 1080 chimeric intron RSTEKRHSRSLRLPSRSPGK *LAGPPGKT c g g c t c g t t t c t t t t c t g t g g c t g c g t g a a a g c c t t a a a g g g c t c c g g g a g g g c c c t t t g g c c g a g c a a a g a a a a g a c a c c g a c g c a c t t t c g g a a t t t c c c g a g g c c c t c c c g g g a a a c

1140 chimeric intron HPPSRSPPHTRTHTRPSRRT RPPAARPTRAHTHAHPAGRA t g c g g g g g g g a g c g g c t c g g g g g g t g c g t g c g t g t g t g t g t g c g t g g g g a g c g c c g c g t g a c g c c c c c c c t c g c c g a g c c c c c c a c g c a c g c a c a c a c a c a c g c a c c c c t c g c g g c g c a c

1200 chimeric intron RGASGPPQSRQPRPAPSQAS ARAARRSHASRARRPAKHAG c g g c c c g c g c t g c c c g g c g g c t g t g a g c g c t g c g g g c g c g g c g c g g g g c t t t g t g c g c t c g c c g g g c g c g a c g g g c c g c c g a c a c t c g c g a c g c c c g c g c c g c g c c c c g a a a c a c g c g a g

SacII 1260 chimeric intron RTHALPARGPATGRPAPPSR RTRSPLAAPPPAGRHPPAAL c g c g t g t g c g c g a g g g g a g c g c g g c c g g g g g c g g t g c c c c g c g g t g c g g g g g g g c t g c g a g c g c a c a c g c g c t c c c c t c g c g c c g g c c c c c g c c a c g g g g c g c c a c g c c c c c c c g a c g c t

1320 chimeric intron PSCLSRAPHTRPPTLLPHPR PVFAAHPTHAHPPSCPTHAR g g g g a a c a a a g g c t g c g t g c g g g g t g t g t g c g t g g g g g g g t g a g c a g g g g g t g t g g g c g c c c c c t t g t t t c c g a c g c a c g c c c c a c a c a c g c a c c c c c c c a c t c g t c c c c c a c a c c c g c g

BlpI 1380 chimeric intron PPRATVGGQVGRGSNSLVAR RDPQLGGRCGGGRTASCPGA g g c g g t c g g g c t g t a a c c c c c c c c t g c a c c c c c c t c c c c g a g t t g c t g a g c a c g g c c c g g c c g c c a g c c c g a c a t t g g g g g g g g a c g t g g g g g g a g g g g c t c a a c g a c t c g t g c c g g g c c

PaqCI 1440 chimeric intron SRTRPETRPTARPEGHRAPP EPAPSRAPRPAPSATGPPPH c t t c g g g t g c g g g g c t c c g t g c g g g g c g t g g c g c g g g g c t c g c c g t g c c g g g c g g g g g g t g a a g c c c a c g c c c c g a g g c a c g c c c c g c a c c g c g c c c c g a g c g g c a c g g c c c g c c c c c c a

1500 chimeric intron PPLHPHRAPRPRRPGPLARP RCTPTGPPAPGGRAPSPEPS g g c g g c a g g t g g g g g t g c c g g g c g g g g c g g g g c c g c c t c g g g c c g g g g a g g g c t c g g g g g c c g c c g t c c a c c c c c a c g g c c c g c c c c g c c c c g g c g g a g c c c g g c c c c t c c c g a g c c c c c

BstAPISgrAIMreI 1560 chimeric intron LPAAAGSRRRSDLRPSGCGN PRPPGPAGAATSARRAAAM a g g g g c g c g g c g g c c c c g g a g c g c c g g c g g c t g t c g a g g c g c g g c g a g c c g c a g c c a t t g t c c c c g c g c c g c c g g g g c c t c g c g g c c g c c g a c a g c t c c g c g c c g c t c g g c g t c g g t a a c

