Addgene pLenti6-BMPR1A(Q233D)-V5 - Sequence Analyzer Skip to main content
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Sequence Analyzer: pLenti6-BMPR1A(Q233D)-V5


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6000 4000 2000 End (7233) L4440 (7205 .. 7222) DrdI (7005) pBR322ori-F (6952 .. 6971) AhdI (6219) BglI (6101) NmeAIII (6072) Amp-R (5650 .. 5669) MluI (5347) PmeI (5341) EBV-rev (5292 .. 5311) SV40pA-R (5238 .. 5257) HpaI (5200) SV40 NLS small t intron BmtI (4481) NheI (4477) SalI (4339) BfuAI - BspMI (4326) BstXI (4199) ccdB-fwd (4183 .. 4202) BmgBI (4116) SrfI (4082) PasI (3444) CAT-R (3065 .. 3084) lac UV5 promoter AgeI (2758) SacI - BanII (2755) Eco53kI (2753) AleI (2739) KpnI (2734) Acc65I (2730) AvrII (2723) XhoI - PaeR7I (2695) BstBI (2689) HindIII (2681) StuI (2669) EcoRV (2662) BlpI (2520) AfeI (2518) PpuMI - EcoO109I (2338) NdeI (2201) SpeI (2037) AflII (2013) BclI * (1899) ClaI - BspDI (1787) BstAPI (1772) NsiI (1502) PluTI (1488) SfoI (1486) NarI (1485) KasI (1484) NruI (603) AsiSI (479) BglII (216) Start (0) Ac5 promoter attR1 CmR ccdB attR2 SV40 poly(A) signal AmpR ori pLenti6-BMPR1A(Q233D)-V5 7233 bp

Sequence View

Start (0) 5ʹ 3ʹ 60 g c g c c c a a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g g c a a c t c g c g g g t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c c g t t g a

120 c g t g a a a g g t a g g c g g a t c a g c g g c c g c a g c a t g c a a t t c t a t a t t c t a a a a a c a c a a a t g c a c t t t c c a t c c g c c t a g t c g c c g g c g t c g t a c g t t a a g a t a t a a g a t t t t t g t g t t t a

180 Ac5 promoter g a t a c t t c t a a a a a a a a a t c a t g a a t g g c a t c a a c t c t g a a t c a a a t c t t t g c a g a t g c a c t a t g a a g a t t t t t t t t t a g t a c t t a c c g t a g t t g a g a c t t a g t t t a g a a a c g t c t a c g t

BglII 240 Ac5 promoter c c t a c t t c t c a t t t c c a c t g t c a c a t c a t t t t t c c a g a t c t c g c t g c c t g t t a t g t g g c c g g a t g a a g a g t a a a g g t g a c a g t g t a g t a a a a a g g t c t a g a g c g a c g g a c a a t a c a c c g g

300 Ac5 promoter c a c a a a c c a a g a c a c g t t t t a t g g c c a t t a a a g c t g g c t g a t c g t c g c c a a a c a c c a a a t g t g t t t g g t t c t g t g c a a a a t a c c g g t a a t t t c g a c c g a c t a g c a g c g g t t t g t g g t t t a

360 Ac5 promoter a c a t a a t g a a t a t g t a c a c a t t c g a g a a a g a a g c g a t c a a a g a a g c g t c t t c g g g c g g a g t g t a t t a c t t a t a c a t g t g t a a g c t c t t t c t t c g c t a g t t t c t t c g c a g a a g c c c g c c t c

420 Ac5 promoter t a g g a g a a t g c g g a g g a g a a g g a g a a c g a g c t g a t c t a g t a t c t c t c c a c a a t c c a a t g c a t c c t c t t a c g c c t c c t c t t c c t c t t g c t c g a c t a g a t c a t a g a g a g g t g t t a g g t t a c g

AsiSI 480 Ac5 promoter c a a c t g a c c a a c t g g c c a t a t t c g g a g c a a t t t g a a g c c a a t t t c c a t c g c c t g g c g a t c g t t g a c t g g t t g a c c g g t a t a a g c c t c g t t a a a c t t c g g t t a a a g g t a g c g g a c c g c t a g

540 Ac5 promoter g c t c c a t t c t t g g c t a t a t g t t t t t c a c c g t t a c c c g g g g c c a t t t t c a a a g a c t c g t c g c g a g g t a a g a a c c g a t a t a c a a a a a g t g g c a a t g g g c c c c g g t a a a a g t t t c t g a g c a g c

600 Ac5 promoter g c a a g a t a a g a t t g t g t c a c t c g c t g t c t c t c t t c a t t t g t c g a a g a a t g c t g a g g a a t t c g t t c t a t t c t a a c a c a g t g a g c g a c a g a g a g a a g t a a a c a g c t t c t t a c g a c t c c t t a a

NruI 660 Ac5 promoter t c g c g a t g a c g t c g g c g a g t a t t t t g a a g a a t g a g a a t a a t t t g t a t t t a t a c g a a a a t c a g c g c t a c t g c a g c c g c t c a t a a a a c t t c t t a c t c t t a t t a a a c a t a a a t a t g c t t t t a g

