Addgene pICH41258 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pICH41258 Sequencing Result


Map View

1000 750 500 250 End (1077) BtgI (1016) TaqII (922) SspI (859) BciVI (827) AatII (745) ZraI (743) pBRforEco (686 .. 704) BssSI - BssSαI (688) EcoO109I (684) SapI - BspQI (673) PflMI (662) AcuI - Eco57MI (654) DrdI (599) pRS-marker (525 .. 544) TatI (523) BaeGI - Bme1580I (517) ApaLI (513) BstAPI (512) NdeI (508) PluTI - HaeII (459) SfoI (457) NarI (456) KasI (455) BglI (446) FspI (436) PvuI - BsiEI (417) BmrI (336) M13/pUC Forward (309 .. 331) M13 Forward (300 .. 317) ApoI - EcoRI (294) SacI - BanII (292) Eco53kI (290) KpnI (286) Acc65I (282) SmaI (280) XmaI - TspMI (278) MflI * - BstYI - BamHI (273) XbaI (267) HincII (263) AccI (262) SalI (261) PstI - SbfI (259) SfcI (255) NspI - SphI (253) BfuAI - BspMI (248) HindIII (243) M13 Reverse (214 .. 230) M13/pUC Reverse (195 .. 217) Start (0) CAP binding site lac promoter lac operator lacZ α AmpR promoter M13 rev MCS M13 fwd pICH41258 1077 bp

Sequence View

Start (0) 60 c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t c a c t c t g t g g t c t c a a a t g t t g t c t t c g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a g t g a g a c a c c a g a g t t t a c a a c a g a a g

120 CAP binding site g c a g c t g g c a c g a c a g g t t t c c c g a c t g g a a a g c g g g c a g t g a g c g c a a c g c a a t t a a t g c g t c g a c c g t g c t g t c c a a a g g g c t g a c c t t t c g c c c g t c a c t c g c g t t g c g t t a a t t a c

180 -35 -10 CAP binding site lac promoter t g a g t t a g c t c a c t c a t t a g g c a c c c c a g g c t t t a c a c t t t a t g c t t c c g g c t c g t a t g t a c t c a a t c g a g t g a g t a a t c c g t g g g g t c c g a a a t g t g a a a t a c g a a g g c c g a g c a t a c a

240 1 5 MTMIT lac promoter lac operator lacZ α M13 rev A G C G G A T A A C A A T T T C A C A C A G G M13/pUC Reverse C A G G A A A C A G C T A T G A C M13 Reverse t g t g t g g a a t t g t g a g c g g a t a a c a a t t t c a c a c a g g a a a c a g c t a t g a c c a t g a t t a c g a c a c a c c t t a a c a c t c g c c t a t t g t t a a a g t g t g t c c t t t g t c g a t a c t g g t a c t a a t g c

HindIIIBspMIBfuAISphINspISfcISbfIPstISalIAccIHincIIXbaIBamHIBstYIMflI*TspMIXmaISmaIAcc65IKpnIEco53kIBanIISacIEcoRIApoI 300 10 15 20 25 PSLHACRSTLEDPRVPSSNS lacZ α MCS M13 fwd T M13 Forward c c a a g c t t g c a t g c c t g c a g g t c g a c t c t a g a g g a t c c c c g g g t a c c g a g c t c g a a t t c a g g t t c g a a c g t a c g g a c g t c c a g c t g a g a t c t c c t a g g g g c c c a t g g c t c g a g c t t a a g t

BmrI 360 30 35 40 45 LAVVLQRRDWENPGVTQLNR lacZ α M13 fwd G A C C G G C A G C A A A A T G T M13 Forward G C A A A A T G T T G C A G C A C T G A C C C M13/pUC Forward c t g g c c g t c g t t t t a c a a c g t c g t g a c t g g g a a a a c c c t g g c g t t a c c c a a c t t a a t c g c g a c c g g c a g c a a a a t g t t g c a g c a c t g a c c c t t t t g g g a c c g c a a t g g g t t g a a t t a g c g