1620 chimeric intron GKITITRSPAPVEKTGFRAS c c t t t t a t g g t a a t c g t g c g a g a g g g c g c a g g g a c t t c c t t t g t c c c a a a t c t g g c g g a g g g a a a a t a c c a t t a g c a c g c t c t c c c g c g t c c c t g a a g g a a a c a g g g t t t a g a c c g c c t c

1680 chimeric intron GFDPLRRRVGELPRPRLPAA c c g a a a t c t g g g a g g c g c c g c c g c a c c c c c t c t a g c g g g c g c g g g c g a a g c g g t g c g g c g g g c t t t a g a c c c t c c g c g g c g g c g t g g g g g a g a t c g c c c g c g c c c g c t t c g c c a c g c c g c

1740 chimeric intron GAPLFHAPLAKTRRRAATGK c c g g c a g g a a g g a a a t g g g c g g g g a g g g c c t t c g t g c g t c g c c g c g c c g c c g t c c c c t t c g g c c g t c c t t c c t t t a c c c g c c c c t c c c g g a a g c a c g c a g c g g c g c g g c g g c a g g g g a a g

1800 chimeric intron EM t c c a t c t c c a g c c t c g g g g c t g c c g c a g g g g g a c g g c t g c c t t c g g g g g g g a c g g g g c a g a g g t a g a g g t c g g a g c c c c g a c g g c g t c c c c c t g c c g a c g g a a g c c c c c c c t g c c c c g t c

XbaI 1860 chimeric intron G G T T C G G C T T C T G G C G T G T G A C C pCAGGS-5 g g c g g g g t t c g g c t t c t g g c g t g t g a c c g g c g g c t c t a g a g c c t c t g c t a a c c a t g t t c a c c g c c c c a a g c c g a a g a c c g c a c a c t g g c c g c c g a g a t c t c g g a g a c g a t t g g t a c a a g t

1920 chimeric intron t g c c t t c t t c t t t t t c c t a c a g c t c c t g g g c a a c g t g c t g g t t g t t g t g c t g t c t c a t c a a c g g a a g a a g a a a a a g g a t g t c g a g g a c c c g t t g c a c g a c c a a c a a c a c g a c a g a g t a g t

AscI 1980 chimeric intron t t t t g g c a a a g a a t t g g c g c g c c g a a g t t g g t c g t g a g g c a c t g g g c a g g t a a g t a t c a a a a a a c c g t t t c t t a a c c g c g c g g c t t c a a c c a g c a c t c c g t g a c c c g t c c a t t c a t a g t t

2040 chimeric intron g g t t a c a a g a c a g g t t t a a g g a g a c c a a t a g a a a c t g g g c t t g t c g a g a c a g a g a a g a c t c c a a t g t t c t g t c c a a a t t c c t c t g g t t a t c t t t g a c c c g a a c a g c t c t g t c t c t t c t g a

BbsI 2100 chimeric intron T T G C C T T T C T C T C C A C pMT2-F c t t g c g t t t c t g a t a g g c a c c t a t t g g t c t t a c t g a c a t c c a c t t t g c c t t t c t c t c c a c g a a c g c a a a g a c t a t c c g t g g a t a a c c a g a a t g a c t g t a g g t g a a a c g g a a a g a g a g g t g

2160 1 M 1a V 5 10 SKGEELFTG chimeric intron EmGFP Kozak sequence A G G T pMT2-F MVSKGEELFTG a g g t g t c c a c t c c c a g g c g g c c g c c a c c a t g g t g a g c a a g g g c g a g g a g c t g t t c a c c g g t c c a c a g g t g a g g g t c c g c c g g c g g t g g t a c c a c t c g t t c c c g c t c c t c g a c a a g t g g c c

2220 15 20 25 30 VVPILVELDGDVNGHKFSVS EmGFP G A C C A G C T C G A C C T G C C G C T G C EGFP-N VVPILVELDGDVNGHKFSVS g g t g g t g c c c a t c c t g g t c g a g c t g g a c g g c g a c g t a a a c g g c c a c a a g t t c a g c g t g t c c c a c c a c g g g t a g g a c c a g c t c g a c c t g c c g c t g c a t t t g c c g g t g t t c a a g t c g c a c a g