720 Ac5 promoter a g t t a g t g g a a t t t t c t a c a a a a a c a t g t t a t c t a t a g a t a a t t t t g t t g c a a a a t a t g t t c a a t c a c c t t a a a a g a t g t t t t t g t a c a a t a g a t a t c t a t t a a a a c a a c g t t t t a t a c a

780 Ac5 promoter t g a c t a t g a c a a a g a t t g t a t g t a t a t a c c t t t a a t g t a t t c t c a t t t t c t t a t g t a t t t a c t g a t a c t g t t t c t a a c a t a c a t a t a t g g a a a t t a c a t a a g a g t a a a a g a a t a c a t a a a

840 Ac5 promoter a t a a t g g c a a t g a t g a t a c t g a t g a t a t t t t a a g a t g a t g c c a g a c c a a a a g g c t t g a a t t a t t a c c g t t a c t a c t a t g a c t a c t a t a a a a t t c t a c t a c g g t c t g g t t t t c c g a a c t t a

900 Ac5 promoter t t c t g c g t c t t t t g c c g a a c g c a g t g c a t g t g c a a t t g t t g t t t t t t g g a a t a t t c a a t t a a g a c g c a g a a a a c g g c t t g c g t c a c g t a c a c g t t a a c a a c a a a a a a c c t t a t a a g t t a a

960 Ac5 promoter t t c g g a c t g t c c g c t t t g a t t t c a g t t t c t t g g c t t a t t c a a a a a g c a a a g t a a a g c c a a a a g c c t g a c a g g c g a a a c t a a a g t c a a a g a a c c g a a t a a g t t t t t c g t t t c a t t t c g g t t

1020 Ac5 promoter MRQNGSGRKGVRG a a a a g c g a g a t g g c a a t a c c a a a t g c g g c a a a a c g g t a g t g g a a g g a a a g g g g t g c g g g g t t t t c g c t c t a c c g t t a t g g t t t a c g c c g t t t t g c c a t c a c c t t c c t t t c c c c a c g c c c c

1080 Ac5 promoter SGRKGGAGRGGVCGWARRHR c a g c g g a a g g a a g g g t g g g g c g g g g c g t g g c g g g g t c t g t g g c t g g g c g c g a c g t c a c c g g t c g c c t t c c t t c c c a c c c c g c c c c g c a c c g c c c c a g a c a c c g a c c c g c g c t g c a g t g g c

1140 Ac5 promoter RWSHSFDHVCVCVLFVSRHS a c g t t g g a g c c a c t c c t t t g a c c a t g t g t g c g t g t g t g t a t t a t t c g t g t c t c g c c a c t c t g c a a c c t c g g t g a g g a a a c t g g t a c a c a c g c a c a c a c a t a a t a a g c a c a g a g c g g t g a g

1200 Ac5 promoter PVVFFFLCCALSSAISLTHA g c c g g t t g t t t t t t t c t t t t t a t g c t g c g c t c t c t c t a g c g c c a t c t c g c t t a c g c a t g c c g g c c a a c a a a a a a a g a a a a a t a c g a c g c g a g a g a g a t c g c g g t a g a g c g a a t g c g t a c g

1260 Ac5 promoter QRTACCRFLLCVILARNRQL t c a a c g c a c c g c a t g t t g c c g t t t c c t t t t a t g c g t c a t t t t g g c t c g a a a t a g g c a a t t a g t t g c g t g g c g t a c a a c g g c a a a g g a a a a t a c g c a g t a a a a c c g a g c t t t a t c c g t t a a

1320 Ac5 promoter FKQRLVNENAKINKSTIWLL a t t t a a a c a a a g a t t a g t c a a c g a a a a c g c t a a a a t a a a t a a g t c t a c a a t a t g g t t a c t t a a a t t t g t t t c t a a t c a g t t g c t t t t g c g a t t t t a t t t a t t c a g a t g t t a t a c c a a t g a

1380 Ac5 promoter IAMCVQPTIATKATTQVAFP t a t t g c c a t g t g t g t g c a g c c a a c g a t a g c a a c a a a a g c a a c a a c a c a g g t g g c t t t c c c a t a a c g g t a c a c a c a c g t c g g t t g c t a t c g t t g t t t t c g t t g t t g t g t c c a c c g a a a g g g

1440 Ac5 promoter LSLFVCKPRASKTARPANAI t c t t t c a c t t t t t g t t t g c a a g c c g c g t g c g a g c a a g a c g g c a c g a c c g g c a a a c g c a a t a g a a a g t g a a a a a c a a a c g t t c g g c g c a c g c t c g t t c t g c c g t g c t g g c c g t t t g c g t t a

KasINarISfoIPluTI 1500 Ac5 promoter TLTKSRRSFGEKHQGA* t a c g c t g a c a a a g a g c a g a c g a a g t t t t g g c g a a a a a c a t c a a g g c g c c t g a t a c g a a t g a t g c g a c t g t t t c t c g t c t g c t t c a a a a c c g c t t t t t g t a g t t c c g c g g a c t a t g c t t a c

NsiI 1560 Ac5 promoter c a t t t g c a a t a a c a a t t g c g a t a t t t a a t a t t g t t t a t g a a g c t g t t t g a c t t c a a a a c a g t a a a c g t t a t t g t t a a c g c t a t a a a t t a t a a c a a a t a c t t c g a c a a a c t g a a g t t t t g t