BsiEIPvuI 420 50 55 60 65 LAAHPPFASWRNSEEARTDR lacZ α c t t g c a g c a c a t c c c c c t t t c g c c a g c t g g c g t a a t a g c g a a g a g g c c c g c a c c g a t c g c g a a c g t c g t g t a g g g g g a a a g c g g t c g a c c g c a t t a t c g c t t c t c c g g g c g t g g c t a g c g

KasINarISfoIHaeIIPluTIBglIFspI 480 70 75 80 85 PSQQLRSLNGEWRLMRYFLL lacZ α c c t t c c c a a c a g t t g c g c a g c c t g a a t g g c g a a t g g c g c c t g a t g c g g t a t t t t c t c c t t g g a a g g g t t g t c a a c g c g t c g g a c t t a c c g c t t a c c g c g g a c t a c g c c a t a a a a g a g g a a

NdeIBstAPIApaLIBme1580IBaeGITatI 540 90 95 100 105 THLCGISHRIWCTLSTICSD lacZ α A T G T T A G A C G A G A C T A pRS-marker a c g c a t c t g t g c g g t a t t t c a c a c c g c a t a t g g t g c a c t c t c a g t a c a a t c t g c t c t g a t t g c g t a g a c a c g c c a t a a a g t g t g g c g t a t a c c a c g t g a g a g t c a t g t t a g a c g a g a c t a

DrdI 600 AA* lacZ α C G G C pRS-marker g c c g c a t a g t t a a g c c a g c c c c g a c a c c c g c c a a c a c c c g c t g a c g c g c c c t g a c g g g c t c g g c g t a t c a a t t c g g t c g g g g c t g t g g g c g g t t g t g g g c g a c t g c g c g g g a c t g c c c g a

Eco57MIAcuI 660 t g t c t g c t c c c g g c a t c c g c t t a c a g a c a a g c t g t g a c g a a g a c a a a g g t t g a g a c c a c g a c a g a c g a g g g c c g t a g g c g a a t g t c t g t t c g a c a c t g c t t c t g t t t c c a a c t c t g g t g c

EcoO109IBssSαIBssSIBspQISapIPflMI 720 C G G A G C A C T A T G C G G A T A A pBRforEco a a g t g g c t c t t c a g t g g a c g a a a g g g c c t c g t g a t a c g c c t a t t t t t a t a g g t t a a t g t c t t c a c c g a g a a g t c a c c t g c t t t c c c g g a g c a c t a t g c g g a t a a a a a t a t c c a a t t a c a g

ZraIAatII 780 AmpR promoter a t g a t a a t a a t g g t t t c t t a g a c g t c a g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c t a c t a t t a t t a c c a a a g a a t c t g c a g t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g

BciVI 840 AmpR promoter c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g

SspI 900 AmpR promoter t g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t a t g c g c t c a c g c a a c t g g t c c a g a a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a t a c g c g a g t g c g t t g a c c a g g t c t

TaqII 960 a c c t t g a c c g a a c g c a g c g g t g g t a a c g g c g c a g t g g c g g t t t t c a t g g c t t g t t a t g a c t g g a a c t g g c t t g c g t c g c c a c c a t t g c c g c g t c a c c g c c a a a a g t a c c g a a c a a t a c t g

BtgI 1020 t g t t t t t t t g g g g t a c a g t c t a t g c c t c g g g c a t c c a a g c a g c a a g c g c g t t a c g c c g t g a c a a a a a a a c c c c a t g t c a g a t a c g g a g c c c g t a g g t t c g t c g t t c g c g c a a t g c g g c a c

End (1077) 1077 g g t c g a t g t t t g a t g t t a t g g a g c a g c a a c g a t g t t a c g c a g c a g g g c a g t c g c c c t c c a g c t a c a a a c t a c a a t a c c t c g t c g t t g c t a c a a t g c g t c g t c c c g t c a g c g g g a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.