2280 35 40 45 50 GEGEGDATYGKLTLKFICTT EmGFP GEGEGDATYGKLTLKFICTT c g g c g a g g g c g a g g g c g a t g c c a c c t a c g g c a a g c t g a c c c t g a a g t t c a t c t g c a c c a c g c c g c t c c c g c t c c c g c t a c g g t g g a t g c c g t t c g a c t g g g a c t t c a a g t a g a c g t g g t g

2340 55 60 65 70 GKLPVPWPTLVTTFTYGVQC EmGFP GKLPVPWPTLVTTFTYGVQC c g g c a a g c t g c c c g t g c c c t g g c c c a c c c t c g t g a c c a c c t t c a c c t a c g g c g t g c a g t g g c c g t t c g a c g g g c a c g g g a c c g g g t g g g a g c a c t g g t g g a a g t g g a t g c c g c a c g t c a c

2400 75 80 85 90 FARYPDHMKQHDFFKSAMPE EmGFP FARYPDHMKQHDFFKSAMPE c t t c g c c c g c t a c c c c g a c c a c a t g a a g c a g c a c g a c t t c t t c a a g t c c g c c a t g c c c g a g a a g c g g g c g a t g g g g c t g g t g t a c t t c g t c g t g c t g a a g a a g t t c a g g c g g t a c g g g c t

2460 95 100 105 110 GYVQERTIFFKDDGNYKTRA EmGFP C T G C T G C C G T T G A T G T T C T G EXFP-R GYVQERTIFFKDDGNYKTRA a g g c t a c g t c c a g g a g c g c a c c a t c t t c t t c a a g g a c g a c g g c a a c t a c a a g a c c c g c g c t c c g a t g c a g g t c c t c g c g t g g t a g a a g a a g t t c c t g c t g c c g t t g a t g t t c t g g g c g c g

2520 115 120 125 130 EVKFEGDTLVNRIELKGIDF EmGFP EVKFEGDTLVNRIELKGIDF c g a g g t g a a g t t c g a g g g c g a c a c c c t g g t g a a c c g c a t c g a g c t g a a g g g c a t c g a c t t g c t c c a c t t c a a g c t c c c g c t g t g g g a c c a c t t g g c g t a g c t c g a c t t c c c g t a g c t g a a

2580 135 140 145 150 KEDGNILGHKLEYNYNSHKV EmGFP KEDGNILGHKLEYNYNSHKV c a a g g a g g a c g g c a a c a t c c t g g g g c a c a a g c t g g a g t a c a a c t a c a a c a g c c a c a a g g t g t t c c t c c t g c c g t t g t a g g a c c c c g t g t t c g a c c t c a t g t t g a t g t t g t c g g t g t t c c a

2640 155 160 165 170 YITADKQKNGIKVNFKTRHN EmGFP YITADKQKNGIKVNFKTRHN c t a t a t c a c c g c c g a c a a g c a g a a g a a c g g c a t c a a g g t g a a c t t c a a g a c c c g c c a c a a g a t a t a g t g g c g g c t g t t c g t c t t c t t g c c g t a g t t c c a c t t g a a g t t c t g g g c g g t g t t

2700 175 180 185 190 IEDGSVQLADHYQQNTPIGD EmGFP IEDGSVQLADHYQQNTPIGD c a t c g a g g a c g g c a g c g t g c a g c t c g c c g a c c a c t a c c a g c a g a a c a c c c c c a t c g g c g a g t a g c t c c t g c c g t c g c a c g t c g a g c g g c t g g t g a t g g t c g t c t t g t g g g g g t a g c c g c t

2760 195 200 205 210 GPVLLPDNHYLSTQSALSKD EmGFP GPVLLPDNHYLSTQSALSKD c g g c c c c g t g c t g c t g c c c g a c a a c c a c t a c c t g a g c a c c c a g t c c g c c c t g a g c a a a g a g c c g g g g c a c g a c g a c g g g c t g t t g g t g a t g g a c t c g t g g g t c a g g c g g g a c t c g t t t c t