1620 Ac5 promoter c a c a a a a a a a a a a a t a a a a c a a a t t a t t t g a a a g a g a a t t a g g a a t c g g a c g c t t a t c g t g t g t t t t t t t t t t t a t t t t g t t t a a t a a a c t t t c t c t t a a t c c t t a g c c t g c g a a t a g c a

1680 Ac5 promoter t a g g g t a a c a a c a a g a a a t g c t t a c t g a g t c a c a g c c t c t g g a a a a c t g c c g c a a g c c a g a t c c c a t t g t t g t t c t t t a c g a a t g a c t c a g t g t c g g a g a c c t t t t g a c g g c g t t c g g t c

1740 Ac5 promoter a g a g a g a g a g a a a a a g a g g g a g a g c a g c t t a g a c c g c a t g t g c t t g t g t g t g a g g c g t c t t c t c t c t c t c t t t t t c t c c c t c t c g t c g a a t c t g g c g t a c a c g a a c a c a c a c t c c g c a g a

BspDIClaIBstAPI 1800 Ac5 promoter c t c t c t t c g t c t c t g t t g c g c a a a c g c a t a g a c t g c a c t g a g a a a a t c g a t t a c c t a t t t g a g a g a a g c a g a g a c a a c g c g t t t g c g t a t c t g a c g t g a c t c t t t t a g c t a a t g g a t a a a

1860 Ac5 promoter t t t a t g a a t g a a t a t t t g c a c t a t t a c t a t t c a a a a c t a t t a a g a t a g c a a t c a c a t t c a a a a t a c t t a c t t a t a a a c g t g a t a a t g a t a a g t t t t g a t a a t t c t a t c g t t a g t g t a a g t

BclI* 1920 Ac5 promoter a t a g c c a a a t a c t a t a c c a c c t g a g c g a t g c a a c g a a a t g a t c a a t t t g a g c a a a a a t g c t a t c g g t t t a t g a t a t g g t g g a c t c g c t a c g t t g c t t t a c t a g t t a a a c t c g t t t t t a c g

1980 Ac5 promoter t g c a t a t t t a g g a c g g c a t c a t t a t a g a a a t g c t t c t t g c t g t g t a c t t t t c t c t c g t c t a c g t a t a a a t c c t g c c g t a g t a a t a t c t t t a c g a a g a a c g a c a c a t g a a a a g a g a g c a g a

SpeIAflII 2040 Ac5 promoter *FRSLNPTNEVVL g g c a g c t g t t t c g c c g t t a t t g t t a a a a c c g g c t t a a g t t a g g t g t g t t t t c t a c g a c t a c c g t c g a c a a a g c g g c a a t a a c a a t t t t g g c c g a a t t c a a t c c a c a c a a a a g a t g c t g a t

2100 Ac5 promoter SHGVLLHTNCLIDRSYGIQL g t g a a t g c c c t a c t a g a a g a t g t g t g t t g c a c a a a a t g t c c c t g g a a t a a c c a a t t t g a a c a c t t a c g g g a t g a t c t t c t a c a c a c a a c g t g t t t t a c a g g g a c c t t a t t g g t t a a a c t t

2160 Ac5 promoter ASLLLRLSIHINNLQLTKID g t g c a g a t a g c a g t a a a c g t a a g c t a a t a t g a a t a t t a t t t a a c t g t a a t g t t t t a a t a t c a c g t c t a t c g t c a t t t g c a t t c g a t t a t a c t t a t a a t a a a t t g a c a t t a c a a a a t t a t a

NdeI 2220 Ac5 promoter SSMVLLGVIFVHVYTHKPMC c g c t g g a c a t t a c t a a t a a a c c c a c t a t a a a c a c a t g t a c a t a t g t a t g t t t t g g c a t a c g c g a c c t g t a a t g a t t a t t t g g g t g a t a t t t g t g t a c a t g t a t a c a t a c a a a a c c g t a t g

2280 Ac5 promoter HTTPSFIHLLVTVMVAYLYG a a t g a g t a g t t g g g g a a a a a a t g t g t a a a a g c a c c g t g a c c a t c a c a g c a t a a a g a t a a c t t a c t c a t c a a c c c c t t t t t t a c a c a t t t t c g t g g c a c t g g t a g t g t c g t a t t t c t a t t g

EcoO109IPpuMI 2340 Ac5 promoter ASTDFILLGWISDCAAVSLV c a g c t g a a g t a t c g a a t a t g a g t a a c c c c c a a a t t g a a t c a c a t g c c g c a a c t g a t a g g a g t c g a c t t c a t a g c t t a t a c t c a t t g g g g g t t t a a c t t a g t g t a c g g c g t t g a c t a t c c t

2400 Ac5 promoter WPLVSKMAIYLVCVLVFVWN c c c a t g g a a g t a c a c t c t t c a t g g c g a t a t a c a a g a c a c a c a c a a g c a c g a a c a c c c a g t g g g t a c c t t c a t g t g a g a a g t a c c g c t a t a t g t t c t g t g t g t g t t c g t g c t t g t g g g t c a

2460 Ac5 promoter RLFNETFSFGIPSCNMGMYP t g c g g a g g a a a t t c t c c g t a a a t g a a a a c c c a a t c g g c g a a c a a t t c a t a c c c a t a t a t g a c g c c t c c t t t a a g a g g c a t t t a c t t t t g g g t t a g c c g c t t g t t a a g t a t g g g t a t a t a c