2820 215 220 225 230 PNEKRDHMVLLEFVTAAGIT EmGFP C A T G G T C C T G C T G G A G T T C G T G EGFP-C PNEKRDHMVLLEFVTAAGIT c c c c a a c g a g a a g c g c g a t c a c a t g g t c c t g c t g g a g t t c g t g a c c g c c g c c g g g a t c a c g g g g t t g c t c t t c g c g c t a g t g t a c c a g g a c g a c c t c a a g c a c t g g c g g c g g c c c t a g t g

AsiSIBsrGI 2880 235 LGMDELYK* EmGFP SV40 poly(A) signal LGMDELYK* t c t c g g c a t g g a c g a g c t g t a c a a g t a a g c g g c c g c g a t c g c a g a c a t g a t a a g a t a c a t a g a g c c g t a c c t g c t c g a c a t g t t c a t t c g c c g g c g c t a g c g t c t g t a c t a t t c t a t g t a

2940 SV40 poly(A) signal G A A A T SV40pA-R C T A C T C A A A C C T G T T T G G T G EBV-rev t g a t g a g t t t g g a c a a a c c a c a a c t a g a a t g c a g t g a a a a a a a t g c t t t a t t t g t g a a a t a c t a c t c a a a c c t g t t t g g t g t t g a t c t t a c g t c a c t t t t t t t a c g a a a t a a a c a c t t t a

HpaI 3000 SV40 poly(A) signal T T G T G A T G C T A T T G C SV40pA-R t t g t g a t g c t a t t g c t t t a t t t g t a a c c a t t a t a a g c t g c a a t a a a c a a g t t a a c a a c a a a a c a c t a c g a t a a c g a a a t a a a c a t t g g t a a t a t t c g a c g t t a t t t g t t c a a t t g t t g t t

MfeI 3060 c a a t t g c a t t c a t t t t a t g t t t c a g g t t c a g g g g g a g a t g t g g g a g g t t t t t t a a a g c a a g t t a a c g t a a g t a a a a t a c a a a g t c c a a g t c c c c c t c t a c a c c c t c c a a a a a a t t t c g t t

BsaBI* 3120 g t a a a a c c t c t a c a a a t g t g g t a g t c g a a a t t c c c g a t a a g g a t c t t c c t a g a g c a t g g c c a t t t t g g a g a t g t t t a c a c c a t c a g c t t t a a g g g c t a t t c c t a g a a g g a t c t c g t a c c g

3180 AAV2 ITR t a c g t a g a t a a g t a g c a t g g c g g g t t a a t c a t t a a c t a c a a g g a a c c c c t a g t g a t g g a g a t g c a t c t a t t c a t c g t a c c g c c c a a t t a g t a a t t g a t g t t c c t t g g g g a t c a c t a c c t c

3240 AAV2 ITR t t g g c c a c t c c c t c t c t g c g c g c t c g c t c g c t c a c t g a g g c c g g g c g a c c a a a g g t c g c c a a c c g g t g a g g g a g a g a c g c g c g a g c g a g c g a g t g a c t c c g g c c c g c t g g t t t c c a g c g g

3300 AAV2 ITR c g a c g c c c g g g c t t t g c c c g g g c g g c c t c a g t g a g c g a g c g a g c g c g c a g c c t t a a t t a a g c t g c g g g c c c g a a a c g g g c c c g c c g g a g t c a c t c g c t c g c t c g c g c g t c g g a a t t a a t t

3360 M13 fwd T G A C C G G C A G C A A A A T G T M13 Forward G C A A A A T G T T G C A G C A C T G A C C C M13/pUC Forward c c t a a t t c a c t g g c c g t c g t t t t a c a a c g t c g t g a c t g g g a a a a c c c t g g c g t t a c c c a a g g a t t a a g t g a c c g g c a g c a a a a t g t t g c a g c a c t g a c c c t t t t g g g a c c g c a a t g g g t t

3420 c t t a a t c g c c t t g c a g c a c a t c c c c c t t t c g c c a g c t g g c g t a a t a g c g a a g a g g c c c g c g a a t t a g c g g a a c g t c g t g t a g g g g g a a a g c g g t c g a c c g c a t t a t c g c t t c t c c g g g c g

3480 f1 ori a c c g a t c g c c c t t c c c a a c a g t t g c g c a g c c t g a a t g g c g a a t g g g a c g c g c c c t g t a g c t g g c t a g c g g g a a g g g t t g t c a a c g c g t c g g a c t t a c c g c t t a c c c t g c g c g g g a c a t c g

3540 f1 ori g g c g c a t t a a g c g c g g c g g g t g t g g t g g t t a c g c g c a g c g t g a c c g c t a c a c t t g c c a g c c c g c g t a a t t c g c g c c g c c c a c a c c a c c a a t g c g c g t c g c a c t g g c g a t g t g a a c g g t c g

3600 f1 ori G A G G A A A G C G A A A G A A G G G A F1ori-R g c c c t a g c g c c c g c t c c t t t c g c t t t c t t c c c t t c c t t t c t c g c c a c g t t c g c c g g c t t t c g g g a t c g c g g g c g a g g a a a g c g a a a g a a g g g a a g g a a a g a g c g g t g c a a g c g g c c g a a a

3660 f1 ori c c c c g t c a a g c t c t a a a t c g g g g g c t c c c t t t a g g g t t c c g a t t t a g t g c t t t a c g g c a c g g g g c a g t t c g a g a t t t a g c c c c c g a g g g a a a t c c c a a g g c t a a a t c a c g a a a t g c c g t g

DraIII 3720 f1 ori c t c g a c c c c a a a a a a c t t g a t t a g g g t g a t g g t t c a c g t a g t g g g c c a t c g c c c t g a t a g g a g c t g g g g t t t t t t g a a c t a a t c c c a c t a c c a a g t g c a t c a c c c g g t a g c g g g a c t a t c

3780 f1 ori G T G G A C T C T T G T T C C A A F1ori-F a c g g t t t t t c g c c c t t t g a c g t t g g a g t c c a c g t t c t t t a a t a g t g g a c t c t t g t t c c a a t g c c a a a a a g c g g g a a a c t g c a a c c t c a g g t g c a a g a a a t t a t c a c c t g a g a a c a a g g t t

3840 f1 ori A C T G G F1ori-F a c t g g a a c a a c a c t c a a c c c t a t c t c g g t c t a t t c t t t t g a t t t a t a a g g g a t t t t g c c g t g a c c t t g t t g t g a g t t g g g a t a g a g c c a g a t a a g a a a a c t a a a t a t t c c c t a a a a c g g c

3900 f1 ori a t t t c g g c c t a t t g g t t a a a a a a t g a g c t g a t t t a a c a a a a a t t t a a c g c g a a t t t t a a c t a a a g c c g g a t a a c c a a t t t t t t a c t c g a c t a a a t t g t t t t t a a a t t g c g c t t a a a a t t g

3960 f1 ori AmpR promoter a a a a t a t t a a c g c t t a c a a t t t a g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t t a t a a t t g c g a a t g t t a a a t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t

4020 AmpR promoter t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a

4080 1 5 signal sequence MSIQHFRVA AmpR promoter AmpR MSIQHFRVA a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t a t g a g t a t t c a a c a t t t c c g t g t c g c c c t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a t a c t c a t a a g t t g t a a a g g c a c a g c g g g

4140 10 15 20 signal sequence LIPFFAAFCLPVFA 25 HPETLV AmpR LIPFFAAFCLPVFAHPETLV t t a t t c c c t t t t t t g c g g c a t t t t g c c t t c c t g t t t t t g c t c a c c c a g a a a c g c t g g t g a a a t a a g g g a a a a a a c g c c g t a a a a c g g a a g g a c a a a a a c g a g t g g g t c t t t g c g a c c a c t