AfeI 2520 Ac5 promoter LLKSRSKLASLM g t a a a a g t t t t g a a c g c g a c t t g a g a g c g g a g a g c a t t g c g g c t g a t a a g g t t t t a g c g c c a t t t t c a a a a c t t g c g c t g a a c t c t c g c c t c t c g t a a c g c c g a c t a t t c c a a a a t c g c g

BlpI 2580 Ac5 promoter t a a g c g g g c t t t a t a a a a c g g g c t g c g g g a c c a g t t t t c a t a t c a c t a c c g t t t g a g t t c a t t c g c c c g a a a t a t t t t g c c c g a c g c c c t g g t c a a a a g t a t a g t g a t g g c a a a c t c a a g

2640 Ac5 promoter t t g t g c t g t g t g g a t a c t c c t c c c g a c a c a a a g c c g c t c c a t c a g c c a g c a g t c g t c t a a a a c a c g a c a c a c c t a t g a g g a g g g c t g t g t t t c g g c g a g g t a g t c g g t c g t c a g c a g a t t

PaeR7IXhoIBstBIHindIIIStuIEcoRV 2700 Ac5 promoter t c c a g a g a c c c c g g a t c c a g a t a t c g a g g c c t g t c t a g a g a a g c t t g t t c g a a t c t c g a g a g g t c t c t g g g g c c t a g g t c t a t a g c t c c g g a c a g a t c t c t t c g a a c a a g c t t a g a g c t c

Eco53kIBanIISacIAgeIAleIAvrIIAcc65IKpnI 2760 t g c g c g c t t c c g g a g g t a t a c a c c t a g g c g g t a c c a c t g c a g t g a a t t c g g a g c t c t a c c a c g c g c g a a g g c c t c c a t a t g t g g a t c c g c c a t g g t g a c g t c a c t t a a g c c t c g a g a t g g

2820 attR1 g g t a t a c a a g t t t g t a c a a a a a a g c t g a a c g a g a a a c g t a a a a t g a t a t a a a t a t c a a t a c c a t a t g t t c a a a c a t g t t t t t t c g a c t t g c t c t t t g c a t t t t a c t a t a t t t a t a g t t a t

2880 attR1 t a t t a a a t t a g a t t t t g c a t a a a a a a c a g a c t a c a t a a t a c t g t a a a a c a c a a c a t a t c c a t a a t t t a a t c t a a a a c g t a t t t t t t g t c t g a t g t a t t a t g a c a t t t t g t g t t g t a t a g g

2940 -35 attR1 lac UV5 promoter a g t c a c t a t g g c g g c c g c a t t a g g c a c c c c a g g c t t t a c a c t t t a t g c t t c c g g c t c g t a t c a g t g a t a c c g c c g g c g t a a t c c g t g g g g t c c g a a a t g t g a a a t a c g a a g g c c g a g c a t

3000 -10 1 M lac UV5 promoter CmR M t a a t g t g t g g a t t t t g a g t t a g g a t c c g t c g a g a t t t t c a g g a g c t a a g g a a g c t a a a a t a t t a c a c a c c t a a a a c t c a a t c c t a g g c a g c t c t a a a a g t c c t c g a t t c c t t c g a t t t t a

3060 5 10 15 20 EKKITGYTTVDISQWHRKEH CmR EKKITGYTTVDISQWHRKEH g g a g a a a a a a a t c a c t g g a t a t a c c a c c g t t g a t a t a t c c c a a t g g c a t c g t a a a g a a c a c c t c t t t t t t t a g t g a c c t a t a t g g t g g c a a c t a t a t a g g g t t a c c g t a g c a t t t c t t g t

3120 25 30 35 40 FEAFQSVAQCTYNQTVQLDI CmR C T C C G T A A A G T C A G T C A A C G CAT-R FEAFQSVAQCTYNQTVQLDI t t t t g a g g c a t t t c a g t c a g t t g c t c a a t g t a c c t a t a a c c a g a c c g t t c a g c t g g a t a t a a a a c t c c g t a a a g t c a g t c a a c g a g t t a c a t g g a t a t t g g t c t g g c a a g t c g a c c t a t a

3180 45 50 55 60 TAFLKTVKKNKHKFYPAFIH CmR TAFLKTVKKNKHKFYPAFIH t a c g g c c t t t t t a a a g a c c g t a a a g a a a a a t a a g c a c a a g t t t t a t c c g g c c t t t a t t c a a t g c c g g a a a a a t t t c t g g c a t t t c t t t t t a t t c g t g t t c a a a a t a g g c c g g a a a t a a g t

3240 65 70 75 80 ILARLMNAHPEFRMAMKDGE CmR ILARLMNAHPEFRMAMKDGE c a t t c t t g c c c g c c t g a t g a a t g c t c a t c c g g a a t t c c g t a t g g c a a t g a a a g a c g g t g a g t a a g a a c g g g c g g a c t a c t t a c g a g t a g g c c t t a a g g c a t a c c g t t a c t t t c t g c c a c t

3300 85 90 95 100 LVIWDSVHPCYTVFHEQTET CmR LVIWDSVHPCYTVFHEQTET g c t g g t g a t a t g g g a t a g t g t t c a c c c t t g t t a c a c c g t t t t c c a t g a g c a a a c t g a a a c c g a c c a c t a t a c c c t a t c a c a a g t g g g a a c a a t g t g g c a a a a g g t a c t c g t t t g a c t t t g

3360 105 110 115 120 FSSLWSEYHDDFRQFLHIYS CmR FSSLWSEYHDDFRQFLHIYS g t t t t c a t c g c t c t g g a g t g a a t a c c a c g a c g a t t t c c g g c a g t t t c t a c a c a t a t a t t c c a a a a g t a g c g a g a c c t c a c t t a t g g t g c t g c t a a a g g c c g t c a a a g a t g t g t a t a t a a g

3420 125 130 135 140 QDVACYGENLAYFPKGFIEN CmR QDVACYGENLAYFPKGFIEN g c a a g a t g t g g c g t g t t a c g g t g a a a a c c t g g c c t a t t t c c c t a a a g g g t t t a t t g a g a a c g t t c t a c a c c g c a c a a t g c c a c t t t t g g a c c g g a t a a a g g g a t t t c c c a a a t a a c t c t t

PasI 3480 145 150 155 160 MFFVSANPWVSFTSFDLNVA CmR MFFVSANPWVSFTSFDLNVA t a t g t t t t t c g t c t c a g c c a a t c c c t g g g t g a g t t t c a c c a g t t t t g a t t t a a a c g t g g c a t a c a a a a a g c a g a g t c g g t t a g g g a c c c a c t c a a a g t g g t c a a a a c t a a a t t t g c a c c g

3540 165 170 175 180 NMDNFFAPVFTMGKYYTQGD CmR NMDNFFAPVFTMGKYYTQGD c a a t a t g g a c a a c t t c t t c g c c c c c g t t t t c a c c a t g g g c a a a t a t t a t a c g c a a g g c g a g t t a t a c c t g t t g a a g a a g c g g g g g c a a a a g t g g t a c c c g t t t a t a a t a t g c g t t c c g c t

3600 185 190 195 200 KVLMPLAIQVHHAVCDGFHV CmR KVLMPLAIQVHHAVCDGFHV c a a g g t g c t g a t g c c g c t g g c g a t t c a g g t t c a t c a t g c c g t t t g t g a t g g c t t c c a t g t g t t c c a c g a c t a c g g c g a c c g c t a a g t c c a a g t a g t a c g g c a a a c a c t a c c g a a g g t a c a

3660 205 210 215 220 GRMLNELQQYCDEWQAGRNL CmR GRMLNELQQYCDEWQAGRNL c g g c a g a a t g c t t a a t g a a t t a c a a c a g t a c t g c g a t g a g t g g c a g g c g g g g c g t a a t c t g c c g t c t t a c g a a t t a c t t a a t g t t g t c a t g a c g c t a c t c a c c g t c c g c c c c g c a t t a g a

3720 225 EDPAY* CmR EDPAY* a g a g g a t c c g g c t t a c t a a a a g c c a g a t a a c a g t a t g c g t a t t t g c g c g c t g a t t t t t g c t c t c c t a g g c c g a a t g a t t t t c g g t c t a t t g t c a t a c g c a t a a a c g c g c g a c t a a a a a c g

3780 g g t a t a a g a a t a t a t a c t g a t a t g t a t a c c c g a a g t a t g t c a a a a a g a g g t a t g c t a t g a c c a t a t t c t t a t a t a t g a c t a t a c a t a t g g g c t t c a t a c a g t t t t t c t c c a t a c g a t a c t

3840 a g c a g c g t a t t a c a g t g a c a g t t g a c a g c g a c a g c t a t c a g t t g c t c a a g g c a t a t a t g a t c g t c g c a t a a t g t c a c t g t c a a c t g t c g c t g t c g a t a g t c a a c g a g t t c c g t a t a t a c t

3900 t g t c a a t a t c t c c g g t c t g g t a a g c a c a a c c a t g c a g a a t g a a g c c c g t c g t c t g c g t g c a c a g t t a t a g a g g c c a g a c c a t t c g t g t t g g t a c g t c t t a c t t c g g g c a g c a g a c g c a c g

3960 c g a a c g c t g g a a a g c g g a a a a t c a g g a a g g g a t g g c t g a g g t c g c c c g g t t t a t t g a a a t g c t t g c g a c c t t t c g c c t t t t a g t c c t t c c c t a c c g a c t c c a g c g g g c c a a a t a a c t t t a

4020 1 5 MQFKVYT ccdB g a a c g g c t c t t t t g c t g a c g a g a a c a g g g g c t g g t g a a a t g c a g t t t a a g g t t t a c a c c t c t t g c c g a g a a a a c g a c t g c t c t t g t c c c c g a c c a c t t t a c g t c a a a t t c c a a a t g t g g a

4080 10 15 20 25 YKRESRYRLFVDVQSDIIDT ccdB a t a a a a g a g a g a g c c g t t a t c g t c t g t t t g t g g a t g t a c a g a g t g a t a t t a t t g a c a c g c t a t t t t c t c t c t c g g c a a t a g c a g a c a a a c a c c t a c a t g t c t c a c t a t a a t a a c t g t g c g