4200 30 35 40 45 KVKDAEDQLGARVGYIELDL AmpR KVKDAEDQLGARVGYIELDL a a g t a a a a g a t g c t g a a g a t c a g t t g g g t g c a c g a g t g g g t t a c a t c g a a c t g g a t c t c a t t c a t t t t c t a c g a c t t c t a g t c a a c c c a c g t g c t c a c c c a a t g t a g c t t g a c c t a g a g t

XmnI 4260 50 55 60 65 NSGKILESFRPEERFPMMST AmpR NSGKILESFRPEERFPMMST a c a g c g g t a a g a t c c t t g a g a g t t t t c g c c c c g a a g a a c g t t t t c c a a t g a t g a g c a c t t t g t c g c c a t t c t a g g a a c t c t c a a a a g c g g g g c t t c t t g c a a a a g g t t a c t a c t c g t g a a

4320 70 75 80 85 FKVLLCGAVLSRIDAGQEQL AmpR C G A T A C A C C G C G C C A T A A T A Amp-R FKVLLCGAVLSRIDAGQEQL t t a a a g t t c t g c t a t g t g g c g c g g t a t t a t c c c g t a t t g a c g c c g g g c a a g a g c a a c t c g a a t t t c a a g a c g a t a c a c c g c g c c a t a a t a g g g c a t a a c t g c g g c c c g t t c t c g t t g a g c

ScaI 4380 90 95 100 105 GRRIHYSQNDLVEYSPVTEK AmpR GRRIHYSQNDLVEYSPVTEK g t c g c c g c a t a c a c t a t t c t c a g a a t g a c t t g g t t g a g t a c t c a c c a g t c a c a g a a a a g c c a g c g g c g t a t g t g a t a a g a g t c t t a c t g a a c c a a c t c a t g a g t g g t c a g t g t c t t t t c g

4440 110 115 120 125 HLTDGMTVRELCSAAITMSD AmpR HLTDGMTVRELCSAAITMSD a t c t t a c g g a t g g c a t g a c a g t a a g a g a a t t a t g c a g t g c t g c c a t a a c c a t g a g t g a t a t a g a a t g c c t a c c g t a c t g t c a t t c t c t t a a t a c g t c a c g a c g g t a t t g g t a c t c a c t a t

4500 130 135 140 145 NTAANLLLTTIGGPKELTAF AmpR NTAANLLLTTIGGPKELTAF a c a c t g c g g c c a a c t t a c t t c t g a c a a c g a t c g g a g g a c c g a a g g a g c t a a c c g c t t t t t t g t g a c g c c g g t t g a a t g a a g a c t g t t g c t a g c c t c c t g g c t t c c t c g a t t g g c g a a a a a

4560 150 155 160 165 LHNMGDHVTRLDRWEPELNE AmpR LHNMGDHVTRLDRWEPELNE t g c a c a a c a t g g g g g a t c a t g t a a c t c g c c t t g a t c g t t g g g a a c c g g a g c t g a a t g a a g a c g t g t t g t a c c c c c t a g t a c a t t g a g c g g a a c t a g c a a c c c t t g g c c t c g a c t t a c t t c

4620 170 175 180 185 AIPNDERDTTMPVAMATTLR AmpR AIPNDERDTTMPVAMATTLR c c a t a c c a a a c g a c g a g c g t g a c a c c a c g a t g c c t g t a g c a a t g g c a a c a a c g t t g c g c a g g t a t g g t t t g c t g c t c g c a c t g t g g t g c t a c g g a c a t c g t t a c c g t t g t t g c a a c g c g t

4680 190 195 200 205 KLLTGELLTLASRQQLIDWM AmpR KLLTGELLTLASRQQLIDWM a a c t a t t a a c t g g c g a a c t a c t t a c t c t a g c t t c c c g g c a a c a a t t a a t a g a c t g g a t g g t t g a t a a t t g a c c g c t t g a t g a a t g a g a t c g a a g g g c c g t t g t t a a t t a t c t g a c c t a c c