BmgBISrfI 4140 30 35 40 45 PGRRMVIPLASARLLSDKVP ccdB c c g g g c g a c g g a t g g t g a t c c c c c t g g c c a g t g c a c g t c t g c t g t c a g a t a a a g t c c c c c g g c c c g c t g c c t a c c a c t a g g g g g a c c g g t c a c g t g c a g a c g a c a g t c t a t t t c a g g g g g

BstXI 4200 50 55 60 65 RELYPVVHIGDESWRMMTTD ccdB G C A T G A T G A C C A C C G A T A ccdB-fwd g t g a a c t t t a c c c g g t g g t g c a t a t c g g g g a t g a a a g c t g g c g c a t g a t g a c c a c c g a t a c a c t t g a a a t g g g c c a c c a c g t a t a g c c c c t a c t t t c g a c c g c g t a c t a c t g g t g g c t a t

4260 70 75 80 85 MASVPVSVIGEEVADLSHRE ccdB T G ccdB-fwd t g g c c a g t g t g c c g g t c t c c g t t a t c g g g g a a g a a g t g g c t g a t c t c a g c c a c c g c g a a a a c c g g t c a c a c g g c c a g a g g c a a t a g c c c c t t c t t c a c c g a c t a g a g t c g g t g g c g c t t t

4320 90 95 100 NDIKNAINLMFWGI* ccdB a t g a c a t c a a a a a c g c c a t t a a c c t g a t g t t c t g g g g a a t a t a a a t g t c a g g c t c c c t t a t a c t g t a g t t t t t g c g g t a a t t g g a c t a c a a g a c c c c t t a t a t t t a c a g t c c g a g g g a a t

SalIBspMIBfuAI 4380 attR2 t a c a c a g c c a g t c t g c a g g t c g a c c a t a g t g a c t g g a t a t g t t g t g t t t t a c a g t a t t a t a t g t g t c g g t c a g a c g t c c a g c t g g t a t c a c t g a c c t a t a c a a c a c a a a a t g t c a t a a t a

4440 attR2 g t a g t c t g t t t t t t a t g c a a a a t c t a a t t t a a t a t a t t g a t a t t t a t a t c a t t t t a c g t t c a t c a g a c a a a a a a t a c g t t t t a g a t t a a a t t a t a t a a c t a t a a a t a t a g t a a a a t g c a a

BmtINheI 4500 attR2 t c t c g t t c a g c t t t c t t g t a c a a a g t g g t g a c g t a a g c t a g c a g g a t c t t t g t g a a g g a a a g a g c a a g t c g a a a g a a c a t g t t t c a c c a c t g c a t t c g a t c g t c c t a g a a a c a c t t c c t t

4560 c c t t a c t t c t g t g g t g t g a c a t a a t t g g a c a a a c t a c c t a c a g a g a t t t a a a g c t c t a a g g g a a t g a a g a c a c c a c a c t g t a t t a a c c t g t t t g a t g g a t g t c t c t a a a t t t c g a g a t t c

4620 small t intron g t a a a t a t a a a a t t t t t a a g t g t a t a a t g t g t t a a a c t a c t g a t t c t a a t t g t t t g t g t a c a t t t a t a t t t t a a a a a t t c a c a t a t t a c a c a a t t t g a t g a c t a a g a t t a a c a a a c a c a t

4680 small t intron t t t t a g a t t c c a a c c t a t g g a a c t g a t g a a t g g g a g c a g t g g t g g a a t g c c t t t a a t g a g a a a a t c t a a g g t t g g a t a c c t t g a c t a c t t a c c c t c g t c a c c a c c t t a c g g a a a t t a c t c

4740 MPSSDDEATADS g a a a a c c t g t t t t g c t c a g a a g a a a t g c c a t c t a g t g a t g a t g a g g c t a c t g c t g a c t c t c t t t t g g a c a a a a c g a g t c t t c t t t a c g g t a g a t c a c t a c t a c t c c g a t g a c g a c t g a g a

4800 130 PKKKRKV (in frame with SV40 NLS) EDPKDFPS SV40 NLS QHSTPPKKKRKVEDPKDFPS c a a c a t t c t a c t c c t c c a a a a a a g a a g a g a a a g g t a g a a g a c c c c a a g g a c t t t c c t t c a g t t g t a a g a t g a g g a g g t t t t t t c t t c t c t t t c c a t c t t c t g g g g t t c c t g a a a g g a a g t

4860 (in frame with SV40 NLS) ELLSFLSHAVFSNRTLACFA ELLSFLSHAVFSNRTLACFA g a a t t g c t a a g t t t t t t g a g t c a t g c t g t g t t t a g t a a t a g a a c t c t t g c t t g c t t t g c t c t t a a c g a t t c a a a a a a c t c a g t a c g a c a c a a a t c a t t a t c t t g a g a a c g a a c g a a a c g a

4920 (in frame with SV40 NLS) IYTTKEKAALLYKKIMEKYS IYTTKEKAALLYKKIMEKYS a t t t a c a c c a c a a a g g a a a a a g c t g c a c t g c t a t a c a a g a a a a t t a t g g a a a a a t a t t c t t a a a t g t g g t g t t t c c t t t t t c g a c g t g a c g a t a t g t t c t t t t a a t a c c t t t t t a t a a g a

4980 (in frame with SV40 NLS) VTFISRHNSYNHNILFFLTP VTFISRHNSYNHNILFFLTP g t a a c c t t t a t a a g t a g g c a t a a c a g t t a t a a t c a t a a c a t a c t g t t t t t t c t t a c t c c a c a t t g g a a a t a t t c a t c c g t a t t g t c a a t a t t a g t a t t g t a t g a c a a a a a a g a a t g a g g t

5040 (in frame with SV40 NLS) HRHRVSAINNYAQKLCTFSF HRHRVSAINNYAQKLCTFSF c a c a g g c a t a g a g t g t c t g c t a t t a a t a a c t a t g c t c a a a a a t t g t g t a c c t t t a g c t t t g t g t c c g t a t c t c a c a g a c g a t a a t t a t t g a t a c g a g t t t t t a a c a c a t g g a a a t c g a a a

5100 (in frame with SV40 NLS) LICKGVNKEYLMYSALTRDH LICKGVNKEYLMYSALTRDH t t a a t t t g t a a a g g g g t t a a t a a g g a a t a t t t g a t g t a t a g t g c c t t g a c t a g a g a t c a t a a t t a a a c a t t t c c c c a a t t a t t c c t t a t a a a c t a c a t a t c a c g g a a c t g a t c t c t a g t a

5160 (in frame with SV40 NLS) NQPYHICRGFTCFKKPPTPP NQPYHICRGFTCFKKPPTPP a a t c a g c c a t a c c a c a t t t g t a g a g g t t t t a c t t g c t t t a a a a a a c c t c c c a c a c c t c c c t t a g t c g g t a t g g t g t a a a c a t c t c c a a a a t g a a c g a a a t t t t t t g g a g g g t g t g g a g g g

HpaI 5220 (in frame with SV40 NLS) PEPET* SV40 poly(A) signal PEPET* c c t g a a c c t g a a a c a t a a a a t g a a t g c a a t t g t t g t t g t t a a c t t g t t t a t t g c a g c t t a g g a c t t g g a c t t t g t a t t t t a c t t a c g t t a a c a a c a a c a a t t g a a c a a a t a a c g t c g a a t

5280 SV40 poly(A) signal C G T T A T C G T A G T G T T T A A A G SV40pA-R t a a t g g t t a c a a a t a a a g c a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t a t t a c c a a t g t t t a t t t c g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a

5340 SV40 poly(A) signal G T G G T T T G T C C A A A C T C A T C EBV-rev g c a t t c t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t a t c a t g t c t g g a t c c c g t t c g t a a g a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a t a g t a c a g a c c t a g g g c a a

MluIPmeI 5400 t a a a c t a c g c g t a a t t c a a a c a g g g t t c t g g c g t c g t t c t c g t a c t g t t t t c c c c a g g c c a t t t g a t g c g c a t t a a g t t t g t c c c a a g a c c g c a g c a a g a g c a t g a c a a a a g g g g t c c g g

5460 1 5 10 signal sequence MSIQHFRVAL AmpR MSIQHFRVAL a g t g c t t t a g c g t t a t t g a a a a a g g a a g a g t a t g a g t a t t c a a c a t t t c c g t g t c g c c c t t c a c g a a a t c g c a a t a a c t t t t t c c t t c t c a t a c t c a t a a g t t g t a a a g g c a c a g c g g g a

5520 15 20 signal sequence IPFFAAFCLPVFA 25 30 HPETLVK AmpR IPFFAAFCLPVFAHPETLVK t a t t c c c t t t t t t g c g g c a t t t t g c c t t c c t g t t t t t g c t c a c c c a g a a a c g c t g g t g a a a t a a g g g a a a a a a c g c c g t a a a a c g g a a g g a c a a a a a c g a g t g g g t c t t t g c g a c c a c t t

5580 35 40 45 50 VKDAEDQLGARVGYIELDLN AmpR VKDAEDQLGARVGYIELDLN a g t a a a a g a t g c t g a a g a t c a g t t g g g t g c a c g a g t g g g t t a c a t c g a a c t g g a t c t c a a t c a t t t t c t a c g a c t t c t a g t c a a c c c a c g t g c t c a c c c a a t g t a g c t t g a c c t a g a g t t

5640 55 60 65 70 SGKILESFRPEERFPMMSTF AmpR SGKILESFRPEERFPMMSTF c a g c g g t a a g a t c c t t g a g a g t t t t c g c c c c g a a g a a c g t t t t c c a a t g a t g a g c a c t t t g t c g c c a t t c t a g g a a c t c t c a a a a g c g g g g c t t c t t g c a a a a g g t t a c t a c t c g t g a a a

5700 75 80 85 90 KVLLCGAVLSRIDAGQEQLG AmpR C G A T A C A C C G C G C C A T A A T A Amp-R KVLLCGAVLSRIDAGQEQLG t a a a g t t c t g c t a t g t g g c g c g g t a t t a t c c c g t a t t g a c g c c g g g c a a g a g c a a c t c g g a t t t c a a g a c g a t a c a c c g c g c c a t a a t a g g g c a t a a c t g c g g c c c g t t c t c g t t g a g c c