4740 210 215 220 225 EADKVAGPLLRSALPAGWFI AmpR EADKVAGPLLRSALPAGWFI a g g c g g a t a a a g t t g c a g g a c c a c t t c t g c g c t c g g c c c t t c c g g c t g g c t g g t t t a t t g t c c g c c t a t t t c a a c g t c c t g g t g a a g a c g c g a g c c g g g a a g g c c g a c c g a c c a a a t a a c

4800 230 235 240 245 ADKSGAGERGSRGIIAALGP AmpR ADKSGAGERGSRGIIAALGP c t g a t a a a t c t g g a g c c g g t g a g c g t g g g t c t c g c g g t a t c a t t g c a g c a c t g g g g c c a g g a c t a t t t a g a c c t c g g c c a c t c g c a c c c a g a g c g c c a t a g t a a c g t c g t g a c c c c g g t c

4860 250 255 260 265 DGKPSRIVVIYTTGSQATMD AmpR DGKPSRIVVIYTTGSQATMD a t g g t a a g c c c t c c c g t a t c g t a g t t a t c t a c a c g a c g g g g a g t c a g g c a a c t a t g g a t g t a c c a t t c g g g a g g g c a t a g c a t c a a t a g a t g t g c t g c c c c t c a g t c c g t t g a t a c c t a c

4920 270 275 280 285 ERNRQIAEIGASLIKHW* AmpR ERNRQIAEIGASLIKHW* a a c g a a a t a g a c a g a t c g c t g a g a t a g g t g c c t c a c t g a t t a a g c a t t g g t a a c t g t c a g t t g c t t t a t c t g t c t a g c g a c t c t a t c c a c g g a g t g a c t a a t t c g t a a c c a t t g a c a g t c

4980 a c c a a g t t t a c t c a t a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t g g t t c a a a t g a g t a t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t

5040 t c t a g g t g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t a g a t c c a c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a

5100 ori t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g

5160 ori t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g

5220 ori c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g

5280 ori c a a a t a c t g t t c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c g t t t a t g a c a a g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g

5340 ori c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a

5400 ori c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a

BseYIPspFI 5460 ori g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a

5520 ori a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t t g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a

5580 ori G G G A A A C G pBR322ori-F a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c

5640 ori C C T G G T A T C T T T pBR322ori-F c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a

5700 ori g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a

NspIPciI 5760 t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c

5820 t g g a t a a c c g t a t t a c c g c c t t t g a g t g a g c t g a t a c c g c t c g c c g c a g c c g a a c g a c c g a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c g a c t a t g g c g a g c g g c g t c g g c t t g c t g g c

BspQISapI 5880 a g c g c a g c g a g t c a g t g a g c g a g g a a g c g g a a g a g c g c c c a a t a c g c a a a c c g c c t c t c c t c g c g t c g c t c a g t c a c t c g c t c c t t c g c c t t c t c g c g g g t t a t g c g t t t g g c g g a g a g g

5940 c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g g c a c g a c a g g t t t c c c g a c t g g a a a g c g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c c g t g c t g t c c a a a g g g c t g a c c t t t c g c c

6000 -35 CAP binding site lac promoter g c a g t g a g c g c a a c g c a a t t a a t g t g a g t t a g c t c a c t c a t t a g g c a c c c c a g g c t t t a c c g t c a c t c g c g t t g c g t t a a t t a c a c t c a a t c g a g t g a g t a a t c c g t g g g g t c c g a a a t g

6060 -10 lac promoter lac operator M13 rev A G C G G A T A A C A A T T T C A C A C A G M13/pUC Reverse C A G M13 Reverse a c t t t a t g c t t c c g g c t c g t a t g t t g t g t g g a a t t g t g a g c g g a t a a c a a t t t c a c a c a g t g a a a t a c g a a g g c c g a g c a t a c a a c a c a c c t t a a c a c t c g c c t a t t g t t a a a g t g t g t c

6100 M13 rev G M13/pUC Reverse G A A A C A G C T A T G A C M13 Reverse g a a a c a g c t a t g a c c a t g a t t a c g c c a g a t t t a a t t a a g g c t t t g t c g a t a c t g g t a c t a a t g c g g t c t a a a t t a a t t c c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.