5760 95 100 105 110 RRIHYSQNDLVEYSPVTEKH AmpR RRIHYSQNDLVEYSPVTEKH t c g c c g c a t a c a c t a t t c t c a g a a t g a c t t g g t t g a g t a c t c a c c a g t c a c a g a a a a g c a a g c g g c g t a t g t g a t a a g a g t c t t a c t g a a c c a a c t c a t g a g t g g t c a g t g t c t t t t c g t

5820 115 120 125 130 LTDGMTVRELCSAAITMSDN AmpR LTDGMTVRELCSAAITMSDN t c t t a c g g a t g g c a t g a c a g t a a g a g a a t t a t g c a g t g c t g c c a t a a c c a t g a g t g a t a a a g a a t g c c t a c c g t a c t g t c a t t c t c t t a a t a c g t c a c g a c g g t a t t g g t a c t c a c t a t t

5880 135 140 145 150 TAANLLLTTIGGPKELTAFL AmpR TAANLLLTTIGGPKELTAFL c a c t g c g g c c a a c t t a c t t c t g a c a a c g a t c g g a g g a c c g a a g g a g c t a a c c g c t t t t t t g t g a c g c c g g t t g a a t g a a g a c t g t t g c t a g c c t c c t g g c t t c c t c g a t t g g c g a a a a a a

5940 155 160 165 170 HNMGDHVTRLDRWEPELNEA AmpR HNMGDHVTRLDRWEPELNEA g c a c a a c a t g g g g g a t c a t g t a a c t c g c c t t g a t c g t t g g g a a c c g g a g c t g a a t g a a g c c g t g t t g t a c c c c c t a g t a c a t t g a g c g g a a c t a g c a a c c c t t g g c c t c g a c t t a c t t c g

6000 175 180 185 190 IPNDERDTTMPVAMATTLRK AmpR IPNDERDTTMPVAMATTLRK c a t a c c a a a c g a c g a g c g t g a c a c c a c g a t g c c t g t a g c a a t g g c a a c a a c g t t g c g c a a g t a t g g t t t g c t g c t c g c a c t g t g g t g c t a c g g a c a t c g t t a c c g t t g t t g c a a c g c g t t

6060 195 200 205 210 LLTGELLTLASRQQLIDWME AmpR LLTGELLTLASRQQLIDWME a c t a t t a a c t g g c g a a c t a c t t a c t c t a g c t t c c c g g c a a c a a t t a a t a g a c t g g a t g g a t g a t a a t t g a c c g c t t g a t g a a t g a g a t c g a a g g g c c g t t g t t a a t t a t c t g a c c t a c c t

BglINmeAIII 6120 215 220 225 230 ADKVAGPLLRSALPAGWFIA AmpR ADKVAGPLLRSALPAGWFIA g g c g g a t a a a g t t g c a g g a c c a c t t c t g c g c t c g g c c c t t c c g g c t g g c t g g t t t a t t g c c c g c c t a t t t c a a c g t c c t g g t g a a g a c g c g a g c c g g g a a g g c c g a c c g a c c a a a t a a c g

6180 235 240 245 250 DKSGAGERGSRGIIAALGPD AmpR DKSGAGERGSRGIIAALGPD t g a t a a a t c t g g a g c c g g t g a g c g t g g g t c t c g c g g t a t c a t t g c a g c a c t g g g g c c a g a a c t a t t t a g a c c t c g g c c a c t c g c a c c c a g a g c g c c a t a g t a a c g t c g t g a c c c c g g t c t

AhdI 6240 255 260 265 270 GKPSRIVVIYTTGSQATMDE AmpR GKPSRIVVIYTTGSQATMDE t g g t a a g c c c t c c c g t a t c g t a g t t a t c t a c a c g a c g g g g a g t c a g g c a a c t a t g g a t g a a c c a t t c g g g a g g g c a t a g c a t c a a t a g a t g t g c t g c c c c t c a g t c c g t t g a t a c c t a c t

6300 275 280 285 RNRQIAEIGASLIKHW* AmpR RNRQIAEIGASLIKHW* a c g a a a t a g a c a g a t c g c t g a g a t a g g t g c c t c a c t g a t t a a g c a t t g g t a a c t g t c a g a t g c t t t a t c t g t c t a g c g a c t c t a t c c a c g g a g t g a c t a a t t c g t a a c c a t t g a c a g t c t

6360 c c a a g t t t a c t c a t a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t g g t t c a a a t g a g t a t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a

6420 c t a g g t g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t g a t c c a c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a

6480 ori c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a

6540 ori g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g

6600 ori g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g

6660 ori a a a t a c t g t c c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c t t t a t g a c a g g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g

6720 ori g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g

6780 ori g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c

6840 ori a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t

6900 ori c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t

6960 ori G G G A A A C G C pBR322ori-F t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g

DrdI 7020 ori C T G G T A T C T T T pBR322ori-F c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c

7080 ori a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a

7140 c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a

7200 g g a t a a c c g t a t t a c c g c c t t t g a g t g a g c t g a t a c c g c t c g c c g c a g c c g a a c g a c c g a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c g a c t a t g g c g a g c g g c g t c g g c t t g c t g g c t

End (7233) 3ʹ 5ʹ 7233 A G C G A G T C A G T G A G C G A G L4440 g c g c a g c g a g t c a g t g a g c g a g g a a g c g g a a g a c g c g t c g c t c a g t c a c t c g c t c c t t c g c c t t c t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.